Mol Med Rep, 2013 Mar 20
In the present study, bee pollen polysaccharides from Rosa
rugosa (WRPP) were extracted and fractionated. WRPP were purified to neutral
(WRPP-N) and acidic polysaccharides (WRPP-1, WRPP-2) with DEAE-Cellulose.
WRPP-N were mainly composed of glucose, mannose, arabinose
and galactose, indicating the existence of glucan, arabinogalactan (AG) and
mannoglucan. WRPP-1 mainly consisted of rhamnose (3.0%), galacturonic acid
(12.4%), galactose (24.7%) and arabinose (53.9%), and contained a large
proportion of AGs. WRPP-2 consisted of rhamnose (7.8%), galacturonic acid
(23.0%), galactose (15%) and arabinose (48.7%), while WRPP-2 contained more
galacturonic acid compared to WRPP-1. WRPP-1 and WRPP-2 were composed by type I
rhamnogalacturonan (RG-I), homogalacturonan (HG) and AG fragments, while WRPP-2
contained more HG and RG-I.
All the fractions had significant anti-proliferative
activity in HT-29 and HCT116 cells; the neutral and acidic fractions were shown
to have significant synergistic effects which accounted for the antitumor
activity of bee pollen polysaccharides from Rosa rugosa in vitro.
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