Friday, October 19, 2018

Iranian Honey Bee Venom Component May Help Treat Cervical Cancer


Apoptotic Effect of Melittin Purified from Iranian Honey Bee Venom on Human Cervical Cancer HeLa Cell Line
International Journal of Peptide Research and Therapeutics
December 2018, Volume 24, Issue 4, pp 563–570

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Melittin, an amphipathic 26-residue peptide, is the main component of honey bee venom. Studies have been demonstrated that melittin has an inhibitory effect on proliferation of cancer cells. However, the precise mechanism of action is not completely understood. In the present study we have shown that purified melittin from Iranian honey bee venom shows anti-cancer effects on human cervical cancer cell line through induction of apoptosis. The venom was collected from Iranian honey bee (Apis mellifera meda) and melittin isolated using reversed phase HPLC. Biological activity of melittin was analyzed by hemolytic test on human red blood cells.

In order to investigate whether melittin inhibits proliferation of cervical cancer cells, the viability of the melittin treated HeLa cell line was measured via MTT assay. Finally, cell death analysis was performed using Propidum iodide and Annexin V-FITC dual staining. The results showed that the half hemolytic concentration (HD50) induced by mellitin was 0.5 µg/ml in free FBS solution. IC50 obtained after 12 h at 1.8 µg/ml by MTT assay. According to flow cytometric analysis, melittin induced apoptosis at concentrations more than 1 µg/ml.

These results suggest that melittin induces apoptotic cell death in cervical cancerous cells as observed by flow cytometric assay. It is concluded that melittin could be regarded as a potential candidate in future studies to discovery of new anticancer agents.

Thursday, October 18, 2018

Manuka Honey’s Anti-Viral, Anti-Bacterial, Anti-Inflammatory, and Antiseptic Properties


The Health Benefits of Manuka Honey

The Manual

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Manuka honey’s anti-viral, anti-bacterial, anti-inflammatory, and antiseptic properties are thought to be beyond comparison to other foods.

“All honey is said to have some activity level of positive benefits, but Manuka has been studied and sought after for it high non-peroxide and antibacterial activity,” says Blick. “It is dramatically more active than your average honey.”

Manuka is also used in adhesive dressings and wound gels, and as a part of DIY cleansing face masks.

“Honey has been used topically for centuries for its powerful antibacterial properties … (but) most of the average honey bear honey you see in the grocery stores lack the unique wellness compounds that are really rewarding for your skin,” says Comvita on their blog. “When it comes to beneficial properties, no honey compares to UMF Manuka Honey which contains guaranteed levels of leptosperin, methylglyoxal, and DHA.”

Yep, Manuka honey is so sought out that there have been counterfeit Manuka honey claims against faux Manuka.

And if all that sounds like mumbo jumbo, we’ll cut to the chase and tell you how to use Manuka honey this winter.

HOW TO USE MANUKA HONEY PROPERLY

By the Spoonful

Before heading to work, suck on a spoonful of 5+ Manuka honey. Done. Easy.

In Tea

While spritzers and ice-cold beers reign supreme during the summer, winter calls for a libation switch. Drink some tea, morning, noon, or night and add your Manuka Honey into the cup.

DIY Face Mask

Once or twice a week, do a Manuka face mask to rid dead skin and other impurities. You’ll want to use a high UMF-rated Manuka honey for your masks so mix 15+ or 10+ with raw sugar and a squeezed lemon (not so much that the mixture is runny). Scrub over your face and neck, leave for 20 mins, gently remove with warm water and a washcloth.

In or With Oatmeal, Chicken, or Ham

One of the easiest ways to get a Manuka boost is to mix the honey into everyday dishes. That can include being the sweetener for your steel cut oats, peanut butter banana toast, a glaze for your teriyaki chicken, or even a honey baked ham! Who said ham couldn’t be healthy?...

Wednesday, October 17, 2018

Main Source of Propolis Can Decrease Blood Glucose Levels and Insulin Resistance (Diabetes)


Beneficial Effects of Poplar Buds on Hyperglycemia, Dyslipidemia, Oxidative Stress, and Inflammation in Streptozotocin-Induced Type-2 Diabetes

J Immunol Res. 2018 Sep 18;2018:7245956

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The effects of propolis on blood glucose regulation and the alleviation of various complications caused by diabetes have been widely studied. The main source of propolis in the northern temperate zone is poplar buds. However, there is limited research on the antidiabetic activity of poplar buds.

In order to evaluate the effect of poplar buds on type-2 diabetes, crude extract and 50% fraction of poplar buds were used to feed streptozotocin-induced type-2 diabetic mice.

The results showed that 50% fraction could increase insulin sensitivity and reduce insulin resistance, as well as decrease the levels of fasting blood glucose, glycated hemoglobin, and glycosylated serum proteins in diabetic mice. Compared with the model control group, the 50% fraction-treated group showed significant decreases of malondialdehyde (MDA) and increases of superoxide dismutase (SOD) in serum and liver homogenate.

Moreover, 50% fraction could significantly decrease total cholesterol (TC), alleviate abnormal lipid metabolism, and enhance antioxidant capacity in the serum. For inflammatory factors, feeding of 50% fraction could also reduce the levels of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), monocyte chemotactic protein 1 (MCP-1), and cyclooxygenase-2 (COX-2) in liver homogenate.

Taken together, our results suggest that crude extract and 50% fraction of poplar buds, particularly the latter, can decrease blood glucose levels and insulin resistance, and 50% fraction can significantly relieve dyslipidemia, oxidative stress, and inflammation caused by type-2 diabetes.

Tuesday, October 16, 2018

Propolis Component May Help Treat Endometriosis


Chrysin leads to cell death in endometriosis by regulation of endoplasmic reticulum stress and cytosolic calcium level

J Cell Physiol. 2018 Oct 9

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Chrysin is a natural compound derived from honey, propolis, or passion flowers and has many functional roles, such as antiinflammatory and antiangiogenesis effects. Although endometriosis is a benign gynecological disease, there is a need to identify the pathology and develop a therapy for endometriosis. Elucidating the biological mechanism of chrysin on endometriosis will improve the understanding of endometriosis.

In this study, we confirmed the apoptotic effects of chrysin in human endometriotic cells using End1/E6E7 (endocervix-derived endometriotic cells) and VK2/E6E7 (vaginal mucosa-derived epithelial endometriotic cells).

The results showed that chrysin suppressed the proliferation of endometriosis and induced programmed cell death through changing the cell cycle proportion and increasing the cytosolic calcium level and generation of reactive oxygen species.

In addition, chrysin activated endoplasmic reticulum (ER) stress by stimulating the unfolded protein response proteins, especially the 78-kDa glucose-regulated protein-PRKR-like ER kinase (PERK)-eukaryotic translation initiation factor 2α (eIF2α) pathway in both endometriotic cell lines.

Furthermore, chrysin inactivated the intracellular phosphoinositide 3-kinase (PI3K)/protein kinase B (PKB, also known as AKT) signaling pathway in a dose-dependent manner. Collectively, the results of this study indicated that chrysin induced programmed cell death by activating the ER stress response and inactivating the PI3K signaling pathways in human endometriotic cells.

Monday, October 15, 2018

Honey Shows Antifungal Activity

Italian acacia honey exhibits lytic effects against the crayfish pathogens Aphanomyces astaci and Fusarium avenaceum

Lett Appl Microbiol. 2018 Oct 13

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This study purpose was to evaluate the in vitro inhibitory properties of Italian acacia honey extracts against pathogenic aquatic oomycete/fungal isolates that cause different diseases in crayfish, resulting in an elevated mortality rate.

The antimycotic activity of acacia honey aqueous extracts were evaluated against the strain UEF88662 of Aphanomyces astaci (oomycete) and the strain SMM2 of Fusarium avenaceum (fungus). The extracts preparation was carried out with water by a cheap, not complex and organic solvent-free procedure, with low environmental impact and the higher possibility of large-scale reproducibility. The anti-oomycete and antifungal activities were quantitatively evaluated by growth, survival and sporulation microbiological assays.

The extracts displayed a dose-dependent inhibitory efficacy on oomycete and fungal growth and survival, as well as on the production of oomycete and fungal spores. Supported by future in vivo studies, our results encourage the use of natural extracts like honey as innovative tools to counteract mycotic infections.

