Anal. Methods, 2013
Although royal jelly constitutes one of the richest natural
sources of pantothenic acid (vitamin B5), a reliable and validated
chromatographic method to determine this analyte in this matrix has yet to be
described in literature. In this work we present an original RP-HPLC procedure
to measure the concentration of pantothenic acid in royal jelly. A sample
pre-treatment is needed to prevent the interference of high protein
concentration in the matrix.
The method has been validated in terms of LoD,
LoQ, linearity, precision (repeatability and reproducibility) and bias.
Finally, the whole procedure was tested on a number of samples of royal jelly
from different origin, providing concentration values spanning from 120±30 to
565±40 mg Kg-1 of pantothenic acid.
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