Saturday, June 04, 2011
Evidence-Based Complementary and Alternative Medicine, Volume 2011 (2011), Article ID 201953, 8 pages
Propolis, a resinous compound produced by Apis mellifera L. bees, is known to possess a variety of biological activities and is applied in the therapy of various infectious diseases.
The aim of this study was to evaluate the in vitro antifungal activity of propolis ethanol extract (PE) and propolis microparticles (PMs) obtained from a sample of Brazilian propolis against clinical yeast isolates of importance in the vulvovaginal candidiasis (VVC).
PE was used to prepare the microparticles. Yeast isolates (n=89), obtained from vaginal exudates of patients with VVC, were exposed to the PE and the PMs. Moreover, the main antifungal drugs used in the treatment of VVC (Fluconazole, Voriconazole, Itraconazole, Ketoconazole, Miconazole and Amphotericin B) were also tested. Minimum inhibitory concentration (MIC) was determined according to the standard broth microdilution method. Some Candida albicans isolates showed resistance or dose-dependent susceptibility for the azolic drugs and Amphotericin B. Non-C. albicans isolates showed more resistance and dose-dependent susceptibility for the azolic drugs than C. albicans. However, all of them were sensitive or dose-dependent susceptible for Amphotericin B.
All yeasts were inhibited by PE and PMs, with small variation, independent of the species of yeast.
The overall results provided important information for the potential application of PMs in the therapy of VVC and the possible prevention of the occurrence of new symptomatic episodes.
…In conclusion, considering the antifungal activity showed by PMs and that the high ethanol concentration is a disadvantage of PE, this report clearly showed that PMs arises as a possible agent for the treatment and especially the prevention of the new symptomatic episode the VVC. Moreover PMs have the advantage of to be incorporated in some dosage forms, like vaginal ointments, and to be administered into the vaginal mucosa more easily and safely.