Effect of high pressure processing on the conversion of
dihydroxyacetone to methylglyoxal in New Zealand mānuka (Leptospermum scoparium)
honey and models thereof
Food Chem, 2014 Jun 15;153:134-9
The effect of high pressure processing (HPP) on the
conversion of dihydroxyacetone (DHA) to methylglyoxal (MGO) was examined in New
Zealand mānuka honey and models thereof.
The objective was to confirm that previously reported
increases of MGO with HPP treatment originated from conversion of DHA. RP-HPLC
was used to quantify DHA, MGO and hydroxymethylfurfural (HMF) after
derivatisation with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride
(PFBHA) or (in the case of MGO) separately with o-phenylenediamine (OPD). Fresh
and stored mānuka honey, clover honey with DHA added and artificial 26 honey
with DHA added were subjected to nine different pressures and holding times and
compared to untreated samples.
There was no consistent trend of decrease in DHA or increase
in MGO for any of the samples with any treatment. Samples showed random change
generally within 5-10% of an untreated sample for MGO, DHA and HMF. HPP does
not accelerate the conversion of DHA to MGO in honey.
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