Potential role of propolis in wound healing: Biological properties and therapeutic activities
Biomed Pharmacother. 2017 Dec 26;98:469-483
Propolis is a resinous mixture that honey bees collect from the tree buds, sap flows, or other botanical sources. The chemical composition of propolis varies and depends on the geographical area, time of collection, seasonality, illumination, altitude, and food availability during propolis exploitation. The goal of this review is to discuss important concepts including mechanisms of action and therapeutic activities of propolis.
The PubMed, ScienceDirect, and Cochrane Library databases were searched for the literature published from January the 1st 2000 to October the 1st 2017. Sixteen animals and three clinical studies were included. A quantitative and qualitative review was performed on the clinical trials and the animal studies were comprehensively overviewed. In this study, the clinical trials have been combined and the results were provided as meta-analysis. Propolis is a non-toxic natural product; however some cases of allergy and contact dermatitis to this compound have been described mainly among beekeepers. An important factor in impaired wound healing is biofilm formation; propolis as an anti-microbial agent can reduce biofilm generation and result in accelerated healing processes.
Most of the in vivo studies on various wound models suggested the beneficial roles of propolis on experimental wound healing and this has also been approved in the clinical trial studies. However, there is a lack of information concerning, dose, side effects and clinical effectiveness of propolis on wounds. As the effectiveness of propolis between different products is variable, more characterizations should be done and future investigations comparing different propolis based products and characterization of their specific roles on different models of wounds are highly appreciated.
Sunday, December 31, 2017
Saturday, December 30, 2017
Honeybee-Inspired Needles Decrease Insertion Force
Novel Design of Honeybee-inspired Needles for Percutaneous Procedure
Bioinspir Biomim. 2017 Dec 20
The focus of this paper is to present new designs of innovative bioinspired needles to be used during percutaneous procedures.
Insect stingers have been known to easily penetrate soft tissues. Bioinspired needles mimicking the barbs in a honeybee stinger were developed for a smaller insertion force, which can provide a less invasive procedure. Decreasing the insertion force will decrease the tissue deformation, which is essential for a more accurate targeting. In this study, some design parameters, in particular, barb shape and geometry (i.e., front angle, back angle, and height) were defined and their effects on the insertion force were investigated. Three-dimensional (3D) printing technology was used to manufacture bioinspired needles.
A specially-designed insertion test setup using tissue mimicking Polyvinyl chloride (PVC) gels was developed to measure the insertion and extraction forces. The barb design parameters were then experimentally modified through detailed experimental procedures to further reduce the insertion force. Different scales of the barbed needles were designed and used to explore the size-scale effect on the insertion force. To further investigate the efficacy of the proposed needle design in real surgeries, preliminary ex-vivo insertion tests into bovine liver tissue were performed.
Our results show that the insertion force of the needles in different scales decreased by 21-35% in PVC gel insertion tests and by 46% in bovine liver tissue insertion tests.
Bioinspir Biomim. 2017 Dec 20
The focus of this paper is to present new designs of innovative bioinspired needles to be used during percutaneous procedures.
Insect stingers have been known to easily penetrate soft tissues. Bioinspired needles mimicking the barbs in a honeybee stinger were developed for a smaller insertion force, which can provide a less invasive procedure. Decreasing the insertion force will decrease the tissue deformation, which is essential for a more accurate targeting. In this study, some design parameters, in particular, barb shape and geometry (i.e., front angle, back angle, and height) were defined and their effects on the insertion force were investigated. Three-dimensional (3D) printing technology was used to manufacture bioinspired needles.
A specially-designed insertion test setup using tissue mimicking Polyvinyl chloride (PVC) gels was developed to measure the insertion and extraction forces. The barb design parameters were then experimentally modified through detailed experimental procedures to further reduce the insertion force. Different scales of the barbed needles were designed and used to explore the size-scale effect on the insertion force. To further investigate the efficacy of the proposed needle design in real surgeries, preliminary ex-vivo insertion tests into bovine liver tissue were performed.
Our results show that the insertion force of the needles in different scales decreased by 21-35% in PVC gel insertion tests and by 46% in bovine liver tissue insertion tests.
