Wednesday, January 13, 2021

Malaysian Tualang, Gelam and Kelulut Honeys Show Anti-Diabetic, Anti-Obesity, Anti-Cancer, Anti-Oxidative, Anti-Microbial, Anti-Inflammatory, and Wound-Healing Properties

Physicochemical and Medicinal Properties of Tualang, Gelam and Kelulut Honeys: A Comprehensive Review

Nutrients. 2021 Jan 10;13(1):E197

Tualang, Gelam and Kelulut honeys are tropical rainforest honeys reported to have various medicinal properties. Studies related to the medicinal properties and physicochemical characteristics of these honeys are growing extensively and receiving increased attention. This review incorporated and analysed the findings on the biological and physicochemical properties of these honeys. Tualang, Gelam and Kelulut honeys were found to possess a wide variety of biological effects attributed to their physicochemical characteristics. 

Findings revealed that these honeys have anti-diabetic, anti-obesity, anti-cancer, anti-oxidative, anti-microbial, anti-inflammatory and wound-healing properties and effects on the cardiovascular system, nervous system and reproductive system. The physicochemical properties of these honeys were compared and discussed and results showed that they have high-quality contents and excellent antioxidant sources.

Sunday, January 10, 2021

Green and Red Brazilian Propolis May Help Treat Heart (Cardiovascular) Disease (High Blood Pressure, Hardening of the Arteries)

The Cardiovascular Therapeutic Potential of Propolis—A Comprehensive Review

Biology 2021, 10(1), 27

Simple Summary

Propolis, also described as bee glue, is a natural component made up of a resinous mixture of honeybee compounds from multiple botanical sources. The literature has demonstrated a variety of medicinal properties attributed to propolis due to its chemical complexity. However, the positive effects of propolis on cardiovascular health have gained little coverage. Therefore, we aimed to provide an accurate and up-to-date review of the main cardiovascular health benefits of propolis. In particular, we intend to establish the key varieties of propolis and pharmacological compounds with the therapeutic effects that are most encouraging, as well as the physiological processes by which those advantages are accomplished.

The Brazilian green and red varieties reveal the greatest number of beneficial activities among the varieties of propolis studied. While much of the cardiovascular beneficial effects appear to derive from the cumulative actions of several compounds working via multiple signaling mechanisms, some individual compounds that may enhance the existing therapeutic arsenal have also shown significant results. It is also worth exploring the prospect of using propolis as food supplements.


Owing to its chemical richness, propolis has a myriad of therapeutic properties. To the authors’ knowledge, this is the first comprehensive review paper on propolis to focus exclusively on its major effects for cardiovascular health. The propolis compound varieties with the most promising therapeutic benefits and their respective physiological mechanisms will be discussed. Propolis displays an anti-atherosclerotic activity, attained through modulation of the plasma lipid profile and through stabilization of the fatty plaque by inhibiting macrophage apoptosis, vascular smooth muscle proliferation and metalloproteinase activity.

The antihypertensive effects of propolis probably arise through the combination of several mechanisms, including the suppression of catecholamine synthesis, stimulation of endothelium-dependent vasorelaxation and vascular anti-inflammatory activity. The anti-hemostatic activity of propolis is attributed to the inhibition of platelet plug formation and antifibrinolytic activity. By inhibiting the secretion of proangiogenic factors, propolis suppresses endothelial cell migration and tubulogenesis, exerting antiangiogenic activity. The antioxidant and anti-inflammatory activities are responsible for protection against vascular endothelial and cardiomyocyte dysfunction, mostly by the prevention of oxidative stress. Among the reviewed propolis varieties, the Brazilian green and red varieties show the largest number of beneficial activities. Further research, especially preclinical, should be conducted to assess the cardiovascular benefits of the given varieties with different compositions.

