Friday, January 17, 2020

Manuka Honey May Help Treat Colorectal Cancer

The Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honey

Antioxidants (Basel). 2020 Jan 10;9(1)

Manuka honey (MH) is a natural food with many beneficial properties to human health, thanks to its high variety of bioactive compounds; however, little is known about its bioaccessibility.

The aim of this study was to evaluate and compare the polyphenol compounds, the antioxidant capacity and the anticancer activity of MH subjected to an in vitro gastrointestinal digestion in human HCT-116 colon cancer cells.

Raw MH and digested MH (DMH) were assessed for total polyphenols and flavonoids by spectrophotometric and HPLC-ESI-MS/MS analysis, and total antioxidant capacity (TAC) using different methods. Cell viability, intracellular ROS production, apoptosis, cell cycle and colony formation capacity were tested after treatment with MH or DMH.

Results showed that total polyphenols, total flavonoids and TAC were significantly (p < 0.05) reduced after in vitro digestion. In addition, MH and DMH at 8, 16 and 24 mg/mL had similar effects in inducing intracellular ROS production and in inhibiting the colon formation ability; MH induced a more marked apoptosis compared to DMH, while cell cycle was blocked in S phase by MH and in Sub G1 phase by DMH.

Our results increase knowledge of the effect of gastrointestinal digestion on the biological effect of honey against colorectal cancer.

Thursday, January 09, 2020

Honey Protects Against Toxic Agents


Antidotal or protective effects of honey and one of its major polyphenols, chrysin, against natural and chemical toxicities

Acta Biomed. 2019 Dec 23;90(4):533-550

OBJECTIVE:

Honey and its polyphenolic compounds are of main natural antioxidants that have been used in traditional medicine. The aim of this review was to identify the protective effects of honey and chrysin (a polyphenol available in honey) against the chemical and natural toxic agents.

METHOD:

The scientific databases such as MEDLINE, PubMed, Scopus, Web of Science and Google Scholar were searched to identify studies on the antidotal effects of honey and chrysin against toxic agents.

RESULTS:

This study found that honey had protective activity against toxic agents-induced organ damages by modulating oxidative stress, inflammation, and apoptosis pathways. However, clinical trial studies are needed to confirm the efficacy of honey and chrysin as antidote agents in human intoxication.

CONCLUSION:

Honey and chrysin may be effective against toxic agents. (www.actabiomedica.it).

Thursday, January 02, 2020

Bee Pollen Shows Gut-Protecting Potential


Lipidomics Provides Novel Insights into Understanding the Bee Pollen Lipids Transepithelial Transport and Metabolism in Human Intestinal Cells

J Agric Food Chem. 2019 Dec 26

Bee pollen (BP) shows profound gut-protecting potentials. BP lipids (BPLs) mainly composed by phospholipids and polyunsaturated fatty acids might be one of the important contributors, while how BPL exerts gut-protecting effects and is transported through intestinal cell monolayers need to be investigated.

Here, we exploited a strategy that combines an UPLC-Q-exactive orbitrap/MS-based lipidomics approach with a human intestinal cell (Caco-2) monolayer transport model, to determine the transepithelial transportation of BPL from Camellia sinensis L. (BPL-Cs), in pathological conditions.

The results showed that BPL-Cs protected Caco-2 cells against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction by improving cell viability, maintaining membrane integrity, increasing tight junctions (ZO-1 and Claudin-1), and eliciting the expressions of antioxidative-related genes (NQO1, Nrf2, Txnrd1, and GSTA1). Lipidomics analysis revealed that DSS suppressed the transport and uptake of most of BPL-Cs including glycerophospholipids, sphingomyelins, and glycosylsphingolipids. Pretreatment with BPL-Cs significantly regulated glycerophospholipid and sphingolipid metabolisms, potentially involved in building permeability barriers and alleviating intestinal oxidative stress.

Finally, eight classes of lipids were identified as the potential biomarkers for evaluating DSS-induced Caco-2 cell dysfunctions and BPL-intervened modulation.

These findings shed light on the development of BPL as gastrointestinal protective food supplements in the future.