Friday, December 29, 2017
By-Products From Beeswax Recycling Process Represent Rich Source f Phytochemicals with Antioxidant Capacity and Anticancer Activity
Are by-products from beeswax recycling process a new promising source of bioactive compounds with biomedical properties?
Food Chem Toxicol. 2017 Dec 25. pii: S0278-6915(17)30789-5
During the process of beeswax recycling, many industrial derivatives are obtained. These matrices may have an interesting healthy and commercial potential but to date they have not been properly studied.
The aim of the present work was to evaluate the proximal and phytochemical composition, the antioxidant capacity and cytotoxic effects of two by-products from beeswax recycling process named MUD 1 and MUD 2 on liver hepatocellular carcinoma. Our results showed that MUD 1 presented the highest (P < .05) fiber, protein, carbohydrate, polyphenol and flavonoid concentration, as well as the highest (P < .05) total antioxidant capacity than the MUD 2 samples. MUD1 exerted also anticancer activity on HepG2 cells, by reducing cellular viability, increasing intracellular ROS levels and affecting mitochondrial functionality in a dose-dependent manner.
We showed for the first time that by-products from beeswax recycling process can represent a rich source of phytochemicals with high total antioxidant capacity and anticancer activity; however, further researches are necessary to evaluate their potentiality for human health by in vivo studies.
Food Chem Toxicol. 2017 Dec 25. pii: S0278-6915(17)30789-5
During the process of beeswax recycling, many industrial derivatives are obtained. These matrices may have an interesting healthy and commercial potential but to date they have not been properly studied.
The aim of the present work was to evaluate the proximal and phytochemical composition, the antioxidant capacity and cytotoxic effects of two by-products from beeswax recycling process named MUD 1 and MUD 2 on liver hepatocellular carcinoma. Our results showed that MUD 1 presented the highest (P < .05) fiber, protein, carbohydrate, polyphenol and flavonoid concentration, as well as the highest (P < .05) total antioxidant capacity than the MUD 2 samples. MUD1 exerted also anticancer activity on HepG2 cells, by reducing cellular viability, increasing intracellular ROS levels and affecting mitochondrial functionality in a dose-dependent manner.
We showed for the first time that by-products from beeswax recycling process can represent a rich source of phytochemicals with high total antioxidant capacity and anticancer activity; however, further researches are necessary to evaluate their potentiality for human health by in vivo studies.
Thursday, December 28, 2017
Volatile Compounds in Brazilian, Argentinian and Venezuelan Propolis Analysed
Comparative Analysis of Volatile Compound Profiles of Propolis From Different Provenances
J Sci Food Agric. 2017 Dec 27
BACKGROUND:
Propolis is a complex mixture that honey bees produce from the exudates of various plants and presents many medicinal properties. Its chemical compositions varies according to the phytogeography characteristics of each region, among others. The aim of this study was to identify and characterize the volatile organic compounds (VOCs) present in Venezuelan propolis and compare with reference samples such as Brazilian and Argentinian ones.
RESULTS:
A total of 90 VOCs were identified in a series of propolis samples using both Solid-Phase Microextraction (SPME) and Dynamic Headspace (DHS), both coupled to C-EI-MS. In the case of Venezuelan propolis, sesquiterpenes, esters, aromatic compounds and aliphatic hydrocarbons were identified. The limonene was found only in Venezuelan samples being the first time it is identified in samples from this country. In the case of green propolis, β-caryophyllene and nerolidol were the major ones. As for the Argentinian samples, the prenyl acetate, benzyl acetate, and 2-phenylethyl acetate were detected only in these samples.
CONCLUSIONS:
Possible chemical markers of natural sources such as limonene were detected using DHS extraction. Several compounds have also been identified for the first time in Venezuelan propolis. Cluster analysis allowed to relate propolis VOCs profile with their provenance.
J Sci Food Agric. 2017 Dec 27
BACKGROUND:
Propolis is a complex mixture that honey bees produce from the exudates of various plants and presents many medicinal properties. Its chemical compositions varies according to the phytogeography characteristics of each region, among others. The aim of this study was to identify and characterize the volatile organic compounds (VOCs) present in Venezuelan propolis and compare with reference samples such as Brazilian and Argentinian ones.
