Galangin Induces B16F10 Melanoma Cell Apoptosis Via
Mitochondrial Pathway and Sustained Activation of p38 MAPK
Cytotechnology, 2012 Sep 22
Galangin, an active flavonoid present at high concentration
in Alpinia officinarum Hance and propolis, shows cytotoxicity towards several
cancer cell lines, including melanoma. However, the specific cellular targets
of galangin-induced cytotoxicity in melanoma are still unknown.
Here, we investigated the effects of galangin in B16F10
melanoma cells and explored the possible molecular mechanisms. Galangin
significantly decreased cell viability of B16F10 cells, and also induced cell
apoptosis shown by Hoechst 33342 staining and Annexin V-PI double staining flow
cytometric assay.
Furthermore, upon galangin treatment, disruption of
mitochondrial membrane potential was observed by JC-1 staining. Western
blotting analysis indicated that galangin activated apoptosis signaling
cascades by cleavage of procaspase-9, procaspase-3 and PARP in B16F10 cells.
Moreover, galangin significantly induced activation of phosphor-p38 MAPK in a
time and dose dependent manner. SB203580, an inhibitor of p38, partially
attenuated galangin-induced apoptosis in B16F10 cells.
Taken together, this work suggests that galangin has the
potential to be a promising agent for melanoma treatment and may be further
evaluated as a chemotherapeutic agent.
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