Tuesday, January 05, 2021

Propolis May Help Treat Diabetes, Obesity, Cancer (Cytokines, Inflammation, Fat, Diet, Cholesterol, COVID-19)

Biomedical Properties of Propolis on Diverse Chronic Diseases and Its Potential Applications and Health Benefits


The use of alternative medicine products has increased tremendously in recent decades and it is estimated that approximately 80% of patients globally depend on them for some part of their primary health care. Propolis is a beekeeping product widely used in alternative medicine. It is a natural resinous product that bees collect from various plants and mix with beeswax and salivary enzymes and comprises a complex mixture of compounds. Various biomedical properties of propolis have been studied and reported in infectious and non-infectious diseases. However, the pharmacological activity and chemical composition of propolis is highly variable depending on its geographical origin, so it is important to describe and study the biomedical properties of propolis from different geographic regions. A number of chronic diseases, such as diabetes, obesity, and cancer, are the leading causes of global mortality, generating significant economic losses in many countries. In this review, we focus on compiling relevant information about propolis research related to diabetes, obesity, and cancer. The study of propolis could generate both new and accessible alternatives for the treatment of various diseases and will help to effectively evaluate the safety of its use...


Traditional and alternative medicine is the world’s oldest form of health care and is used in the prevention and treatment of physical and mental illnesses in the present day. Propolis is a beekeeping product widely used in alternative medicine due to its easy accessibility. In this review, we compiled some of the biomedical properties of propolis, focusing mainly on diabetes, obesity, and cancer. Our search for information reflects the global trend to seek new alternatives for the treatment of these diseases. There is a significant volume of research on propolis that shows that it is able to help in the control of diabetes by lowering glucose levels, MDA, and pro-inflammatory cytokines, and assisting in weight loss. Furthermore, it enhances serum insulin levels, the translocation of GLUT-4, and the function of antioxidant enzymes, in addition to protecting cells and pancreatic function. In obesity, propolis has also shown benefits, with in vitro and animal models providing evidence that it induces the transcription of adiponectin and leptin, reduces the mass of visceral adipose tissue, and regulates the levels of triglycerides, non-esterified fatty acids, and cholesterol. Another relevant aspect for propolis is the data obtained on its antitumor effects related to the inhibition of the cell cycle, apoptosis, proliferation, viability, growth, and cell migration; it is notable that many of these activities continue to have selectivity towards tumor cells without affecting non-tumor cells. It is necessary to emphasize that the propolis of each geographical region has different biomedical activities due to the significant diversity that exists in its chemical composition. As a result, each geographical variant of propolis can be considered a great source of natural products, particularly terpenes and phenolic compounds, such as flavonoids. Each propolis is thus unique, requiring individual study. Despite all the attributes and virtues of propolis, several challenges remain to overcome. The first is to determine a classification in which all the properties that have been studied can be integrated as well as those that emerge from future investigations. The proposal by Bankova (2005) can function as basis for achieving adequate classification [6]. The standardization of propolis will lead to its safe and adequate consumption, to achieve this it is necessary to generate a commitment from the scientific community that works with this beekeeping product to describe the chemical composition of all the propolis extracts used in any research. Another challenge is to increase in vivo and clinical studies, since much of the available evidence of the biomedical properties of propolis is in vitro work, which, in many cases, prevents the reported activities from being directly applied in humans. Furthermore, we consider that future clinical studies should use propolis with a well-established chemical composition, since this will allow the establishment of a specific dose for each disease and the adequate treatment in infectious and non-infectious diseases. Investigation and better understanding of the properties of propolis, and phenomena such as synergism and other mechanisms of natural products, can assist in the development of new and better medicines and safe consumption treatments as complementary therapies for these diseases.

Saturday, January 02, 2021

Propolis Used to Treat Gynaecological and Gastrointestinal Disorders, Cancer

...1. Helps fight cancer

One of the benefits of the bee propolis is that it has anti-cancer and anti-tumoral properties. Scientific studies are focusing on cancer treatment and propolis. Two propolis polyphenols in specific appear to be the most-effective and powerful antitumour agents. Scientists consider that propolis’ potential to obstruct cancer from growing in both human cell and animal models is probably the outcome of its capability to prevent DNA synthesis in tumour cells and its ability to persuade apoptosis of tumour cells.

2. Gastrointestinal disorder

The infestations caused by parasites usually occur in association with an infected area. The symptoms of the gastrointestinal tract infection consist of diarrhoea, nausea, abdominal pain, and bloating. Propolis has various advantages involving anti-inflammatory and antioxidant properties. Some studies reported that the clinical usage of bee propolis helps in treating viral infections. Research showed it to obstruct adherence and development of trophozoites. It prompted the separation of these parasitic structures.

