Monday, October 16, 2017

Lip Balm Containing Propolis Better Than Aciclovir Cream in Treating Cold Sores

Comparative Study with a Lip Balm Containing Propolis Special Extract GH 2002 0,5 % vs. Aciclovir Cream 5 % in Patients with Herpes labialis in the Papular / Erythematous stage. A Single-blind, Randomized Two-arm Study

Current Therapeutic Research

Available online 14 October 2017

Background and Objectives

This controlled single-blind trial compared the efficacy of a lip balm with propolis special extract GH 2002 at a concentration of 0.5 % in the treatment of episodes of Herpes labialis with that of aciclovir 5% cream.

Patients and Methods

Patients in the erythematous/papular stage were randomized to two groups: 189 patients were treated with propolis cream, 190 patients with aciclovir (ITT population). Application was five times daily. The primary parameter was the difference in median time to complete encrustation or epithelization of lesions. Secondary parameters were the development of typical Herpes symptoms (pain, burning and itching, tension and swelling), the global assessment of efficacy, and the safety of application.


The predefined clinical situation was reached after a median of four days with propolis and after five days with aciclovir (p < 0.0001). Significant differences in favor of the study preparation were also found with all secondary parameters and symptoms. No allergic reactions, local irritations or other adverse events were observed.


Propolis GH 2002 extract lip balm 0.5 % was found superior in the treatment of episodes of Herpes labialis over aciclovir cream 5 % in patients in the papular/erythematous phase upon inclusion.

Sunday, October 15, 2017

Pollen Diversity of Bee Bread Is Linked to Its Nutrient Content

Nutritional composition of honey bee food stores vary with floral composition

Oecologia, First Online: 14 October 2017

Sufficiently diverse and abundant resources are essential for generalist consumers, and form an important part of a suite of conservation strategies for pollinators. Honey bees are generalist foragers and are dependent on diverse forage to adequately meet their nutritional needs. Through analysis of stored pollen (bee bread) samples obtained from 26 honey bee (Apis mellifera L.) hives across NW-England, we quantified bee bread nutritional content and the plant species that produced these stores from pollen.

Protein was the most abundant nutrient by mass (63%), followed by carbohydrates (26%). Protein and lipid content (but not carbohydrate) contributed significantly to ordinations of floral diversity, linking dietary quality with forage composition. DNA sequencing of the ITS2 region of the nuclear ribosomal DNA gene identified pollen from 89 distinct plant genera, with each bee bread sample containing between 6 and 35 pollen types. Dominant genera included dandelion (Taraxacum), which was positively correlated with bee bread protein content, and cherry (Prunus), which was negatively correlated with the amount of protein. In addition, proportions of amino acids (e.g. histidine and valine) varied as a function of floral species composition.

These results also quantify the effects of individual plant genera on the nutrition of honey bees. We conclude that pollens of different plants act synergistically to influence host nutrition; the pollen diversity of bee bread is linked to its nutrient content. Diverse environments compensate for the loss of individual forage plants, and diversity loss may, therefore, destabilize consumer communities due to restricted access to alternative resources.

Saturday, October 14, 2017

VIDEO: Gerard Butler Almost Had A Fatal Overdose...Of Bee Venom

By James McClure  |  Oct 13, 2017 

Gerard Butler is lucky to be alive after nearly suffering a fatal overdose...of bee venom. No, the Scottish actor wasn't partying too hard near an apiary when he decided to shoot up with stingers. He turned to venom to cope with the strain of working on the set of 'Geostorm,' which opens next weekend.

"I had a lot of inflammation," Butler told Seth Meyers yesterday. "I was hanging a lot on wires in this 65 pound space suit, and that's fine for a couple hours, but after five weeks, you're hurting."

So he turned to his nutritionist, who recommended taking bee venom, which supposedly has anti-inflammatory compounds that could've helped Butler battle through the gruelling shoot if it'd been injected properly. But the bee-venom dealer that he flew in wasn't very good at his job. And yes, Butler realizes how sketchy this whole situation sounds in retrospect...

