Thursday, December 13, 2018

Propolis Helps Reduce Complications of Diabetes in Pregnancy

Propolis improves pregnancy outcomes and placental oxidative stress status in streptozotocin-induced diabetic rats

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BMC Complement Altern Med. 2018 Dec 5;18(1):324

BACKGROUND:

This study assessed the effects of propolis alone or combined with insulin on maternal status, pregnancy outcomes and placental oxidative stress in streptozotocin-induced diabetic rats.

METHODS:

Forty female rats were randomly assigned into five groups (n = 8/group) i.e. non-DM (non-diabetes), DM (diabetes), DM + Propolis (diabetes on propolis orally); DM + Insulin (diabetes on insulin subcutaneously) and DM + Combined (diabetes on propolis and insulin) groups. Propolis and insulin were given at 300 mg/kg/day orally and 5.0 IU/kg/day subcutaneously, respectively, for 4 weeks.

RESULTS:

Fasting blood glucose, conception period, implantation losses, foetal blood glucose and placental oxidative stress markers such as malonaldehyde and protein carbonyl were significantly higher while maternal weight gain, foetal body weight and total antioxidant capacity were significantly lower in DM group compared with non-DM group. These changes were significantly improved in rats treated with propolis or insulin alone with greater significant effects in rats treated with both propolis and insulin.

CONCLUSION:

This study may suggest the protective effects of propolis against DM-induced impaired pregnancy outcomes and placental oxidative stress with greater effects when combined with insulin.

...In conclusion, this study may suggest the protective effects of propolis against DM-induced impaired pregnancy outcomes and placental oxidative stress with greater effects when combined with insulin which could be partly due to the synergistic effect of some of its phytochemical constituents. Apart from supporting the traditional belief on the beneficial effect of propolis on health, further studies are suggested to determine its molecular mechanism of action and its possible role in protecting or reducing complications of DM in other organs.

Wednesday, December 12, 2018

Propolis Extract Cures Fungal Nail Infections (Onychomycosis)


Propolis for the Treatment of Onychomycosis

Indian J Dermatol. 2018 Nov-Dec;63(6):515-517

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Onychomycosis is a fungal nail infection, considered as a public health problem because it is contagious and it interferes with the quality of life. It has long and difficult treatment, with many side effects and high cost. Propolis extract (PE) is a potential alternative to conventional antifungal agents because it has low cost, accessibility, and low toxicity. Herein, we report the favorable response of PE in onychomycosis in three elderly patients...

In our in vitro experiments, PE was able to inhibit Candida spp. like other cases in the literature. In vivo, four nails were treated with PE of which two of them showed complete resolution. Culture was negative. The other two nails showed a reduction of over 50% of the lesion; however, fungi were still isolated. Based on the results obtained during in vitro studies as well as results obtained from tests performed after topical treatment, it can be concluded that propolis possesses antifungal potential and is a promising therapeutic option in cases of onychomycosis.

Tuesday, December 11, 2018

Manuka Honey May Help Treat Giardiasis and Trichomoniasis


Comparative effect of manuka honey on anaerobic parasitic protozoans with standard drug therapy under in vitro conditions: A preliminary study

Indian J Pharmacol. 2018 Jul-Aug;50(4):197-203

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BACKGROUND:

From the past five decades, metronidazole and tinidazole have been used for treating nonresistant and resistant giardiasis and trichomoniasis. However, due to the occurrence of drug resistance to standard therapy idealizes us to explore some additional therapies which is cost-effective, easy accessibility, and natural which has least side effects. Manuka honey obtained from Leptospermum scoparium is well known for its antibacterial and wound healing properties and is thought to be a better option as an additional therapy.

OBJECTIVE:

The present study was conducted to find out the effect of manuka honey on anaerobic protozoans that includes Giardia and Trichomonas under in vitro conditions in comparison to metronidazole and tinidazole.