3. Oral health

Excessive bacterial microflora is present in the oral cavity, and enormous bacterial growth can lead to various oral diseases. Several studies have found that propolis restricts periodontitis-causing pathogens and bacterial-plaque growth owing to its antibacterial activities. Bee propolis extract utilizes a selectively lesser cytotoxic work on human gum fibroblasts. It also helps in healing surgical injuries or wounds. Propolis extract also plays a vital role as a therapeutic agent.

4. Gynaecological care

The causes of vaginitis are vulvovaginal candidiasis and bacterial vaginosis. The reduction of lactobacillus in the vagina is a notable aspect of vaginal infections. This infection is associated with excessive development of vaginal pathogens. A study showed that the use of propolis extract resulted in an enhancement in the well-being of the vagina. It also provides symptomatic relief because of its anesthetic properties. Therefore, one can use propolis as an alternative for patients who cannot take antibiotics because of concurrent pharmacological treatment.

5. Oncological treatment

Propolis is useful in cancer treatment because of its antitumour property by activating apoptosis on cancer cells. While it kills cancerous cells, unlike chemotherapy, it does not harm the cells around it. Also, propolis minimizes the proliferation of cancer cells. Overall, it is beneficial in cancer treatments if used efficiently.

Wednesday, December 23, 2020

Stingless Bee Honey May Help Relieve Symptoms and Problems of Autism Spectrum Disorder (ASD)

In Vitro Modulation of Endogenous Antioxidant Enzyme Activities and Oxidative Stress in Autism Lymphoblastoid Cell Line (ALCL) by Stingless Bee Honey Treatment

Oxid Med Cell Longev. 2020 Dec 1;2020:4539891

Autism has been associated with a low antioxidant defense mechanism, while honey has been known for decades for its antioxidant and healing properties. Determination of stingless bee honey (KH) effects on antioxidant enzyme activities and oxidative damage in Autism Lymphoblastoid Cell Line (ALCL) was performed. 

ALCL and its normal sibling pair (NALCL) were cultured in RPMI-1640 medium at 37°C and 5% CO2. ALCL was treated with 400 μg/mL KH (24 h), and oxidative stress marker, malondialdehyde (MDA), and antioxidant enzyme activities (catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD)) were measured via enzyme-linked immunosorbent assay (ELISA), while deoxyribonucleic acid (DNA) damage was determined via comet assay. 

Low SOD activity (p < 0.05) and high MDA level (p < 0.05) were observed in ALCL compared to NALCL. Higher grade (Grades 2 and 3) of DNA damage was highly observed (p < 0.05) in ALCL compared to NALCL, whereas lower grade (Grades 0 and 1) DNA damage was highly detected (p < 0.05) in NALCL compared to ALCL. KH treatment caused a significant increase in SOD and GPx activities (p < 0.05) in ALCL compared to untreated ALCL. 

Correspondingly, KH treatment reduced the Grade 2 DNA damage (p < 0.05) in ALCL compared to untreated ALCL. CAT activity showed no significant difference between all three groups, while the MDA level showed no significant difference between treated and untreated ALCL. 

In conclusion, KH treatment significantly reduced the oxidative stress in ALCL by increasing the SOD and GPx antioxidant enzyme activities, while reducing the DNA damage...


KH, an antioxidant-rich natural product, has a high potential in alleviating oxidative stress, reducing the oxidative damage, and may help to improve the symptoms and problems of ASD.

Saturday, December 12, 2020

Propolis and Bee Pollen May Help Treat High Cholesterol (Excess Triglycerides) and Liver Damage Caused by Type-2 Diabetes

Effect of antioxidant-rich propolis and bee pollen extracts against D-glucose induced type 2 diabetes in rats

  • Propolis and bee pollen have high antioxidant ability.
  • Naringin and Apigenin were the most abundant polyphenolic compounds in propolis and bee pollen, respectively.
  • 16 weeks of treatment with D-glucose led to type 2-diabetes, insulin resistance, dyslipidemia and hepato-renal damage.
  • Propolis and bee pollen attenuated the deleterious effects induced by D-glucose.
The present study was designed to investigate the preventive effect of propolis, bee pollen and their combination on Type 2 diabetes induced by D-glucose in rats. 