Saturday, October 13, 2018

Propolis Used to Treat Enlarged Prostate, Dandruff, Acne, Nail Fungus, Toothache, Ear Infections, Gastritis, Chronic Rhinitis

BEES-WORKERS TO NO END PRODUCE IT! FEW PEOPLE KNOW HOW TO TAKE…

October 13, 2018

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Бджоли-робітниці без кінця виробляють його! Мало хто знає, як правильно приймати… 

Propolis is a dark resinous substance produced by bees to glossing over cracks and isolation from foreign objects in the hive. Its other name is bee glue, in ancient times it was called the UGA, bee balm bee or plaster.

Bee glue has very strong antimicrobial properties. Thanks to the propolis the bees in the hive is always clean and pristine. Using propolis, bees mummified any insect or animal, such as a mouse, which they can’t get out of his nest, thus preventing rotting and decay.

THE PROPERTIES OF TINCTURE OF PROPOLIS

Due to its unique properties propolis is able to cope with many internal and external diseases. However, to achieve a positive effect, it must apply correctly. Dry propolis is used quite rarely, most often treatment is with the help of his tinctures.

In late February, I began to treat a sore throat, which worried me, no matter what medications I tried. A friend advised me to take tincture of propolis. I really was hoping for a quick result, but after a couple of days of bothersome pain in the throat disappeared.

Tincture of propolis once again proved its effectiveness, and I was curious to know what kind of healing properties it possesses. I already own the experience felt, how she copes with the cold, but how else can I use it?

THE USE OF TINCTURE OF PROPOLIS IN VARIOUS DISEASES

With stomatitis

Stomatitis hurts both children and adults. How to get rid of this disease without going to hospital? Really effective treatment — tincture of propolis.

Help with stomatitis rinse the mouth alcohol tincture of propolis. To start razbaby drug. In 1 Cup of warm liquid (milk, water) you need to add 15-20 grams of solution. Do rinse recommend about 1 month. Alcohol tincture is also used in the form of lotions.

Gastritis

Propolis for gastritis helps to relieve inflammation and pain. Thus it can be used with any acidity, including increased.Treatment of gastritis propolis involves strict control of the doctor and several different regimens, which ideally can be individualized after a complete diagnosis.

Chronic gastritis can be treated with 20% alcohol tincture. For this purpose it is necessary to take 40 drops three times per day. Drinking the extract of bee glue takes about two weeks. This assignment to do in normal and high acidity.

For toothache

Teeth and gums is generally a sore subject, and when they get sick, want to climb the wall. But if you have propolis, to relieve pain and make your mouth healthier can be a few procedures.Propolis not just temporarily relieves pain, eliminates unpleasant process, prevents the bacteria to continue the destructive influence to tissues of the tooth.

Rinse your mouth with tincture of propolis for tooth pain 3-4 times a day. So you will remove the inflammation of the gums, destroyed a fair amount of harmful bacteria that will get rid of the pain.

For otitis

For otitis media can be instilled 2 drops of the tincture in each ear to relieve inflammation. When purulent inflammation of the middle ear it is necessary to prepare the cords of the bandage, moisten the cloth in the tincture and insert in the ear for 20 minutes, pre gently clearing the ear pus.


In acute or chronic rhinitis

The mucosa of the nasopharynx — a hotbed of pathogenic microorganisms, which increase the symptoms of disease and weaken the immune system. To accelerate the treatment of chronic rhinitis, nasal wash salt solution with tincture of propolis.1 glass of water, take half a teaspoon of salt and half a teaspoon of tincture of propolis. Rinse nose best using special tools (available at pharmacies). You can use a small teapot infuser.

Got up the spout of the pot into your right nostril, tilt your head to the left, the water should be poured through the left nostril. Then do the same thing, washing the left side...

Friday, October 12, 2018

Bee Venom Helps Treat Atopic Dermatitis (Eczema)

Use of Bee Venom for Topical Treatment in Patients With Atopic Dermatitis

Dermatology Advisor

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The use of bee venom (BV) and its major component, melittin, has proven suitable as an epicutaneous application for the treatment of atopic dermatitis (AD), according to the results of an in vivo and in vitro study conducted in an AD-like model, with findings published in the British Journal of Pharmacology.

The investigators sought to explore the potential pharmacologic effects of bee venom and melittin on 1-chloro-2,4-dinitrobenzene (DNCB)-induced in vivo and tumor necrosis factor-alpha (TNF-α)/tumor necrosis factor-gamma (TNF-γ) in vitro AD-like models of skin disease.

Study results showed that bee venom and melittin possess potent anti-atopic activities, as demonstrated by decreased DNCB-induced AD-like skin lesions in an animal model. In vitro studies that used TNF-α/ TNF-γ–stimulated human keratinocytes revealed that bee venom and melittin inhibit the exaggerated expression of such chemokines as TARC/CCL17 and MDC/CCL22, as well as such pro-inflammatory cytokines as interleukin-1-beta (IL-1β), IL-6, and interferon (IFN)-γ, via blockade of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and signal transducers and activators of transcription (STAT) pathways.

The investigators concluded that since the use of topical agents is often recommended for the treatment of AD, bee venom and melittin are suitable for epicutaneous application. The results of this study showed the ameliorating effects of bee venom and melittin on the DNBC-induced AD-like skin lesions in BALB/c mice and TNF-α/ TNF-γ–stimulated human keratinocyte HaCaT cells.

By suppressing production of Th1/Th2–associated and pro-inflammatory cytokines, bee venom and melittin decreased inflammatory symptoms in AD-like skin lesions, resulting in a subsequent reduction in CD4+ T cells, mast cell infiltration, and release of serum immunoglobulin E (IgE). Moreover, bee venom and melittin improved abnormal epidermal differentiation by restoration of filaggrin expression.

'Mad Honey' Heals Infected Wounds

The effect of different types of honey on healing infected wounds

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J Wound Care. 2018 Oct 1;27(Sup10):S18-S25

OBJECTIVE:

To investigate the effects of treatments of 'mad honey', blossom honey and nitrofurazone on infected wound healing.

METHOD:

Male albino Wistar rats were randomly divided into four groups: 'mad honey' (MH), blossom honey (BH), nitrofurazone (N) and control (C). All rats were anaesthetised intraperitoneally. A circular skin incision was made to the back regions. Grafts containing slime-producing Staphylococcus epidermidis were placed on the incision area and then sutured to the skin. Infection in the wound area was confirmed after 48 hours. Wounds were dressed twice daily with the various treatment materials. Rats were randomly euthanised on days 7 or 14, and tissue samples taken. Tissue samples were assessed for hydroxyproline (HP), tensile strength (TS) and macroscopic measurement (area and intensity).

RESULTS:

HP levels were higher in the treatment groups (MH, BH, N) at days 7 and 14 compared with the control group. 'Group x day' interaction was found in the HP levels (p = 0.015). Increases in HP levels in the MH and N groups between days 7 and 14 were significantly higher than those in the other groups (p < 0.05). Intensity was significantly lower in the control group and significantly higher in group MH compared with the other groups. Significant 'group x day' interaction was observed in intensity (p = 0.006). TS was significantly lower on day 7 than on day 14 (p = 0.022). No marked difference was observed between the groups, nor any 'group x day' interaction, in terms of TS.

CONCLUSION:

Honey administration successfully healed infected wounds. However, there was no significant difference between the effect of MH and that of N in terms of wound healing.

Thursday, October 11, 2018

Propolis Accelerates Diabetic Wound Healing


Polysaccharide-based film loaded with vitamin C and propolis: A promising device to accelerate diabetic wound healing

Int J Pharm. 2018 Oct 6. pii: S0378-5173(18)30745-2

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Wound healing can be a painful and time-consuming process in patients with diabetes mellitus. In light of this, the use of wound healing devices could help to accelerate this process. Here, cellulose-based films loaded with vitamin C (VitC) and/or propolis (Prop), two natural compounds with attractive properties were engineered.

