Development and Validation of HPLC Method for Determination
of Salicin in Poplar Buds: Application for Screening of Counterfeit Propolis
Oct. 22-25, 2012
Zhenjiang, China
The main plant origins of propolis are the populus species
and their hybrids, both located in China. Poplar tree gum, the extract of populus
buds, has been widely used as counterfeit propolis, but no effective method was
known for detecting the counterfeit.
Salicin is a characteristic marker of the genus populus,
which may be hydrolyzed by beta-glucosidase during propolis collection and
processing. A simple, sensitive and specific reverse-phase high-performance
liquid chromatography (RP-HPLC) method was developed and validated for the
rapid assay of salicin, which was aimed at distinguishing poplar tree gum from
propolis.
Following this method, salicin was detected in populus buds,
leaves and 11 poplar tree gum samples, but not in any of the 40 propolis
samples, which indicated that salicin was hydrolyzed in propolis collection and
processing, but was stable in the production process of poplar tree gum.
The proposed method could be an effective technique for routine
analysis of salicin and monitoring the quality of propolis.
For information, contact: HU Fu-Liang, College of Animal
Sciences, Zhejiang University, Hangzhou 310058, China E-mail: flhu@zju.edu.cn
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