Sunday, June 16, 2019

Honey an Effective Alternative Treatment for Toddlers with Diarrhea

The Effect of Honey with ORS and a Honey Solution in ORS on Reducing the Frequency of Diarrhea and Length of Stay for Toddlers

Compr Child Adolesc Nurs. 2019;42(sup1):21-28

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This study aims to identify the effect of honey with an oral rehydration solution (ORS) and a honey solution in ORS on the frequency of diarrhea and length of hospital stay for toddlers with diarrhea.

A randomized controlled trial was conducted in Padang and involved 72 toddlers aged 1-5 who were affected by diarrhea; the participants were selected through a probability sampling technique. The intervention group was provided with 5 ml of honey with an ORS and plain ORS at every diarrhea episode while the control group was provided with 10 ml of honey added to an ORS at every diarrhea episode. The findings suggest that there is a significant difference in the frequency of diarrhea between the intervention and control groups prior to and following the provision of honey with an ORS and honey added to an ORS (p < 0.05). T

here was a significant difference in the length of stay between both groups following the intervention and control treatments (p < 0.05). These results show that honey may be provided as an alternative therapy for toddlers with diarrhea.

Saturday, June 15, 2019

Propolis May Help Prevent Rot of Agricultural Products

Nanostructured chitosan/propolis formulations: characterization and effect on the growth of Aspergillus flavus and production of aflatoxins

Heliyon. 2019 May 24;5(5):e01776

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A great diversity of agricultural products is susceptible to contamination caused by Aspergillus flavus. To reduce fungal contamination, the application of natural products has been proposed, including chitosan and propolis, due to its broad and recognized antimicrobial activity on several microorganisms.

Currently, the application of nanotechnology allows for a greater activity to be more reactive and efficient. The objectives of this research were to characterize by TEM and Z potential some of the studied nanoparticles and to determine the in vitro antifungal activity of the formulations and the production of aflatoxins of the treated fungus. For this, individual treatments and different nanoformulations were elaborated by varying the percentage of the components such as chitosan solution, chitosan nanoparticles, an extract of propolis, nanoparticles of propolis, glycerol and canola oil.

The final concentrations of the formulations were of 20%, 30% and 40% and the control consisted of Czapeck-dox agar medium. TEM micrographies showed a spherical morphology in a range of 2.3-3.0 nm with values of Z potential from 18.5 to 116.2 nm. Compared to the untreated fungus, the highest effect was seen in the parameter of spore germination, since inhibition was of c. a. 97% corresponding to the formulation containing chitosan + propolis nanoparticles + chitosan nanoparticles + propolis extract at the highest concentration of 40%. At this same concentration, the production of aflatoxins was 100% inhibited with the treatment with chitosan at 1%.

Since these results are under carefully controlled conditions, further research should be extended to different fruit and vegetables affected by this fungus.

Friday, June 14, 2019

Honey Helps Heal Diabetic Foot Wounds (Diabetes)

Impact of olive oil and honey on healing of diabetic foot: a randomized controlled trial

Clin Cosmet Investig Dermatol. 2019 May 9;12:347-354

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Background: This study aimed 1) to examine the impact of honey on diabetic foot; 2) to examine the effect of olive oil on diabetic foot; and 3) to compare the impact of honey and olive oil in the healing of diabetic foot.

Methods: In this randomized controlled trial, 45 patients took part. Patients were randomly assigned to three groups. In the honey group, the wound was dressed using gauzes with honey daily for 1 month. In the olive oil group, the wound was dressed using gauzes with olive oil (4 mL) daily for 1 month. Patients in the control group received usual dressing. Wounds were assessed before and after intervention using the Wagner scoring system and the checklist of diabetic foot healing (where a higher score indicates better wound healing).

Results: Demographic characteristics of patients in the three groups were similar. Mean scores of tissue around the wound, wound grade, wound drainage, and wound healing were similar before intervention in all three groups. After intervention, means score of tissue around the wound, wound grade, wound drainage, and wound healing were significantly higher in patients in the honey and olive oil groups compared to patients in the control group.

Conclusion: The results of this study reveal that honey is as effective as olive oil in the treatment of diabetic foot. Given the few studies on this topic, further investigation is needed.