Friday, October 13, 2017

Beeswax Helps Treat Burns

The effect of a beeswax, olive oil and Alkanna tinctoria (L.) Tausch mixture on burn injuries: An experimental study with a control group

Complement Ther Med. 2017 Oct;34:66-73


This study was planned to investigate the effect of a mixture of beeswax, olive oil and A. Tinctoria (L.) Tausch on burn wounds to determine the impact on burn healing, pain during dressing changes and duration of hospital stay.


The study was conducted between May 2014 and August 2015 in the Burn Unit of Ataturk University Research Hospital. The sample of this experimental study consisted of 64 patients (31 experimental group and 33 control group) who met its inclusion criteria. While the specially prepared dressing material was applied to the experimental group, the control group was administered the clinic's routine dressing. The injuries were photographed before each dressing. Each picture was uploaded to a computer for measurement with ImageJ software...

When a beeswax, olive oil and A. tinctoria (L.) Tausch mixture was applied to second degree burns, this accelerated epithelization, reduced the pain experienced during dressing changes and shortened the hospital stay durations of the patients.

Thursday, October 12, 2017

Honey Helps Treat Contact Lens Induced Dry Eye

Treatment of contact lens related dry eye with antibacterial honey

Contact Lens and Anterior Eye

Available online 9 October 2017


Contact lens induced dry eye affects approximately 50% of contact lens wearers. The aim was to assess the effects of Manuka (Leptospermum sp.) honey eye drops (Optimel, Melcare, Australia) on dry eye in contact lens wearers. The safety of the honey eye drops in contact lens wear and contact lens wearers’ compliance were also evaluated.


Prospective, randomised, cross over study, examiner masked, pilot treatment trial.


Twenty-four participants aged 20 to 55 years with contact lens related dry eye were recruited and randomised to two treatment groups; 20 completed the study. One group used Optimel eye drops twice a day for two weeks followed by conventional lubricant (Systane Ultra, Alcon) therapy for two weeks; the other group completed the treatments in the reverse order. Before and after each treatment dry eye symptomology, ocular surface inflammation, and tear quantity and quality were assessed. Participants completed a daily log detailing their usage of treatments and any issues.


Dry eye symptoms improved significantly after Optimel treatment. Patients with more severe symptoms at baseline showed a greater improvement in symptoms. No significant differences were observed in the objective signs of dry eye; presumably because of the short treatment duration. Seventy-five% of contact lens wearers reported good adherence to Optimel treatment and 95% reported no issues using this product.


Optimel Eye Drops reduce the symptoms of dry eye in contact lens wearers and are safe to use. A longer treatment period to assess the effect on clinical signs of dry eye is required.

Wednesday, October 11, 2017

Propolis May Help Cure nail Fungus

Antibiofilm activity of propolis extract on Fusarium species from onychomycosis

Future Microbiol. 2017 Oct 4


The present study evaluated the capacity of three species of Fusarium isolated from onychomycosis to form biofilms and the antibiofilm effect of propolis extract on these biofilms.


The biofilms and antibiofilm effects were evaluated by quantifying the colony-forming units, mitochondrial metabolic activity assays, total biomass by crystal violet staining and scanning electron microscopy.


Propolis extract demonstrated significant antibiofilm efficiency on Fusarium spp. isolates and reduced F. solani, F. oxysporum and F. subglutinans mature biofilms.


Propolis extract can be an alternative topical treatment of onychomycosis caused by Fusarium spp.

Tuesday, October 10, 2017

Propolis Boosts Immunity

Propolis supplementation attenuates the negative effects of oxidative stress induced by paraquat injection on productive performance and immune function in turkey poults

Poultry Science
Published: 05 October 2017 

Paraquat (PQ) is used as a herbicide in agriculture and causes oxidative and inflammatory damage to animal tissues. The current study was conducted to investigate the positive effects of dietary propolis (PR), as a potent naturally produced antioxidant, on growth performance and immune function of turkey poults exposed to oxidative stress induced by PQ injection.