The starting materials and the cellulose-based films were characterized in detail. As assessed, vitamin C can be released from the Cel-PVA/VitC and Cel-PVA/VitC/Prop films in a controlled manner. In vitro antibacterial activity studies showed a reduction of bacteria counts (Escherichia coli and Staphylococcus aureus) after Cel-PVA/VitC, Cel-PVA/Prop, and Cel-PVA/VitC/Prop treatments. Moreover, we examined the antibacterial and wound healing properties of the cellulose-based films in a streptozotocin (STZ)-induced diabetic animal model. Diabetic mice exhibited impaired wound healing while the Cel-PVA/VitC/Prop treatment increased the wound closure.

A marked reduction in bacterial counts present in the wound environment of diabetic mice was observed after Cel-PVA/VitC, Cel-PVA/Prop and Cel-PVA/VitC/Prop treatment. Histological analysis demonstrated that the non-treated diabetic mice group did not exhibit adequate wound healing while the treated group with Cel-PVA/VitC and Cel-PVA/VitC/Prop films presented good cicatricial response. Furthermore, these novel eco-friendly films may represent a new therapeutic approach to accelerate diabetic wound healing.

Wednesday, October 10, 2018

6 Ways Manuka Honey Benefits Your Skin, Gut Health, and More (Probiotics, Acne, Wound Healing, Sinus, Diabetes)

Its potent antibacterial properties can treat cuts, calm acne, and bolster probiotics.

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By Maria Masters, Oct 9, 2018

Honey is one of those products that seems to do it all. It can sweeten any meal, hydrate parched skin, and soothe a sore throat when you’re feeling sick—but one particular honey stands out from the rest. Manuka honey, which is native to New Zealand and parts of Australia, has been gaining lots of buzz in the wellness world for its potent healing properties.

While some celebrities (like Kourtney Kardashian) are huge fans of the stuff, it’s also being researched by otolaryngologists, dermatologists, and nutritionists, many of whom are (at least cautiously) optimistic. Curious? Here’s everything you need to know about Manuka honey, its body-wide health benefits, and where to find it if you want to try it out for yourself.

Manuka is a special type of honey that’s made from bees that pollenate the Manuka plant, a shrub or small tree that’s native to New Zealand and eastern Australia. The Manuka flower produces a nectar that has unique compounds that aren’t as concentrated in other types of honey, including methylglyoxal, which is known for its antibacterial properties.

All genuine Manuka honey products have a Unique Manuka Factor (UMF) placed on the label, a trademark that rates the honey to signify the strength and quality of its signature properties, which is determined through independent testing. It’s these various compounds that have been linked to the health benefits of Manuka honey...

Tuesday, October 09, 2018

Bee Venom Can Kill HIV (AIDS)


Bee venom can kill virus, heals wounds faster

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By AbdulAzeez Bello

Dr Mkabwa Manoko of the University of Dar es Salaam, Tanzania, said bee venom could be used to kill deadly viruses in the body.

Dr. Manoko, also Head of the Department of Crop Sciences and Beekeeping Technology in the university, said this in an interview with the News Agency of Nigeria (NAN) on the sidelines of the just concluded ApiExpo Africa in Abuja.

He noted that honey also had a medicinal value that could be used for wound management without side effect.

The don said more studies and research implementation were required to tap into other potentialities of bees.

“Bees produce a lot of products that have industrial use in the pharmaceutical, food and beverage industries.

“Honey has medicinal property. It can be used for wound management, it is cheap, it takes a short time to heal and has no side effect like other chemicals.

“Bees produce propolis, venom, pollen, and all these are  valuable,” Manoko said.

He added that it was clearly established in research that bee venom had the ability to kill HIV, but not human cells. So it is something that research can explore to see how it can be used in the cure of HIV/AIDS....

Monday, October 08, 2018

Propolis Shows Anti-Inflammatory, Antibiotic, Antioxidative, Anticancer, Antifungal, Anesthetic, and Cytostatic Effects

3.3. Propolis

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Propolis or bee glue is a resinous substance that bees collect from various living plants for construction and adaptation of their nests, consisting of three major components: flavonoids, phenolic compounds, and caffeic acid phenethyl ester (CAPE). As a functional food, it has a range of biological activities such as anti-inflammatory, antibiotic, antioxidative, anticancer, antifungal, anesthetic, and cytostatic effects. In Caco-2 cells and in rats, propolis protects intestinal barrier function by increasing expression of tight junction loci occludin and zonula occluden and activating AMP-activated protein kinase and extracellular regulated protein kinase signaling. In intestinal epithelial cells, CAPE, as an active constituent of honeybee propolis, inhibits nuclear factor- (NF-) κB signaling and TNF-induced and IFN-induced protein- (IP-) 10 expression, independently of p38 mitogen-activated protein kinase (MAPK) and HO-1 signaling pathways, revealing its anti-inflammatory effect.

Sunday, October 07, 2018

Flavanones and Flavones from Bashkir Propolis


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Chemistry of Natural Compounds
September 2018, Volume 54, Issue 5, pp 975–977

Propolis is a product of the honey bee Apis mellifera and has a broad spectrum of biological activity (antimicrobial, antifungal, antitumor, antineurodegenerative, anti-inflammatory, antioxidant, cytotoxic, antituberculosis, antiviral, and immunomodulating) that is widely employed in traditional and folk medicine.

Greater than 500 compounds, of which more than 150 were flavonoids, were identified in the constituents of propolis depending on the geographic location, bee species, and plants.

The chemical composition of Bashkir propolis produced by A. mellifera from plants of the southern Urals (Bashkiria) was studied by us for the first time. Pure flavanones 1–4 and flavones 5 and 6 were isolated and identified.

Saturday, October 06, 2018

Propolis Component May Help Treat Brain Cancers Glioblastoma and Melanoma

Two Deadly Brain Cancers May Have Met Their Match

09/27/2018

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Glioblastoma and Melanoma metastasized to the brain are incredibly deadly brain cancers. However, pharmaceutical company Moleculin Biotech, Inc. hopes the diseases have met their match in a small molecule compound known as WP1066.

The company has initiated a Phase 1 clinical trial of the new first-in-class cancer drug candidate discovered by Professor Waldemar Priebe at The University of Texas MD Anderson Cancer Center. In animal models, WP1066 has shown to both inhibit an important cell signaling protein STAT3 that is involved in cell growth and proliferation, and also stimulate an immune response.

The first glioblastoma patient has received the initial doses of the compound at MD Anderson Cancer Center, and is said to have tolerated the dose well.

“Treating the first brain tumor patient with WP1066 is the start of a very exciting and encouraging program for doctors treating the worst types of brain cancers. There has been very little progress in recent years toward improved therapies for glioblastoma and other aggressive primary or metastatic brain tumors.  WP1066 has shown extremely promising results based on animal studies where we have seen inhibition of tumor growth and improvements in survival,” said Dr. Sandra Silberman, an oncologist and Moleculin’s CMO.

WP1066, which is built from the chemical backbone of the active ingredient in propolis, a natural product of honey bees, not only directly kills tumor cells, but also has the ability to overcome the tumor’s ability to evade the natural immune response, which would otherwise be working to eliminate the cancerous activity....

Friday, October 05, 2018

Propolis is a Natural Antibiotic

BEST NATURAL ANTIBIOTICS

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Antibiotics available in pharmacies should be used only in serious situations. Their uncontrolled use enhances the activity of viruses, fungi and bacteria. However, nature provides us with many antibiotics that are found in plants, roots and flowers. Excessive use of different types of synthetic antibiotics, offered through the pharmacological industry, eventually led to the fact that bacteria and viruses are becoming more resistant to chemicals used against them. Most of us are accustomed to use them in almost all cases, regardless of the scale of the problem. In the case of viral infections antibiotics don’t work.

Their excessive use of harmful bacterial intestinal flora. Therefore, synthetic antibiotics kill the good bacteria that leads to a significant reduction of resistance to all types of infections and diseases. Magicforum found out than you can replace these antibiotics.

Meanwhile, in nature there is a sufficiently large group of so-called natural antibiotics, which not only strengthen the immune system and help cure bacterial infections, but do that without compromising our health. Natural antibiotics contained in many foods and plants, and is an excellent alternative that allows us to take care of health.

Natural antibiotics...