Wednesday, June 12, 2019

Egyptian Propolis Has Neuroprotective Effects

Impacts of Egyptian propolis extract on rat cerebellum intoxicated by aluminum silicate: histopathological studies

Environ Sci Pollut Res Int. 2019 May 29

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Human is exposed to traces of aluminum silicate (AlS), i.e., cosmetics, pesticides. Accumulation of aluminum compounds including AlS is associated with neuropathological diseases, e.g., Alzheimer's disease. The aim of the current study is to investigate the neuroprotective effects of propolis extracts in AlS-induced cerebellum intoxication in rats.

Forty adult rats were randomly divided into four groups (n = 10). The first group served as a control; the second group treated with 200 ml propolis/kg bwt. every other day by stomach gavage tube, third group was intraperitoneally injected with AIS (20 mg/kg) twice a week for 8 weeks, and the fourth group received propolis extract and AIS. At the end of 8 weeks, the cerebellum was harvested for further ultrastructure, histological, and histochemical investigations.

Using electron microscopy, the ultrastructure of the cerebellar cortex of AlS intoxicated rats showed Purkinje cells with an irregular euchromatic nucleus and extremely increased invagination of the nuclear envelope. In addition, the cytoplasm revealed numerous damaged mitochondria (> 20%) as well as swollen lysosomes (> 40%) compared to controls. These AlS-related ultrastructure changes were to some extent normalized to < 10% and < 30% in case of mitochondria and lysosomes, respectively, if rats were co-treated with propolis extract.

Further, histopathological examination showed that AlS-exposed rats revealed abnormal Purkinje cells with irregular size and shrank shape, evidence of pre-necrotic stage in the form of nuclear pyknosis, and condensed and frequent darkly stained cells in cerebellar layers. However, propolis extract co-administration reversed from 35 to 25% (p < 0.05) these alterations. The carbohydrate and protein contents were reduced in case of AlS treatment and surprisingly propolis co-treatment was able to rescue these neurotoxic effects.

Propolis extract might have neuroprotective effects against AIS-induced toxicity. Further studies are required to identify the mechanism of the pharmacological action and optimal settings for medical testing of propolis extract.

Tuesday, June 11, 2019

Propolis May Help Treat Sensitive Teeth

Treatment of dentinal hypersensitivity using propolis varnish: A scanning electron microscope study

Indian J Dent Res. 2019 Mar-Apr;30(2):249-253

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Background:

Dentin hypersensitivity (DH) affects all age groups in a population and is perceived as pain to any stimuli. From time immemorial, researchers have sought herbal or natural solutions to treat hypersensitivity. Propolis is one such natural, nontoxic resinous substance produced by honey bees, which is useful in various applications in dentistry and effective in treating dentinal hypersensitivity.

Aim:

The aim of this in vitro study is to assess the effect of propolis varnish on occlusion of dentinal tubules thus aiding in the treatment of dentinal hypersensitivity. The objective is to evaluate the effectiveness of the proposed treatment using scanning electron microscope (SEM) imaging.

Materials and Methods:

Twenty naturally extracted teeth were collected and stored until in vitro assessment. Discs obtained from each tooth were divided into two groups of 20 each - I (test) and II (control), with each tooth acting as its own control. Propolis varnish was applied only to the acid-etched surface of the exposed dentin of discs in the test group, whereas all the discs were subjected to SEM imaging.

Results:

Statistical analysis showed a significant reduction in open tubules (P < 0.001) from 160 ± 6.97 before treatment to 61.20 ± 9.10 after propolis varnish application in the test group.

Conclusion:

This study showed the promise of propolis varnish as a natural treatment modality for DH.

Monday, June 10, 2019

Propolis May Help Treat High Cholesterol and Obesity, Protect Heart (Hyperlipidemia, Dyslipidemia, Glucose Intolerance, Hypertension, Blood Pressure, Cardiovascular, Antioxidants)


Effect of Propolis on Diet-Induced Hyperlipidemia and Atherogenic Indices in Mice


Antioxidants (Basel). 2019 Jun 3;8(6). pii: E156

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Obesity, a major health problem worldwide, is associated with increased cardiovascular risk factors, such as dyslipidemia, glucose intolerance, and hypertension.

We investigated the antioxidative capacity of the ethanol extract of propolis (EEP) and its effect on the lipid profile, the hepatorenal function, and the atherogenic indices in mice fed with a high-fat diet (HFD). EEP (50 mg/kg) was given orally to mice for 30 days. After the treatments, levels of the serum total triglyceride and cholesterol, the high density lipoprotein (HDL-c) and low density lipoprotein (LDL-c) cholesterols, the serum enzymes, and the metabolites were measured, and atherogenic indices [atherogenic index of plasma (AIP); cardiac risk ratio (CRR); cardioprotective index (CPI); atherogenic coefficient (AC)] were calculated and compared with the antioxidant, the reducing power, the radical-scavenging, and the chelating activity of EEP.

