Monday, September 01, 2014

Honey Effective in Healing Cesarean Section Incisions

Oman Med J, 2014 Jul;29(4):255-9
The effect of honey gel on abdominal wound healing in cesarean section: a triple blind randomized clinical trial.
OBJECTIVE:
To assess whether honey can accelerate the wound healing in women undergoing cesarean section.
METHODS:
This was a triple blinded randomized prospective clinical trial. Women with cesarean section were randomly designated as drug (37 cases) and placebo (38 cases) groups. The drug group received local honey gel 25% while the placebo group received similar free-honey gel on abdominal cesarean incision twice a day for 14 days. REEDA scale (Redness, Edema, Ecchymosis, Discharge and Approximation of wound edges) was used to assess wound healing.
RESULTS:
The mean REEDA was 2.27 ± 2.46 and 3.91 ± 2.74 (p=0.008) on the 7(th) day and 0.47 ± 0.84 and 1.59± 1.95 (p=0.002) on the 14(th) day for the drug and placebo groups, respectively. Redness, edema and hematoma in the drug group were significantly lower on the 7(th) and 14(th) days.
CONCLUSION:
Honey was effective in healing the cesarean section incision. Using topical honey is suggested as a natural product with rare side effects in order to reduce the complications of cesarean wounds.

Sunday, August 31, 2014

Bee Drone Milk Induces Progestational Effects

Investigation of gestagenic effect of raw drone milk in rats
[Article in Hungarian]
Acta Pharm Hung, 2014;84(2):77-81
Numerous honeybee products are used in traditional medicine. The best-known honeybee products are the honey, the propolis and the royal jelly. Drone milk is a relatively little-known honeybee product. Although, drone milk is traditionally used to treat infertility and to promote vitality in both men and women in certain countries, the literature furnishes no information concerning effects of the drone milk. The oestrogenic and androgenic effects of drone milk have recently been reported in rats and the effective compounds have also been identified. The aim of this study was to determine the putative gestagenic effect of raw drone milk in rats. Maintenance of pregnancy assays revealed that drone milk was able to increase the number of surviving fetuses. This results suggested some gestagenic effects. This effect was confirmed by RT-PCR and Western blot methods in which the mRNA and protein expressions of gestagen-dependent CRLR (Calcitonin Receptor-Like Receptor) peptide were determined. To determine the efficacy of gestagenic effect of drone milk, spironolactone (weak gestagen compound) was used. The combination of drone milk and spironolactone showed more potent gestagenic effect. These results lead us to suppose that raw drone milk shows weak gestagenic effect and this effect can be increased by another weak gestagen. Further studies are required to clarify the gestagenic mechanisms of action of drone milk.

Saturday, August 30, 2014

New Zealand Company to Boost Manuka Honey Production

Landcorp to boost Manuka honey trials
Radio New Zealand, 8/29/2014
Field trials aimed at increasing production of high value, medical grade manuka honey are getting a boost from the country's biggest farmer Landcorp.
The state owned farming enterprise is planting out more than 90 hectares of manuka on its farms in the Te Anau area, Wairarapa and Canterbury…

Friday, August 29, 2014

Propolis May Help Prevent Kidney Damage

Role of propolis (bee glue) in improving histopathological changes of the kidney of rat treated with aluminum chloride
Environ Toxicol. 2014 Sep;29(9):1000-10
Humans are frequently exposed to aluminum from various food additives, therapeutic treatments and the environment, and it can be potentially toxic. This study is aimed to elucidate the protective effects of propolis against aluminum chloride (AlCl3 )-induced histopathological and immunohistochemical changes in kidney tissues of rats. Sixty Wistar Albino male rats (average weight 250-300 g) were divided into three equal groups. The first served as a negative control. The second received AlCl3 (34 mg/kg bw, 1/ 25 LD 50). The third were administered AlCl3 (34 mg/kg bw, 1/ 25 LD 50) plus propolis (50 mg/kg bw). Doses were given once daily via a gavage for 8 weeks every day. The results showed that shrunken glomeruli, intraglomerular congestion, loss of apical microvilli, degeneration of mitochondria and widened rough endoplasmic reticulum were also observed in the Proximal Convoluted Tubules of these animals.
Treatment with propolis ameliorated the harmful effects of AlCl3 ; this was also proved histopathologically by the noticeable improvement in the renal tissues. There were also significant variations in the expressed of ki-67 and p53 proteins. It can be concluded that propolis may be promising as a natural therapeutic agent in AlCl3 -induced renal toxicity and oxidative stress in rat kidneys.