Friday, December 27, 2019

Royal Jelly Helps in Cognitive Support, Healing Wounds, Fertility Support, Antioxidant and Anti-Inflammatory Effects, Kidney and Liver Disease Support, Healthy Skin, Energy and Vitality, Digestive Support, Bone Support, Diabetic Support and Protection Against Neurodegenerative Diseases


Royal Jelly Health Benefits: Improves Collagen Levels And Mor

Dec 27, 2019

If you have no idea what royal jelly is, then it might sound like something either prestigious or expensive, sitting atop shelves out of reach of children. After all, it does say ‘royal’ in the name. But if you’re already familiar with what it does, then that opinion might not change at all since unsurprisingly so, royal jelly does deserve its name.

Why? Simple, because it’s been reported to have anti-diabetic, antioxidant, antibacterial and wound healing properties. And much like honey, it can be eaten too, with the benefit including asthma natural remedy, age-defying treatment, healthy bone development and to boost our immune system, and that’s not even all of it.

Here’s what you need to know about this amazing natural substance.

Royal Jelly

Similar to honey, royal jelly (RJ) is also produced by bees, although this nutritious substance is only produced by young nurse ones from the hive. As a substance, RJ contains a large amount of amino acids, which we can use for both reproduction and healthy cell growth. Bees usually use it for the nutrition of adult queen bees and her larvae. It's the sole food that queen and nurse bees eat all their lives, as well as what larvae eat for three days after hatching. In total, it’s the main food of bees, while honey is used to provide them energy...

Saturday, December 21, 2019

Honey Boosts Effectiveness of Traditional Medicine


Natural deep eutectic characteristics of honey improve the bioactivity and safety of traditional medicines

J Ethnopharmacol. 2019 Dec 11:112460

ETHNOPHARMACOLOGICAL RELEVANCE:

Honey, an important additive with natural deep eutectic solvent (NADES) characteristics, has been used in traditional medicine for thousands of years.

AIM OF THE STUDY:

We investigated the quality-improving effects of honey on Astragali Radix (Mikvetch Root) (RA) as an example.

MATERIALS AND METHODS:

Decoctions of raw RA, fried RA, honey-fried RA, and a man-made- honey-fried RA were prepared and compared in cell-based bioactivity tests, chemical composition tests, as well as a bioavailability test with calycosin-7-O-β-D-glucoside.

RESULTS:

The addition of honey increased the concentrations of active compounds and their oral bioavailability, provided protection against acetylation, and consequently increased their bioactivity. These changes were also observed when a pure NADES-mimicking honey was used.

CONCLUSION:

Our findings provide a potential explanation as to why honey has long been used as traditional medicine additives and rationalize the application of honey and honey-like substance in producing pharmaceuticals.

Wednesday, December 18, 2019

Propolis-Enriched Stingless Bee Honey Successfully Treats Diabetic Foot Ulcer with Exposed Bone

Successful treatment of a diabetic foot ulcer with exposed bone using Trigona honey: a case study

J Wound Care. 2019 Dec 1;28(Sup12):S4-S8

Many studies have shown that honey might improve wound healing. However, its efficacy for large wounds which may be followed by a systemic effect remains unclear. The effectiveness of honey-based dressings in treating large diabetic foot ulcers (DFU) is still unknown.

This study presents the case of a 38-year-old female patient presenting with an extensive infected DFU with exposed bone. The DFU was treated with propolis-enriched Trigona honey, used as a single treatment, in a home visit setting. After two months' follow-up, the wound exhibited complete re-epithelialisation despite the patient's initial poor condition.

Tuesday, December 10, 2019

Manuka Honey Used to Fight Infections in Surgical Dressings


Potential of Manuka Honey as a Natural Polyelectrolyte to Develop Biomimetic Nanostructured Meshes With Antimicrobial Properties

Front. Bioeng. Biotechnol., 04 December 2019

The use of antibiotics has been the cornerstone to prevent bacterial infections; however, the emergency of antibiotic-resistant bacteria is still an open challenge.

