The Interaction of Bee Products With Temozolomide in Human
Diffuse Astrocytoma, Glioblastoma Multiforme and Astroglia Cell Lines
Nutr Cancer, 2014 Sep 25:1-10
In the present study, we investigated the influence of
extracts from Salix spp. honey (ESH), beebread (EBB), and royal jelly (ERJ)
with and without temozolomide (TMZ) on cell lines derived from a patient with
diffuse astrocytoma (DASC), human glioblastoma multiforme (U87MG), and normal
human astroglia (SVGp12). DASC was identified by immunocytochemistry. TMZ (20
μM) in combination with ESH (30 μg/mL), EBB (50 μg/mL), and ERJ (30 μg/mL) has
stronger cytotoxic activity on U87MG cells after 72 h (20.0, 26.5, and 29.3% of
control, respectively) than TMZ alone (about 6% of control). An increase of the
cytotoxic effect and inhibition of DNA synthesis in SVGp12 were detected after
administering TMZ with the studied extracts. NF-κB p50 subunit was reduced in
U87MG cells after treatment with ESH (70.9%) and ESH + TMZ (74.7%). A
significant decline of MMP-9 and MMP-2 secretion in cultured U87MG was detected
after incubation with EBB (42.9% and 73.0%, respectively) and EBB + TMZ (38.4%
and 68.5%, respectively).
In conclusion, the use of bee products may increase
the cytotoxic effect of TMZ in U87MG but also in SVGp12 cell line. It is
important to note that the U87MG cells were sensitive to natural bee products,
although there was no influence of natural bee products on the DASC cells.
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