Effects of honeybee (Apis mellifera) venom on keratinocyte
migration in vitro
Pharmacogn Mag, 2013 Jul;9(35):220-6
BACKGROUND:
Since the ancient times the skin aging application of
honeybee venom (BV) is practiced and persisted until nowadays. The present
study evaluated the effect of the honeybee venom (BV) on keratinocyte migration
in wound healing model in vitro.
OBJECTIVE:
To access BV further as a cosmetic ingredient and a potential external application for topical uses, we performed studies to investigate the biologic effect of BV treatment on keratinocyte proliferation and migration in vitro.
OBJECTIVE:
To access BV further as a cosmetic ingredient and a potential external application for topical uses, we performed studies to investigate the biologic effect of BV treatment on keratinocyte proliferation and migration in vitro.
MATERIAL AND METHODS:
BV cytotoxicity was assessed by using a
3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay over
24 h. To assess BV genotoxicity, damage to human epidermal keratinocyte (HEK)
was evaluated using the Comet assay. HEK migration was evaluated using a
commercial wound healing kit. The skin pro-inflammatory cytokines interleukin
(IL)-8 and tumor necrosis factor (TNF)-α were examined to evaluate the
pro-inflammatory response to BV.
RESULTS:
It was found that BV was not cytotoxic and stimulated more HEK proliferation and migration compared to negative control, and did not induce DNA damage. There were also decreases in IL-8 and TNF-α expression levels in HEK at all time points.
CONCLUSION:
These findings highlight the potential of topical
application of BV for promoting cell regeneration and wound treatment.
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