Arch Med Res, 2013 May 15. pii: S0188-4409(13)00110-0
BACKGROUND AND AIMS:
Antibiotic multiresistant microbes represent a challenging
problem. Because honey has a potent antibacterial property, the antimicrobial
effects of different honey samples against multiresistant pathogens and their
compositions were investigated.
METHODS:
Five honey samples were used: Talah, Dhahian, Sumra-1, Sidr,
and Sumra-2. Samples were analyzed to determine chemical composition such as
fructose, glucose, sucrose, pH, total flavonoids, total phenolics, hydrogen
peroxide concentration, minerals and trace elements. Antimicrobial activities
of the samples against 17 (16 were multiresistant) human pathogenic bacteria
and three types of fungi were studied. Specimens of the isolates were cultured
into 10 mL of 10-100% (volume/volume) honey diluted in broth. Microbial growth
was assessed on a solid plate media after 24 h and 72 h incubation.
RESULTS:
The composition of honey samples varied considerably. Sumra
1 and 2 contained the highest level of flavonoids and phenolics and the lowest
level of hydrogen peroxide, whereas Dhahian honey contained the highest level
of hydrogen peroxide. Sixteen pathogens were antibiotic multiresistant. A
single dose of each honey sample inhibited all the pathogens tested after 24 h
and 72 h incubation. The most sensitive pathogens were Aspergillus nidulans,
Salmonella typhimurum and Staphylococcus epidermidis (S. epidermidis). Although
there was no statistically significant difference in the effectiveness of honey
samples, the most effective honey against bacteria was Talah and against fungi
were Dhahian and Sumra-2.
CONCLUSIONS:
Various honey samples collected from different geographical
areas and plant origins showed almost similar antimicrobial activities against
multiresistant pathogens despite considerable variation in their composition.
Honey may represent an alternative candidate to be tested as part of management
of drug multiresistant pathogens.
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