Apimedica: Polyphenolic Content and Pharmacological Potential of French BFA Propolis Extracts - 法国BFA 蜂胶提取物的多酚类物质及药理作用潜能

Apimondia Apimedica-Apiquality International Forum

Oct. 22-25, 2012

Zhenjiang, China

Séverine BOISARD^a, Anne-Marie Le Ray^a, Julia Gatto^a, Marie-Christine Aumond^a, Anne Landreau^a, Marie Kempf^b, Isabelle Péruches^c, Catherine Flurin^c and Pascal Richomme^a

^aPRES LUNAM, Université d’Angers, EA 921 SONAS, Angers, France, severine.boisard@etud.univ-angers.fr; ^b CHU d’Angers, Laboratoire de Bactériologie, Angers, France; ^cBallot-Flurin Apiculteurs, La miellerie, Lahitte-Toupière, France

Propolis, or bee glue, is a natural resinous hive product collected by honeybees from buds and exudates of various trees and plants. Mixed with beewax and salivary enzymes, it is employed to fill cracks and embalm dead invaders in the hive. Propolis has been used in folk medecine since ancien times due to its pharmacological potential associated with antioxidant, antifungal, antibacterial as well as antitumoral properties. A batch of various French propolis extracts, supplied by “Ballot-Flurin Apiculteurs” (BFA), a company located in the South-West of France and specialized in apitherapy products, was fractionated and analysed by HPLC/MS. Its qualitative chemical composition highlights the presence of polyphenols such as hydroxycinnamic acid derivatives and flavonoids. Total polyphenol content and antioxidant activities were evaluated on six BFA propolis extracts, using respectively Folin-Ciocalteu, DPPH and ORAC assays. Preliminary antifungal (Candida albicans) and antibacterial (Staphylococcus aureus) evaluations will also be given.

Key words: propolis, polyphenols, biological assays

法国BFA 蜂胶提取物的多酚类物质及药理作用潜能

Severine Boisard1, Anne-Marie Le Ray1, Julia Gatto1 , Marie-Christine Aumond1, Anne Landreau1, Marie Kempf2,

Isabelle Peruches3, Catherine Flurin3, Pascal Richomme1 （法国）

(1. Pres Lunam, Universite d’Angers, EA 921 Sonas, Angers, France, ;

2. Chu d’Angers, Laboratoire de Bacteriologie, Angers, France;

3. Ballot-Flurin Apiculteurs, La miellerie, Lahitte-Toupiere, France)

蜂胶是一种蜜蜂从多种树木及植物的嫩芽及渗出物中采集得到的天然树脂状蜂产品。同蜂蜡和唾液酶 混合，蜜蜂将它用于填补蜂巢的缝隙及包裹入侵者的尸体。蜂胶具有良好的药理学活性，包括抗氧化、抗 真菌、抗细菌及抗肿瘤活性等，故自古以来就被用于民间医药。我们对一批由Ballot-Flurin Apiculteurs（BFA）公司（位于法国西南，专注于蜂疗产品的开发）提供的一批蜂胶提取物样品进行了分馏，并利用HPLC/MS 分析了其主要成分。定量分析结果表明BFA 蜂胶中富含大量的多酚类化合物，如羟基肉桂酸衍生物、黄酮 类化合物等。我们主要采用福林酚法、DPPH 和ORAC 法对六个BFA 蜂胶提取物的多元酚含量及其抗氧化 活性进行了研究。我们也初步评价了样品的抗真菌（白色念珠菌）、抗细菌（金黄色葡萄球菌）的活性

关键词：蜂胶；多酚类化合物；生物学测定