6. Propolis

Propolis serves the bees to strengthen the hive, seals the nest and provides samesite from germs. In its composition it contains about 300 medicinal ingredients. Propolis has a strong antibacterial properties with a wide spectrum of action. It helps in the treatment of infections, gingivitis and periodontitis. It can also be used to treat eczema, boils, and diseases of the gastrointestinal tract. Ideal for strengthening the immune system.

Recipe for propolis tincture: 50 g propolis pour in a quarter of alcohol. Aged for 14 days in a tightly closed container in a cool, dark place, shaking occasionally, 2-3 times a day. Then remove the dark brown liquid from the sludge. Keep tincture in a dark glass bottle. Do not discard the sediment! You can use it to compress with the articular and rheumatic pains...

Thursday, October 04, 2018

Health Benefits of Bee Pollen Enhanced by Fermentation with Kombucha


Bee Collected Pollen with Enhanced Health Benefits, Produced by Fermentation with a Kombucha Consortium

Nutrients 2018, 10(10), 1365

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The bioavailability of pollen bioactive compounds for humans is limited. In this study, our aim was to enhance the health-related benefits of pollen by fermentation with a Kombucha/SCOBY (symbiotic culture of bacteria and yeasts) consortium.

We performed the fermentation of pollen suspended from the beginning with SCOBY on sweetened green tea or on Kombucha vinegar, by adding pollen after 20 days of Kombucha fermentation. We analyzed: formation of bioactive compounds (anti-oxidant polyphenols, soluble silicon, hydroxy-acids, short chain fatty acids—SCFA); parameters related to Kombucha fermentation (dynamics of lactic acid bacteria—LAB, formation of organic acids, soluble sugar evolution on Kombucha vinegar); the influence of Kombucha fermentation on pollen morphology and ultrastructure; in vitro cytotoxic and antitumoral effects of the Kombucha fermented pollen.

The pollen addition increases LAB proportion in the total number of SCOBY microbial strains. SEM images highlight the adhesion of the SCOBY bacteria to pollen. Ultrastructural analysis reveals the release of the pollen content. The content of bioactive compounds (polyphenols, soluble silicon species and SCFA) is higher in the fermented pollen and the product shows a moderate antitumoral effect on Caco-2 cells.

The health benefits of pollen are enhanced by fermentation with a Kombucha consortium.

Wednesday, October 03, 2018

Incorporation of Propolis is a Useful Strategy for Development of Active Packaging with Antioxidant and Antimicrobial Effects

Development of poly(lactic acid) films with propolis as a source of active compounds: Biodegradability, physical, and functional properties

Inc. J. Appl. Polym. Sci. 2018, 135, 47090

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Active films (AFs) using poly(lactic acid) (PLA) as a polymeric matrix containing various propolis concentrations (5, 8.5, and 13%) as the active agent (AA) were developed using a casting method.

The purpose was to determine the effects of the incorporation of AA on the physical properties of the films and to evaluate the antioxidant and antimicrobial activities. Tensile strength and elastic modulus of the AFs decreased relative to the control (PLA without AA). Introducing the active substances from propolis into the PLA also affected its thermal properties (glass transition).

Adding AAs to the polymer generated more opacity with a green‐yellowish color compared to the control. In addition, AFs exhibited reduced water vapor permeability as the AA concentration increased. Biodegradation assay showed that the AFs degraded faster than the control. AFs exhibited antioxidant activity, which was measured as the ability to scavenge free radicals (2,2‐diphenyl‐1‐picrylhydrazyl and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonate)), due to the presence of bioactive compounds (phenolics).

Antimicrobial activity was evaluated against Escherichia coli and showed a reduction over 4‐log cycles.

Therefore, incorporation of propolis is a useful strategy for the development of active packaging with antioxidant and antimicrobial effects, which increase the shelf life of food products.

Tuesday, October 02, 2018

Similarity of Data from Bee Bread with the Same Taxa Collected in India and Romania

Molecules 2018, 23(10), 2491

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Bee Bread samples from Romania and India were analysed by microscopy and High Performance Liquid Chromatography with Diode Array Detection (HPLC/DAD) and compared with pollen from the correspondent taxa.

The quantification of sugars, fructose/glucose ratio, total phenolics and flavonoids was also carried out. From the results was possible to identify Brassica and Eucalyptus samples that present similar HPLC/DAD profiles with the respective ultraviolet (UV) identification of the main compounds as Kaempferol-3-O-glycosides and Hydrocinnamic acid derivatives. The Fructose/Glucose (F/G) ratio and the total amounts of phenolics and flavonoids was in line with the prevalence of the specie identified.

These coincident fingerprints gave the identification of the samples, as was previously proposed for bee pollens. This paper relates for the first time the achievement on the taxon carried out previously only for bee pollens. It was reported for the first time that this phenolic profile remains unchanged in the case of floral pollen (hand collected), bee pollen and bee bread.

Despite the biochemical transformation that occurs during the fermentation of bee bread, it seems that these phenolic compounds are not affected and remain unchanged. Also, variables such as soil and climate do not seem to influence these compounds for the kind of samples under study.

Monday, October 01, 2018

Bee Venom Acupuncture May Help Treat Stroke, Depression, Parkinson's Disease, Lumbar Disc Disease, Osteoarthritis, Rheumatoid Arthritis, Adhesive Capsulitis, Lateral Epicondylitis

To bee or not to bee: The potential efficacy and safety of bee venom acupuncture in humans

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Toxicon. 2018 Sep 27. pii: S0041-0101(18)30393-3

Bee venom acupuncture is a form of acupuncture in which bee venom is applied to the tips of acupuncture needles, stingers are extracted from bees, or bees are held with an instrument exposing the stinger, and applied to acupoints on the skin.

Bee venom is a complex substance consisting of multiple anti-inflammatory compounds such as melittin, adolapin, apamin. Other substances such as phospholipase A2 can be anti-inflammatory in low concentrations and pro-inflammatory in others. However, bee venom also contains proinflammatory substances, melittin, mast cell degranulation peptide 401, and histamine. Nevertheless, in small studies, bee venom acupuncture has been used in man to successfully treat a number of musculoskeletal diseases such as lumbar disc disease, osteoarthritis of the knee, rheumatoid arthritis, adhesive capsulitis, and lateral epicondylitis.

Bee venom acupuncture can also alleviate neurological conditions, including peripheral neuropathies, stroke and Parkinson's Disease. The treatment has even been piloted in one series to alleviate depression. An important concern is the safety of bee venom. Bee venom can cause anaphylaxis, and several deaths have been reported in patients who successfully received the therapy prior to the adverse event. While the incidence of adverse events is unknown, the number of published reports of toxicity is small. Refining bee venom to remove harmful substances may potentially limit its toxicity. New uses for bee venom acupuncture may also be considered.

Sunday, September 30, 2018

Chinese Poplar Propolis Has Anti-Inflammatory Effects

Anti-inflammatory effects of Chinese propolis in lipopolysaccharide-stimulated human umbilical vein endothelial cells by suppressing autophagy and MAPK/NF-κB signaling pathway

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Inflammopharmacology. 2018 Sep 24

This study aimed to investigate the possible benefits of Chinese poplar propolis (CP) in inhibiting inflammation using vascular endothelial cells (VECs) cultured in a nutrient-rich condition exposed to lipopolysaccharide (LPS).

Cell proliferation was detected by sulforhodamine B assay and EdU kit. The production of reactive oxygen species (ROS) and level of mitochondrial membrane potential were determined with fluorescent probe DCHF and JC-1, respectively. Protein expression was examined by immunofluorescence staining and western blotting. The results showed that CP (6.25, 12.5, and 25 μg/mL) significantly reduced LPS-induced cytotoxicity, and when challenged with CP substantially suppressed ROS overproduction and protected mitochondrial membrane potential.

CP treatment significantly inhibited autophagy by inhibiting LC3B distribution and accumulation, and elevating the p62 level in an mTOR-independent manner but mainly by suppressing the translocation of p53 from the cytoplasm to the nucleus. Furthermore, CP treatment markedly reduced protein levels of TLR4 at 12 and 24 h and significantly suppressed nuclear translocation of NF-κB p65 from cytoplasm to nucleus. In addition, CP treatment significantly reduced the phosphorylation of JNK, ERK1/2, and p38 MAPK. Our findings demonstrated that CP protects VECs from LPS-induced oxidative stress and inflammation, which might be associated with depressing autophagy and MAPK/NF-κB signaling pathway.