The HFD diet with EEP significantly reduced the negative lipid profile and lowered AIP, CRR, and AC and increased CPI in animals on a HFD. In addition, EEP reduced the weight of mice and lipid accumulation in the liver, and it had significant in vitro antioxidative activities. The EEP possesses anti-hyperlipidemic and antioxidant activity and exhibits protective action on the cardiovascular system and hepatorenal functions.

Our results contribute towards the validation of the traditional use of propolis as a food supplement in aiding hyperlipidemic disorders.

Sunday, June 09, 2019

Spurge Honey and Garlic Combo Boosts Burn Wound Healing

Euphorbia honey and garlic: Biological activity and burn wound recovery

Burns. 2019 Jun 2. pii: S0305-4179(19)30077-4

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Currently, chronic wounds and microbial resistance to antibiotics have led to search new healing agents. Combinations of natural products are widely practiced in traditional medicine and exhibited synergistic activity with increased efficacy in treating several pathologies.

This study assays the antioxidant, synergistic antimicrobial and burn wound healing activities of Euphorbia honey and Allium sativum (garlic). The minimal inhibitory concentration (MIC) of each natural product was determined against microorganisms commonly found in wound infections.

The synergistic antimicrobial effect was assessed by mixing different concentrations of honey and garlic extract below their relative MICs. Subsequently, the antioxidant activity, total phenolic (TPC) and flavonoid (TFC) contents of both natural products and a selected mixture of them were evaluated. Efficacy of that mixture was also evaluated as topical application on male and female Wistar rats skin burn wound, compared to Euphorbia honey and two conventional treatments.

Results showed that the mixture honey-A. sativum has synergistic antimicrobial effect against all tested strains. Besides, A. sativum presented higher antioxidant activity along with higher TPC and TFC compared to honey and their mixture. However, the mixture showed higher wound healing activity reflected by shorter epithelialization and wound contraction time, as well as, better histological recovery of the treated tissues.

Our results also showed that burn wound healing is not affected by gender. Our findings support the idea of combining natural products as an effective therapy.

Friday, June 07, 2019

Propolis, Bee Pollen Have Beneficial Effect on Intestinal Morphology


Intestinal Morphology in Broiler Chickens Supplemented with Propolis and Bee Pollen

Animals (Basel). 2019 May 31;9(6). pii: E301

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The aim of this study was to determine the influence of dietary supplementation with propolis and bee pollen on the intestinal morphology and absorptive surface areas of chickens. Two hundred day-old Ross 308 chickens (100 male and 100 female) were equally allocated into five groups.

Throughout the whole study, the control group of chickens was fed with a basal diet, while the experimental groups of chickens were fed with the same diet supplemented with propolis and bee pollen: P1 = 0.25 g of propolis/kg + 20 g of bee pollen/kg; P2 = 0.5 g of propolis/kg; P3 = 1.0 g of propolis/kg; P4 = 20 g of bee pollen/kg.

The duodenal villi of chickens from all experimental groups were significantly higher and wider (p < 0.001), while their duodenal villi crypts were significantly deeper (p < 0.001) in comparison with these parameters in chickens from the control group. The villus height to crypt depth ratio, as well as the absorptive surface areas of broiler chickens, were significantly increased (p < 0.001) in experimental groups of chickens in comparison with the control group.

These findings suggest that dietary supplementation with propolis and bee pollen has a beneficial effect on broilers chickens' intestinal morphophysiology.

Thursday, June 06, 2019

Royal Jelly Component may Help Treat Inflammatory Disorders


Adenosine N1-Oxide Exerts Anti-inflammatory Effects through the PI3K/Akt/GSK-3β Signaling Pathway and Promotes Osteogenic and Adipocyte Differentiation

Biol Pharm Bull. 2019;42(6):968-976

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Previously, we reported that adenosine N1-oxide (ANO), which is found in royal jelly, inhibited the secretion of inflammatory mediators by activated macrophages and reduced lethality in lipopolysaccharide (LPS)-induced endotoxin shock. Here, we examined the regulatory mechanisms of ANO on the release of pro-inflammatory cytokines, with a focus on the signaling pathways activated by toll-like receptor (TLR)4 in response to LPS. ANO inhibited both tumor necrosis factor (TNF)-α and interleukin (IL)-6 secretion from LPS-stimulated RAW264.7 cells without affecting cell proliferation.

