Beeswax Component May Help Treat Ulcerative Colitis

[Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol].

Rev Peru Med Exp Salud Publica. 2017 Apr-Jun;34(2):176-182

OBJECTIVES.:

To investigate the effects of D-002, a mixture of 6 high molecular weight primary aliphatic alcohols, obtained from beeswax (Apis mellifera), on severe inflammatory ulcerative colitis (UC) induced by Dextran sulfate (DSS) and ethanol in rats (Ratus ratus).

MATERIALS AND METHODS.:

Rats were randomly distributed in six groups: a zero control to which no damage was caused, and five to which the UC was induced: a negative control (vehicle), three treated with D-002 (25, 100 and 400 mg/kg) and a positive control with sulfasalazine (200 mg/kg) (reference substance). Clinical manifestations (body weight variation, diarrhea and rectal bleeding), macroscopic and histological damage score, and myeloperoxidase (MPO) activity were quantified.

RESULTS.:

The oral treatment with D-002 (25, 100 and 400 mg/ kg) significantly prevented the decrease in body weight. The dose of 400 mg/kg reduced the presence of diarrhea and rectal bleeding, although its comparison with the negative control only reached statistical significance on diarrhea. D-002 (25, 100 and 400 mg/kg) significantly reduced the score of macroscopic lesions (40.0; 43.3 and 47.2% inhibition, respectively), the histological damage score (31.5; 53.7 and 67.1% inhibition, respectively) and the activity of MPO (73.2; 83.6 and 85.0% inhibition, respectively), compared to the negative control group. Sulfasalazine significantly reduced all variables studied.

CONCLUSIONS.:

D-002 (25, 100 and 400 mg/kg) significantly protected the colonic mucosa in rats with severe inflammatory UC induced by DSS and ethanol.

Webinar November 29 on Apitherapy and Veterinary Medicine

CAA Webinar with French Veterinarian/Apitherapist Philippe Garcia

Date: November 29, 2017 Time: 2:30 pm (EST) Host: Canadian Apitherapy Association

GO TO: https://www.canadian-apitherapy-association.ca/webinars/caa-webinar-with-french-veterinarianapitherapist-philippe-garcia/ 

Our first webinar talking about the use of apitherapy in the realm of Veterinary Medicine.

Welcome and enjoy the conference of our French Veterinarian/Apitherapist Philippe Garcia.

The Webinar will be directly translated in English by Yann Loranger, Secretary/Treasurer of the Canadian Apitherapy Association.

German Apitherapy Congress, Expo and Workshops March 23-27, 2018

The XVI-th. German Apitherapy Congress, Expo and Workshops with international participation, Passau, IBB Hotel City Centre, March 23-27, 2018. For more details contact Dr Stefan Stangaciu to drstangaciu@gmail.com

VIDEO: Apiterapia en Jaén "La Colmena Sanadora"

Anunciamos que por fin,tendremos Apiterapia en Jaén!

Bee Venom Peptide May Help Treat Liver Cancer

The anti-hepatocellular carcinoma activity of Mel-P15 is mediated by natural killer cells

Oncol Lett. 2017 Dec;14(6):6901-6906

Mel-P15 is a peptide derived from melittin, the main toxic component in the venom of the European honeybee Apis mellifera.

In the present study, the antitumor effects of Mel-P15 and the underlying molecular mechanisms of these effects in vivo were investigated. Mel-P15 directly stimulated natural killer (NK) cell cytotoxicity in vitro, which was increased to 55.45% at a 4 µg/ml dose of Mel-P15.

In the mouse liver cancer (H22) xenograft mice model, Mel-P15 suppressed tumor growth in vivo; the tumor inhibitory rate was 61.15% following treatment with 2 mg/kg Mel-P15. In addition, the immune response was activated following Mel-P15 treatment. Mel-P15 treatment increased the spleen and thymus indices, promoted splenocyte proliferation, stimulated NK cytotoxicity and upregulated the secretion of cytokines, including interleukin-2, interferon-γ and tumor necrosis factor-α.

In addition, the tumor inhibitory effect of Mel-P15 on BEL-7402-bearing nude mice was abrogated by the selective depletion of NK cells via the intraperitoneal injection of an anti-asialo GM-1 antibody.

The results suggest that Mel-P15 inhibits tumor growth in vivo by promoting NK cell cytotoxicity. Mel-P15 may therefore be a potential immunotherapy candidate for the treatment of hepatocellular carcinoma.