The results provided novel insights for the potential use of nutrient-rich propolis against inflammation.

Saturday, September 29, 2018

Okinawan Propolis May Help Treat Type 2 Diabetes, Alzheimer's Disease, Cancer


Anti-Inflammatory, Anti-Diabetic, and Anti-Alzheimer's Effects of Prenylated Flavonoids from Okinawa Propolis: An Investigation by Experimental and Computational Studies

Molecules. 2018 Sep 27;23(10). pii: E2479\

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Okinawa propolis (OP) and its major ingredients were reported to have anti-cancer effects and lifespan-extending effects on Caenorhabditis elegans through inactivation of the oncogenic kinase, p21-activated kinase 1 (PAK1).

Herein, five prenylated flavonoids from OP, nymphaeol-A (NA), nymphaeol-B (NB), nymphaeol-C (NC), isonymphaeol-B (INB), and 3'-geranyl-naringenin (GN), were evaluated for their anti-inflammatory, anti-diabetic, and anti-Alzheimer's effects using in vitro techniques. They showed significant anti-inflammatory effects through inhibition of albumin denaturation (half maximal inhibitory concentration (IC50) values of 0.26⁻1.02 µM), nitrite accumulation (IC50 values of 2.4⁻7.0 µM), and cyclooxygenase-2 (COX-2) activity (IC50 values of 11.74⁻24.03 µM). They also strongly suppressed in vitro α-glucosidase enzyme activity with IC50 values of 3.77⁻5.66 µM. However, only INB and NA inhibited acetylcholinesterase significantly compared to the standard drug donepezil, with IC50 values of 7.23 and 7.77 µM, respectively.

Molecular docking results indicated that OP compounds have good binding affinity to the α-glucosidase and acetylcholinesterase proteins, making non-bonded interactions with their active residues and surrounding allosteric residues. In addition, none of the compounds violated Lipinski's rule of five and showed notable toxicity parameters. Density functional theory (DFT)-based global reactivity descriptors demonstrated their high reactive nature along with the kinetic stability.

In conclusion, this combined study suggests that OP components might be beneficial in the treatment of inflammation, type 2 diabetes mellitus, and Alzheimer's disease.

Friday, September 28, 2018

Manuka Honey Can Treat Recurrent Inflammation of Eyelids Caused by Mites (Ocular Demodex)

Comparing the in vitro effects of MGO™ Manuka honey and tea tree oil on ocular Demodex viability

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Cont Lens Anterior Eye. 2018 Jul 6. pii: S1367-0484(18)30776-8

PURPOSE:

To compare the in vitro antiparasitic effects of MGO™ Manuka honey and tea tree oil against ocular Demodex.

METHODS:

Fifty-two viable Demodex mites were acquired from the epilated eyelashes of 9 participants with blepharitis and symptomatic dry eye. Viable mites were randomised to one of five treatment groups: cyclodextrin-complexed and uncomplexed Manuka Honey, 100% and 50% tea tree oil, and no treatment. Following treatment application, mite viability was assessed for 240 min, based on limb and body movement and/or the development of a crenated/translucent appearance. Kaplan-Meier survival analysis was then performed.

RESULTS:

The log-rank test demonstrated a significant treatment effect on the survival distribution of Demodex mites (p < 0.001). Bonferroni-corrected post-hoc pairwise analysis showed that all treatments except for uncomplexed honey effected lower survival probabilities than the untreated group (all p < 0.001). Among the four treatments, survival probabilities were lowest with 100% tea tree oil (all p < 0.001), and highest with uncomplexed honey (all p ≤ 0.001). No difference was observed between complexed honey and 50% tea tree oil (p = 0.81).

CONCLUSIONS:

The in vitro efficacy of cyclodextrin-complexed Manuka honey was comparable with 50% tea tree oil, an established treatment for ocular Demodex. The findings support future clinical trials investigating the therapeutic effects of complexed honey in demodectic blepharitis patients.

Thursday, September 27, 2018

Nigeria: Bees’ Venom Can Kill Virus, Heal Wounds Faster (HIV/AIDS, Beekeeping)

Published September 26, 2018

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Dr Mkabwa Manoko of the University of Dar es Salaam, Tanzania, on Wednesday said that bees’ venom could be used to kill deadly viruses in the body.

Manoko, also Head of Department of Crop Sciences and Bee-Keeping Technology at the university, expressed the viewpoint in an interview with the News Agency of Nigeria on the sidelines of the 6th ApiExpo Africa in Abuja.

He noted that honey also had a medicinal value that could be used for wound management without side effects.

The university lecturer said that more studies and research implementation were required to tap into other potentials of bees.

“Bees produce a lot of products that have industrial use in the pharmaceutical, food and beverage industries.

“Honey has medicinal property; it can be used for wound management, it is cheap, it takes short time to heal, has no side effect like other chemicals.

“Bees produce propolis, venom, pollen and all these are all valuable,” Manoko said.

According to the lecturer, it is clearly established in research that bee venom has the ability to kill HIV, but not the human cells; so, it is something that research can explore to see how it can be used in the cure of HIV/AIDS.

“More research and the application of the researches on bee-keeping are needed. We normally carry out research, but we do not develop them,’’ he said.

Wednesday, September 26, 2018

Propolis Components May Help Treat Breast Cancer (Epithelium Mammary Carcinoma)

Caffeic Acid Versus Caffeic Acid Phenethyl Ester in the Treatment of Breast Cancer MCF-7 Cells: Migration Rate Inhibition

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Integr Cancer Ther. 2018 Sep 24:1534735418801521

Epithelium mammary carcinoma is a cancer with a high death rate among women. One factor having a significant impact on metastasis is cell migration. The aim of this study was to compare migration rate inhibition of caffeic acid (CA) and its phenethyl ester (CAPE) on MCF-7 breast cancer cells.

Microscopic evaluation was used to determine the morphology of carcinoma cells, before and after 24-hour treatment with CA and CAPE using a dose of 50 µM. The cytotoxic effect was measured by XTT-NR-SRB assay (tetrazolium hydroxide-neutral red-Sulforhodamine B) for 24-hour and 48-hour periods, using CA and CAPE, with doses of 50 and 100 µM. These doses were used to determine cell migration inhibition using a wound closure assay for 0-hour, 8-hour, 16-hour, and 24-hour periods.

Both CA and CAPE treatments displayed cytotoxic activity in a dose- and time-dependent trend. CAPE displayed IC50 values more than twice as low as CA. IC50 values for the XTT assay were as follows: CA was 102.98 µM for 24 hours and 59.12 µM for 48 hours, while CAPE was 56.39 µM for 24 hours and 28.10 µM for 48 hours. For the NR assay: CA was 84.87 µM at 24 hours and 65.05 µM at 48 hours, while CAPE was 69.05 µM at 24 hours and 29.05 µM at 48 hours. For the SRB assay: At 24 hours, CA was 83.47 µM and 53.46 µM at 48 hours, while CAPE was 38.53 µM at 24 hours and 20.15 µM at 48 hours.

Both polyphenols induced migration inhibition, resulting in practically halting the wound closure. CAPE produced better results than CA with the same doses and experiment times, though both CA and CAPE displayed cytotoxic activity against MCF-7 cells, as well as inhibited migration.

Tuesday, September 25, 2018

Aloe Vera, Honey Wound Dressing Promotes Scar-Free Healing

Scar free healing mediated by the release of aloe vera and manuka honey from dextran bionanocomposite wound dressings

Int J Biol Macromol. 2018 Sep 21

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Scar preventive dextran based bionanocomposite dressings containing aloe vera (AV) and manuka honey (MH) were developed as wound care devices. This work was a challenge to fabricate herbal dressing that promotes healing, which at the same time is biocompatible, non-toxic, biodegradable, and cost effective in terms of the simplicity of application in complex chronic wound situations.