Thursday, August 28, 2014

High Fructose Agave Syrups Show Antibacterial Activity Due to Manuka Honey Component Methylglyoxal

Methylglyoxal is associated with bacteriostatic activity of high fructose agave syrups
Food Chem. 2014 Dec 15;165:444-50
Three α-ketoaldehydes, potentially present in high fructose agave syrups (HFASs) as intermediates of the Maillard reaction, were determined. A previously reported HPLC-FLD procedure based on pre-column derivatisation with 4-methoxy-o-phenylenediamine was adopted, yielding the method quantification limits 0.11mg/kg, 0.10mg/kg, 0.09mg/kg for glyoxal, methylglyoxal (MGo) and diacetyl, respectively. The obtained results revealed high concentrations of methylglyoxal in HFASs (average 102±91mg/kg, range 15.6-315mg/kg) as compared to commercial Mexican bee honeys or corn syrups.
Hydrogen peroxide was generated in all HFASs upon dilution, yet to less extent than in bee honeys. HFASs presented bacteriostatic activity against Bacillus subtilis and Escherichia coli; catalase addition had minimum effect on the assay results in syrups with elevated MGo. Principal component analysis revealed direct association between growth inhibition and MGo. It is concluded that elevated concentration of MGo in HFASs is at least in part responsible for their non-peroxide bacteriostatic activity.

Wednesday, August 27, 2014

Bee Products Popular as Dietary Supplements in Malaysia

Determinants of Dietary Supplements Use among Adolescents in Malaysia
Asia Pac J Public Health. 2014 Sep;26(5 Suppl):36S-43S

Dietary supplements use is relatively widespread in some countries but knowledge of supplements consumption in Malaysia is limited, more so among adolescents. This study aimed to investigate the determinants of dietary supplements use among Malaysian adolescents using multiple logistic regressions analysis. Data from the Malaysia School-based Nutrition Survey 2012 based on a 2-stage stratified sampling was used. The prevalence of vitamin/mineral supplements and food supplements intake among adolescents was 54.1% and 40.2%, respectively. Usage was significantly higher among younger adolescents and among boys. Dietary supplements were also taken mostly by those who thought they were underweight.
The most common vitamin/mineral supplements and food supplements consumed were vitamin C and bee products. The main reason for taking supplements was parents' instruction. These findings can be useful for developing health communications on supplement use targeted to adolescents and their parents.

Tuesday, August 26, 2014

Bees Have Value in Medicine: Honey Helps Wounds Heal

Bees have value in medicine because their honey helps wounds heal. Honey is the thick liquid food bees make for themselves from flower nectar. At a time when drought, disease, parasites, pesticides, and Africanized swarms are killing off honey bees in large numbers around the world, it is important to consider the effects of bees beyond pollination.
Honey has antibacterial qualities and has been used in the practice of healing and medicine since ancient times. The Egyptians, Sumerians, Chinese, Greeks, and Romans all valued honey, and honey is mentioned in both the Bible and the Koran. While the healing powers of honey faded in comparison to the new antibiotics introduced in the 20th century, today’s antibiotic-resistant superbugs make any substance with bacteria-fighting properties worth a second look.
Honey is used effectively for wound care in hospitals and other medical settings around the world today. Derma Sciences, a Toronto-based company, manufactures a wound-care line called Medihoney, which includes honey from the blossoms of the manuka plant, Leptospermum scoparium, as an active ingredient in state-of-the-art wound dressings…