RESULTS:
A total of 90 VOCs were identified in a series of propolis samples using both Solid-Phase Microextraction (SPME) and Dynamic Headspace (DHS), both coupled to C-EI-MS. In the case of Venezuelan propolis, sesquiterpenes, esters, aromatic compounds and aliphatic hydrocarbons were identified. The limonene was found only in Venezuelan samples being the first time it is identified in samples from this country. In the case of green propolis, β-caryophyllene and nerolidol were the major ones. As for the Argentinian samples, the prenyl acetate, benzyl acetate, and 2-phenylethyl acetate were detected only in these samples.
CONCLUSIONS:
Possible chemical markers of natural sources such as limonene were detected using DHS extraction. Several compounds have also been identified for the first time in Venezuelan propolis. Cluster analysis allowed to relate propolis VOCs profile with their provenance.
Propolis May Help Treat White-Nose Syndrome in Bats
Evidence for Anti-Pseudogymnoascus destructans (Pd) Activity of Propolis
Antibiotics (Basel). 2017 Dec 21;7(1)
White-nose syndrome (WNS) in bats, caused by Pseudogymnoascus destructans (Pd), is a cutaneous infection that has devastated North American bat populations since 2007. At present, there is no effective method for controlling this disease.
Here, we evaluated the effect of propolis against Pd in vitro. Using Sabouraud dextrose agar (SDA) medium, approximately 1.7 × 10⁷ conidia spores of the Pd strain M3906-2/mL were spread on each plate and grown to form a consistent lawn. A Kirby-Bauer disk diffusion assay was employed using different concentrations of propolis (1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%), in plates incubated at 8 °C and 15 °C. At 8 °C and 15 °C, as the concentration of propolis increased, there was an increasing zone of inhibition (ZOI), reaching the highest degree at 10% and 25% concentrations, respectively.
A germule suppression assay showed a similar effect on Pd conidia germination. A MALDI-TOF-MS analysis of propolis revealed multiple constituents with a potential anti-Pd activity, including cinnamic acid, p-coumaric acid, and dihydrochalcones, which could be further tested for their individual effects. Our study suggests that propolis or its individual constituents might be suitable products against Pd.
Antibiotics (Basel). 2017 Dec 21;7(1)
White-nose syndrome (WNS) in bats, caused by Pseudogymnoascus destructans (Pd), is a cutaneous infection that has devastated North American bat populations since 2007. At present, there is no effective method for controlling this disease.
Here, we evaluated the effect of propolis against Pd in vitro. Using Sabouraud dextrose agar (SDA) medium, approximately 1.7 × 10⁷ conidia spores of the Pd strain M3906-2/mL were spread on each plate and grown to form a consistent lawn. A Kirby-Bauer disk diffusion assay was employed using different concentrations of propolis (1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%), in plates incubated at 8 °C and 15 °C. At 8 °C and 15 °C, as the concentration of propolis increased, there was an increasing zone of inhibition (ZOI), reaching the highest degree at 10% and 25% concentrations, respectively.
A germule suppression assay showed a similar effect on Pd conidia germination. A MALDI-TOF-MS analysis of propolis revealed multiple constituents with a potential anti-Pd activity, including cinnamic acid, p-coumaric acid, and dihydrochalcones, which could be further tested for their individual effects. Our study suggests that propolis or its individual constituents might be suitable products against Pd.
Wednesday, December 27, 2017
Propolis Better Than Aciclovir for Treating Cold Sores
Comparative Study with a Lip Balm Containing 0.5% Propolis Special Extract GH 2002 versus 5% Aciclovir Cream in Patients with Herpes Labialis in the Papular/Erythematous Stage: A Single-blind, Randomized, Two-arm Study.
Curr Ther Res Clin Exp. 2017 Oct 14;88:1-7
BACKGROUND AND OBJECTIVES:
This controlled single-blind trial compared the efficacy of a lip balm with propolis special extract GH 2002 at a concentration of 0.5% in the treatment of episodes of herpes labialis with that of 5% aciclovir cream.
METHODS:
Patients in the erythematous/papular stage were randomized: 189 patients were treated with propolis cream, 190 patients were treated with aciclovir cream (intention-to-treat population). Application was 5 times daily. The primary parameter was the difference in median time to complete encrustation or epithelialization of lesions. Secondary parameters were the development of typical herpes symptoms (eg, pain, burning and itching, tension, and swelling), the global assessment of efficacy, and the safety of application.
