In-vitro screening of Malaysian honey from different floral
sources for antibacterial activity on human pathogenic bacteria
Afr J Tradit Complement Altern Med. 2014 Jan 28;11(2):315-8
BACKGROUND:
Different researches on therapeutic effects of honey have
been conducted in different regions; however the study on the potential
antibacterial activity of Malaysian honey is still limited. In this study,
antibacterial activities of different monofloral honey samples were tested
against several common human pathogenic bacteria.
MATERIALS AND METHODS:
The well-diffusion method, minimum inhibitory concentrations
(MIC) and minimum bactericidal concentration (MBC) techniques were employed to
investigate the putative antibacterial activity of Malaysian monofloral honey
from Koompassia excelsa (Becc.) Taub (Tualang), Melaleuca cajuputi Powell
(Gelam) and Durio zibethinus Murr. (Durian). Honey samples were tested against
Staphylococcus aureus ATCC6518 and ATCC25923, Staphylococcus epidermidis
ATCC12228, Enterococcus faecium LMG16192, Enterococcus faecalis LMG16216 and
ATCC29212, Escherichia coli ATCC25922, Salmonella enterica serovar Typhimurium
ATCC14028 and Klebsiella pneumoniae ATCC13883.
RESULTS:
Marked variations were observed in the antibacterial
activity of these honey samples. Durian honey failed to produce substantial
antibacterial activity, whereas Tualang and Gelam honey showed a spectrum of
antibacterial activity with their growth inhibitory effects against all of the
tested bacterial species including vancomycin-resistant enterococci (VRE).
CONCLUSION:
Present findings suggested Gelam honey possesses highest
antibacterial effect among the tested Malaysian honey samples.
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