With this aim, we synthesized in-situ crosslinked dextran/nanosoy/glycerol/chitosan (DNG/Ch) nanocomposite membranes via solvent casting technique followed by subsequent addition of AV and MH to obtain DNG/Ch/AV and DNG/Ch/MH herbal dressings.

The drug release kinetics of the bionanocomposite dressings indicated an initial burst release of AV and MH, followed by controlled release when examined in-vitro using non-fickian and quasi-fickian model.

Antibacterial studies confirmed >99% antibacterial activity against both Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) and bacterial adherence test demonstrated its efficacy for arresting microbial invasion.

Wound healing analysis conducted in-vivo showed enhanced epithelialization in the terms scar prevention and aesthetics with absolute wound contraction for the mice treated with AV loaded dressings after 14th post wounding day. Histological features displayed ordered deposition of collagen with a thin epidermis layer.

Hence, the present herbal dressing could function without many cytotoxicity and biocompatibility issues.

Monday, September 24, 2018

Propolis May Help Prevent Colitis

Propolis from Different Geographic Origins Decreases Intestinal Inflammation and Bacteroides spp. Populations in a Model of DSS‐Induced Colitis 

Molecular Nutrition & Food Research

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1 Scope

Dietary supplementation with polyphenol‐rich propolis can protect against experimentally induced colitis. We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)‐induced colitis in rats.

2 Methods and results

HPLC‐DAD/Q‐TOF‐MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar. Rats were given CP or BP by gavage (300 mg kg−1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS. CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS‐induced colonic tissue damage, and increased resistance to DSS‐induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T‐AOC levels. CP and BP significantly reduced DSS‐induced colonic apoptosis. Colonic inflammatory markers IL‐1β, IL‐6, and MCP‐1 were suppressed by CP and BP, whereas only BP‐induced expression of TGF‐β. CP, not BP, increased the diversity and richness of gut microbiota populations. Both forms of propolis significantly reduced populations of Bacteroides spp.

3 Conclusions

Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS‐induced colitis is similar. Nevertheless, some different physiological impacts were observed.

Sunday, September 23, 2018

Propolis Helps Treat Hyperglycemia (High Blood Sugar), Dyslipidemia (High Cholesterol), Oxidative Stress, and Type-2 Diabetes

Beneficial Effects of Poplar Buds on Hyperglycemia, Dyslipidemia, Oxidative Stress, and Inflammation in Streptozotocin-Induced Type-2 Diabetes

Journal of Immunology Research
Volume 2018, Article ID 7245956, 10 pages

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The effects of propolis on blood glucose regulation and the alleviation of various complications caused by diabetes have been widely studied. The main source of propolis in the northern temperate zone is poplar buds. However, there is limited research on the antidiabetic activity of poplar buds.

In order to evaluate the effect of poplar buds on type-2 diabetes, crude extract and 50% fraction of poplar buds were used to feed streptozotocin-induced type-2 diabetic mice. The results showed that 50% fraction could increase insulin sensitivity and reduce insulin resistance, as well as decrease the levels of fasting blood glucose, glycated hemoglobin, and glycosylated serum proteins in diabetic mice. Compared with the model control group, the 50% fraction-treated group showed significant decreases of malondialdehyde (MDA) and increases of superoxide dismutase (SOD) in serum and liver homogenate. Moreover, 50% fraction could significantly decrease total cholesterol (TC), alleviate abnormal lipid metabolism, and enhance antioxidant capacity in the serum. For inflammatory factors, feeding of 50% fraction could also reduce the levels of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), monocyte chemotactic protein 1 (MCP-1), and cyclooxygenase-2 (COX-2) in liver homogenate.

Taken together, our results suggest that crude extract and 50% fraction of poplar buds, particularly the latter, can decrease blood glucose levels and insulin resistance, and 50% fraction can significantly relieve dyslipidemia, oxidative stress, and inflammation caused by type-2 diabetes.

Saturday, September 22, 2018

Beeswax By-Products Protect Cells That Promote Skin Healing

Beeswax by-Products Efficiently Counteract the Oxidative Damage Induced by an Oxidant Agent in Human Dermal Fibroblasts

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Int J Mol Sci. 2018 Sep 19;19(9). pii: E2842

The antioxidant capacity and the phytochemical composition of two by-products from beeswax recycling processes were recently investigated.

The aim of the present work was to evaluate the efficacy of one of these by-products, MUD1, against the oxidative stress induced by 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) in human dermal fibroblast (HDF) cells. After a preliminary viability assay, the protective effect of MUD1 was investigated through the measurement of apoptosis level, the reactive oxygen species (ROS) and nitrite (NO₂-) production, the level of protein and lipid biomarkers (carbonyl groups, total glutathione and thiobarbituric acid-reactive substance) of oxidative damage, and the measurement of antioxidant enzymes activities (glutatione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase and catalase).

The obtained results showed that MUD1 exerted protective effects on HDF, increasing cell viability and counteracted the oxidative stress promoted by AAPH-treatment, and improved mitochondria functionality and wound healing capacities.

This work shows the antioxidant effects exerted by beeswax by-products, demonstrating for the first time their potential against oxidative stress in human dermal fibroblast cells; however, further research will be necessary to evaluate their potentiality for human health by more deeply in vitro and in vivo studies.

Friday, September 21, 2018

Honey Boosts Acyclovir in Treating Herpes Simplex Gingivostomatitis (HSGS)

Honey can help in herpes simplex gingivostomatitis in children: Prospective randomized double blind placebo controlled clinical trial

Am J Otolaryngol. 2018 Sep 12. pii: S0196-0709(18)30581-7

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PURPOSE:

Herpes simplex gingivostomatitis (HSGS) in children is a common painful infectious disease. This study aims to examine the combined efficacy of honey with acyclovir suspension compared to acyclovir alone for treating HSGS in young children.

MATERIAL AND METHODS:

This Randomized double blind placebo controlled study was conducted from June 2015 to September 2017 in a tertiary referral hospital. One hundred children aged 2-8 years with HSGS were randomly classified into 2 groups; study group: treated with honey plus oral acyclovir and control group: treated with oral acyclovir alone. Severity of oral lesions, Fever, eating and drinking ability, pain scores and need for analgesics were compared between 2 groups on day 3, 5 and 7 after starting treatment.

RESULTS:

Children receiving honey plus acyclovir (i.e. study group) had significantly earlier disappearance of herpetic oral lesions; median 3 days vs. 6 days in control group (P = 0.022), drooling; 2 days vs. 4 days (P = 0.030) and eating difficulty; 3 days vs. 8 days (P = 0.001). Study group also had significantly lower pain scores, better eating and drinking ability and significantly less need for analgesics at 3 time-points of assessment. Fever disappeared in both groups with no statistically significant difference.

CONCLUSIONS:

The combined use of honey with oral acyclovir can produce favorable outcome than acyclovir alone in children with Primary herpetic gingivostomatitis.

Thursday, September 20, 2018

Propolis Helps Treat Gingivitis

Propolis as an adjuvant to non-surgical periodontal treatment: a clinical study with salivary anti-oxidant capacity assessment

Minerva Stomatol. 2018 Oct;67(5):183-188

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BACKGROUND:

Periodontal diseases are characteristic for the excessive release of oxidant free-radicals by the host. The aim of the present study was to evaluate the efficacy of an anti-oxidant-based formula containing propolis and herbs as an adjunctive therapy to standard non-surgical periodontal treatment (NSPT) when compared to the domiciliary use of chlorhexidine-based formulae.

METHODS:

Forty patients were enrolled in the present study and randomly allocated to either a control (NSPT plus chlorhexidine gel formula) group or a test (NSPT plus anti-oxidant gel formula) group. Clinical parameters for the assessment of the periodontal status were evaluated at baseline, one month, and three months after NSPT, and the salivary antioxidant capacity as well.

RESULTS:

There were no significant clinical differences between the two groups (P > 0.05). However, patients within the test group (propolis) achieved better results in terms of oxidative stress reduction (P < 0.05).

CONCLUSIONS:

In the present study, propolis was comparable to chlorhexidine in the clinical management of gingivitis. Further studies are needed to investigate its potential as a redox modulator for the oral microbiome.

Wednesday, September 19, 2018

Everything You Need to Know About Health Benefits of Manuka Honey

DailyAddaa

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Why Manuka honey?