RESULTS:
The predefined clinical situation was reached after a median of 4 days with propolis and after 5 days with aciclovir (P < 0.0001). Significant differences in favor of the study preparation were found with all secondary parameters and symptoms. No allergic reactions, local irritations, or other adverse events were observed.
CONCLUSIONS:
A formulation of 0.5% propolis GH 2002 extract lip balm was found to be superior in the treatment of episodes of herpes labialis over 5% aciclovir cream in patients in the papular/erythematous phase upon inclusion.
Curr Ther Res Clin Exp. 2017 Oct 14;88:1-7
BACKGROUND AND OBJECTIVES:
This controlled single-blind trial compared the efficacy of a lip balm with propolis special extract GH 2002 at a concentration of 0.5% in the treatment of episodes of herpes labialis with that of 5% aciclovir cream.
METHODS:
Patients in the erythematous/papular stage were randomized: 189 patients were treated with propolis cream, 190 patients were treated with aciclovir cream (intention-to-treat population). Application was 5 times daily. The primary parameter was the difference in median time to complete encrustation or epithelialization of lesions. Secondary parameters were the development of typical herpes symptoms (eg, pain, burning and itching, tension, and swelling), the global assessment of efficacy, and the safety of application.
RESULTS:
The predefined clinical situation was reached after a median of 4 days with propolis and after 5 days with aciclovir (P < 0.0001). Significant differences in favor of the study preparation were found with all secondary parameters and symptoms. No allergic reactions, local irritations, or other adverse events were observed.
CONCLUSIONS:
A formulation of 0.5% propolis GH 2002 extract lip balm was found to be superior in the treatment of episodes of herpes labialis over 5% aciclovir cream in patients in the papular/erythematous phase upon inclusion.
Tuesday, December 26, 2017
Portuguese Honeys Possess Excellent Anti-Biofilm Activity
Synergistic Antimicrobial Interaction between Honey and Phage against Escherichia coli Biofilms
Front Microbiol. 2017 Dec 8;8:2407
Chronic wounds afford a hostile environment of damaged tissues that allow bacterial proliferation and further wound colonization. Escherichia coli is among the most common colonizers of infected wounds and it is a prolific biofilm former.
Living in biofilm communities, cells are protected, become more difficult to control and eradicate, and less susceptible to antibiotic therapy. This work presents insights into the proceedings triggering E. coli biofilm control with phage, honey, and their combination, achieved through standard antimicrobial activity assays, zeta potential and flow cytometry studies and further visual insights sought by scanning electron microscopy and transmission electron microscopy.
Two Portuguese honeys (PF2 and U3) with different floral origin and an E. coli-specific phage (EC3a), possessing depolymerase activity, were tested against 24- and 48-h-old biofilms. Synergic and additive effects were perceived in some phage-honey experiments.
Combined therapy prompted similar phenomena in biofilm cells, visualized by electron microscopy, as the individual treatments. Honey caused minor membrane perturbations to complete collapse and consequent discharge of cytoplasmic content, and phage completely destroyed cells leaving only vesicle-like structures and debris.
Our experiments show that the addition of phage to low honey concentrations is advantageous, and that even fourfold diluted honey combined with phage, presents no loss of antibacterial activity toward E. coli. Portuguese honeys possess excellent antibiofilm activity and may be potential alternative therapeutic agents in biofilm-related wound infection. Furthermore, to our knowledge this is the first study that assessed the impacts of phage-honey combinations in bacterial cells.
The synergistic effect obtained was shown to be promising, since the antiviral effect of honey limits the emergence of phage resistant phenotypes.
Front Microbiol. 2017 Dec 8;8:2407
Chronic wounds afford a hostile environment of damaged tissues that allow bacterial proliferation and further wound colonization. Escherichia coli is among the most common colonizers of infected wounds and it is a prolific biofilm former.
Living in biofilm communities, cells are protected, become more difficult to control and eradicate, and less susceptible to antibiotic therapy. This work presents insights into the proceedings triggering E. coli biofilm control with phage, honey, and their combination, achieved through standard antimicrobial activity assays, zeta potential and flow cytometry studies and further visual insights sought by scanning electron microscopy and transmission electron microscopy.