The study was carried out by feeding daily two concentrations (100 and 200 mg/Kg BW) of propolis or bee pollen (or their combination to normal (non-diabetic) and diabetic rats for a period of 16 weeks. In vivo biochemical changes associated to diabetes are induced by drinking a solution containing 10% of D-glucose (diabetic rats). The in vitro antioxidant activity was also evaluated and the chemical composition of propolis and bee pollen extracts was determined by UHPLC-DAD. 

Phytochemical composition of propolis and bee pollen revealed the presence of several natural antioxidants, such as hydroxycinnamic acids, hydroxybenzoic acids, flavonoids, flavan-3-ols and stilbens. The major antioxidant compound present in propolis was Naringin (290.19 ± 0.2 mg/Kg) and in bee pollen was apigenin (162.85 ± 17.7 mg/Kg). 

These results have been related with a high antioxidant activity, more intense in propolis extract. In rats, the administration of D-glucose had induced hyperglycemia (13.2 ± 0.82 mmol/L), increased plasmatic insulin levels (25.10 ± 2.12 U/L) and HOMA-IR index (14.72 ± 0.85) accompanied with dyslipidemia, elevation of hepatic enzyme levels, and a change in both serum renal biomarkers and plasmatic calcium. 

The co-administration of propolis and bee pollen extracts alone or in combination restored these biochemical parameters and attenuated the deleterious effects of D-glucose on liver and kidney functions. Furthermore, these effects were better attenuated in the combined therapy-prevented diabetic rats. Hence, it is possible to conclude that propolis and bee pollen can be used as a preventive natural product against diabetes induced dyslipidemia and hepato-renal damage.

Tuesday, December 08, 2020

Red Propolis May Be Used to Treat Schistosomiasis (Snail Fever, Bilharzia)

In vitro studies and experiments with mice show that the natural extract was more effective than the only drug available to combat this parasitic disease.

Well-known for its bactericidal and anti-fungal properties, Brazilian red propolis has now been found to act powerfully against the parasite that causes schistosomiasis, reducing the number of eggs and killing the helminths (worms).

In experiments performed at Universidade Guarulhos (UnG) in the state of São Paulo, Brazil, with FAPESP’s support, 400 milligrams per kilogram of body weight was a sufficient dose of red propolis to reduce the parasite load by more than 60% in mice infected with the flatworm Schistosoma mansoni. It was equally effective against the immature and adult stages of the parasite. In vitro tests also showed that red propolis blocked reproduction and oviposition (egg-laying).

“Propolis, especially the red variety, is well-known for its action against bacteria and fungi. It protects the beehive from intruders, and we expected that some of its more than 20 substances would act against parasitic infectious agents. What surprised us was that it disrupted the worm’s integument and killed adult as well as immature worms, which the conventional treatment for schistosomiasis doesn’t do,” said Josué de Moraes, a professor at UnG and last author of the article on the study published in the Journal of Ethnopharmacology.

The results of all the tests suggest red propolis may be more effective to treat schistosomiasis than the only pharmaceutical product available for this purpose. Before red propolis can be prescribed for patients, of course, it must be tested in clinical trials on humans with the disease...

Thursday, December 03, 2020

Heat Treatment Decreases Antibacterial Activity of Honey

Antimicrobial activity of heat-treated Polish honeys

Food Chem. 2020 Nov 17;128561

Bactericidal properties of honey depend on botanical and geographical origin, where thermal treatment can have a significant affect. The aim of this study was to investigate the effect of temperature on minimum bactericidal concentration (MBC), vitamin C content, total polyphenols content and antioxidant capacity of ferric reducing antioxidant potential (FRAP) of several nectar honey varieties from northern Poland (lime, rapeseed, multifloral and buckwheat). The honeys were subjected to thermal treatment at 22 °C, 42 °C, 62 °C, 82 °C and 100 °C for two exposure times. The results showed a significant reduction of antimicrobial properties (MBC ⩾ 50%) at 82 °C and 62 °C after 15 and 120 min exposure time for most samples. Short time exposure reduced vitamin C content (⩽ 50 %) but increased total polyphenols content (⩾ 27%) and FRAP value (⩾ 106%).

