Determination of Tryptophan in Bee Pollen and Royal Jelly by High-Performance Liquid Chromatography with Fluorescence DetectionBiomedical Chromatography, 2009 Apr 27
A method for tryptophan analysis in bee pollen and royal jelly was developed using HPLC with fluorescence detection.
To determine the free tryptophan in bee pollen and royal jelly, ultrasonic extraction was performed using water (pH 6.3)-acetonitrile (10:1, v/v) as extraction solvent. While determining the total tryptophan in these bee products, the method involves alkaline hydrolysis of the proteins with 4 mol/L sodium hydroxide at 110 degrees C for 20 h under anaerobic conditions…
A linear response (r(2 )> 0.9998) was obtained in the range 0.0625-5.0 microg/mL. The method was successfully applied to the determination of the free and total tryptophan contents in bee pollens, which were 0.069 +/- 0.003 and 2.693 +/- 0.476 mg/g, respectively, while only the total tryptophan was detected in royal jelly, with a content of 1.743 +/- 0.066 mg/g.
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