Manuka honey is the natural ointment for healing wounds of all kinds. It often addressed as a germ fighter in the age of antibiotics. This lesser used traditional remedy that also benefits in acne and sinus issues.

Manuka honey is produced in New Zealand by bees that pollinate the native manuka bush. When bees pollinate from scrub plant, their honey is more potent because of higher concentration of methylglyoxal (MGO).

What are the advantages of manuka honey?

Raw honey is associated with health benefits whereas manuka is specialised in antibacterial and bacterial resistant.

1. Accountable for treating both acute and chronic diseases.
2. Helps in healing scrapes
3. Promotes oral health
4. Soothes a soar throat
5. Prevention from gastric ulcers
6. Improves digestion system
7. Treats acne
8. Clearing infections
9. Boosting the immune system
10. Provides energy

Tuesday, September 18, 2018

Bee Venom Helps Treat Rheumatoid Arthritis


Bee Venom and Hesperidin Effectively Mitigate Complete Freund's Adjuvant-Induced Arthritis Via Immunomodulation and Enhancement of Antioxidant Defense System

Arch Rheumatol. 2017 Nov 2;33(2):198-212

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Objectives:

This study aims to assess the antirheumatic activity of bee venom (BV) and/or hesperidin as natural products in complete Freund's adjuvant (CFA)-induced arthritis in male Wistar rats.

Material and methods:

Rheumatoid arthritis was induced in 30 male Wistar rats (weight 130 g to 150 g; age 10 to 12 weeks) by subcutaneous injection of CFA into the right hind paw of the rats. The rats were divided into five groups of six rats in each and administered the following regimens for 21 days: Normal group (given the equivalent volume of saline and carboxymethylcellulose), arthritic group (given the equivalent volume of saline and carboxymethylcellulose), arthritic group treated with BV (treated with BV along with carboxymethylcellulose), arthritic group treated with hesperidin (treated with hesperidin along with saline), and arthritic group treated with BV and hesperidin (treated with BV and hesperidin concurrently).

Results:

Bee venom and/or hesperidin successfully reversed the CFA-arthritis-induced increases in right hind leg paw swelling, leukocytes' count, liver lipid peroxidation, serum inflammatory cytokine interleukin (IL-2 and IL-12) levels and spleen tumor necrosis factor-alpha messenger ribonucleic acid expression. Moreover, the CFA-induced down-regulation in serum IL-10 level and spleen IL-4 messenger ribonucleic acid expression as well as the deterioration in the antioxidant defense system were significantly improved as a result of BV and hesperidin administration. Both treatments also markedly counteracted the severe inflammatory changes and leukocytic infiltration in the periarticular tissue of the ankle joints. In addition, BV and hesperidin obviously amended the lymphoid hyperplasia in white pulps of spleen as well as the widening of the medulla and mononuclear cell infiltration found in thymus.

Conclusion:

Bee venom and hesperidin administration produced their ameliorative effects on rheumatoid arthritis via their antioxidant, antiinflammatory and immunomodulatory potentials. BV plus hesperidin particularly seemed to be the most potent in improving rheumatoid arthritis in Wistar rats.

Monday, September 17, 2018

Propolis May Help Treat High Cholesterol

Lipid-lowering effect of propolis in mice with Triton-WR1339-induced hyperlipidemia and its mechanism for regulating lipid metabolism [Article in Chinese]

Nan Fang Yi Ke Da Xue Xue Bao. 2018 Jul 30;38(8):1020-1024

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OBJECTIVE:

To evaluate the therapeutic effect of propolis against Triton-WR1339-induced hyperlipidemia in mice and explore the underlying mechanism.

METHODS:

C57BL/6 mice were randomly divided into 7 groups (n=10), including the control group, hyperlipidemia model group, fenofibrate (30 mg/kg) treatment group, and 4 treatment groups treated with low- (30 mg/kg) or high-dose (60 mg/kg) propolis HB01 or HB02. In all but the control group, acute hyperlipidemia models were established by intramuscular injection of Triton WR-1339, and corresponding treatments were administered via gastric lavage for 7 days. After the treatments, blood samples were collected for testing the levels of total cholesterol (TC), triglycerides (TG), highdensity lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), malondialdehyde (MDA), superoxide dismutase (SOD), alanine aminotransferase (GPT), and aspartate aminotransferase (GOT); Western blotting was used to detect the expressions of the proteins involved in lipid metabolism in the liver tissues including ABCA1, ABCG8, LDLR, and SR-B1.

RESULTS:

Compared with the normal control group, the mice with Triton-WR1339-induced hyperlipidemia showed significantly increased levels of TC, TG, LDL, MDA, GPT, and GOT and lowered HDL-C levels and SOD activity (P < 0.05). Treatments with fenofibrate and the 2 propolis at either low or high dose significantly reversed Triton-WR1339-induced changes in blood lipids (P < 0.05), and the effects of propolis were more potent. Triton-WR1339 injection also significantly decreased the expressions levels of ABCA1, ABCG8, LDLR, and SR-B1 in the liver (P < 0.05), and these changes were obviously reversed by treatments with fenofibrate and propolis (P < 0.05), especially by the latter.

CONCLUSIONS:

The lipid-lowering effects of propolis are mediated by improving lipid metabolism and regulating the expressions of lipid transport proteins in the liver tissue.

Sunday, September 16, 2018

Probiotic Honey Has Beneficial Effects on Insulin Metabolism (Diabetes, Diabetic Nephropathy), Total-/HDL-Cholesterol, Serum Hs-CRP, And Plasma MDA Levels

The Effects of Probiotic Honey Consumption on Metabolic Status in Patients with Diabetic Nephropathy: a Randomized, Double-Blind, Controlled Trial.

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Probiotics Antimicrob Proteins. 2018 Sep 14

To the best of our knowledge, this study is the first evaluating the effects of probiotic honey intake on glycemic control, lipid profiles, biomarkers of inflammation, and oxidative stress in patients with diabetic nephropathy (DN).

This investigation was conducted to evaluate the effects of probiotic honey intake on metabolic status in patients with DN. This randomized, double-blind, controlled clinical trial was performed among 60 patients with DN.

Patients were randomly allocated into two groups to receive either 25 g/day probiotic honey containing a viable and heat-resistant probiotic Bacillus coagulans T11 (IBRC-M10791) (108 CFU/g) or 25 g/day control honey (n = 30 each group) for 12 weeks. Fasting blood samples were taken at baseline and 12 weeks after supplementation to quantify glycemic status, lipid concentrations, biomarkers of inflammation, and oxidative stress.

After 12 weeks of intervention, patients who received probiotic honey compared with the control honey had significantly decreased serum insulin levels (- 1.2 ± 1.8 vs. - 0.1 ± 1.3 μIU/mL, P = 0.004) and homeostasis model of assessment-estimated insulin resistance (- 0.5 ± 0.6 vs. 0.003 ± 0.4, P = 0.002) and significantly improved quantitative insulin sensitivity check index (+ 0.005 ± 0.009 vs. - 0.0007 ± 0.005, P = 0.004).

Additionally, compared with the control honey, probiotic honey intake has resulted in a significant reduction in total-/HDL-cholesterol (- 0.2 ± 0.5 vs. + 0.1 ± 0.1, P = 0.04). Probiotic honey intake significantly reduced serum high-sensitivity C-reactive protein (hs-CRP) (- 1.9 ± 2.4 vs. - 0.2 ± 2.7 mg/L, P = 0.01) and plasma malondialdehyde (MDA) levels (- 0.1 ± 0.6 vs. + 0.6 ± 1.0 μmol/L, P = 0.002) compared with the control honey. Probiotic honey intake had no significant effects on other metabolic profiles compared with the control honey.

Overall, findings from the current study demonstrated that probiotic honey consumption for 12 weeks among DN patients had beneficial effects on insulin metabolism, total-/HDL-cholesterol, serum hs-CRP, and plasma MDA levels, but did not affect other metabolic profiles.