Two Portuguese honeys (PF2 and U3) with different floral origin and an E. coli-specific phage (EC3a), possessing depolymerase activity, were tested against 24- and 48-h-old biofilms. Synergic and additive effects were perceived in some phage-honey experiments.
Combined therapy prompted similar phenomena in biofilm cells, visualized by electron microscopy, as the individual treatments. Honey caused minor membrane perturbations to complete collapse and consequent discharge of cytoplasmic content, and phage completely destroyed cells leaving only vesicle-like structures and debris.
Our experiments show that the addition of phage to low honey concentrations is advantageous, and that even fourfold diluted honey combined with phage, presents no loss of antibacterial activity toward E. coli. Portuguese honeys possess excellent antibiofilm activity and may be potential alternative therapeutic agents in biofilm-related wound infection. Furthermore, to our knowledge this is the first study that assessed the impacts of phage-honey combinations in bacterial cells.
The synergistic effect obtained was shown to be promising, since the antiviral effect of honey limits the emergence of phage resistant phenotypes.
Monday, December 25, 2017
Propolis may Help Treat a Sore Throat
The best folk remedy for sore throat
Mice Times
Propolis to dissolve. Propolis, like honey, has healing properties and it can be called one of the best natural antiseptics. If a sore throat, instead of drugstore candy dissolve a piece of propolis several times a day.
Mice Times
Propolis to dissolve. Propolis, like honey, has healing properties and it can be called one of the best natural antiseptics. If a sore throat, instead of drugstore candy dissolve a piece of propolis several times a day.
Sunday, December 24, 2017
Propolis May Help Treat White-nose Syndrome (WNS) in Bats
Evidence for Anti-Pseudogymnoascus destructans (Pd) Activity of Propolis
Antibiotics (Basel). 2017 Dec 21;7(1)
White-nose syndrome (WNS) in bats, caused by Pseudogymnoascus destructans (Pd), is a cutaneous infection that has devastated North American bat populations since 2007. At present, there is no effective method for controlling this disease.
Here, we evaluated the effect of propolis against Pd in vitro. Using Sabouraud dextrose agar (SDA) medium, approximately 1.7 × 10⁷ conidia spores of the Pd strain M3906-2/mL were spread on each plate and grown to form a consistent lawn. A Kirby-Bauer disk diffusion assay was employed using different concentrations of propolis (1%, 2%, 3%, 4%, 5%, 10%, 15%, 20%, 25%), in plates incubated at 8 °C and 15 °C. At 8 °C and 15 °C, as the concentration of propolis increased, there was an increasing zone of inhibition (ZOI), reaching the highest degree at 10% and 25% concentrations, respectively.
A germule suppression assay showed a similar effect on Pd conidia germination. A MALDI-TOF-MS analysis of propolis revealed multiple constituents with a potential anti-Pd activity, including cinnamic acid, p-coumaric acid, and dihydrochalcones, which could be further tested for their individual effects.
Our study suggests that propolis or its individual constituents might be suitable products against Pd.
Saturday, December 23, 2017
Consumption of 'Mad Honey' for Medicinal Purposes Not Recommended
Effect on oxidative stress, hepatic chemical metabolizing parameters, and genotoxic damage of mad honey intake in rats
Hum Exp Toxicol. 2017 Jan 1:960327117745691
A total of 66 male Wistar rats were used and six groups (control: 10 animals and experimental: 12 animals) were formed. While a separate control group was established for each study period, mad honey application to the animals in the experimental group was carried out with a single dose (12.5 g kg-1 body weight (b.w.); acute stage), at a dose of 7.5 g kg-1 b.w. for 21 days (subacute stage), and at a dose of 5 g kg-1 b.w. for 60 days (chronic stage).
Tissue and blood oxidative stress markers (malondialdehyde (MDA), nitric oxide (NO), 4-hydroxynonenal (HNE), superoxide dismutase, catalase, glutathione (GSH) peroxidase, and glucose-6-phosphate dehydrogenase), hepatic chemical metabolizing parameters in the liver (cytochrome P450 2E1, nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase, nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c reductase (CYTC), GSH S-transferase (GST), and GSH), and micronucleus and comet test in some samples were examined.
