Friday, May 24, 2019

Bee Venom Component May Help Treat Muscle Injuries


Melittin - A bee venom component - Enhances muscle regeneration factors expression in a mouse model of skeletal muscle contusion

J Pharmacol Sci. 2019 May 4. pii: S1347-8613(19)31050-3

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Melittin is a major peptide component of sweet bee venom that possesses anti-allergic, anti-inflammatory, anti-arthritis, anti-cancer, and neuroprotective properties. However, the therapeutic effects of melittin on muscle injury have not been elucidated.

We investigated the therapeutic effects of melittin on muscle injury in a mouse model of muscle contusion. The biceps femoris muscle of the mice was injured using drop mass method, and the animals were treated with melittin (4, 20, or 100 μg/kg) for 7 days. Melittin significantly increased: locomotor activity in open field test, and treadmill running activity in a dose-dependent manner to level comparable to the positive control, diclofenac (30 mg/kg). Melittin treatment attenuated the pro-inflammatory cytokine MCP-1, TNF-α and IL-6.

The expression of muscle regeneration biomarkers, including MyoD (muscle differentiation marker), myogenin, smooth muscle actin, and myosin heavy chain was markedly increased in the injured muscle tissue of melittin-treated mice, as determined by western blotting and quantitative real-time polymerase chain reaction.

These results demonstrate that melittin inhibits inflammatory response and improves muscle damage by regenerating muscles in a mouse model of muscle contusion. Taken together, the results of present study suggest that melittin is a promising candidate for the muscle injury treatment.

Wednesday, May 22, 2019

Propolis May Help Prevent Cancer in Smokers


Detoxification effects of aloe polysaccharide and propolis on the urinary excretion of metabolites in smokers

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Food Chem Toxicol. 2019 May 18. pii: S0278-6915(19)30296-0

The aim of the present study was to investigate the detoxifying effects of aloe polysaccharide (APS), propolis, and the mixture of APS and propolis on the urinary excretion of major human tobacco carcinogens, BaP and an addictive stimulant alkaloid, nicotine. Smokers (≥20 cigarettes/day) were randomly classified into four subgroups (10 people/group) and were given 600 mg/day of APS, 600 mg/day of propolis, or 600 mg/day of the mixture of APS (420 mg/day) and propolis (180 mg/day) for four weeks.

Urinary excretion of BaP and cotinine (a metabolite of nicotine) increased in a time-dependent manner increased after supplementation with APS (BaP, 2.23-fold; cotinine, 2.64-fold), propolis (BaP, 1.30-fold; cotinine, 2.08-fold), and the mixture (BaP, 2.33-fold; cotinine, 2.28-fold) compared with smoker control. Creatinine, glucose, and total bilirubin levels significantly decreased in a time-dependent manner after supplementation with APS (creatinine, 15.24%; glucose, 40.22%; total bilirubin, 48.82%), propolis (creatinine, 16.83%; glucose, 36.25%; total bilirubin, 52.59%), and the mixture (creatinine, 16.36%; glucose, 46.37%; total bilirubin, 39.20%) (p < 0.05).

These results suggest that supplementation with APS, propolis, or the mixture could reduce the risk of cancer or other diseases associated with tobacco smoking by enhancing urinary excretion of BaP and nicotine.

Tuesday, May 21, 2019

Manuka Honey May Help Treat Lung Infections in Those Suffering from Cystic Fibrosis


Anti-pseudomonad Activity of Manuka Honey and Antibiotics in a Specialized ex vivo Model Simulating Cystic Fibrosis Lung Infection

Front Microbiol. 2019 Apr 24;10:869

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Pseudomonas aeruginosa causes problematic chronic lung infections in those suffering from cystic fibrosis. This is due to its antimicrobial resistance mechanisms and its ability to form robust biofilm communities with increased antimicrobial tolerances.

Using novel antimicrobials or repurposing current ones is required in order to overcome these problems. Manuka honey is a natural antimicrobial agent that has been used for many decades in the treatment of chronic surface wounds with great success, particularly those infected with P. aeruginosa. Here we aim to determine whether the antimicrobial activity of manuka honey could potentially be repurposed to inhibit pulmonary P. aeruginosa infections using two ex vivo models. P. aeruginosa isolates (n = 28) from an international panel were tested for their susceptibility to manuka honey and clinically relevant antibiotics (ciprofloxacin, ceftazidime, and tobramycin), alone and in combination, using conventional antimicrobial susceptibility testing (AST).

To increase clinical applicability, two ex vivo porcine lung (EVPL) models (using alveolar and bronchiolar tissue) were used to determine the anti-biofilm effects of manuka honey alone and in combination with antibiotics. All P. aeruginosa isolates were susceptible to manuka honey, however, varying incidences of resistance were seen against antibiotics.

The combination of sub-inhibitory manuka honey and antibiotics using conventional AST had no effect on activity against the majority of isolates tested. Using the two ex vivo models, 64% (w/v) manuka honey inhibited many of the isolates where abnormally high concentrations of antibiotics could not. Typically, combinations of both manuka honey and antibiotics had increased antimicrobial activity.

These results highlight the potential of manuka honey as a future antimicrobial for the treatment of pulmonary P. aeruginosa isolates, clearing potential infection reservoirs within the upper airway.

Monday, May 20, 2019

Today is World Bee Day


About the project World Bee Day

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Slovenia proposed that the United Nations (UN) proclaim 20 May as World Bee Day. On 20 December 2017, following three years of efforts at the international level, the UN Member States unanimously approved Slovenia’s proposal, thus proclaiming 20 May as World Bee Day.

The purpose of the www.worldbeeday.org website is to present the initiative and its implementation, raise awareness of the importance of bees and beekeeping, inform the public of major beekeeping events around the world and celebrate World Bee Day....