Friday, September 14, 2018

Royal Jelly, Brazilian Green Propolis May Help Treat Allergic Rhinitis (Allergies, Itchy, Watering Eyes, Sneezing)

Effect of Royal Jelly and Brazilian Green Propolis on the Signaling for Histamine H1 Receptor and Interleukin-9 Gene Expressions Responsible for the Pathogenesis of the Allergic Rhinitis

Biol Pharm Bull. 2018;41(9):1440-1447

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The significant correlation between nasal symptom scores and level of histamine H1 receptor (H1R) mRNA in nasal mucosa was observed in patients with pollinosis, suggesting that H1R gene is an allergic disease sensitive gene. We demonstrated that H1R and interleukin (IL)-9 gene are the allergic rhinitis (AR)-sensitive genes and protein kinase Cδ (PKCδ) signaling and nuclear factor of activated T-cells (NFAT) signaling are involved in their expressions, respectively. Honey bee products have been used to treat allergic diseases. However, their pathological mechanism remains to be elucidated. In the present study, we investigated the mechanism of the anti-allergic effect of royal jelly (RJ) and Brazilian green propolis (BGPP). Treatment with RJ and BGPP decreased in the number of sneezing on toluene 2,4-diissocyanate (TDI)-stimulated rats. The remarkable suppression of H1R mRNA in nasal mucosa was observed. RJ and BGPP also suppressed the expression of IL-9 gene. RJ and BGPP suppressed phorbol-12-myristate-13-acetate-induced Tyr311 phosphorylation of PKCδ in HeLa cells. In RBL-2H3 cells, RJ and BGPP also suppressed NFAT-mediated IL-9 gene expression. These results suggest that RJ and BGPP improve allergic symptoms by suppressing PKCδ and NFAT signaling pathways, two important signal pathways for the AR pathogenesis, and suggest that RJ and BGPP could be good therapeutics against AR.

Thursday, September 13, 2018

Honey Mouthwash Helps Treat Mucositis Caused by Acute Myeloid Leukemia

The effect of an oral care protocol and honey mouthwash on mucositis in acute myeloid leukemia patients undergoing chemotherapy: a single-blind clinical trial

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Clin Oral Investig. 2018 Sep 11

OBJECTIVES:

The purpose of the study is to evaluate and compare the effectiveness of honey mouthwash and an oral care protocol on mucositis and weight loss in patients with acute myeloid leukemia receiving chemotherapy.

MATERIALS AND METHODS:

In this single-blind clinical trial, 53 acute myeloid leukemia (AML) patients receiving chemotherapy were randomly assigned into three groups: honey mouthwash (n = 17), oral care (n = 17), and control (n = 19). The severity of mucositis and weights was examined blindly at the baseline and 4-week follow-up.

RESULTS:

The prevalence of grades of mucositis in the study groups was significant at the end of the third (p = 0.002) and fourth (p < 0.001) weeks. The mucositis severity decreased at the end of the third and fourth weeks in the honey mouthwash group (p < 0.05), whereas it increased in the control group (p < 0.001). The difference in the weight was significant between the honey mouthwash and the control groups (p < 0.05, MD = 1.95) at the end of the third week, and between the honey mouthwash group with the control (p < 0.01, MD = 2.92) and oral care groups (p < 0.05, MD = 1.95) at the end of the fourth week.

CONCLUSIONS:

Honey mouthwash is effective in preventing and reducing the severity of mucositis, and weight loss and can be recommended for patients undergoing chemotherapy.

CLINICAL RELEVANCE:

The results of this study suggest that honey mouthwash can reduce the incidence and severity of mucositis in patients, reduce or eliminate the possibility of weight loss in them, as well as encourage some weight gain. Compared to routine oral care, honey mouthwash is also easier to use and handle.

Wednesday, September 12, 2018

Propolis May Help Treat Colitis


Propolis from Different Geographic Origins Decreases Intestinal Inflammation and Bacteroides spp. Populations in a Model of DSS‐Induced Colitis 

Molecular Nutrition, Volume62, Issue17
September 2018

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1 Scope

Dietary supplementation with polyphenol‐rich propolis can protect against experimentally induced colitis. We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)‐induced colitis in rats.

2 Methods and results

HPLC‐DAD/Q‐TOF‐MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar. Rats were given CP or BP by gavage (300 mg kg−1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS. CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS‐induced colonic tissue damage, and increased resistance to DSS‐induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T‐AOC levels. CP and BP significantly reduced DSS‐induced colonic apoptosis. Colonic inflammatory markers IL‐1β, IL‐6, and MCP‐1 were suppressed by CP and BP, whereas only BP‐induced expression of TGF‐β. CP, not BP, increased the diversity and richness of gut microbiota populations. Both forms of propolis significantly reduced populations of Bacteroides spp.

3 Conclusions

Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS‐induced colitis is similar. Nevertheless, some different physiological impacts were observed.

Tuesday, September 11, 2018

Bee Venom May Help Treat Cancer, Tuberculosis (TB), HIV (Human Immunodeficiency Virus)

Venom as therapeutic weapon to combat dreadful diseases of 21st century: A systematic review on cancer, TB, and HIV/AIDS

Microb Pathog. 2018 Sep 6. pii: S0882-4010(18)31390-1

BUY Concentrated Propolis in Veggie Capsules  

Cancer and infectious diseases are the preeminent causes of human morbidities and mortalities worldwide. At present, chemotherapy, radiotherapy, immunotherapy, and gene therapy are considered as predominant options in order to treat cancer. But these therapies provide inadequate consequences by affecting both the normal and tumor cells. On the other hand, tuberculosis (TB), and HIV (human immunodeficiency virus) infections are significant threats, causing over a million mortalities each year.

The extensive applications of antibiotics have caused the microbes to acquire resistance to the existing antibiotics. With the emerging dilemma of drug resistant microbes, it has become imperative to identify novel therapeutic agents from natural sources as emphatic alternative approach. Over the past few decades, venoms derived from several reptiles, amphibians, and arthropods including snakes, scorpions, frogs, spiders, honey bees, wasps, beetles, caterpillars, ants, centipedes, sponges etc. have been identified as efficient therapeutics. Venoms constitute plethora of bioactive components, particularly peptides, enzymes, and other chemical entities, which exhibit a large array of anticancer and anti-pathogenic activities.

This review highlights the panorama of bioactive components of animal venoms divulging the anticancer, anti-tubercular, and anti-HIV activities. In a nutshell, this context discloses the decisive role of animal venoms as alternative natural resources to combat these deadly diseases of 21st century, and propounding the plausible development of new therapeutic drugs in the present era.

Monday, September 10, 2018

Analysis of Manuka Honey’s Antioxidant, Antimicrobial and Wound Healing Capacities

Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages. Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality

Food Chem Toxicol. 2018 Sep 3;121:203-213

BUY Concentrated Propolis in Veggie Capsules  

Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities.

The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved.

Up to 18 compounds were identified in MH, with gallic acid and quercetin as the major ones; MH showed also remarkable TAC. In addition, MH was able to enhance cellular viability, decrease apoptosis, promote wound healing and attenuate inflammation in a dose-dependent manner, by reducing the expression of caspase 3, p-p38 and p-Erk1/2 proteins, in macrophages stressed with LPS. In addition, it improved mitochondrial respiration and glycolytic activities, stimulating the expression of p-AMPK, SIRT1 and PGC1α, counteracting in this way the deleterious effects of LPS treatment.

In conclusion, one of the possible mechanisms by which MH exerts its beneficial effects could be to its capacity to improve cellular viability, promote proliferation and enhance energetic metabolism, by modulating the expression of several proteins involved in apoptosis, inflammation, metabolism and mitochondrial biogenesis.

Saturday, September 08, 2018

Bee Venom May Help Treat Eczema

EurekAlert

BUY Concentrated Propolis in Veggie Capsules  

Bee venom and its major component, melittin, may be effective treatments for atopic dermatitis (or eczema), according to a British Journal of Pharmacology study.

Through studies conducted in mice and in human cells, investigators found that bee venom and melittin suppress inflammation through various mechanisms on immune cells and inflammatory molecules.

"This study demonstrated that bee venom and melittin have immunomodulatory activity, and such activity was associated with the regulation of T helper cell differentiation, thereby ameliorating the inflammatory skin diseases caused by atopic dermatitis," the authors wrote.