Propolis, Honey Component Shows Anti-Inflammatory, Anti0cancer, and Anti-Viral Activities

Chrysin: Sources, beneficial pharmacological activities, and molecular mechanism of action

Phytochemistry. 2017 Nov 18;145:187-196

In recent years, public and scientific interest in plant flavonoids has tremendously increased because of their postulated health benefits.

This review was mainly focuses on the flavone chrysin (5,7-dihydroxyflavone), which occurs naturally in many plants, honey, and propolis. A number of in vitro and in vivo studies have revealed the therapeutic effects of chrysin against various diseases.

In general, chrysin exhibits many biological activities and pharmacological effects, including antioxidant, anti-inflammatory, anticancer, and antiviral activities. Moreover, many studies have reported on the bioavailability of chrysin. Because of its compromised bioavailability and enhanced protein stability, chrysin solid lipid nanoparticle (SLN) synthesis avoids proteolytic degradation and sustained release of drug delivery.

To clarify the mechanism of action of chrysin, researchers have investigated the structural binding relationship of chrysin through the docking computation method.

Ukrainian Honey-Propolis Gum Prevents Periodontal Disease

Made in Ukraine: periodontal disease treatment chewing gum 

Ukraine Good News, 11/21/2017

Marta Tsap, an Ivano-Frankivsk National Medical University student, presented a chewing gum based on bactericidal and antiseptic properties of honey to prevent oral cavity diseases. The product is a great way to treat and prevent periodontal disease.

The chewing gum is based on caramelized honey, its effect was tested by the university dentistry faculty experts. The periodontium state of 6 out of 9 volunteers using the chewing gum for a month has improved.

In addition to honey, the gum contains wax, propolis and other beekeeping products.

As Marta says, the chewing gum is ready for serial production launch. The honey gum production start requires 209,000 UAH. The project will pay off in 2 years.

Propolis May Help Protect Against Radiation Damage

Radio-Modulatory Potential ofCaffeic Acid Phenethyl Ester: A Therapeutic Perspective

Anticancer Agents Med Chem. 2017 Nov 13

Use of natural agents is an upcoming area of research in cancer biology. Caffeic acid phenethyl ester has received great attention because of its therapeutic potential in various conditions including cancer. It is an active/abundant component of propolis. Propolis is a honey bee hive product produced by bees using their enzyme-rich digestive secretions on resinous mix, bee wax and pollen from plants. It is used to protect the beehive against bacteria and other infections.Although a lot of work has been done on chemotherapeutic aspects of CAPE, its role as a radiomodulator is yet to be delineated. It can act both as radioprotector and radiosensitizer. Depending on the tissue type it can modulate the radiation response by following different mechanisms.

This review will focus on the differential radiomodulatory effects of Caffeic Acid Phenethyl Ester in normal and cancer cells.Besides chemistry and bioavailability,it's potential as a therapeutic agent against radiation induced damage will also be discussed.

Propolis: This Bee Product is a Powerful Natural Immune Booster

By: Jordyn Cormier, November 19, 2017

Cold and flu season is in full swing, which means it’s time to start bringing on immune boosters again. Some of us will chug freshly squeezed orange juice. Others will slam shots of elderberry kombucha. A few will start force-feeding themselves and their family pungent raw garlic, all in a martyrish effort to avoid getting sick. But there is probably something even more powerful missing from your natural immune defense arsenal…propolis.

Propolis is one of the most undervalued natural immune boosters out there. Personally, I use my propolis spray daily. After years of getting sick every cold/flu season, a friend sent me some propolis to try. Ever since then, even the most inevitable colds seem to repel off of me. It’s pretty amazing.

If you are unfamiliar with propolis, it is a bee product, much like honey is—but that’s where the similarities end. Bees use propolis to keep their hives free of invaders and disease. It lines the hive walls and acts essentially as the immune system of the hive. And guess what—it can do the same for your body...

Turkish Honeys Beat Manuka Antibacterial Properties

Turkish honey beats world famous Manuka honey, study finds

DAILY SABAH

The flower honey from Turkey's southeastern province of Hakkari has much higher antimicrobial capabilities compared to the world famous Manuka honey of Australia and New Zealand, a new study has found.

Sevgi Kolaylı, a chemistry professor at the Karadeniz Technical University in Trabzon, says her recent study has found that several kinds of honey gathered from different parts of Turkey showed much higher antimicrobial activity compared to Manuka honey.