Native male turkey poults (n = 120, 49-d-old) were randomly assigned into 4 groups: poults received a basal diet with a daily subcutaneous PQ injection of 5 mg/kg BW for 7 consecutive days (PQ group), an experimental diet containing 1 g/kg PR with a daily subcutaneous PQ injection for 7 days (PR+PQ group), or received the experimental PR diet with a daily subcutaneous injection of 0.5 mL sterile saline for 7 days (PR group); while the control poults received a basal diet with a daily subcutaneous saline injection for 7 consecutive days (C group). The productive performance in the PQ group showed a significant (P < 0.05) reduction in the weight gain (WG) and feed intake (FI), and impaired feed conversion ratio (FCR). Propolis supplementation in the PR+PQ group significantly ameliorated the PQ effects on WG and FCR. Turkey poults of the PQ and PR+PQ groups had a significant augmentation in the blood malondialdehyde (MDA), tumor necrosis factor-α (TNFα), and corticosterone levels, and in contrast, a significant reduction in the triiodothyronine (T3), when compared to the C group.

While propolis significantly reduced the MDA and corticosterone, and increased the T3 levels in the PR+PQ group compared to the PQ group. Furthermore, the dietary PR supplementation significantly limited the PQ-suppressive effects on cell- and humoral-mediated immunity and lymphocyte proliferation of turkey poults. In addition, propolis supplementation in the PR and PR+PQ groups markedly reversed the PQ-induced DNA fragmentation and heat shock protein 70 (Hsp70) over-expression in blood cells.

It can be concluded that PR could improve turkey immunity and performance, particularly under inflammation and oxidative stress induced by PQ exposure.

Monday, October 09, 2017

Manuka Honey May Be Effective in Treating Atopic Dermatitis Lesions

Manuka honey tested as AD treatment
Pilot study shows evidence of healing in atopic dermatitis

Dermatology Times, October 06, 2017

An open-label, pilot study has found that the topical application of manuka honey may be effective in treating atopic dermatitis lesions.

Abdullah A. Alangari M.D., the study’s lead author and an allergist at King Saud University in Saudi Arabia, says he was prompted to investigate the impact of honey after receiving anecdotal reports from patients saying they benefited from using honey to treat atopic dermatitis lesions. The study appears in a recent issue of Immunity, Inflammation and Disease.

Honey is well-recognized for its medicinal qualities and is currently being employed in wound care and as a burn treatment. Other studies have shown that it can provide antibiotic resistance to specific strains of bacteria. It's health properties are continually under study throughout the world.

In this study, investigators recruited 16 individuals (8 female, mean age 33), but two withdrew due to worsening symptoms. “We asked patients with bilateral atopic dermatitis lesions to place honey topically on one site overnight and leave the contralateral site untreated. We noted that on average the lesions treated with honey improved more than lesions that were untreated,” he said...

Sunday, October 08, 2017

Is the buzz around honey water here to stay?

Verdict, 10/6/2017

The latest addition to the list of wonder ingredients — honey — is being used for health, beauty, and even sport and as an alternative to refined sugar.

In the Venn diagram of health, beauty and sports trends, honey is firmly in the centre as a powerhouse of an ingredient.

Saturday, October 07, 2017

Royal Jelly May Help Treat Milk Allergy

Preventive effects of royal jelly against anaphylactic response in a murine model of cow's milk allergy

Pharm Biol. 2017 Dec;55(1):2145-2152


Royal jelly (RJ) has long been used to promote human health.


The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow's milk proteins.


Balb/c mice treated orally for seven days with RJ at doses of 0.5, 1 and 1.5 g/kg were sensitized intraperitoneally with β-lactoglobulin (β-Lg). Serum IgG and IgE anti-β-Lg were determined by an enzyme-linked immunosorbent assay (ELISA). Plasma histamine levels, symptom scores and body temperature were determined after in vivo challenge to β-Lg. Jejunums were used for assessment of local anaphylactic responses by an ex vivo study in Ussing chambers and morphologic changes by histological analysis.