Sunday, May 19, 2019

Propolis as an Alternative Antibiotic for Root Canals


An In Vitro Comparative Evaluation of Antimicrobial Efficacy of Propolis, Morinda Citrifolia Juice, Sodium Hypochlorite and Chlorhexidine on Enterococcus faecalis and Candida albicans

J Contemp Dent Pract. 2019 Jan 1;20(1):40-45.

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

AIM:

To evaluate the antimicrobial effectiveness of Propolis, Morinda citrifolia juice, Sodium hypochlorite and Chlorhexidine on Enterococcus feacalis (E. feacalis) and Candida albicans (C. albicans), as endodontic irrigants.

MATERIALS AND METHODS:

Four clinical isolates and one standard ATCC sample (29212) of E. feacalis and ATCC sample (90028) of C. albicans were inoculated into 5ml of peptone water each and incubated at 37° C for 3 to 4 hours to attain the turbidity corresponding to 0.5 McFarland standard CFU. We followed Disc and well diffusion Kirby-Bauer method to attain the zones of inhibition.

RESULTS:

Overall comparison of reagents revealed a significant difference among zones of inhibition. The standard concentration of 5% sodium hypochlorite, 2% chlorhexidine, 10% Propolis and 100% Morinda citrifolia juice illustrated the maximum inhibition zone for both test organisms.

CONCLUSION:

All four reagents had an antimicrobial effect on the microorganisms tested. Sodium hypochlorite and Chlorhexidine were more effectual than Propolis and Morinda citrifolia juice and there was increased antimicrobial efficacy with increasing concentrations.

CLINICAL SIGNIFICANCE:

A study for finding safe herbal agents that can be used as endodontic irrigants revealed that Propolis, Morinda citrifolia juice has antibiotic properties and can replace routinely used agents thereby limiting the side effects.

Saturday, May 18, 2019

Biological Activities of Propolis From Sicily (Italy), Croatia, Malta, Creta (Greece), Turkey, Cyprus, Egypt, Libya, Algeria and Morocco


Insight on propolis from Mediterranean countries chemical composition, biological activities and application fields

Chem Biodivers. 2019 May 17

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

This review updates the information upon the chemical composition of propolis from all Mediterranean countries as well as their biological properties and applications. The non-volatile fraction of propolis was characterized by the presence of phenolic acids and their esters, and flavonoids. Nevertheless in some countries, diterpenes were also present: Sicily (Italy), Croatia, Malta, Creta (Greece), Turkey, Cyprus, Egypt, Lybia, Algeria and Morocco. The volatile fraction of propolis was characterized by the presence of benzoic acid and its esters, mono- and sesquiterpenes, being the oxygenated sesquiterpene β-eudesmol characteristic of poplar propolis, whereas the hydrocarbon monoterpene α-pinene has been related with the presence of conifers. Regardless the chemical composition, there are common biological properties attributed to propolis, Owing to these attributes, propolis has been target of study for applications in diverse areas, such as food, medicine and livestock.

Friday, May 17, 2019

Iranian Propolis Reduces Blood Glucose, Insulin Resistance, May Help Treat Liver and Kidney Dysfunction in Type 2 Diabetes Patients


The Effect of Iranian Propolis on Glucose Metabolism, Lipid Profile, Insulin Resistance, Renal Function and Inflammatory Biomarkers in Patients with Type 2 Diabetes Mellitus: A Randomized Double-Blind Clinical Trial

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Sci Rep. 2019 May 13;9(1):7289

Propolis is a natural product with many biological properties including hypoglycemic activity and modulating lipid profile.

The present study was designed to evaluate the effect of Iranian propolis extract on glucose metabolism, Lipid profile, Insulin resistance, renal and liver function as well as inflammatory biomarkers in patients with type 2 diabetes mellitus (T2DM).

A double-blind, placebo-controlled clinical trial was conducted. The duration of the study lasted 90 days. Patients with T2DM were recruited and randomly divided into an Iranian propolis group (1000 mg/day) (n = 50) and a placebo group (n = 44).

There was a significant decrease in the serum levels of glycosylated hemoglobin (HbA1c), 2-hour post prandial (2hpp), insulin, homeostasis model assessment-insulin resistance (HOMA-IR), homeostasis model assessment of β-cell function (HOMA-β), High sensitive C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α). However, there was a notable elevation in the serum HDL-C in the propolis group compared with the placebo group.

In addition, a notable reduction in serum liver transaminase (ALT and AST) and blood urea nitrogen (BUN) concentrations in the propolis group was observed.

Iranian propolis has beneficial effects on reducing post prandial blood glucose, serum insulin, insulin resistance, and inflammatory cytokines. It is also a useful treatment for preventing the liver and renal dysfunction, as well as, elevating HDL-C concentrations in patients with T2DM.

Thursday, May 16, 2019

Dr. Sanjay Gupta: Could bee stings help treat arthritis?


Dr. Sanjay Gupta travels to Turkey, where doctors like Ali Timucin use bee venom to treat ALS, arthritis and multiple sclerosis. For more, watch "Chasing Life with Dr. Sanjay Gupta" Saturday at 9 p.m. ET/PT.

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Tuesday, May 14, 2019

Pakistani Beri Honey Could Be Alternative Treatment for Infected Wounds


Antibacterial activity of Pakistani Beri honey compared with silver sulfadiazine on infected wounds: a clinical trial

J Wound Care. 2019 May 2;28(5):291-296

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

OBJECTIVE:

To determine the antibacterial activity of Pakistani Beri honey in patients with infected wounds in comparison with silver sulfadiazine.

METHOD:

Inpatients with infected wounds at a tertiary care hospital were divided in to three equal-sized treatment groups. In Group A, patients were treated with non-Gamma irradiated Beri honey. In Group B, Gamma irradiated Beri honey was used, and in Group C silver sulfadiazine was used. Treatment was for a period of four weeks. Pus swabs were taken at day zero and weeks one and four of treatment. Bacteria were identified using the analytical profile index system API 20E, 20NE and API Staph and antimicrobial susceptibility was done as per the Clinical and Laboratory Standards Institute 2010 guidelines.