Thursday, November 26, 2020

Bee Venom May Help Treat Arthritis (Osteoarthritis, Rheumatoid Arthritis, Psoriatic Arthritis, Pain, Stiffness, Swelling, Inflammation)

Investigation of anti-inflammatory effects of bee venom in experimentally induced adjuvant arthritis

Reumatologia. 2020;58(5):265-271


Objectives: Rheumatoid arthritis is a multisystemic inflammatory disease characterized by destruction of the joints. An effective treatment method of the disease has not been developed yet. The aim of the present study is to evaluate the effects of bee (Apis mellifera anatoliaca) venom (BV) on serum inflammatory parameters, serum antioxidant load and clinical parameters of experimentally induced adjuvant arthritis in rats.

Material and methods: A total of 35 Wistar albino male rats were used. The animals were divided into 5 groups. First group animals served as negative controls. The second, third, fourth and fifth groups were used for experimental arthritis induction. Following clinical development of arthritis, the first group was subcutaneously administered 0.2 ml of physiological saline, and the second, third and fourth groups were treated subcutaneously with 2 µg/kg, 4 µg/kg and 20 µg/kg once a week three times. Physiological saline injected fifth group animals were used as a sham-treatment group. Clinical observations and evaluation of arthritis were made at the 15th day, and at the end of the experiment. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, paraoxonase, serum aryl esterase, high-sensitivity C reactive protein, interleukin 1β (IL-1β), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) were determined in cardiac blood samples taken at the end of the 29th day.

Results: From the data, total oxidant level (TOL) and oxidative stress index (OSI) were calculated. Significant improvements were observed in the clinical signs of arthritis and inflammatory markers such as in IL-1β, TNF-α, IL-6 and TOL and OSI in the 20.0 µg/kg BV-administered group. Bee venom administration did not cause any significant increase in ALT and AST values or signs of liver toxicity.

Conclusions: Bee venom treatment was effective in alleviation of symptoms of the experimental rat adjuvant arthritis by means of clinical observation and serum inflammatory markers.

Tuesday, November 24, 2020

Bee Bread, Bee Pollen Show Antimicrobial Activity

Bee-collected pollen (BCP) is a well-known functional food. Honey bees process the collected pollen and store it in the hive, inside the comb cells. The processed pollen is called bee- bread or ambrosia and it is the main source of proteins, lipids, vitamins, macro-and micro-elements in honey bee nutrition. During storage, beebread undergoes solid state fermentation which preserves it and increases the bioavailability of nutrients. Research on beebread has been rather limited until now. In recent years, there is an increasing interest regarding the antimicrobial properties of BCP and beebread, due to emerging antimicrobial resistance by pathogens. Both BCP and beebread exhibit antimicrobial properties against diverse pathogens, like bacteria and fungi. As is the case with other bee products, lack of antimicrobial resistance might be attributed to the synergy of more than one antimicrobial compounds within BCP and beebread. Furthermore, BCP and bee bread exert targeted activity against pathogens and affect the host microbiome in a prebiotic manner. This review aims to present up to date research findings regarding these aspects as well as to discuss current challenges and future perspectives in the field.

...BCP or BB demonstrate selective antimicrobial activity that is usually higher against Gram-positive compared to Gram-negative bacteria. Strain specificity should be further investigated to identify BCP/BB compounds responsible for that. In that respect more studies should be conducted towards exploring synergy of BCP and BB extracts (or compounds detected within) and antibiotics. This synergy could prevent or delay microbial resistance which is a major public health issue.

Thursday, November 12, 2020

Invitation to the Online Romanian Apitherapy Congress, Nov. 20-22, 2020

The Romanian Apitherapy Society is offering next week-end (November 20-22), through ZOOM online platform, free access to our international speakers and regular participants.

For more details please check our website:

Please share this info with all your best friends and colleagues from your own national Apitherapy Associations/groups.

We have a limited number of seats in the conference room, so hurry-up with your registration.

Best regards from Romania,

Yours all,

Dr. Stefan Stangaciu

E-mail: +

Sunday, November 08, 2020

How to Make Homemade Antibiotic with Turmeric and Honey

  • 1 tablespoon of turmeric
  • 100 grams of honey (Buckwheat Honey is Great) 

In a glass jar, mix the ingredient, and stir well.

