In Vitro Antimicrobial Activity of Ethanolic Extract of
Polish Propolis against Biofilm Forming Staphylococcus epidermidis Strains
Evid Based Complement Alternat Med, 2013;2013:590703, Epub
2013 Apr 10
The aim of the presented study was to examine the
antimicrobial activity of ethanol extract of Polish propolis (EEPP) against
biofilm-forming CoNS strains in vitro.
Our results revealed that EEPP displayed varying degrees of
activity against CoNS with MIC values ranging from 1.56 to 0.78 mg/mL. The
average MIC was 1.13 ± 0.39 mg/mL while calculated MIC50 and MIC90 values were
0.78 mg/mL and 1.56 mg/mL, respectively. The biofilm formation ability by all
tested S. epidermidis strains was inhibited at EEPP concentrations ranging from
0.39 to 1.56 mg/mL.
The degree of reduction of AlamarBlue was directly
associated with the proliferation of S. epidermidis strains. The increased
proliferation of S. epidermidis strains was observed after 12 and 24 hours of
incubation in the presence of EEPP concentrations ranging from 0.025 to
0.39 mg/mL.
These results suggest that antimicrobial activities of EEPP
against S. epidermidis expressed as the reduction of bacterial growth,
reduction of biofilm formation ability, and the intensity of proliferation were
significantly affected by incubation time and EEPP concentration used as well
as the interactions between these factors.
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