RESULTS:

A total of 90 patients with wounds (n = 90) took part in the trial. Out of 90 wounds, 47% were post-traumatic and 37% were postoperative. Overall, average length, width and depth of Group A patients' wounds were significantly reduced (p < 0.0001). Out of 144 pus swabs; 99 and 45 were Gram-negative rods and Gram-positive cocci, respectively. Among these Pseudomonas aeruginosa (n = 25) and Staphylococcus aureus (n = 38) were the major pathogens. Interestingly, bacterial load gradually decreased from baseline to week four due to non-Gamma irradiated Beri honey. Moreover, both the Gram-negative rods and Gram-positive cocci displayed 100% resistance to commonly used antibiotics; the most effective drugs were carbapenem and vancomycin.

CONCLUSION:

Pakistani Beri honey could be used as an alternative therapeutic option for the management of infected wounds.

Monday, May 13, 2019

The Surprising Health Benefits of Honey


YAHOO! Lifestyle

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Recent studies have shown that eating honey creates a protective barrier in your throat that may prevent infections. (Honey also has antimicrobial properties.) Boost your immune system and kick your cough with these sweet picks...

Saturday, May 11, 2019

Honey Shows Antibacterial Activity Against Methicillin-Resistant Staphylococcus aureus (MRSA)


Antibacterial Activity of Honey against Methicillin-Resistant Staphylococcus aureus: A Laboratory-Based Experimental Study

Int J Microbiol. 2019 Apr 3;2019:7686130

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Background:

Antimicrobial drug resistance is one of the serious issues this world is facing nowadays, and increased cost of searching for effective antimicrobial agents and the decreased rate of new drug discovery have made the situation increasingly worrisome.

Objective:

The aim of this study is to determine in vitro antibacterial activity of honey against methicillin-resistant Staphylococcus aureus isolates from wound infection.

Methods:

An experimental study was conducted from May to November 2017. Methicillin resistance was detected using cefoxitin (30 μg) and oxacillin (1 μg) antibiotic discs. Different concentrations of honey (25-100% v/v) were tested against each type of clinical isolates obtained from wound infection. A preliminary sensitivity test was done to all types of honey by using disk diffusion while minimum inhibitory concentration and minimum bactericidal concentration were determined for the most potent honey by the broth dilution technique. All statistical analysis was performed by using Statistical Package for the Social Sciences version 20.

Results:

In this study, 36 bacterial isolates were recovered from 50 specimens, showing an isolation rate of 72%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus (15, 41.7%). Among identified Staphylococcus aureus, methicillin resistance accounts for 10 isolates (27.8%). All isolates showed a high frequency of resistance to tetracycline. Four collected honey varieties exhibited antibacterial activity, while the strongest inhibitory activity was demonstrated by honey-2 at 75% v/v. The mean MIC and MBC of honey-2 ranged from 9.38 to 37.5% v/v.

Conclusions:

Tested honey has both a bacteriostatic and bactericidal activity. Among the tested honey, "honey-2" had high antibacterial potency than others.

Friday, May 10, 2019

Sonoran Desert Propolis Inhibits Cancer Tumor Growth

Plant origin authentication of Sonoran Desert propolis: an antiproliferative propolis from a semi-arid region

Naturwissenschaften. 2019 May 8;106(5-6):25

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

The main chemical composition of Sonoran propolis (SP), as well as its antiproliferative activity on cancer cells through apoptosis induction, has been reported. The chemical constitution of SP remained qualitatively similar throughout the year, whereas the antiproliferative effect on cancer cells exhibited significant differences amongst seasonal samples.

The main goal of this study was to provide phytochemical and pharmacological evidence for the botanical source of SP and its antiproliferative constituents. A chemical comparative analysis of SP and plant resins of species found in the surrounding areas of the beehives was carried out by HPLC-UV-DAD, as well as by 1H NMR experiments. The antiproliferative activity on cancerous (M12.C3.F6, HeLa, A549, PC-3) and normal cell lines (L-929; ARPE-19) was assessed through MTT assays. Here, the main polyphenolic profile of SP resulted to be qualitatively similar to Populus fremontii resins (PFR). However, the antiproliferative activity of PFR on cancer cells did not consistently match that exhibited by SP throughout the year.

Additionally, SP induced morphological modifications on treated cells characterised by elongation, similar to those induced by colchicine, and different to those observed with PFR treatment. These results suggest that P. fremontii is the main botanical source of SP along the year. Nevertheless, the antiproliferative constituents of SP that induce that characteristic morphological elongation on treated cells are not obtained from PFR. Moreover, the presence of kaempferol-3-methyl-ether in SP could point Ambrosia ambrosioides as a secondary plant source.

In conclusion, SP is a bioactive poplar-type propolis from semi-arid zones, in which chemical compounds derived from other semi-arid plant sources than poplar contribute to its antiproliferative activity.

Thursday, May 09, 2019

Red Propolis Inhibits Tumor Growth


Red propolis and L-lysine on angiogenesis and tumor growth in a new model of hamster cheek pouch inoculated with Walker 256 tumor cells.

Einstein (Sao Paulo). 2019 May 2;17(2):eAO4576

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

OBJECTIVE:

To evaluate the effect of red propolis and L-lysine on angiogenesis and tumor growth in a new model of hamster cheek pouch inoculated with Walker 256 tumor cells.