This work aimed to develop a delivery system for treating soft tissue infections for: (1) reducing the released antimicrobial amount, preventing drug-related systemic side effects; (2) rediscovering the beneficial effects of naturally derived agents; and (3) preserving the substrate functional properties. For the first time, Manuka honey (MH) was proposed as polyelectrolyte within the layer-by-layer assembly. Biomimetic electrospun poly(ε-caprolactone) meshes were treated via layer-by-layer assembly to obtain a multilayered nanocoating, consisting of MH as polyanion and poly-(allylamine-hydrochloride) as polycation. Physicochemical characterization demonstrated the successful nanocoating formation.

Different cell lines (human immortalized and primary skin fibroblasts, and primary endothelial cells) confirmed positively the membranes cytocompatibility, while bacterial tests using Gram-negative and Gram-positive bacteria demonstrated that the antimicrobial MH activity was dependent on the concentration used and strains tested.

Monday, December 09, 2019

How Manuka Honey Can Improve Your Digestion, Heal Wounds, Promote Oral Hygiene, Treat Colds and Coughs


How Manuka honey can help improve your health, especially during winters

Manuka honey is native to Australia and New Zealand. It has many benefits and uses like healing wounds, aiding digestion, and promoting oral hygiene, among others.

KEY HIGHLIGHTS

  • Honey is used all around the world as a popular condiment in food and home remedies
  • Manuka honey is produced in Australia and New Zealand
  • Here is how Manuka honey can be used to avail its many health benefits

New Delhi: Honey, as everybody knows, is a healthy food condiment. Honey has many health benefits and uses. It is used in weight loss remedies, as a natural source of sugar, to treat infections, and even as skincare solutions. Honey is of different types, based on how it is prepared and where it comes from. One such type of honey is the Manuka Honey.

Manuka honey is made in the southern continent of Australia, and its neighbouring island, New Zealand. The honey is prepared by bees that pollinate the Manuka bush, native to the region. Manuka honey is used widely and has many health benefits....

Thursday, December 05, 2019

People are Trying Bee Stings to Treat Lyme Disease

Katy Vine shares what she learned writing a story about people using bee stings to treat lyme disease.

Wednesday, December 04, 2019 7:42 a.m. by Amy Iler

In this podcast: Katy Vine,  Executive Editor of Texas Monthly, joins It Takes 2 to talk about a fascinating story she wrote about people desperately seeking treatments for lyme disease.


Thursday, November 28, 2019

Indonesian Stingless Bee Propolis is a Potential Non-Steroidal Anti-Inflammatory Drug


Anti-inflammatory activity of Tetragronula species from Indonesia

Saudi J Biol Sci. 2019 Nov;26(7):1531-153

Anti-inflammatory drugs inhibit inflammation, particularly those classified as nonsteroidal anti-inflammatory drugs (NSAIDs). Several studies have reported that propolis has both anti-ulcerogenic and anti-inflammatory effects.

In this study, we investigated the bioactive compound and in vivo anti-inflammatory properties of both smooth and rough propolis from Tetragronula sp. To further identify anti-inflammatory markers in propolis, LC-MS/MS was used, and results were analyzed by Mass Lynx 4.1. Rough and smooth propolis of Tetragonula sp. were microcapsulated with maltodextrin and arabic gum. Propolis microcapsules at dose 25-200 mg/kg were applied for carrageenan-induced rat's paw-inflammation model.

Data were analyzed by one-way ANOVA and Kruskal-Wallis statistical tests. LC-MS/MS experiments identified seven anti-inflammatory compounds, including [6]-dehydrogingerdione, alpha-tocopherol succinate, adhyperforin, 6-epiangustifolin, deoxypodophyllotoxin, kurarinone, and xanthoxyletin. Our results indicated that smooth propolis at 50 mg/kg inhibited inflammation to the greatest extent, followed by rough propolis at a dose of 25 mg/kg. SPM and RPM with the dose of 25 mg/kg had inflammatory inhibition value of 62.24% and 58.12%, respectively, which is comparable with the value 70.26% of sodium diclofenac with the dose of 135 mg/kg.

This study suggests that propolis has the potential candidate to develop as a non-steroid anti-inflammatory drug.

Friday, November 22, 2019

What is Apitherapy?


