CAPE promotes the expansion of human umbilical cord
blood-derived hematopoietic stem and progenitor cells in vitro
Sci China Life Sci, 2014 Jan 22
Due to the low number of collectable stem cells from single
umbilical cord blood (UCB) unit, their initial uses were limited to pediatric
therapies. Clinical applications of UCB hematopoietic stem and progenitor cells
(HSPCs) would become feasible if there were a culture method that can
effectively expand HSPCs while maintaining their self-renewal capacity. In
recent years, numerous attempts have been made to expand human UCB HSPCs in
vitro.
In this study, we report that caffeic acid phenethyl ester (CAPE), a
small molecule from honeybee extract, can promote in vitro expansion of HSPCs.
Treatment with CAPE increased the percentage of HSPCs in cultured mononuclear
cells. Importantly, culture of CD34+ HSPCs with CAPE resulted in a significant
increase in total colony-forming units and high proliferative potential
colony-forming units. Burst-forming unit-erythroid was the mostly affected
colony type, which increased more than 3.7-fold in 1 μg mL-1 CAPE treatment
group when compared to the controls. CAPE appears to induce HSPC expansion by
upregulating the expression of SCF and HIF1-α.
Our data suggest that CAPE may
become a potent medium supplement for in vitro HSPC expansion.
No comments:
Post a Comment