In this response, phosphorylation of mitogen-activated protein kinase (MAPK) family members (extracellular signal-regulated kinase (ERK)1/2, p38 and SAPK/c-Jun N-terminal kinase (JNK)) and nuclear factor-κB (NF-κB) p65 was not affected by treatment with ANO. In contrast, phosphorylation of Akt (Ser473) and its downstream molecule glycogen synthase kinase-3β (GSK-3β) (Ser9) was up-regulated by ANO, suggesting that ANO stimulated GSK-3β phosphorylation via phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. The phosphorylation of GSK-3β on Ser9 has been shown to negatively regulate the LPS-induced inflammatory response. Activation of PI3K/Akt signaling pathway has also been implicated in differentiation of mesenchymal stem cells into osteoblasts and adipocytes.

As expected, ANO induced alkaline phosphatase activity and promoted calcium deposition in a mouse pre-osteoblastic MC3T3-E1 cell line. The ANO-induced differentiation into osteoblasts was abrogated by coincubation with Wortmannin. Furthermore, ANO promoted insulin/dexamethasone-induced differentiation of mouse 3T3-L1 preadipocytes into adipocytes at much lower concentrations than adenosine. The protective roles of PI3K/Akt/GSK-3β signaling pathway in inflammatory disorders have been well documented.

Our data suggest that ANO may serve as a potential candidate for the treatment of inflammatory disorders. Promotion of osteogenic and adipocyte differentiation further suggests its application for regenerative medicine.

Wednesday, June 05, 2019

Honey Gel Boosts Healing of Burn Wounds


Wound-Healing Effect of Honey Gel and Film

J Pharm Bioallied Sci. 2019 Apr-Jun;11(2):176-180

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Background:

Honey has been formulated into gel and film dosage forms for burn wound as previously reported.

Aims:

In this study, we evaluated the ability of honey gel and film to promote the healing of burns and incision wounds on the skin of Sprague-Dawley female white rats.

Materials and Methods:

Twenty-four female rats were divided into four groups, which were treatment groups (for honey gel or film), negative control, and positive control (treated with marketed product "B"), respectively. Burn and incision wound were created by the method previously reported with slight modification. Parameters such as the percentage of wound closure and the tensile strength of the incision wound were determined.

Results:

The experimental results showed that honey film has a greater effectiveness to accelerate the healing for burns and incision wound in comparison to the negative control.

Conclusions:

Two-way analysis of variance indicates the type of treatment group, and time has a significant effect on the burn wound (P < 0.05). Honey film shows the significant difference (P < 0.05) with other group on the incision wound.

Monday, June 03, 2019

VIDEO: New Tool for Harvesting Bee Venom

ITN -WATCH THE VIDEO

An Australian researcher says he’s invented a new bee venom harvesting technique which doesn’t harm the insects.



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Sunday, June 02, 2019

Royal Jelly May Help Treat Male Infertility (sperm quality, hormonal and antioxidant status, reproductive organ histoarchitecture)

Attenuation potentials of royal jelly against hydroxyurea-induced infertility through inhibiting oxidation and release of pro-inflammatory cytokines in male rats

Environ Sci Pollut Res Int. 2019 May 24

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Hydroxyurea (HDU), a class of antineoplastic drugs, has a powerful efficacy in the treatment of several types of malignancies. However, it has multiple adverse effects including reduced fertility, especially in males.

Thus, 60 male albino rats were used to investigate the chemoprotective potentials of royal jelly on HDU-induced testicular damage. Animals were gastro-gavaged with HDU (225 or 450 mg kg-1 bw day-1) before royal jelly (100 mg kg-1 bw day-1) for 60 days. Blood samples and testicles were collected, and spermatozoon was obtained.

In a dose-dependent manner, the sperm count, motility and liveability, and testosterone, GSH, and catalase concentrations were decreased in HDU groups, whereas MDA, FSH, LH, IL-6, and IFN-γ expression levels were increased. Germinal epithelium degeneration, germ cell sloughing, reduction in the number of luminal spermatozoa, interstitial congestion, and severe leukocyte infiltration besides no glandular secretion in most of the acini were identified. However, royal jelly intake in HDU-treated rats successfully improved sperm quality, hormonal and antioxidant status, and reproductive organ histoarchitecture.