Findings from the study showed that single and repeated doses given over the period increased MDA, NO, and HNE levels while decreasing/increasing tissue and blood antioxidant enzyme activities. From hepatic chemical metabolizing parameters, GST activity increased in the subacute and chronic stages and CYTC activity increased in the acute period, whereas GSH level decreased in the subacute stage. Changes in tail and head intensities were found in most of the comet results.
Mad honey caused oxidative stresses for each exposure period and made some significant changes on the comet test in certain periods for some samples obtained. In other words, according to the available research results obtained, careless consumption of mad honey for different medical purposes is not appropriate.
Hum Exp Toxicol. 2017 Jan 1:960327117745691
A total of 66 male Wistar rats were used and six groups (control: 10 animals and experimental: 12 animals) were formed. While a separate control group was established for each study period, mad honey application to the animals in the experimental group was carried out with a single dose (12.5 g kg-1 body weight (b.w.); acute stage), at a dose of 7.5 g kg-1 b.w. for 21 days (subacute stage), and at a dose of 5 g kg-1 b.w. for 60 days (chronic stage).
Tissue and blood oxidative stress markers (malondialdehyde (MDA), nitric oxide (NO), 4-hydroxynonenal (HNE), superoxide dismutase, catalase, glutathione (GSH) peroxidase, and glucose-6-phosphate dehydrogenase), hepatic chemical metabolizing parameters in the liver (cytochrome P450 2E1, nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase, nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome c reductase (CYTC), GSH S-transferase (GST), and GSH), and micronucleus and comet test in some samples were examined.
Findings from the study showed that single and repeated doses given over the period increased MDA, NO, and HNE levels while decreasing/increasing tissue and blood antioxidant enzyme activities. From hepatic chemical metabolizing parameters, GST activity increased in the subacute and chronic stages and CYTC activity increased in the acute period, whereas GSH level decreased in the subacute stage. Changes in tail and head intensities were found in most of the comet results.
Mad honey caused oxidative stresses for each exposure period and made some significant changes on the comet test in certain periods for some samples obtained. In other words, according to the available research results obtained, careless consumption of mad honey for different medical purposes is not appropriate.
Friday, December 22, 2017
Biologically Active Components of Bee Venom
Isolation and Identification of Biologically Active Components from the Honey Bee Venom Apis Mellifera L. Caucasica
Medcrave
The venom of the European honey bee Apis mellifera is an intricate mixture of chemical compositions, including proteins, peptides, enzymes, and other small molecules. Lately, there has
been growing interest in the use of melittin, due to its wide range of biological and potential therapeutic applications. Melittin, which is considered to be an antimicrobial, antitumor, and antiinflammatory peptide, is the main component (≥50% (w/w)) of honey bee venom and is widely used in oriental medicine and studied as an alternative for treating drug-resistant infections. In parallel to antimicrobial peptides for therapeutic use in humans, melittin can be used to fight economically important plant pathogens that limit crop production globally. Phospholipase
A2 (PLA2) and hyaluronidase (HYA) are the two major enzymatic proteins present in the bee venom [6,7]. Both of these enzymes are classified as major allergens according to the International
Union of Immunological Societies, as they are capable of inducing
the IgE response in susceptible individuals.
The results of study confirm that a high purity and recovery yield of melittin can be obtained with a one-step purification method with strong cationexchange chromatography resins using
sodium phosphate buffer at pH 6...
Medcrave
The venom of the European honey bee Apis mellifera is an intricate mixture of chemical compositions, including proteins, peptides, enzymes, and other small molecules. Lately, there has
been growing interest in the use of melittin, due to its wide range of biological and potential therapeutic applications. Melittin, which is considered to be an antimicrobial, antitumor, and antiinflammatory peptide, is the main component (≥50% (w/w)) of honey bee venom and is widely used in oriental medicine and studied as an alternative for treating drug-resistant infections. In parallel to antimicrobial peptides for therapeutic use in humans, melittin can be used to fight economically important plant pathogens that limit crop production globally. Phospholipase
A2 (PLA2) and hyaluronidase (HYA) are the two major enzymatic proteins present in the bee venom [6,7]. Both of these enzymes are classified as major allergens according to the International
Union of Immunological Societies, as they are capable of inducing
the IgE response in susceptible individuals.