RJ significantly decreased serum IgG (31.15-43.78%) and IgE (64.28-66.6%) anti-β-Lg and effectively reduced plasma histamine level (66.62-67.36%) (p < 0.001) at all the doses tested. Additionally, no clinical symptoms or body temperature drops were observed in RJ-pretreated mice. Interestingly, RJ significantly reduced (p < 0.001) intestinal dysfunction by abolishing the secretory response (70.73-72.23%) induced by sensitization and prevented length aberrations of jejunal villi by 44.32-59.01% (p < 0.001).


We speculate that using RJ may help prevent systemic and anaphylactic response in allergic mice. These effects may be related to its inhibitory effects on the degranulation of mast cells.

Friday, October 06, 2017

Honey Better than Phenytoin for Healing Episiotomy Wounds

Comparison of honey and phenytoin (PHT) cream effects on intensity of pain and episiotomy wound healing in nulliparous women

J Complement Integr Med. 2017 Oct 5


This study investigates and compares the effect of phenytoin and honey cream on intensity of pain and episiotomy wound healing in nulliparous women.


This research was conducted by double-blinded randomized clinical trial method on 120 nulliparous women in three groups of phenytoin, honey and placebo. After delivery and episiotomy, mothers used a knuckle of the prescribed creams on their episiotomy area, every night until 10-night. Evaluation of wound healing using REEDA scale and pain intensity with numerical rating scale of pain took place within the first 24 h and after 7 and 14 days of delivery.


Results showed significant differences between healing scores of the three groups for the seventh day after delivery. Healing score in the honey group was lower than that in placebo group. On the fourteenth day after delivery, the healing scores showed no significant differences between the three groups. Measuring pain intensity did not show any significant difference in the three groups after 7 and 14 days of delivery.


Both honey and phenytoin led to episiotomy wound healing; however, honey caused the wound healing better than the phenytoin. Honey and phenytoin did not reduce episiotomy pain.

Thursday, October 05, 2017

Methicillin Resistant Staphylococcusaureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) Isolates Sensitive to Honey

Antimicrobial Activity of Honey with Special Reference to Methicillin Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA)

J Clin Diagn Res. 2017 Aug;11(8):DC05-DC08


Antimicrobial agents are essentially important in reducing the global burden of infectious diseases. With the irrational and excessive use of antibiotics in underdeveloped and developing countries, there may be chances to develop and spread resistant pathogens in the community. As a result, the effectiveness of the antibiotics is diminishing. Therefore, the need for novel alternative antimicrobial strategies has renewed interest in natural products like turmeric, honey, ginger and others exhibiting antibacterial properties. This situation has led to a re-evaluation of the therapeutic use of ancient remedies like honey as no other studies are available in the state of Andhra Pradesh with the locally available honey.


To find out the efficacy of antibacterial activity of locally available honey against Methicillin Resistant Staphylococcusaureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) isolates.


A prospective study on the antibacterial activity of Bharat multi floral pasteurised honey which was locally available in the state of Andhra Pradesh, further it was conducted and evaluated against the bacterial strains of Methicillin Resistant Staphylococcus aureus and Methicillin Sensitive Staphylococcus aureus. Their antibacterial sensitivity pattern was tested using Kirby-Bauer disc diffusion susceptibility testing technique of CLSI along with other commonly used antimicrobials.


Both MRSA and MSSA isolates were sensitive to honey. But MRSA were resistant to all antimicrobials tested except linezolid where as MSSA were sensitive to all except penicillin.


It is definitely worthy to consider honey as a promising future antimicrobial to be tested and studied. Honey, may be elaborately used in future with some more molecular studies on its method of action as an antimicrobial agent.