METHODS:

The study consisted of two experiments with four groups each (total: 57 hamsters). In the experiment 1, the animals were inoculated with Walker tumor cells, followed by administration of test substances (red propolis 200mg/5mL/kg or L-lysine 150mg/kg) or control substances (gum arabic 5mL/kg or water 5mL/kg) for 10 days. The animals in the experiment 2 received red propolis, L-lysine, gum arabic or water at the same doses, for 33 days prior to inoculation of Walker tumor cells, followed by 10 days of treatment with the same substances. Based on single-plane images, angiogenesis was quantified (mean vascular area), in percentage, and tumor area (mm2) and perimeter (mm).

In the experiment 1, compared to animals receiving water, the mean vascular area expressed in percentage was significantly smaller in animal treated with propolis (p < 0.05) and L-lysine (p < 0.001).

CONCLUSION:

Both red propolis and L-lysine inhibited tumor angiogenesis in the new hamster cheek pouch model when administered after tumor inoculation.

Wednesday, May 08, 2019

Propolis May Help Prevent Blindness


Caffeic acid phenethyl ester attenuates nuclear factor‑κB‑mediated inflammatory responses in Müller cells and protects against retinal ganglion cell death

Mol Med Rep. 2019 Apr 11

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Glaucoma is characterized by the death of retinal ganglion cells (RGCs) and visual field defects, and is a leading cause of blindness worldwide. Caffeic acid phenethyl ester (CAPE), a natural polyphenolic found in propolis from honeybee hives, can inhibit the activation of nuclear factor κ light‑chain‑enhancer of activated B cells (NF‑κB) and has therapeutic potential in inflammatory disease.

The present study used a rat model of optic nerve crush (ONC) injury to investigate the effect of CAPE on glaucoma. The death of RGCs at day 14 was significantly reduced in CAPE‑treated animals compared with the non‑treated group according to Brn3a and TUNEL staining. In addition, CAPE decreased the severity of inflammation in the retina, reflected by the decreased expression of inflammatory cytokines, including interleukin (IL)‑8, IL‑6, inducible nitric oxide synthase, cycloooxygenase‑2, tumor necrosis factor‑α and chemokine C‑C ligand‑2, in CAPE‑treated rats. The hypertrophy of astrocytes and Müller cells (gliosis) caused by ONC was also found to be attenuated by CAPE, accompanied by the inhibition of NF‑κB signaling. Similarly, in vitro,

CAPE suppressed the proliferation and migration of primary astrocytes induced by lipopolysaccharide, as well as the activation of NF‑κB.

These results suggest that CAPE protected against RGC and attenuated inflammatory responses in a rat model of ONC by suppressing NF‑κB activation.

Tuesday, May 07, 2019

Propolis Extract Boosts Wound Healing


Cornstarch-based wound dressing incorporated with hyaluronic acid and propolis: In vitro and in vivo studies

Carbohydr Polym. 2019 Jul 15;216:25-35

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

The unique physicochemical and functional characteristics of starch-based biomaterials and wound dressings have been proposed for several biomedical applications. Film dressings of cornstarch/hyaluronic acid/ ethanolic extract of propolis (CS/HA/EEP) were prepared by solvent-casting and characterized by attenuated total reflectance/Fourier transform infrared spectroscopy, scanning electron microscopy, gas chromatography/mass spectrometry, light transmission, opacity measurements, EEP release, equilibrium swelling, and in vitro and in vivo evaluations.

The CS/HA/0.5%EEP film dressing exhibited higher antibacterial activity against Staphylococcus aureus (2.08 ± 0.14 mm), Escherichia coli (2.64 ± 0.18 mm), and Staphylococcus epidermidis (1.02 ± 0.15 mm) in comparison with the CS, CS/HA, and CS/HA/0.25%EEP films. Also, it showed no cytotoxicity for the L929 fibroblast cells. This wound dressing could effectively accelerate the wound healing process at Wistar rats' skin excisions.

These results indicate that enrichment of cornstarch wound dressings with HA and EEP can significantly enhance their potential efficacy as wound dressing material.

Sunday, May 05, 2019

Ultrasonic Treatment Has Greater Wall-Disruption Effects on Rose (Rosa rugosa) Bee Pollen, Boosts Functional Food Properties


Effect of ultrasonic and ball-milling treatment on cell wall, nutrients and antioxidant capacity of rose (Rosa rugosa) bee pollen, as well as identification of bioactive components

J Sci Food Agric. 2019 May 3

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

BACKGROUND:

Bee pollen has been regarded as a complete nutrition supplement for human diet, but the nutrient absorption and biological activities may be restricted by the complex pollen wall. The aim of this study was to explore the effects of ultrasonic and ball-milling treatment on nutritional components release and antioxidant activities in vitro and in vivo of rose (Rosa rugosa) bee pollen.

RESULTS:

After wall-breaking treatments, the bee pollen walls were broken in varying degrees, nutrients were released and in vitro and in vivo antioxidant activities of bee pollen were improved. The scavenging activities of DPPH and ABTS radicals as well as ORAC were improved. In aging mice, wall-breaking treatments led to better organ recovery, superoxide dismutase (SOD) and catalase (CAT) activities enhancements and malondialdehyde (MDA) reduction. In addition, eight compounds of rose bee pollen ethanol extract including Isorhamnetin 3-O-diglucoside and N', N″, N‴-Dicaffeoyl p-coumaroyl spermidine were identified by UPLC-ESI-QTOF-MS/MS assay.

CONCLUSION:

This study showed that the ultrasonic treatment had greater wall-disruption effects of bee pollen on nutrients release and antioxidant activities promotion. In conclusion, rose bee pollen under wall-breaking treatments may have potential application value as ingredient in functional food processing.

Friday, May 03, 2019

Propolis Flavonoid May Help Treat Chronic Illnesses Such as Cardiovascular Disease, Neurodegenerative Diseases, and Diabetes

Pinocembrin Protects from AGE-Induced Cytotoxicity and Inhibits Non-Enzymatic Glycation in Human Insulin

Cells. 2019 Apr 26;8(5). pii: E385

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Advanced glycation end products (AGEs) are the end products of the glycation reaction and have a great importance in clinical science for their association with oxidative stress and inflammation, which play a major role in most chronic diseases, such as cardiovascular disease, neurodegenerative diseases, and diabetes.