Take a tablespoon of the remedy on every hour to treat the flu. Do not swallow it immediately, but leave it to melt in the mouth. On the next day, take the same amount but on every 2 hours, and repeat on the third day, taking it only three times daily.

You can also add it to your tea or milk.

Friday, October 23, 2020

Propolis Spray Helps Treat Viral and Bacterial Upper Respiratory Tract Infections (COVID, COVID-19, Coronavirus, Pandemic)

A standardized polyphenol mixture extracted from poplar-type propolis for remission of symptoms of uncomplicated upper respiratory tract infection (URTI): A monocentric, randomized, double-blind, placebo-controlled clinical trial

Phytomedicine. 2020 Oct 8;80:153368. 


Background: The most common symptoms of mild upper respiratory tract infections (URTIs) are sore throat, muffled dysphonia, and swelling and redness of the throat, which result from the inflammation process following acute bacterial or viral infection.

Hypothesis/purpose: As propolis is a natural resinous substance traditionally used to maintain oral cavity and upper respiratory tract health due to its antimicrobial and anti-inflammatory properties, the aim of this study is to evaluate the efficacy of an oral spray based on poplar-type propolis extract with a known and standardized polyphenol content, on the remission of the symptoms associated with mild uncomplicated URTIs.

Study design: A monocentric, randomized, double-blind, placebo-controlled clinical trial was performed.

Methods: This study was conducted in 122 healthy adults who had perceived mild upper respiratory tract infections. Participants, randomly assigned to receive either propolis oral spray (N = 58) or placebo (N = 64), underwent four visits (baseline = t0, after 3 days = t1 and after 5 days = t2 and after a follow-up of 15 days = t3) in an outpatient setting. Propolis oral spray total polyphenol content was 15 mg/ml. The dosage was 2-4 sprays, corresponding to 12-24 mg of polyphenols, three times for five days. The duration of the study was 8 weeks.

Results: After 3 days of treatment, 83% of subjects treated with propolis oral spray had remission of symptoms, while 72% of subjects in the placebo group had at least one remaining symptom. After five days, all subjects had recovered from all symptoms. This means that resolution from mild uncomplicated URTIs took place two days earlier, instead of taking place in five days as recorded in the control group. There was no relationship between the ingestion of propolis oral spray or placebo and adverse reactions.

Conclusion: Propolis oral spray can be used to improve both bacterial and viral uncomplicated URTI symptoms in a smaller number of days without the use of pharmacological treatment, leading to a prompt symptom resolution.

Thursday, October 22, 2020

Italian Bee Pollens Show Antioxidant, Nutraceutical Properties (B2, B3, B6, B9, Amino Acids, Polyphenols, Flavonoids, Omega-3, Omega-6)

Antioxidant, Nutraceutical Properties, and Fluorescence Spectral Profiles of Bee Pollen Samples from Different Botanical Origins

Antioxidants (Basel), 2020 Oct 15;9(10):E1001

Bee pollen is made by honey bees (Apis Mellifera) from the pollen of plants and flowers and represents an apiary product enriched in essential amino acids, polyphenols, omega-3, and omega-6 fatty acids. 

This study investigated the botanical origin, micronutrient profile, and antioxidant activity of bee pollen samples (n = 10) harvested in Lucca and Massa Carrara (Tuscany, Italy) between 2016 and 2017. The palynological analysis showed that bee pollen samples were composed of nine botanical families. Front-face fluorescence spectroscopy was performed on bee pollen samples in bulk, without any treatment, and in ethanol extracts to determine the characteristic fluorescent profile and, to identify the main chemical compounds with biological activity. 

The main chemical compounds detected were polyphenols (mainly flavonoids and phenolic acids), hydro-soluble vitamins (B2, B3, B6, and B9), amino acids, and pigments. Furthermore, the antioxidant activity was investigated, and one of the two Viburnum pollens resulted in the highest polyphenols and flavonoids content (20.15 ± 0.15 mg GAE/g fw and 23.46 ± 0.08 mg CE/g fw, respectively). However, Prunus and Eucalyptus families showed the highest in vitro (190.27 ± 8.30 µmol Fe2+/g) and ex vivo (54.61 ± 8.51 CAA unit) antioxidant capacity, respectively. 

These results suggested that Tuscan bee pollen, depending on the botanical family, is rich in essential nutrients and potential nutraceutical product.