Hydrogen peroxide-dependent antibacterial action of Melilotus albus honeyLett Appl Microbiol. 2017 Apr 20
Honey originating from different floral sources exhibits the broad-spectrum of antibacterial activity as a result of the presence of hydrogen peroxide as well as non peroxide bioactive compounds. The mechanisms of antibacterial activity of Polish melilot honey were investigated for the first time.
Polish melilot honey samples (Melilotus albus biennal -3 and annual-5, Melilotus officinalis -1) were collected directly from beekeepers and analyzed for pollen profile, basic physicochemical parameters, antioxidant capacity, radical scavenging activity, total phenolics contents as well as antibacterial properties against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella spp.
The physicochemical properties of melilot honey were specific for light-colored unifloral honeys and were not dependent on its botanical and geographical origin (P > 0.05). All tested honey samples exhibited inhibitory activity (above 90%) against Gram positive bacteria in the concentration of 12.5 to 25%. Above 30-50% of antibacterial activity of melilot honey was connected with glucose-oxidase enzyme action and was destroyed in the presence of catalase.
Hydrogen peroxide-dependent antibacterial activity of honey was inversely correlated with its radical scavenging activity (r = -0.67) and phenolic compounds ( r = -0.61). Antibacterial action of melilot honey depends not only on hydrogen peroxide produced by glucose-oxidase but also on other non peroxide bioactive components of honey.
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