Monday, August 25, 2014

Florida Company Launches Energy Drink Made with Honey, Bee Pollen, Royal Jelly

EnerBee Organic Energy made with honey
FoodBev, 7/10/2014
Florida-based Natural Motives' EnerBee is an organic, lightly carbonated energy drink that contains honey, bee pollen and royal jelly…

Sunday, August 24, 2014

Eisai Launches Chocola BB Royal Jelly Drink in China

FooBev, 7/22/2014
Japan-based Eisai has launched Chocola BB Royal Drink in China.
The brand's latest beverage is designed to 'support beauty and wellness' and contains vitamin B6, royal jelly, taurine, collagen and goji berry…

Saturday, August 23, 2014

Antioxidant, Flavonoid Compounds of Sonication Propolis Extract Significantly Greater Than When Using Maceration

Antioxidant and anti-cancer cell proliferation activity of propolis extracts from two extraction methods
Asian Pac J Cancer Prev. 2013;14(11):6991-5
Antioxidant activity, total phenolic, total flavonoid compounds and cytotoxicity to cancer cell lines of propolis extracts from two extraction methods were investigated in this study. Propolis was collected from Phayao province and extracted with 70% ethanol using maceration and sonication techniques. The antioxidant activity was evaluated by DPPH assay. Total phenolic and flavonoid compounds were also determined. Moreover, the cytotoxicity of propolis was evaluated using MTT assay.
The percentage propolis yield after extraction using maceration (18.1%) was higher than using sonication (15.7%). Nevertheless, antioxidant and flavonoid compounds of the sonication propolis extract were significant greater than using maceration. Propolis extract from sonication showed antioxidant activity by 3.30 ± 0.15 mg gallic acid equivalents/g extract. Total phenolic compound was 18.3 ± 3.30 mg gallic acid equivalents/g extract and flavonoid compound was 20.49 ± 0.62 mg quercetin/g extract. Additionally, propolis extracts from two extraction methods demonstrated the inhibitory effect on proliferation of A549 and HeLa cancer cell lines at 24, 48 and 72 hours in a dose-dependent manner. These results are of interest for the selection of the most appropriate method for preparation of propolis extracts as potential antioxidant and anticancer agents.

Friday, August 22, 2014

Nanoparticles and Bee Venom May Help Fight Cancer

Fighting Cancer with Scorpion and Honeybee Venom
Newsweek, 8/20/2014
…Pan’s group created spherical nanoparticles that contain TsAP-1, a toxic protein from the venom of the Brazilian yellow scorpion, which has a potentially fatal sting. His team also created a similar product with melittin, the main toxin in bee venom, that killed breast cancer cells but didn’t hurt body cells.
The particles work by binding to receptors that are only present in cancerous cells, and then exposing the tumor to the toxin…

Thursday, August 21, 2014

Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann

BMC Genomics, 2014 Aug 8;15(1):665
BACKGROUND:
Most of the proteins contained in royal jelly (RJ) are secreted from the hypopharyngeal glands (HG) of young bees. Although generic protein composition of RJ has been investigated, little is known about how age-dependent changes on HG secretion affect RJ composition and their biological consequences. In this study, we identified differentially expressed proteins (DEPs) during HG development by using the isobaric tag for relative and absolute quantification (iTRAQ) labeling technique. This proteomic method increases the potential for new protein discovery by improving the identification of low quantity proteins.
RESULTS:
A total of 1282 proteins were identified from five age groups of worker bees, 284 of which were differentially expressed. 43 (15.1%) of the DEPs were identified for the first time. Comparison of samples at day 6, 9, 12, and 16 of development relative to day 3 led to the unambiguous identification of 112, 117, 127, and 127 DEPs, respectively. The majority of these DEPs were up-regulated in the older worker groups, indicating a substantial change in the pattern of proteins expressed after 3 days. DEPs were identified among all the age groups, suggesting that changes in protein expression during HG ontogeny are concomitant with different states of worker development. A total of 649 proteins were mapped to canonical signaling pathways found in the Kyoto Encyclopedia of Genes and Genomes (KEGG), which were preferentially associated with metabolism and biosynthesis of secondary metabolites. More than 10 key high-abundance proteins were involved in signaling pathways related to ribosome function and protein processing in the endoplasmic reticulum. The results were validated by qPCR.
CONCLUSION:
Our approach demonstrates that HG experienced important changes in protein expression during its ontogenic development, which supports the secretion of proteins involved in diverse functions in adult worker beyond its traditional role in royal jelly production.