Kolaylı's study found that antimicrobial activities, which makes honey act against bacteria like antibiotics, are significantly higher in flower honey from Hakkari province compared to Manuka.

Several kinds of Turkish chestnut and oak honey also showed higher antibacterial properties, Kolaylı said.

The professor also said that Turkey ranks only behind China and Argentina as the world's top honey producing country.

"Antioxidant properties are richer in dark colored honey, whereas antimicrobial activities are higher in lighter colored honey," the professor explained, and added that Turkey is home to rich honey from both spectrums.

Ethanolic Extract of Polish Propolis Boosts Oral Hygiene

The Influence of Toothpaste Containing Australian Melaleuca alternifolia Oil and Ethanolic Extract of Polish Propolis on Oral Hygiene and Microbiome in Patients Requiring Conservative Procedures

Molecules. 2017 Nov 13;22(11). pii: E1957

The study was based on the use of a toothpaste with antiphlogistic activity, containing Australian Melaleuca alternifolia oil (tea tree oil-TTO) and ethanolic extract of Polish propolis (EEP). Fifty-one patients with varying conditions of the gingiva were divided into two groups.

The study group received the toothpaste with TTO and EEP, while the control group received the same toothpaste but without TTO and EEP. Approximal plaque index (API), simplified oral hygiene index (OHI-s) and modified sulcus bleeding index (mSBI) were assessed in three subsequent stages. During each examination, swabs were employed for microbiological inoculation. During the period of use of toothpastes with TTO and EEP, a significant reduction of the API was observed, as assessed upon the control visit after 7 days and after 28 days, compared to baseline. A statistically significant reduction of mSBI was observed after 7 and 28 days of using the toothpaste with TTO and EEP, as compared to the value upon the initial visit. Statistically significant differences in the OHI-s value were observed in the study group, which was using the active toothpaste. The use of a toothpaste containing TTO and EEP helps to maintain microbiome balance.

The observed stabilisation of bacterial microflora confirms the beneficial activity of toothpaste containing EEP and TTO compared to the control group, where the lack of these substances contributed to the emergence of qualitative and quantitative changes in oral microbiome.

Honey: A Therapeutic Agent for Disorders of the Skin

Cent Asian J Glob Health. 5(1):241

Problems with conventional treatments for a range of dermatological disorders have led scientists to search for new compounds of therapeutic value. Efforts have included the evaluation of natural products such as honey. Manuka honey, for example, has been scientifically recognised for its anti-microbial and wound healing properties and is now used clinically as a topical treatment for wound infections. In this review, scientific evidence for the effectiveness of honey in the treatment of wounds and other skin conditions is evaluated. A plethora of in vitro studies have revealed that honeys from all over the world have potent antimicrobial activity against skin relevant microbes. Moreover, a number of in vitro studies suggest that honey is able to modulate the skin immune system.

Clinical research has shown honey to be efficacious in promoting the healing of partial thickness burn wounds while its effectiveness in the treatment of non-burn acute wounds and chronic wounds is conflicted. Published research investigating the efficacy of honey in the treatment of other types of skin disorders is limited. Nevertheless, positive effects have been reported, for example, kanuka honey from New Zealand was shown to have therapeutic value in the treatment of rosacea. Anti-carcinogenic effects of honey have also been observed in vitro and in a murine model of melanoma.

It can be concluded that honey is a biologically active and clinically interesting substance but more research is necessary for a comprehensive understanding of its medicinal value in dermatology.

Powder Containing Extract of Green and Red Propolis a Possible Antimicrobial Agent

Evaluation of the antimicrobial activity and cytotoxic effect of hydroxyapatite containing Brazilian propolis

Biomed Mater. 2017 Nov 14

The aim of this work was to produce hydroxyapatite powder (HA) containing dry extract of green and red propolis, and to evaluate possible bactericidal activity of the materials in a short period of time through a fast release system.

The ethanolic extracts of green and red propolis (EEP) were incorporated into the material by spray drying. After release tests, powders containing dry EEP were characterized regarding the content of total phenolics and flavonoids. Materials characterization was made by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The antimicrobial activity was evaluated by plate colony counting, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against Staphylococcus aureus (S. aureus). The cytotoxicity of the materials was determined by the neutral red incorporation method. Materials showed apparently spherical morphology, indicating a decrease in the agglomeration degree with the propolis addition.

