Apimedica: Bee Pollen is an Effective Natural Anti-Oxidant - 玉米蜂花粉油脂抗氧化性研究及成分分析

Apimondia Apimedica-Apiquality International Forum

Oct. 22-25, 2012

Zhenjiang, China

Antioxidant Activity and Composition Analysis of Corn Bee Pollen Oil

SUN Li-ping*, XU Xiang, HE Wei

(Bee Research Institution, The Chinese Academy of Agricultural Sciences, Beijing 100093，China)

The capacity of bee pollen oil is evaluated by these three indicators: ABTS, DPPH radical scavenging and the phosphomolybdate complex. The content of carotenoid is determined by spectrophotometry, and the phospholipid composition is quantitatively analyzed by HPLC. The results show that corn bee pollen oils have strong scavenging ability on the ABTS, DPPH free radicals, and the IC50 values are 0.26±0.01mg/mL，2.20±0.05mg/mL respectively. A strong antioxidant capacity has also identified by the Phosphomolybdate complex method. Corn Bee Pollen, in which carotenoid content is 16.21 ± 0.84 mg/100g and the total phospholipid content is 2.98 ± 0.02g/100g, contains five phospholipid composition, namely, PI, PS, PE, PC, LPC. The proportion of carotenoids and total phospholipid in grease is 0.21% and 38.11%. Finally, the conclusion is drawn that corn bee pollen contains a variety of fat-soluble antioxidant active ingredients, can supply hydrogen or electrons, and is an effective natural anti-oxidant resources.

Key words: corn and bee pollen; antioxidant; fat-soluble components; content;

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*Corresponding author,E-mail：caasum@126com.cn

玉米蜂花粉油脂抗氧化性研究及成分分析

孙丽萍*，徐 响，何 伟

（中国农业科学院蜜蜂研究所，北京 100093，中国）

以ABTS、DPPH 自由基清除和磷钼络合物法三个指标评价蜂花粉油脂的抗氧化能力，通过分光光度法 测定类胡萝卜素含量，HPLC 法定量分析其中的磷脂成分。结果表明，玉米蜂花粉油脂对ABTS、DPPH 自由基有较强的清除能力，IC50 值分别为0.26±0.01mg/mL，2.20±0.05mg/mL，在磷钼络合物法中也表现出较 强的抗氧化能力。玉米蜂花粉中类胡萝卜素含量为16.21±0.84 mg/100g 花粉，总磷脂含量为2.98± 0.02g/100g 花粉，含有PI、PS、PE、PC、LPC 五种磷脂成分。类胡萝卜素和总磷脂含量占油脂的比例为0.21% 和38.11%。结论：玉米蜂花粉含有多种脂溶性抗氧化活性成分，有很好的供应氢或电子的能力，是一种有 效的天然抗氧化剂资源。

关键词：玉米蜂花粉；抗氧化；脂溶性成分；含量