The results of study confirm that a high purity and recovery yield of melittin can be obtained with a one-step purification method with strong cationexchange chromatography resins using
sodium phosphate buffer at pH 6...
Thursday, December 21, 2017
Propolis Component Helps Treat Benign Prostate Hyperplasia (BPH)
Chrysin attenuates testosterone-induced benign prostate hyperplasia in rats
Food Chem Toxicol. 2017 Dec 14;111:650-659
Benign prostate hyperplasia (BPH) is a common age-related health problem affecting almost 3 out of 4 men in their sixties. Chrysin is a dietary phytoestrogen found naturally in bee propolis and various plant extracts. It possesses antioxidant, anti-inflammatory and anti-proliferative properties. The current study was conducted to explore the role chrysin plays in protection against testosterone-induced BPH in rats. On grounds of a preliminary experiment, a dose of chrysin (50 mg/kg) was chosen for further investigation. Testosterone significantly depleted glutathione, suppressed superoxide dismutase and catalase activities, and elevated lipid peroxidation. Moreover, it markedly scaled down the level of cleaved caspase-3 enzyme, reduced Bax/Bcl-2 ratio and mRNA expression of p53 and p21; conversely, protein expression of proliferating cell nuclear antigen was enhanced. Chrysin alleviated testosterone-induced oxidative stress and restored cleaved caspase-3 level, Bax/Bcl-2 ratio and mRNA expression of p53 and p21 to almost control levels. Chrysin prevented the increase in binding activity of nuclear factor kappa B (NF-κB) p65 subunit, mRNA expression of insulin-like growth factor 1 (IGF-1) and insulin-like growth factor 1 receptor (IGF-1R). These data highlight the protective role of chrysin against experimentally-induced BPH. This is attributed - at least partly - to its antioxidant, antiproliferative and proapoptotic properties.
Food Chem Toxicol. 2017 Dec 14;111:650-659
Wednesday, December 20, 2017
Propolis Helps Treat the Common Cold
Propolis nasal spray effectively improves recovery from infectious acute rhinitis and common cold symptoms in children: a pilot study
J Biol Regul Homeost Agents. 2017 Oct-Dec;31(4):943-950.
Currently, treatment for acute rhinitis (AR) is symptomatic but no clear agreement exists to control its development. Propolis extract may appear as a promising natural treatment for AR, but its beneficial effects have not yet been fully tested. Forty children suffering from AR and common cold symptoms aged between 2-12 years were enrolled in a prospective epidemiological multicentre study.
A 7-day treatment with propolis nasal spray (3 times/day) was applied and a comparison of symptomatology, subjective global improvement and quality of life (QoL) between baseline (day 1) and final (day 7) visits were performed. The main goal was to evaluate the changes in symptom intensity using the Jackson's scoring test. After 7 days of treatment there was a significant decrease of symptoms both in the total score (p less than 0.0001) and in regard to each AR symptom (p less than 0.01). On the whole, the sample reported no symptoms by day 7, and the resolution of symptoms occurred approximately at day 4. Furthermore, there was no need for supplementary treatment. Both the subjective global improvement impression and the QoL of patients appeared to significantly improve after treatment. No adverse events (AEs) were found globally.
It can be concluded that propolis nasal spray effectively improves recovery from infectious AR and common cold symptoms in children and is an optimal alternative in the treatment of this disease without need for any adjuvant treatment.
Tuesday, December 19, 2017
Propolis Component Protects Against Lung Injury
Discovery of caffeic acid phenethyl ester derivatives as novel myeloid differentiation protein 2 inhibitors for treatment of acute lung injury
Eur J Med Chem. 2018 Jan 1;143:361-375
Myeloid differentiation protein 2 (MD2) is an essential molecule which recognizes lipopolysaccharide (LPS), leading to initiation of inflammation through the activation of Toll-like receptor 4 (TLR4) signaling. Caffeic acid phenethyl ester (CAPE) from propolis of honeybee hives could interfere interactions between LPS and the TLR4/MD2 complex, and thereby has promising anti-inflammatory properties. In this study, we designed and synthesized 48 CAPE derivatives and evaluated their anti-inflammatory activities in mouse primary peritoneal macrophages (MPMs) activated by LPS. The most active compound, 10s, was found to bind with MD2 with high affinity, which prevented formation of the LPS/MD2/TLR4 complex. The binding mode of 10s revealed that the major interactions with MD2 were established via two key hydrogen bonds and hydrophobic interactions. Furthermore, 10s showed remarkable protective effects against LPS-caused ALI (acute lung injury) in vivo. Taken together, this work provides new lead structures and candidates as MD2 inhibitors for the development of anti-inflammatory drugs.