Their pathogenic effects are generally induced by the interaction between AGEs and the receptor for advanced glycation end product (RAGE) on the cell surface, which triggers reactive oxygen species production, nuclear factor kB (NF-kB) activation, and inflammation. Pinocembrin, the most abundant flavonoid in propolis, has been recently proven to interfere with RAGE activation in Aβ-RAGE-induced toxicity. In the present study, we investigated the ability of pinocembrin to interfere with RAGE signaling pathways activated by AGEs.

Interestingly, pinocembrin was able to inhibit oxidative stress and NF-kB activation in cells exposed to AGEs. In addition, it was able to block caspase 3/7 and 9 activation, thus suggesting an active role of this molecule in counteracting AGE-RAGE-induced toxicity mediated by NF-kB signaling pathways. The ability of pinocembrin to affect the glycation reaction has been also tested. Our data suggest that pinocembrin might be a promising molecule in protecting from AGE-mediated pathogenesis.

Thursday, May 02, 2019

Propolis Helps Treat Chronic Kidney Disease in Diabetic Patients


Effects of Brazilian green propolis on proteinuria and renal function in patients with chronic kidney disease: a randomized, double-blind, placebo-controlled trial

BMC Nephrol. 2019 Apr 25;20(1):140

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

BACKGROUND:

Chronic kidney disease (CKD) is a public health problem worldwide, and proteinuria is a well-established marker of disease progression in CKD patients. Propolis, a natural resin produced by bees from plant materials, has anti-inflammatory, immunomodulatory, and anti-oxidant properties, as well as having been shown to have an antiproteinuric effect in experimental CKD. The aim of this study was to evaluate the impact of Brazilian green propolis extract on proteinuria reduction and the changes in the estimated glomerular filtration rate (eGFR).

METHODS:

This was a randomized, double-blind, placebo-controlled study including patients with CKD caused by diabetes or of another etiology, 18-90 years of age, with an eGFR of 25-70 ml/min per 1.73 m2 and proteinuria (urinary protein excretion > 300 mg/day) or micro- or macro-albuminuria (urinary albumin-to-creatinine ratio > 30 mg/g or > 300 mg/g, respectively). We screened 148 patients and selected 32, randomly assigning them to receive 12 months of Brazilian green propolis extract at a dose of 500 mg/day (n = 18) or 12 months of a placebo (n = 14).

RESULTS:

At the end of treatment, proteinuria was significantly lower in the propolis group than in the placebo group-695 mg/24 h (95% CI, 483 to 999) vs. 1403 mg/24 h (95% CI, 1031 to 1909); P = 0.004-independent of variations in eGFR and blood pressure, which did not differ between the groups during follow-up. Urinary monocyte chemoattractant protein-1 was also significantly lower in the propolis group than in the placebo group-58 pg/mg creatinine (95% CI, 36 to 95) vs. 98 pg/mg creatinine (95% CI, 62 to 155); P = 0.038.

CONCLUSIONS:

Brazilian green propolis extract was found to be safe and well tolerated, as well as to reduce proteinuria significantly in patients with diabetic and non-diabetic CKD.

Wednesday, May 01, 2019

Algerian Propolis Protects Heart from Oxidative Damage


Myocardial protection by propolis during prolonged hypothermic preservation

Cryobiology. 2019 Apr 11. pii: S0011-2240(19)30009-4

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Oxidative stress is involved in the pathogenesis of ischemia-reperfusion during myocardial transplantation. Therefore, graft preservation solutions may be improved by supplementation with antioxidants to minimize graft dysfunction caused by cold ischemic injury.

Propolis is a polyphenol-rich substance which has an important antioxidant activity. The protective effect of propolis against oxidative stress induced by prolonged cold preservation of heart was investigated. Mice were subjected to a hypothermic model of ischemia in which hearts were preserved for 24 h at 4 °C in Krebs-Hensleit (KH) solution in the absence or presence of propolis concentrations (50, 150 and 250 μg/ml). Levels of released Lactate dehydrogenase (LDH), Creatine phosphokinase (CPK) and Troponine-I (Trop I) were assessed in the preservation solution and histological assessement of heart ischemia injuries was performed. Oxidative stress biomarkers malondialdehyde (MDA) and advanced oxidation protein products (AOPP) and antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) were assessed in cardiac tissue.

Mitochondria were isolated from stored hearts and production of reactive oxygen species (ROS) was tested. Propolis supplementation protected efficiently hearts during preservation by reducing significantly levels of lipids and proteins oxidation and restoring activities of antioxidant enzymes. Also, propolis preserved tissue integrity altered by hypothermic ischemia in a concentration-dependent manner.

Propolis reduced significantly the rate of H2O2 produced by mitochondrial respiration, the best antioxidant effect being obtained at the highest propolis concentration (250 μg/ml). Algerian propolis is a non-temperature sensitive scavenger that protects heart from oxidative damage induced by prolonged hypothermic ischemia.

Monday, April 22, 2019

Beeswax Helps Treat Moderate to Severe Rosacea

Natural Skin Care Products as Adjunctive to Prescription Therapy in Moderate to Severe Rosacea

J Drugs Dermatol. 2019 Feb 1;18(2):141-146.

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

BACKGROUND:

Rosacea is characterized by irritation associated with erythema, telangiectasias and papules/pustules. Whole formula nature-based sensitive skin products are formulated to maintain skin barrier and appropriate hydration that can lead to soothing benefits.

