New Method to Measure Energy Transfer Molecules in Royal Jelly

Online Cleanup of Accelerated Solvent Extractions for Determination of Adenosine 5′-Triphosphate (ATP), Adenosine 5′-Diphosphate (ADP), and Adenosine 5′-Monophosphate (AMP) in Royal Jelly Using High-Performance Liquid Chromatography
J. Agric. Food Chem, May 12, 2009

Determination of the levels of adenosine 5′-triphosphate (ATP), adenosine 5′-diphosphate (ADP), and adenosine 5′-monophosphate (AMP) in royal jelly is important for the study of its pharmacological activities, health benefits, and adenosine phosphate degradation.

In this study was developed a novel method to determine ATP, ADP, and AMP levels in royal jelly using accelerated solvent extraction (ASE) followed by online cleanup and high-performance liquid chromatography (HPLC) with diode array detection (DAD)…

With this ASE-HPLC method, a minisurvey of ATP, ADP, and AMP levels in 15 samples of royal jelly of different origins was performed. Sample results indicated that the AMP concentration was 24.2−2214.4 mg kg−1, whereas ATP and ADP were not detectable or present only at low levels.