Caffeic Acid Phenethyl Ester Inhibits Endothelial Tissue
Factor Expression
Biol Pharm Bull, 2013;36(6):1032-1035
Caffeic acid phenethyl ester (CAPE) is a component of
honeybee hives with various beneficial properties. Tissue factor (TF), the key
trigger of thrombosis, is expressed in human endothelial cells.
This study was designed to investigate whether CAPE
modulates TF expression in human aortic endothelial cells (HAECs).
Western blots and real-time polymerase chain reactions were
performed. CAPE (10-7-10-5 m) inhibited tumor necrosis factor (TNF)-α induced
endothelial TF protein expression by 2.1-fold at 10-5 m (p < 0.0001).
Similarly, TF surface activity was reduced (p < 0.02). In contrast, TF mRNA
expression, TF promoter activity, and mitogen-activated protein (MAP) kinase
activation remained unaltered.
In conclusion, CAPE inhibits TF protein expression and
activity at the posttranscriptional level thereby exhibiting anti-thrombotic
potential.
No comments:
Post a Comment