New Bee-Centric Spa Has Us Buzzing

Worker B Wellness offers holistic services using bee-derived products and provides free educational sessions about bees

Minnesota Monthly

Apitherapy is a form of alternative medicine that uses honey bee products such as honey, propolis, pollen, royal jelly, and bee venom. This type of therapy has existed for thousands of years. According to The American Apitherapy Society Inc., rock art created in ancient times illustrated that early hunter-gatherers viewed honeybees as a source of natural medicine. In fact, apitherapy was used to treat arthritis and other joint problems, which is still practiced today.

Romanelli has always had an interest in bees. She laughs as she says her family was “urban hippies”—always growing fresh produce and eating organic, local foods.

“My interest in bees is really an interest in the product they provide and also how they work in community with each other, which is really fascinating,” Romanelli says. “Each bee has a specific job in the hive to help the whole as a superorganism. So, when we were looking at this, we’re like, ‘OK, so Worker B Wellness is a superorganism—we’re all doing something to help the concept of the hive.”...

Thursday, November 21, 2019

Can Bee Stings Treat Lyme Disease?


Treatments for chronic Lyme disease are controversial and expensive. As a last resort, some patients are pursuing this unproven and painful alternative.

TEXAS MONTHLY, DECEMBER 2019

Avery was 41 and had been diagnosed with Lyme in 2013, though her symptoms dated back years before that. She told me that it had taken a few months to work up to ten stings; she thought the bee venom was setting off something called a Jarisch-Herxheimer reaction, a response to antimicrobials like antibiotics, in which the bacteria being destroyed release their contents into the bloodstream and cause flu-like symptoms. (It’s not scientifically documented whether venom in fact triggers the same reaction.) “I’m starting to handle it better than at first,” Avery said. “Like I’m not—knock on wood—getting as sick now because the bacterial load is going down, and I think I have a pretty good detox routine.” On the day following a stinging session, the women typically soak in Epsom salt baths, dry brush the skin around their lymph glands, and take coffee enemas—an effort they believe will expel toxins.

Like a nurse giving a vaccine, Gschwind held a bee in tweezers as Avery removed her ice pack. “Okay, ready?” Gschwind asked. “One, two, three.” She held the bee’s butt about an inch to the left of Avery’s lower spine and waited for the tiny stinger to pierce the skin. Then she pulled away the bee, now missing its stinger, which was still drilling into Avery’s back. The vibrating fuzzball was doomed without its barb, and Gschwind placed it in some soapy water to hasten its inevitable end. Afterward, Avery grimaced and breathed deeply as she returned the ice pack to her lower spine, preparing for the next sting.

This process would continue until the bottom portion of Avery’s spine looked like a clear highway running between two parallel rows of five inflamed bumps. Each protrusion was dotted with a stinger, moving like a pumpjack atop a tiny, rising hill, as it continued to gently, rhythmically, excrete venom. Twenty minutes later, when the barbs had done their job and stopped moving, Gschwind extracted them. Then Avery went through the same procedure to sting Gschwind.

While everything about this routine was taxing—the stinging, the seeming Herxheimer reaction, collecting bees and keeping them in her apartment—Gschwind didn’t see much of an alternative. She could not sit on the couch and groan in pain year in and year out while doctors told her she was fine, or that she should be fine, or that she would feel better in time. She’d tried that already. “What am I going to do? Because this isn’t fair. I deserve to have a life, to be functional,” she said. “Well, I guess I’m going to stick myself with bees.”...

Monday, November 18, 2019

Black Seed Oil, Honey, Whey Protein Increase Antioxidant Activity


Effect of black seed oil, honey, whey protein concentrate and their interaction on antioxidant activity, elastic modulus and creaming index of O/W emulsions

Journal of Dispersion Science and Technology

An emulsion based on a combination of highly-valued black seed oil (BSO) and honey shall be introduced as an emerging nutraceutical since the emulsion could be an efficient carrier for bioactive compounds from both.

This study aimed to determine effect of BSO, honey, whey protein concentrate (WPC) and their interaction on antioxidant activity (i.e. IC50, the concentration of sample required to scavenge 50% of 2,2-diphenyl-1-picrylhydrazyl free radicals), elastic modulus (G′) and creaming index (CI) of O/W emulsions by means of a response surface methodology. Twenty emulsions were ultrasonically prepared by using various combinations of BSO (10–20%), honey (10–20% and WPC (2–6%) based on a central composite design. Regression analysis (R2 = 0.92–1.00) revealed that a decrease in the IC50 was mainly due to significant (p < 0.05) linear effect of honey and WPC.

