Comparative Study of Different Portuguese Samples of Propolis:
Pollinic, Sensorial, Physicochemical, Microbiological Characterization and Antibacterial
Activity
Food Chem Toxicol, 2012 Sep 5
The aim of this work was to study four propolis samples from
Trás-os-Montes region of Portugal. The propolis samples' color was different,
which pollen analysis showed to be due to different botanical sources: Populus
sp., Pinus sp., Quercus sp. and Castanea sativa. The data from physicochemical
analysis (moisture, soluble and insoluble solids content, pH, conductivity, ash
content, wax, total phenolics and flavonoids content) was treated using
multivariate statistical tools as cluster heat map, principal components
analysis and linear discriminant analysis with the purpose of classifying the
sample accordingly to the botanical/geographical origin.
The discriminant analysis was applied with stepwise to
select the variables that most contribute to sample identification accordingly
to pollinic profile. The cross-validation technique was applied, using the
leave-one-out procedure, which showed good prediction capabilities of the
samples. Microbiologically, the commercial quality was satisfactory, since the
samples didn't contain deterioration or pathogenic microorganisms.
All the samples studied presented antimicrobial activity
against Methicillin-resistant Staphylococcus aureus, in a dose dependent way.
The antimicrobial activity was strictly related to the physicochemical
composition. This work will allow connecting a particular chemical propolis
type to a specific type of biological activity, what is essential for the use
in therapeutic applications.
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