HA and propolis characteristic functional groups were observed in FTIR. Materials had higher release of phenolics and lesser amounts of flavonoids when compared to the EEP, with the higher amounts of flavonoids observed for HA with red propolis. The bactericidal effect for all materials was observed within the interval of 0.5 to 1 hour. All materials showed lesser inhibitory activity (MIC) and higher bactericidal activity (MBC) when compared to the EEP, with the best results attributed to HA with red propolis. The values of IC50 (concentration needed to inhibit 50% of the cell growth) obtained from the cytotoxicity assay for HA with green and red propolis lied between MIC and MCB.

Considering these results, it is suggested that HA with propolis may be used as a possible antimicrobial agent, inhibiting the growth of S. aureus, despite further in vivo biocompatibility should be investigated before using this material as a medical device with bactericidal potential.

Ginger and Propolis Exert Neuroprotective Effects

Ginger and Propolis Exert Neuroprotective Effects against Monosodium Glutamate-Induced Neurotoxicity in Rats

Molecules. 2017 Nov 8;22(11). pii: E1928

Central nervous system cytotoxicity is linked to neurodegenerative disorders. The objective of the study was to investigate whether monosodium glutamate (MSG) neurotoxicity can be reversed by natural products, such as ginger or propolis, in male rats.

Four different groups of Wistar rats were utilized in the study. Group A served as a normal control, whereas group B was orally administered with MSG (100 mg/kg body weight, via oral gavage). Two additional groups, C and D, were given MSG as group B along with oral dose (500 mg/kg body weight) of either ginger or propolis (600 mg/kg body weight) once a day for two months. At the end, the rats were sacrificed, and the brain tissue was excised and levels of neurotransmitters, ß-amyloid, and DNA oxidative marker 8-OHdG were estimated in the brain homogenates. Further, formalin-fixed and paraffin-embedded brain sections were used for histopathological evaluation.

The results showed that MSG increased lipid peroxidation, nitric oxide, neurotransmitters, and 8-OHdG as well as registered an accumulation of ß-amyloid peptides compared to normal control rats. Moreover, significant depletions of glutathione, superoxide dismutase, and catalase as well as histopathological alterations in the brain tissue of MSG-treated rats were noticed in comparison with the normal control. In contrast, treatment with ginger greatly attenuated the neurotoxic effects of MSG through suppression of 8-OHdG and β-amyloid accumulation as well as alteration of neurotransmitter levels.

Further improvements were also noticed based on histological alterations and reduction of neurodegeneration in the brain tissue. A modest inhibition of the neurodegenerative markers was observed by propolis.

The study clearly indicates a neuroprotective effect of ginger and propolis against MSG-induced neurodegenerative disorders and these beneficial effects could be attributed to the polyphenolic compounds present in these natural products.

Antimicrobial Effect of Ginger, Garlic, Honey, and Lemon Extracts on Streptococcus mutans

J Contemp Dent Pract. 2017 Nov 1;18(11):1004-1008.

The aim of this study was to evaluate the effect of lemon, ginger, garlic, and honey extracts on Streptococcus mutans.

MATERIALS AND METHODS:

Commercially obtained honey, ginger, garlic, and lemon were included in the study to evaluate its efficacy in isolation and in combination against S. mutans. The efficacies of extracts were tested using well diffusion method, and its effect was evaluated by measuring the zone of inhibition around the well. Antimicrobial activity of the extracts was carried out individually and compared considering triplicates of all the extracts.

RESULTS:

When individual comparison of the extracts was made, garlic showed greatest antimicrobial activity with a mean zone of inhibition (34.9 ± 0.58 mm) and honey showed least antimicrobial activity (0.5 ± 0.6 mm). When combinations of extracts were tested against S. mutans, lemon and garlic combination showed the greatest zone of inhibition (27.6 ± 0.43 mm) compared with other combinations, and ginger + lemon combinations showed the least zone of inhibition (12.6 ± 0.43 mm).

CONCLUSION:

This study concluded that garlic showed a greatest antimicrobial effect against S. mutans when compared with other preparations individually and garlic and lemon showed greatest zone of inhibition in combination than other preparations.

CLINICAL SIGNIFICANCE:

Antibiotics and other chemical agents are mainly used to treat the common dental infections. However, due to the excessive use, it can result in antibiotic resistance. Hence, herbal medicines with medicinal values should be replaced with conventional methods.