OBJECTIVE:

To evaluate the efficacy and tolerability of a regimen consisting of a cleanser containing natural oils, beeswax, and witch hazel and day and night creams containing natural oils, glycerin, and botanical anti-inflammatories (NR); and a synthetic dermatologist-recommended regimen of cetyl alcohol, sodium lauryl sulphate-containing cleanser, and glycerin, polyisobutene-containing lotion (CR) in subjects with rosacea.

METHODS:

80 female subjects with rosacea who received 6 weeks of 0.75% metronidazole gel, were randomized to receive NR or CR, twice daily, for 4 weeks in conjunction with the gel. Blinded investigator global assessment of rosacea, investigator-rated, and subject-rated overall skin appearance was assessed using a 5-point scale (0=none, 4=severe) at baseline, 2 weeks, and 4 weeks. Noninvasive skin assessments for skin hydration and skin barrier function were made by corneometry and TEWL, respectively...

Sunday, April 21, 2019

Royal Jelly Prevents Damage to Kidneys

Royal jelly attenuates cadmium-induced nephrotoxicity in male mice

Sci Rep. 2019 Apr 9;9(1):5825

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Cadmium exposure induces nephrotoxicity by mediating oxidative stress, inflammation, and apoptosis. The purpose of this study was to examine the protective effect of royal jelly on Cd-induced nephrotoxicity. Adult male mice were distributed randomly into 4 clusters: untreated, royal jelly-treated (85 mg/kg, oral), CdCl2-treated (6.5 mg/kg, intraperitoneal), and pretreated with royal jelly (85 mg/kg) 2 h before CdCl2 injection (6.5 mg/kg, intraperitoneal) for seven consecutive days. Cd concentration in the renal tissue and absolute kidney weight of the Cd-treated mice were significantly higher than those of control group.

The levels of kidney function markers, kidney injury molecules-1 (KIM-1), metallothionein, lipid peroxidation, nitric oxide, tumor necrosis factor-α, interleukin-1β, and the apoptosis regulators Bax and caspases-3 also increased significantly in the renal tissue of Cd-treated mice, whereas the levels of glutathione, antioxidant enzyme activities, and the apoptosis inhibitor Bcl-2 were significantly reduced in the renal tissue of Cd-treated group. Histopathological studies showed vacuolation and congested glomeruli in the kidney tissue of Cd-treated mice. However, all aforementioned Cd-induced changes were attenuated by pretreatment with royal jelly.

We therefore concluded that royal jelly attenuated Cd-induced nephrotoxicity and it is suggested that this nephroprotective effect could be linked to its ability to promote the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant responsive element (ARE) pathway.

Saturday, April 20, 2019

Honey Boosts Healing of Colon Anastomosis

Effect of a honey and arginine-glutamine-hydroxymethylbutyrate mixture on the healing of colon anastomosis in rats immunosuppressed with tacrolimus

Biotech Histochem. 2019 Apr 15:1-8

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

We compared the effect of honey and a mixture of arginine-glutamine-hydroxymethylbutyrate (AGHMB) on healing of a descending colon anastomosis in rats that were immunosuppressed with tacrolimus (Tac).

Sprague-Dawley rats were divided into four groups: untreated control, Tac, Tac + honey and Tac + AGHMB. Colon resection and anastomosis were performed on day 14 and re-laparotomy was performed on the day 21 of the study. Anastomotic bursting pressure, macroscopic adhesion score, weekly body weight changes, histopathological features and immunohistochemical staining of TGF-β1 were determined for all groups. We found no significant difference in anastomotic bursting pressure among the experimental groups. We found significant weekly increases in body weight for the Tac + honey group.

We found no significant difference in the weekly body weight measurements for the Tac + AGHMB group. We found significant increases in TGF-β1 expression in the Tac + honey group compared to the control and Tac groups. No significant differences in inflammatory cell infiltration, fibroblast proliferation or collagen deposition were found between the Tac + honey and Tac + AGHMB groups; however, a significant difference in neovascularization between these groups was found. Neovascularization in the Tac + honey group was significantly greater than for the Tac + AGHMB group.

We found that both honey and the AGHMB mixture were beneficial for anastomotic wound healing in rats that were immunosuppressed using Tac.

Friday, April 19, 2019

How are raw honey and regular honey different?

Studies show that raw honey contains a variety of beneficial ingredients.

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Raw honey contains specific components that can offer health benefits. Pasteurization and other processes may remove or reduce some of these elements, which include:

  • bee pollen, which has antioxidant and anti-inflammatory properties
  • bee propolis, a glue-like substance that helps keep the hive together
  • certain vitamins and minerals
  • enzymes
  • amino acids
  • antioxidants...

Thursday, April 18, 2019

Honey-Impregnated Dressings Significantly Reduced Healing Time of Non-Healing Wounds in Home Care Setting

Applying honey dressings to non-healing wounds in elderly persons receiving home care

Journal of Tissue Viability
Available online 13 April 2019

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Highlights

• Honey is one of naturally occurring remedies that can be used in the treatment of non-healing wounds.
• Honey-impregnated dressings significantly reduced the healing time of non-healing wounds in home care setting.
• Honey-impregnated dressings reduced wound pain intensity in elderly.

Objective

The study aimed to determine the clinical effectiveness of honey dressings in the management of non-healing wounds in elderly persons receiving home care.

Material and methods

Design: a prospective interventional study. The sample comprised 40 Czech home care clients (aged over 65 years) with non-healing wounds who were randomly assigned to two groups. Wounds were treated with honey (intervention group) or conventional (controls) dressings. Each wound was studied for three months. A detailed description of a wound (location, size, wound bed, edges, amount of exudate, odor, adjacent skin) were recorded. Wounds were assessed with the Wound Healing Continuum and pain intensity with the Visual Analog Scale.

