Journal of the Science of Food and Agriculture, Accepted
Article
Background
Propolis is a complex resinous sticky substance that
honeybees collect from buds and exudates of various plants. Due to propolis
versatile biological and pharmacological activities, it is widely used in
medicine, cosmetics and food industry. The aim of this study was to evaluate
cytotoxic and antioxidative effects of various ethanolic extracts of propolis
(EEP) on human colon cancer cell line (HCT-116) and compare it with their
composition. HPLC-DAD method was used to determine the chemical composition of
propolis samples.
Results
Results
The most abundant flavonoids in all samples were chrysin,
pinocembrin and galangin (12.697- 40.811 µg mg-1). On the other hand, main
phenolic acids were caffeic, ferulic and isoferulic acid. Dose and
time-dependent inhibition of cell growth of HCT-116 cells was observed in all
propolis samples, with IC50 values ranging from 26.33 to 143.09 µg mL-1.
Differences in cytotoxic activity of propolis samples were associated with
differences in their composition. Our results showed that all EEP samples
reduced both superoxide anion radical and nitrite levels and also had strong
DPPH scavenging activity.
Conclusion
All tested propolis samples had pronounced cytotoxic and
antioxidative activities.
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