Phenolic acids, hydrolyzable tannins and antioxidant
activity of geopropolis from the stingless bee Melipona fasciculata Smith
J Agric Food Chem, 2014 Feb 27
Geopropolis is a mixture of plant resins, waxes and soil
produced by the stingless bee Melipona fasciculata Smith. This paper describes
the antioxidant activity and chemical composition of geopropolis produced by
Melipona fasciculata. The total phenolic content determined by the
Folin-Ciocalteu reagent was highest in the ethyl acetate fraction and
hydroalcoholic extract. Antioxidant activity was assayed by the in vitro DPPH,
ABTS and FRAP assays. The hydroalcoholic extract and fractions of geopropolis,
except for the hexane fraction, exhibited antioxidant activity against DPPH,
ABTS and FRAP. The phenolic compounds were identified by HPLC-DAD-MS based on
the evaluation of their UV-vis absorption maxima (λmax) and mass spectral
analysis. Eleven compounds belonging to the classes of phenolic acids and
hydrolyzable tannins (gallotannins and ellagitannins) were tentatively
identified. These compounds are responsible for the antioxidant activity and
high phenolic content of geopropolis produced by M. fasciculata.
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