MATERIALS AND METHODS:

Axenic culture of Giardia lamblia strain Portland 1 and Trichomonas vaginalis strain 413 was used for drug sensitivity assay to tinidazole, metronidazole, and manuka honey with the highest concentration of 17.1 μg/ml, 24.7 μg/ml, and 50%v/v by using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole). For this, head-to-head comparison has been done and IC 50 of the standard drug as well as manuka honey was calculated.

RESULTS:

The result showed that percentage inhibition on the growth of both the parasites is dependent on concentration as well as exposure time of the drug. The calculated IC 50 was found to be 5.6%v/v and 1.5%v/v for manuka honey with respect to G. lamblia and T. vaginalis.

CONCLUSION:

The present study suggests that manuka honey can be used as an additional therapy for the patient with giardiasis or trichomoniasis. However, in vivo study in the near future will elucidate more about the effectiveness of honey in treating parasitic infections.

Monday, December 10, 2018

Honey Effective as a Treatment in Otorhinolaryngology (rhinology, sinus, facial plastic and reconstructive surgery, laryngology, oral mucositis in cancer patients, cough, upper respiratory infection, pain control, tonsillectomy)

Honey as a Treatment in Otorhinolaryngology: A Review by Subspecialty.

Ann Otol Rhinol Laryngol. 2018 Dec 2:3489418815188

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OBJECTIVES:

To provide an up-to-date review of honey's effectiveness and potential applications in otorhinolaryngology.

METHODS:

A literature search of the online databases PubMed, EMBASE, and Cochrane Central Register of Controlled Trials was conducted.

RESULTS:

Sixty-three studies were identified within head and neck surgery (n = 23, 36%); pediatric otolaryngology (n = 18, 29%); rhinology, sinus, and skull base surgery (n = 11, 17%); otology (n = 6, 10%), facial plastic and reconstructive surgery (n = 3, 5%); and laryngology (n = 2, 3%). Studies included 6 meta-analyses, 44 randomized control trials, 5 case reports, and 8 animal models or in vitro studies. Of 55 clinical studies, 50 reported Level 1 evidence (prospective randomized control trials), and 5 reported Level 4 evidence (case series). The evidence level by subspecialty was: head and neck surgery (Level 1 n = 23), pediatrics (Level 1 n = 18), rhinology (Level 1 n = 7, level 4 n = 1), otology (Level 1 n = 1, Level 4 n = 3), facial plastics and reconstructive surgery (Level 4 n = 1), and laryngology (Level 1 n = 2).

CONCLUSIONS:

Honey can be used for a variety of otolaryngology conditions. The highest quality meta-analyses support oral honey for prevention and treatment of oral mucositis in cancer patients, cough associated with upper respiratory infection in children, and pain control after tonsillectomy. Further research will likely justify broader applications.

Sunday, December 09, 2018

Propolis Helps Get Rid of Papillomas and Warts at Home

Bee glue / propolis

You will need:

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2 drops of 50% tincture of propolis
Cotton
Bandage

What to do:

Apply the propolis to the affected area.

Cover the wart with a cotton pad.

Lock the disk with a bandage

Repeat this twice a day.

Why it works:

In the wild, bee propolis protects beehives against infections and has antiviral properties and action of antibiotic. Found that propolis activates volochkovu gland and stimulates the immune system.

Saturday, December 08, 2018

Propolis Credited with Alleviating Symptoms of Tonsillitis


Entrepreneur builds bee product line into budding powerhouse

By Hank Schultz

A personal health crisis while traveling led entrepreneur Carly Stein to bee products, and she subsequently founded a thriving supplement and foods brand and has been recognized by a major business publication.