Wednesday, August 20, 2014

Brazilian Green Propolis Improves Immune Function in Aged Mice

J Clin Biochem Nutr. 2014 Jul;55(1):7-10
Aging weakened innate and adaptive immunity both quantitatively and qualitatively. Some components in propolis could stimulate immune function in young animals or cultured immune cells in vitro. Few studies had been carried out in the aged. The present study was to evaluate the effects of Brazilian green propolis supplementation on the immunological parameters in aged mice.
Eighty Kunming mice, aged 15-18 months, were randomly assigned to the control and three experimental groups supplemented with different doses (83.3, 157.4 and 352.9 mg/kg.bw respectively) of Brazilian green propolis. The experiment lasted for 4 weeks. Contents of total polyphenol, flavonoid, cinnamic acid and artepillin-C in Brazilian green propolis were analyzed. Splenic NK cytotoxic, T lymphocyte proliferation and antibody generation cells, as well as the phagocytosis of peritoneal macrophages, ear swelling, and serum contents of IgG, IgM, hemolysin and cytokines were measured.
After 4 weeks of treatment, the phagocytosis of peritoneal macrophages was enhanced in 157.4 mg/kg and 352.9 mg/kg groups. Ear swelling increased in all propolis treatmented groups. Antibodies specific to sheep erythrocytes were higher in the groups receiving 157.4 and 352.9 mg/kg.bw than that of control group. IgG level dramatically increased in the groups receiving 83.3 and 157.4 mg/kg.bw in comparison to the control group.
These results indicate that administration of Brazilian green propolis have a positive effect on innate and adaptive immunity in aged mice.

Tuesday, August 19, 2014

Component of Wild Bee Venom Exhibits Antimicrobial Activity

Interaction of a novel antimicrobial peptide isolated from the venom of solitary bee Colletes daviesanus with phospholipid vesicles and Escherichia coli cells
J Pept Sci. 2014 Aug 14
The peptide named codesane (COD), consisting of 18 amino acid residues and isolated from the venom of wild bee Colletes daviesanus (Hymenoptera : Colletidae), falls into the category of cationic α-helical amphipathic antimicrobial peptides. In our investigations, synthetic COD exhibited antimicrobial activity against Gram-positive and Gram-negative bacteria and Candida albicans but also noticeable hemolytic activity. COD and its analogs (collectively referred to as CODs) were studied for the mechanism of their action.
The interaction of CODs with liposomes led to significant leakage of calcein entrapped in bacterial membrane-mimicking large unilamellar vesicles made preferentially from anionic phospholipids while no calcein leakage was observed from zwitterionic liposomes mimicking membranes of erythrocytes. The preference of CODs for anionic phospholipids was also established by the blue shift in the tryptophan emission spectra maxima when the interactions of tryptophan-containing COD analogs with liposomes were examined.
Those results were in agreement with the antimicrobial and hemolytic activities of CODs. Moreover, we found that the studied peptides permeated both the outer and inner cytoplasmic membranes of Escherichia coli. This was determined by measuring changes in the fluorescence of probe N-phenyl-1-naphthylamine and detecting cytoplasmic β-galactosidase released during the interaction of peptides with E. coli cells. Transmission electron microscopy revealed that treatment of E. coli with one of the COD analogs caused leakage of bacterial content mainly from the septal areas of the cells

Monday, August 18, 2014

Propolis Market Research Report 2014

DUBLIN — Research and Markets has announced the addition of the "Propolis (CAS 9009-62-5) Market Research Report2014" report to their offering. 
Propolis (CAS 9009-62-5) Market Research Report 2014 presents comprehensive data on propolis markets globally and regionally (Europe, Asia, North America etc.)
The report includes propolis description, covers its application areas and related patterns. It overviews propolis market, names propolis producers and indicates its suppliers…