Results

Over the 3-month period, 16 (80%) individuals in the intervention group had their wounds completely healed, as compared with only six (30%) controls. There was no statistically significant difference in wound size between the groups on Day 1 (p = 0.1801). Ninety days later, the difference in wound size between the groups was statistically significant (p = 0.0041). There was a statistically significant difference in pain intensity between the two groups (p = 0.0007), with higher pain scores being indicated by controls.

Conclusion

The study results showed that the application of honey dressings to non-healing wounds resulted in faster healing, wound size reduction and lower pain intensity.

Wednesday, April 17, 2019

Red Propolis Alagoas Shows Significant Antimicrobial Activity

Braz. J. Biol. vol.79 no.3 São Carlos July/Sept. 2019

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei.

The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

Tuesday, April 16, 2019

South African Propolis Boosts Antimicrobial Activity, Minimizes Cell Damage

The new buzz: Investigating the antimicrobial interactions between bioactive compounds found in South African propolis

J Ethnopharmacol. 2019 Apr 9:111867

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

ETHNOPHARMACOLOGICAL RELEVANCE:

Propolis, a resinous substance produced by the Apis mellifera bee, contains a number of flavonoids sourced from plants found in the surrounding region. Whilst bees use this substance to seal off and protect the beehive, humans have used propolis therapeutically for centuries, making use of its antibacterial, antiseptic, antipyretic and wound healing properties, among others. South African propolis is rich in the flavonoids pinocembrin, galangin, and chrysin and very little previous research has been conducted on the antimicrobial effects of these compounds.

AIM OF THE STUDY:

To obtain an understanding of the antimicrobial activity of the compounds pinocembrin, galangin, and chrysin, both independently and in combination.

MATERIALS AND METHODS:

The compounds pinocembrin, galangin and chrysin were investigated for interactive antimicrobial activity by determining the minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC), anti-quorum sensing activity, biofilm studies, and cytotoxicity studies (brine shrimp lethality assay).

RESULTS:

Minimum inhibitory concentration results demonstrated that combinations of compounds showed better inhibitory activity than single compounds. When the flavonoids were tested in combination using the MIC assay, synergy was noted for 22% of the 1:1 ratio combinations and for 66% of the triple 1:1:1 ratio combinations. Similarly, MBC results showed bactericidal activity from selected combinations, while the compounds on their own demonstrated no cidal activity. Quorum sensing studies showed that compound combinations are more effective at inhibiting bacterial communication than the individual compounds. Biofilm assays showed that the highest percentage inhibition was observed for the triple combination against E. coli at 24 h. Finally, brine shrimp lethality studies revealed that combinations of the three compounds had reduced cytotoxicity when compared to the individual compounds.

CONCLUSION:

The results obtained in this study demonstrate that the compounds found in South African propolis work synergistically to achieve an optimal antimicrobial effect, whilst simultaneously minimizing cytotoxicity.

Monday, April 15, 2019

Propolis Protects the Heart From Damage Caused by Prolonged Hypothermic Preservation

Myocardial protection by propolis during prolonged hypothermic preservation.

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Cryobiology. 2019 Apr 11. pii: S0011-2240(19)30009-4

Oxidative stress is involved in the pathogenesis of ischemia-reperfusion during myocardial transplantation. Therefore, graft preservation solutions may be improved by supplementation with antioxidants to minimize graft dysfunction caused by cold ischemic injury. Propolis is a polyphenol-rich substance which has an important antioxidant activity. The protective effect of propolis against oxidative stress induced by prolonged cold preservation of heart was investigated. Mice were subjected to a hypothermic model of ischemia in which hearts were preserved for 24 h at 4 °C in Krebs-Hensleit (KH) solution in the absence or presence of propolis concentrations (50, 150 and 250 μg/ml). Levels of released Lactate dehydrogenase (LDH), Creatine phosphokinase (CPK) and Troponine-I (Trop I) were assessed in the preservation solution and histological assessement of heart ischemia injuries was performed. Oxidative stress biomarkers malondialdehyde (MDA) and advanced oxidation protein products (AOPP) and antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) were assessed in cardiac tissue. Mitochondria were isolated from stored hearts and production of reactive oxygen species (ROS) was tested. Propolis supplementation protected efficiently hearts during preservation by reducing significantly levels of lipids and proteins oxidation and restoring activities of antioxidant enzymes. Also, propolis preserved tissue integrity altered by hypothermic ischemia in a concentration-dependent manner. Propolis reduced significantly the rate of H2O2 produced by mitochondrial respiration, the best antioxidant effect being obtained at the highest propolis concentration (250 μg/ml). Algerian propolis is a non-temperature sensitive scavenger that protects heart from oxidative damage induced by prolonged hypothermic ischemia.

Friday, April 12, 2019

Royal Jelly May Help Treat Diabetes


Effects of Royal jelly on metabolic variables in diabetes mellitus: A systematic review.

Complement Ther Med. 2019 Apr;43:20-27

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Diabetes mellitus is one of the most common endocrine disorders in the world. This systematic review was conducted with focus on the current knowledge on the effect of royal jelly on metabolic variables in diabetes mellitus.

PubMed, Scopus, Embase, ProQuest and Google Scholar databases were searched from inception until June 2018. All clinical trials and animal studies that evaluated the effects of royal jelly on diabetes mellitus, and were published in English-language journals were eligible. Studies that provided insufficient outcomes were excluded. Out of 522 articles found in the search, only twelve articles were eligible for analysis. Seven studies showed a significant reduction in FBS, and one reported HbA1c decrease following royal jelly supplementation.

Although royal jelly supplementation resulted in significant reductions in HOM A-I R in three studies, the findings on insulin levels were controversial. In addition, royal jelly substantially improved serum levels of triglycerides, cholesterol, HDL, LDL, VLDL and Apo-A1 in diabetes mellitus. In addition, royal jelly resulted in a decrease oxidative stress indicators and increase antioxidant enzymes levels.