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Friday, December 07, 2018

Palestinian Thyme, Jujube Honey Show Bioactive, Antioxidant Activities

Physicochemical characterization and antioxidant activity of Palestinian honey samples

Food Sci Nutr. 2018 Sep 11;6(8):2056-2065

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Physicochemical characteristics, main minerals, and antioxidant activity were determined for Palestinian honey samples belonging to different floral and geographical origins. The features of the analyzed samples were within the established international standards for honey quality control. One clear exception was the hydroxymethylfurfural (HMF) of the Ziziphus sample purchased from the Jericho region, which is the lowest city in the word characterized by a hot desert climate.

The observed HMF value was 81.86 ± 2.64 mg/kg being two folds the maximum allowed in honey samples (40 mg/kg). As a second objective of the present work, the parameters were divided into two groups with different discriminatory power. The assessed physicochemical parameters, and the antioxidant activities, specific to the botanical origin discrimination, were used to run the first PCA.

A strong correlation could be seen between the bioactive compounds and the antioxidant activities despite the geographical origin of the samples. Thyme and Ziziphus samples were the best samples, while citrus sample presented the lowest activity. Regarding the geographical discrimination, Ash and mineral contents in addition to the electrical conductivity were used. The output PCA conserved high represent ability of the data in the two-first components being 82.72% and 9.60%.

A little discrimination between the samples produced in the north and those produced in the south of the country, but it was not perfect. The intervention of the botanical variability could be the reason.

Thursday, December 06, 2018

Royal Jelly Could Aid Human Stem Cell Therapies


A Compound that Makes Bees Into Queens Could also Aid Human Stem Cell Therapies

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By Anna Groves | December 5, 2018

Scientists have announced a breakthrough in stem cell research that could have major implications for medical treatments that involve regenerating human cells. And their discovery came from an unlikely source: royal jelly. It’s the same substance honey bees use to turn a common bee larvae into a queen.

Royal Jelly

A queen bee starts her life just like any other bee – a larva in the honey bee hive, taken care of by her siblings. But when it’s time for a new queen, this larva is pulled aside and fed copious amounts of royal jelly, a nutritious goop secreted by the hive’s nurse bees. This, rather than any genetic differences, is what makes her a queen.

This queen bee grows larger than the other bees in the hive, which means she ends up with substantially more cells in her body, despite being genetically similar to her sisters. Royal jelly is thought to increase her cell count by somehow triggering an increase in stem cells — cells that haven’t yet differentiated into specialized cells — early in her development.

Royal jelly is popular as a dietary supplement in many areas of the world. Though many of the claimed effects on human health are of dubious merit, a few have been supported by randomized controlled trials. The latter inspired researchers to examine what effects the jelly might have outside of a bee hive.

Now, a research team out of Stanford University has found that the active compound in royal jelly, royalactin, prevents stem cells from differentiating, or growing into mature versions of, say, muscle or skin cells. This means that as they continue to grow and multiply they create more stem cells, increasing their therapeutic potential.

Wednesday, December 05, 2018

Royal Jelly Research Could Propel Cure for Alzheimer’s


Researchers say similar protein to royalactin in humans builds up ‘self-renewal’ stem cells

The Guardian

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It is the mysterious substance that turns worker honeybees into queens and fills the shelves of health food shops which tout its unverified powers to fend off ageing, improve fertility and reinvigorate the immune system.

Whether royal jelly has genuine health benefits for humans is a matter for more research, but in a study scientists have cracked one of the most enduring puzzles surrounding the milky gloop: the secret behind its queen-maker magic.

The discovery promises to have an impact far beyond the niche field of melittology. Armed with the findings, scientists are now exploring potential new treatments for wounds and disorders such as muscle wastage and neurodegenerative disease.

Researchers at Stanford University found that the main active component in royal jelly, a protein called royalactin, activates a network of genes that bolsters the ability of stem cells to renew themselves. It means that, with royalactin, an organism can produce more stem cells to build and repair itself with.

“We have a very identifiable avenue through which royal jelly’s effects are carried out,” said Kevin Wang, who led the Stanford team. “It has this activity of keeping stem cells in a self-renewing state.”

