Protective Effects of Gelam Honey against Oxidative Damage
in Young and Aged Rats
Volume 2014 (2014), Article ID 673628, 8 pages
Aging is characterized by progressive decline in
physiological and body function due to increase in oxidative damage. Gelam
honey has been accounted to have high phenolic and nonphenolic content to
attenuate oxidative damage. This study was to determine the effect of local
gelam honey on oxidative damage of aged rats. Twenty-four male Spraque-Dawley
rats were divided into young (2 months) and aged (19 months) groups. Each group
was further divided into control (fed with plain water) and supplemented with
2.5 mg/kg body weight of gelam honey for 8 months. DNA damage level was
determined by comet assay and plasma malondialdehyde (MDA) by high performance
liquid chromatography (HPLC). The activity of blood and cardiac antioxidant
enzymes was determined by spectrophotometer. The DNA damage and MDA level were
reduced in both gelam honey supplemented groups. Gelam honey increases
erythrocytes CAT and cardiac SOD activities in young and cardiac CAT activity
in young and aged groups. The DNA damage was increased in the aged group
compared to young group, but reduced at the end of the study. The decline of
oxidative damage in rats supplemented with gelam honey might be through the
modulation of antioxidant enzyme activities.
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