Wednesday, October 04, 2017

Evaluation of bioactive compounds potential and antioxidant activity of brown, green and red propolis from Brazilian northeast region

Food Res Int. 2017 Nov;101:129-138

The aim of the present study was to determine the contents of bioactive compounds present in brown, green and red species of propolis cultivated in the Brazilian northeast states of Alagoas and Sergipe. The contents of phenolic compounds, flavonoids and antioxidant activity (DPPH, ABTS+, FRAP, ORAC) were determined. Identification and quantification of phenolic and flavonoid compounds were performed by using UHPLC-QqQ-MS/MS system. The results revealed high contents of total phenolics and flavonoids. Among the three species, the antioxidant potential had higher capacity in the red propolis. The presence of some of bioactive compounds viz. acacetin, artepellin C, eriodictyol, gallic acid, isorhamnetin, protocatechuic acid, vanillin and vanillic acid in Brazilian red propolis is reported for the first time in this work. Positive correlation between total phenolics versus the FRAP and ORAC methods was established which led to conclusion that antioxidant activity of propolis is mainly due to its phenolic compounds.

Tuesday, October 03, 2017

Propolis Component May Help Treat Oral Cancer

Apoptosis induced by caffeic acid phenethyl ester in human oral cancer cell lines: Involvement of Puma and Bax activation

Arch Oral Biol. 2017 Sep 25;84:94-99


Caffeic acid phenethyl ester (CAPE), a natural honeybee product exhibits a spectrum of biological activities including antimicrobial, anti-inflammatory, antioxidant and antitumor actions. The purpose of this research was to investigate the anticancer potential of CAPE and its molecular mechanism in human oral cancer cell lines (YD15, HSC-4 and HN22 cells).


To determine the apoptotic activity of CAPE and identify its molecular targets, trypan blue exclusion assay, soft agar assay, Western blot analysis, DAPI staining, and live/dead assay were performed.


CAPE significantly suppressed transformation of neoplastic cells induced by epidermal growth factor (EGF) and 12-O-tetradecanoylphorbol 13-acetate (TPA) without inhibiting growth. CAPE treatment inhibited cell growth, increased the cleavages of caspase-3 and poly (ADP-ribose) polymerase (PARP), and augmented the number of fragmented nuclei in human oral cancer cell lines. CAPE activated Bax protein causing it to undergo a conformational change, translocate to the mitochondrial outer membrane, and oligomere. CAPE also significantly increased Puma expression and interestingly Puma and Bax were co-localized.


Overall, these results suggest that CAPE is a potent apoptosis-inducing agent in human oral cancer cell lines. Its action is accompanied by up-regulation of Bax and Puma proteins.

Monday, October 02, 2017

Royal Jelly Slows Rate of Muscle Strength Decrease in the Elderly

Effects of protease-treated royal jelly on muscle strength in elderly nursing home residents: A randomized, double-blind, placebo-controlled, dose-response study

Sci Rep. 2017 Sep 12;7(1):11416

Although we have found that protease-treated royal jelly (pRJ) benefit for the skeletal muscle mass and strength in the aged animals, the potential beneficial effects have not been evaluated in humans.

The aim of this study was to determine whether pRJ intake had beneficial effects on muscle strength in elderly nursing home residents. One hundred and ninety-four subjects enrolled into this multicenter, randomized, double-blind, placebo-controlled study. Subjects received either placebo(Group 1), pRJ 1.2 g/d(Group 2), or 4.8 g/d(Group 3). Data through 1 year are reported for 163 subjects.

The primary outcome measure is handgrip strength. Secondary outcomes include several physical performance tests (six-minute walk test, timed up and go test, and standing on one leg with eyes closed). The dropout rate was 16.0%. The means (95% confidence interval) of change in handgrip strength for placebo, low-dose, and high-dose groups are -0.98(-2.04,0.08), 0.50(-0.65,1.65) and 1.03(-0.37,2.44) kg (P = 0.06, P for trend = 0.02), respectively. No significant effects of the interventions were observed for physical performances.

These findings suggest that pRJ treatment might not improve, but rather attenuate the progression of decrease in muscle strength in elderly people. In addition, we have not found that pRJ intervention can achieve improvement or attenuating the decrease in physical performance.