Tuesday, December 04, 2018

Bangladeshi Propolis Shows Antioxidant, Anti-Pain Activity

Antioxidant, brine shrimp lethality and analgesic properties of propolis from Bangladesh

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Food Biochemistry, Volume 42, Issue 5

October 2018

Propolis which is a natural resinous product from the honeybees, is becoming increasingly popular for its diverse therapeutic and industrial applications.

In this study, we report for the first time the antioxidant, cytotoxic, and antinociceptive activities of propolis from Bangladesh (BDP). The antioxidant activities were measured by biochemical analyses while cytotoxicity was evaluated by brine shrimp lethality bioassay. Analgesic activity was evaluated by tail immersion and acetic acid‐induced writhing models in mice. BDP contained higher concentrations of polyphenols, flavonoids, tannins, ascorbic acid, vitamin E, and reducing sugars, as compared to previous reports of propolis from several countries. BDP also exhibited higher free radical‐scavenging activities and a dose‐dependent reducing power activity indicating its superior antioxidant potential. Moreover, BDP extract was most toxic to brine shrimp nauplii, with 50% lethal concentration (LC50) of 57.99 µg/mL and exhibited considerable antinociceptive activities which may be mediated via central and peripheral inhibitory mechanisms.

Practical applications

BDP is a honeybee product, becoming increasingly popular due its potential contributions to human health. BDP is a rich source of natural antioxidants which has the potential to be used to ameliorate oxidative stress disorders and has useful antinociceptive activities. It may be useful as potential therapeutic agents against cancer, pesticidal agents, and for other cytotoxic uses.

Monday, December 03, 2018

Royal Jelly May Boost Sperm Count (Infertility)

Royal jelly protection on flunixin meglumine-induced spermiotoxicity and testicular degeneration in mice

Pol J Vet Sci. 2018 Sep;21(3):497-506

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Current study was designed to investigate the protective effects of royal jelly on Flunixin me- glumine (FM)-induced spermiotoxicity related to sperm concentration, abnormal spermatozoa count and histopathological changes in mice testis. The subjects were divided into five groups according to FM and/or royal jelly intake: Control group; group 1, FM alone (25 mg/kg, im); group 2, combination of FM (25 mg/kg, im) and royal jelly (200 mg/kg, oral); group 3, FM alone (50 mg/kg, im); and group 4, combination of FM (50 mg/kg, im) and royal jelly (200 mg/kg, oral). The animals were fed once daily for 15 days and they were sacrificed last day. Epididymal sperm concentration and abnormal spermatozoa count were noted. Testicular histological findings were evaluated. On purpose, organization of each animal was graded according to Johnsen's scoring to assess the spermatogenesis relying on seminiferous tubule cross-section scores. Comparing to controls, FM administration caused a decrease in sperm concentration (p⟨0.05), an increase in total abnormal spermatozoa rates (p⟨0.05) and more degenerative changes in testes in mice. Royal jelly supplementation ameliorated both sperm concentration and abnormal spermato- zoa (p⟨0.05) comparing to the control group. In conclusion, we suggested that royal jelly might have protective effects in the FM-induced reductions in epididymal sperm concentration and in- crease in abnormal spermatozoa rate.

Sunday, December 02, 2018

Propolis Component Sensitizes Chemoresistant Gastric Cancer Cells to Anti-Cancer Drugs

Caffeic acid phenethyl ester potentiates gastric cancer cell sensitivity to doxorubicin and cisplatin by decreasing proteasome function

Anticancer Drugs. 2018 Nov 26

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Caffeic acid phenethyl ester (CAPE) is a major propolis component that possesses a variety of pharmacological properties such as antioxidant and anticancer effects.

Herein, we investigated the effectiveness of CAPE on cytotoxicity of clinically used anticancer drugs, doxorubicin (DXR) and cisplatin (CDDP), in parental and the drug-resistant cells of stomach (MKN45) and colon (LoVo) cancers. Concomitant treatment with CAPE potentiated apoptotic effects of DXR and CDDP against the parental cells. The treatment significantly reduced the production of reactive oxygen species elicited by DXR but did not affect the DXR-mediated accumulation of 4-hydroxy-2-nonenal, a lipid peroxidation-derived aldehyde.