In conclusion, royal jelly could improve glycemic status, lipid profiles and oxidative stress in diabetes mellitus. However, exploring the underlying mechanisms warrants further studies.

Thursday, April 11, 2019

Honey Shows Bacteriostatic and Bactericidal Activity Against MRSA - Methicillin-Resistant Staphylococcus aureus

Antibacterial Activity of Honey against Methicillin-Resistant Staphylococcus aureus: A Laboratory-Based Experimental Study

International Journal of Microbiology
Volume 2019, Article ID 7686130, 9 pages

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Background. Antimicrobial drug resistance is one of the serious issues this world is facing nowadays, and increased cost of searching for effective antimicrobial agents and the decreased rate of new drug discovery have made the situation increasingly worrisome.

Objective. The aim of this study is to determine in vitro antibacterial activity of honey against methicillin-resistant Staphylococcus aureus isolates from wound infection.

Methods. An experimental study was conducted from May to November 2017. Methicillin resistance was detected using cefoxitin (30 μg) and oxacillin (1 μg) antibiotic discs. Different concentrations of honey (25–100% v/v) were tested against each type of clinical isolates obtained from wound infection. A preliminary sensitivity test was done to all types of honey by using disk diffusion while minimum inhibitory concentration and minimum bactericidal concentration were determined for the most potent honey by the broth dilution technique. All statistical analysis was performed by using Statistical Package for the Social Sciences version 20.

Results. In this study, 36 bacterial isolates were recovered from 50 specimens, showing an isolation rate of 72%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus (15, 41.7%). Among identified Staphylococcus aureus, methicillin resistance accounts for 10 isolates (27.8%). All isolates showed a high frequency of resistance to tetracycline. Four collected honey varieties exhibited antibacterial activity, while the strongest inhibitory activity was demonstrated by honey-2 at 75% v/v. The mean MIC and MBC of honey-2 ranged from 9.38 to 37.5% v/v.

Conclusions. Tested honey has both a bacteriostatic and bactericidal activity. Among the tested honey, “honey-2” had high antibacterial potency than others.

Wednesday, April 10, 2019

Dietary Propolis Supplementation Boosts Egg Production


Propolis alleviates the negative effects of heat stress on egg production, egg quality, physiological and immunological aspects of laying Japanese quail.

PLoS One. 2019 Apr 9;14(4):e0214839

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

The present work was carried out to investigate the effects of dietary propolis supplementation to laying Japanese quail (Coturnix coturnix japonica) on egg production, egg quality, physiological and immunological aspects under heat stress conditions. A total of 200, 21-day-old, Japanese quail females were distributed equally into standard wired cages in two identical environmentally-controlled rooms (10 cages per room, 10 birds per cage). From 29-70 d of age, the quail birds in the first room remained at a normal temperature of 24°C (C group), whereas the quail birds in the second room were kept under heat stress at 35°C (HS group).

Each group was further assigned to 2 propolis subgroups (5 cages per subgroup); one of them received a basal diet without propolis supplementation (-PR subgroup), while, the other received 1 g propolis/ kg basal diet (+PR subgroup). In the present study, performance and egg production of laying quail were significantly (P < 0.001) impaired by HS treatment and improved by the PR treatment. Similarly, the negative and positive effects of HS and PR, respectively, were appeared on the egg shell thickness and yolk index. Stress indicators in laying quail were significantly (P < 0.001) increased by HS, while, PR significantly (P < 0.05) moderated these levels in the HS+PR group when compared to the HS-PR quail group.

In addition to the positive impact of PR on the plasma levels of calcium, phosphorus, and albumin, it also normalized the plasma levels of alanine aminotransferase and cholesterol in the heat-stressed quail birds. Moreover, the quail birds in the HS groups expressed lower immunological aspects than those in the C group, while, the addition of propolis to the diets enhanced the immune status of laying quail birds under HS conditions.

These results strongly suggest that dietary propolis supplementation could be a successful attempt to maintain the performance and egg production of laying Japanese quail at convenient levels under heat stress conditions.

Tuesday, April 09, 2019

Pinocembrin, a Flavanone Found in Honey, Exhibited Strongest Inhibition of Zika Virus Replication


Antiviral activity of pinocembrin against Zika virus replication.

Antiviral Res. 2019 Apr 5. pii: S0166-3542(18)30601-6

BUY Concentrated Propolis in Veggie Capsules  

BUY 3-Piece (2 Fountain Pens, Rollerball) Gift Set 

Zika virus (ZIKV) is a mosquito-borne virus that has garnered a lot of attention in recent years, due to the explosive epidemic from 2014 to 2016. Since its introduction in the Americas in late 2014, ZIKV has spread at an unprecedented rate and scale throughout the world and infected millions of people. Its infection has also been associated with severe neurological disorders like Guillain-Barré syndrome and microcephaly in fetuses. Despite these, there is currently no approved antiviral against ZIKV. In this study, an immunofluorescence-based high throughput screen was conducted on a library of 483 flavonoid derivatives to identify potential anti-ZIKV compounds. Flavonoids, which are natural polyphenolic compounds found in plants, represent an attractive source of antivirals due to their abundance in food and expected low toxicity. From the primary screen, three hits were selected for validation by cell viability and viral plaque reduction assays. Pinocembrin, a flavanone found in honey, tea and red wine, was chosen for downstream studies as it exhibited the strongest inhibition of ZIKV infection in human placental JEG-3 cells (IC50 = 17.4 μM). Time-course studies revealed that pinocembrin acts on post-entry process(es) of the ZIKV replication cycle. Furthermore, pinocembrin inhibits viral RNA production and envelope protein synthesis based on quantitative reverse transcription-PCR (qRT-PCR) and Western blot analyses. This study has demonstrated for the first time the in vitro anti-ZIKV activity of pinocembrin.