The quadratic effect of WPC significantly increased the G′ yet decreased the CI. Synergistic effects of BSO-honey on IC50 and G′ were also significant (p < 0.05). However, the antagonistic effect (p < 0.05) of honey-WPC seemed to increase the IC50. By using the fitted quadratic models, the optimized levels of BSO (20.0%), honey (18.2%) and WPC (6.0%) were proposed and predicted to provide the desired emulsion with IC50 = 0.12 mg/ml, G′ = 606.65 Pa and CI = 1.45%. These values were successfully validated with their respective experimental values.

Monday, November 11, 2019

Propolis an Eco-Friendly Antibacterial Coating for Wound Sutures

Characterization of silk sutures coated with propolis and biogenic silver nanoparticles (AgNPs); an eco-friendly solution with wound healing potential against surgical site infections (SSIs)

Turk J Med Sci. 2019 Oct 27

BACKGROUND/AIM:

Bacterial adherence to a suture material is one of the main reasons that cause surgical site infections. An antibacterial suture material with enhanced wound healing function may prevent the surgical site from infections. Thus, the present study was aimed to investigate the synergistic effect of propolis and biogenic metallic nanoparticles when combined with silk sutures for biomedical use.

MATERIALS AND METHODS:

Silver nanoparticle (AgNP) synthesis was carried out via a microbial-mediated biological route and impregnated on propolis-loaded silk sutures using an in situ process. silk sutures fabricated with propolis and biosynthesized AgNPs (bioAgNP-propolis coated sutures) were intensively characterized using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC). Antibacterial characteristics of the bioAgNP-propolis coated sutures was evaluated using agar plate method. Biocompatibility of the bioAgNP-propolis coated sutures was evaluated using 3T3 fibroblast cells and their wound healing potential was also investigated.
RESULTS:

BioAgNP-propolis coated sutures displayed potent antibacterial activity against pathogenic Gram negative and Gram positive bacteria; Escherichia coli and Staphylococcus aureus, respectively. BioAgNP-propolis coated silk sutures were found to be biocompatible with 3T3 fibroblast cell culture. In vitro wound healing scratch assay was also demonstrated that the extract of bioAgNP-propolis coated sutures stimulated the 3T3 fibroblasts? cell proliferation.

CONCLUSION:

Coating the silk sutures with propolis and biogenic AgNPs gained an effective antibacterial capacity to surgical sutures besides providing biocompatibility and wound healing activity.

Friday, November 08, 2019

Honey and Its Combination with Metformin Prevents Hyperglycemia, Stimulates Insulin Secretion, Reduces Liver Fat Accumulation, Attenuates Liver Injury and Kidney Damage


Combination of honey with metformin enhances glucose metabolism and ameliorates hepatic and nephritic dysfunction in STZ-induced diabetic mice

Food Funct. 2019 Nov 5

Honey is a natural sweetener that contains a large amount of monosaccharides such as glucose and fructose, as well as small amounts of disaccharides and trisaccharides such as sucrose and pine trisaccharides. In addition to carbohydrates, honey also contains vitamins, minerals, enzymes, amino acids, and polyphenols including phenolic acids and flavonoids.

The polyphenols in honey have been proved to have great antioxidant activity, besides inhibiting α-glycosidase activity and improving blood-lipid metabolism. However, whether it is safe for diabetic patients to consume honey remains controversial.

This study investigated the effects of honey, metformin and their combination on the characteristic pathological changes and glucose metabolism in STZ-induced diabetic mice over five weeks.

Our results showed that honey and its combination with metformin could prevent hyperglycemia, stimulate insulin secretion, reduce liver fat accumulation, attenuate liver injury and kidney damage in STZ-induced diabetic mice.

Moreover, treatment with honey or combination of honey and metformin significantly enhanced glucokinase (GK) activity (p < 0.05), and meanwhile suppressed the activities of glucose-6-phosphatase (G6Pase), phosphoenolpyruvate carboxykinase (PEPCK), pyruvate carboxylase (PC) and pyruvate dehydrogenase kinases (PDK) (p < 0.05) in diabetic mice.