Protective effects of propolis on cryopreservation of common
carp (Cyprinuscarpio) sperm
Available online 16 January 2014
Cryopreservation of sperm is common procedures in
aquaculture, particularly used for routine inartificial insemination. However,
these application cause damages and adversely affected spermmotility, viability
and consequently lower hatching rates. The objective of this study is to
determine whether propolis has an effect on cryopreservation and fertilization
ability and to investigate the potential protective effect of propolis on
spermatozoa of Cyprinus Carpio. Many studies have been done in cryopreservation
offish spermatozoa, but none of them contain propolis in extender composition.
The extenders were prepared by using modified Kurokura Solution to which 10%
Me2SO added with different levels of propolis (0.2, 0.4, 0.6, 0.8 and 1 mg
ml-1) and 10% egg yolk (as a control without propolis). The pooled semen
samples diluted at the ratio of 1:9 by the extenders were subjected to
cryopreservation. The percentage and duration of motility and fertlization
tests of cryopreserved sperm samples have been done immediately after thawing
and compared with control and fresh semen.
The extenders containing propolis exhibited higher
percentage motility andmotility duration than control group (P < 0.05).
Especially the group IV (0.8 mg ml-1 propolis) and the group V (1 mg ml-1 propolis)
showed significant positive effects on both post thaw motility and hatching
ability. The propolis maintained the integrity of the spermatozoa during the
cryopreservation process. Evaluating with its contents, it has been shown that
propolis is an appropriate cryoprotective agent in fish semen.
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