Intriguingly, treatment of parental MKN45 cells with CAPE alone reduced 26S proteasome-based proteolytic activities, in which a chymotrypsin-like activity was most affected. This effect of CAPE was the most prominent among those of eight flavonoids and nine cinnamic acid derivatives and was also observed in parental LoVo cells. In the DXR-resistant or CDDP-resistant cells, the chymotrypsin-like activity was highly up-regulated and significantly decreased by CAPE treatment, which sensitized the resistant cells to DXR and CDDP. Reverse transcription-PCR analysis showed that CAPE treatment led to downregulation of five proteasome subunits (PSMB1-PSMB5) and three immunoproteasome subunits (PSMB8-PSMB10) in DXR-resistant MKN45 cells.

The results suggest that CAPE enhances sensitivity of these cancer cells and their chemoresistant cells to DXR and CDDP, most notably through decreasing proteasome function. Thus, CAPE may be valuable as an adjuvant for DXR or CDDP chemotherapy in gastric cancer.

Saturday, December 01, 2018

Bee Venom Acupuncture May Help Boost Traditional Parkinson's Disease Treatment

EXPLORE

Available online 2 November 2018
In Press, Corrected Proof

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Bee venom acupuncture in addition to anti-Parkinsonian medications may improve activities of daily living and motor symptoms more than medication alone in idiopathic Parkinson's disease.


Friday, November 30, 2018

Columbian Propolis May Help Treat Osteosarcoma (Bone Cancer)

The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples

Phytother Res. 2018 Nov 28

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Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells.

The chemical composition was analyzed by GC-MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC50 ). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH-DA. Transwell assay was used to evaluate the invasiveness of propolis-treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids.

Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.

Thursday, November 29, 2018

Bee Venom Component May Help Slow Progression of Alzheimer's Disease


Bee venom phospholipase A2 ameliorates Alzheimer's disease pathology in Aβ vaccination treatment without inducing neuro-inflammation in a 3xTg-AD mouse model

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Sci Rep. 2018 Nov 26;8(1):17369

Alzheimer's disease (AD) is the most common form of dementia and is characterized by an imbalance between the production and clearance of amyloid-beta (Aβ) and tau proteins.

Although vaccination against Aβ peptide results in a dramatic reduction in Aβ pathology in experimental mouse models, the initial clinical trial for an active Aβ vaccine was halted early due to the development of acute meningoencephalitis in 6% of the immunized patients, which likely involved a T-cell mediated pro-inflammatory response.

In this study, we aimed to determine whether bee venom phospholipase A2 (bvPLA2) treatment would induce Tregs and ameliorate AD pathology without unwanted T cell-mediated inflammation.

First, we investigated the effects of bvPLA2 on the inflammatory infiltration caused by Aβ vaccination. Inflammatory aggregates of CD3+ T lymphocytes and macrophages were found in the brains and spinal cords of mice treated with Aβ. However, administration of bvPLA2 dramatically eliminated central nervous system inflammation following Aβ immunization.

In AD model mice (3xTg-AD mice), bvPLA2 administration significantly ameliorated cognitive deficits and reduced Aβ burdens in the brains of Aβ-vaccinated 3xTg-AD mice. Additionally, we examined brain glucose metabolism using positron emission tomography with 18F-2 fluoro-2-deoxy-D-glucose. Cerebral glucose uptake was considerably higher in the brains of Aβ-vaccinated 3xTg-AD mice that received bvPLA2 than those that did not.

The present study suggests that the modulation of Treg populations via bvPLA2 treatment may be a new therapeutic approach to attenuate the progression of AD in conjunction with Aβ vaccination therapy without an adverse inflammatory response.