Thus, it could be concluded that royal jelly is endowed with antioxidative and anti-inflammatory activities and could be, therefore, used as an adjuvant remedy to improve HDU-induced male subfertility.

Saturday, June 01, 2019

Propolis Shows Neuroprotective Effects

Impacts of Egyptian propolis extract on rat cerebellum intoxicated by aluminum silicate: histopathological studies

Environ Sci Pollut Res Int. 2019 May 29

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Human is exposed to traces of aluminum silicate (AlS), i.e., cosmetics, pesticides. Accumulation of aluminum compounds including AlS is associated with neuropathological diseases, e.g., Alzheimer's disease.

The aim of the current study is to investigate the neuroprotective effects of propolis extracts in AlS-induced cerebellum intoxication in rats. Forty adult rats were randomly divided into four groups (n = 10). The first group served as a control; the second group treated with 200 ml propolis/kg bwt. every other day by stomach gavage tube, third group was intraperitoneally injected with AIS (20 mg/kg) twice a week for 8 weeks, and the fourth group received propolis extract and AIS.

At the end of 8 weeks, the cerebellum was harvested for further ultrastructure, histological, and histochemical investigations. Using electron microscopy, the ultrastructure of the cerebellar cortex of AlS intoxicated rats showed Purkinje cells with an irregular euchromatic nucleus and extremely increased invagination of the nuclear envelope. In addition, the cytoplasm revealed numerous damaged mitochondria (> 20%) as well as swollen lysosomes (> 40%) compared to controls. These AlS-related ultrastructure changes were to some extent normalized to < 10% and < 30% in case of mitochondria and lysosomes, respectively, if rats were co-treated with propolis extract.

Further, histopathological examination showed that AlS-exposed rats revealed abnormal Purkinje cells with irregular size and shrank shape, evidence of pre-necrotic stage in the form of nuclear pyknosis, and condensed and frequent darkly stained cells in cerebellar layers. However, propolis extract co-administration reversed from 35 to 25% (p < 0.05) these alterations. The carbohydrate and protein contents were reduced in case of AlS treatment and surprisingly propolis co-treatment was able to rescue these neurotoxic effects.

Propolis extract might have neuroprotective effects against AIS-induced toxicity. Further studies are required to identify the mechanism of the pharmacological action and optimal settings for medical testing of propolis extract.

Friday, May 31, 2019

Antibacterial Properties of Propolis

Molecules. 2019 May 29;24(11). pii: E2047

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Researchers are continuing to discover all the properties of propolis due to its complex composition and associated broad spectrum of activities.

This review aims to characterize the latest scientific reports in the field of antibacterial activity of this substance. The results of studies on the influence of propolis on more than 600 bacterial strains were analyzed.

The greater activity of propolis against Gram-positive bacteria than Gram-negative was confirmed. Moreover, the antimicrobial activity of propolis from different regions of the world was compared. As a result, high activity of propolis from the Middle East was found in relation to both, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) strains. Simultaneously, the lowest activity was demonstrated for propolis samples from Germany, Ireland and Korea.

Thursday, May 30, 2019

Honey, in Combination with Black Seed, Helps Control Asthma

Honey in Bronchial Asthma: From Folk Tales to Scientific Facts

J Med Food. 2019 May 24

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Bronchial asthma is one of the most common chronic inflammatory diseases. Complementary and alternative medicine is increasingly used for treating bronchial asthma.

Ten electronic databases were searched to investigate whether honey alone or in combination with other ingredients can be considered as the potential treatment for bronchial asthma.

Combinations of honey and Nigella sativa (NS) showed significant improvement in all pulmonary functions, including forced expiratory volume (FEV1) (MD = 0.52, P < .001), forced vital capacity (FVC) (MD = 0.55, P = .002), and peak expiratory flow rate (PEFR) (MD = 80.60, P < .001), in both moderate and severe, uncontrolled persistent asthma compared with baseline. 

Asthma control test scores also improved significantly (MD = 11.22, P < .001) in patients using combinations of honey and NS compared with baseline. Patients with a less severe grade of asthma showed a significant positive response in clinical parameters upon using honey.

One study showed that using celery seeds and honey was associated with clinical improvement of both lung functions, FEV1 (MD = 18.09, P < .001) and FVC (MD = 24.23, P < .001), and respiratory parameters compared with baseline.

In conclusion, honey alone has no strong evidence of being effective in controlling asthma. However, when used in combination with other substances, it showed a relatively high efficacy in patients with asthma. This finding may help in asthma control with lower cost alternatives and better outcomes.