Molecules 2013, 18(2), 2200-2211
The present study was designed to determine the
radioprotective effects of Malaysian Gelam honey on gene expression and enzyme
activity of superoxide dismutase (SOD), catalase (CAT) and glutathione
peroxidase (GPx) of human diploid fibroblasts (HDFs) subjected to
gamma-irradiation.
Six groups of HDFs were studied: untreated control,
irradiated HDFs, Gelam honey-treated HDFs and HDF treated with Gelam honey
pre-, during- and post-irradiation. HDFs were treated with 6 mg/mL of
sterilized Gelam honey (w/v) for 24 h and exposed to 1 Gray (Gy) of gamma rays
at the dose rate of 0.25 Gy/min. Gamma-irradiation was shown to down-regulate
SOD1, SOD2, CAT and GPx1 gene expressions (p < 0.05). Conversely, HDFs
treated with Gelam honey alone showed up-regulation of all genes studied.
Similarly, SOD, CAT and GPx enzyme activities in HDFs decreased with
gamma-irradiation and increased when cells were treated with Gelam honey (p
< 0.05). Furthermore, of the three different stages of study treatment,
pre-treatment with Gelam honey caused up-regulation of SOD1, SOD2 and CAT genes
expression and increased the activity of SOD and CAT.
As a conclusion, Gelam honey modulates the expression of
antioxidant enzymes at gene and protein levels in irradiated HDFs indicating
its potential as a radioprotectant agent.
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