Myeloid differentiation protein 2 (MD2) is an essential molecule which recognizes lipopolysaccharide (LPS), leading to initiation of inflammation through the activation of Toll-like receptor 4 (TLR4) signaling. Caffeic acid phenethyl ester (CAPE) from propolis of honeybee hives could interfere interactions between LPS and the TLR4/MD2 complex, and thereby has promising anti-inflammatory properties. In this study, we designed and synthesized 48 CAPE derivatives and evaluated their anti-inflammatory activities in mouse primary peritoneal macrophages (MPMs) activated by LPS. The most active compound, 10s, was found to bind with MD2 with high affinity, which prevented formation of the LPS/MD2/TLR4 complex. The binding mode of 10s revealed that the major interactions with MD2 were established via two key hydrogen bonds and hydrophobic interactions. Furthermore, 10s showed remarkable protective effects against LPS-caused ALI (acute lung injury) in vivo. Taken together, this work provides new lead structures and candidates as MD2 inhibitors for the development of anti-inflammatory drugs.
Monday, December 18, 2017
Antioxidant and Antimicrobial Properties of Chestnut Honey
Antioxidant and antimicrobial properties of Casteanea sativa Miller chestnut honey produced on Mount Etna (Sicily)
Nat Prod Res. 2017 Dec 13:1-8
The aim of this study was the evaluation of antibacterial and antioxidant properties of Monofloral Etna Castanea sativa Miller honeys. Escherichia coli ATCC 25,922, Pseudomonas aeruginosa ATCC 27,853, Enterococcus faecalis ATCC 29,211 and Staphylococcus aureus ATCC 29,213 were investigated for their susceptibilities to two different honeys. Antioxidant activity was evaluated by ORAC, NO scavenger assays, FRAP and DPPH.
Antioxidant activity and antibacterial properties were compared with chestnut honeys from different geographical areas and with Manuka honey. UPLC-MS/MS was used for major components characterisation.
Sunday, December 17, 2017
Brazilian Stingless Bee Propolis High in Phenols, Flavonoids, Antioxidants
Antioxidant Activity of a Geopropolis from Northeast Brazil: Chemical Characterization and Likely Botanical Origin
Evid Based Complement Alternat Med. 2017;2017:4024721
Geopropolis is a product containing wax, plant resin, and soil particles. It is elaborated by stingless bees of tribe Meliponini. Methanol extracts of sample of geopropolis produced by Scaptotrigona postica ("mandaguari") in the state of Rio Grande do Norte (RN, northeast Brazil) were analyzed for the determination of standard parameters (total phenols, total flavonoids, and radical scavenging activity) and chemical characterization by HPLC-DAD-MS/MS analysis.
The sample analyzed has high contents of total phenols and flavonoids, as well as high antioxidant activity. The constituents characterized were mainly flavonols, such as quercetin methyl ethers, and methoxychalcones. Such chemical profile is similar to the composition of a green propolis from the same area of RN, which is produced by Africanized Apis mellifera, using shoot apices of Mimosa tenuiflora, popularly known as "jurema-preta."
This finding provides evidence that "mandaguari" geopropolis and honeybee propolis have the same botanical origin in RN. The sharing of a plant resin source by phylogenetically distant bees (Apinae and Meliponinae) suggests that bee genetic factors play little role in the choice of plants for resin collection and that the availability of potential botanical sources plays a decisive role.
Evid Based Complement Alternat Med. 2017;2017:4024721
Geopropolis is a product containing wax, plant resin, and soil particles. It is elaborated by stingless bees of tribe Meliponini. Methanol extracts of sample of geopropolis produced by Scaptotrigona postica ("mandaguari") in the state of Rio Grande do Norte (RN, northeast Brazil) were analyzed for the determination of standard parameters (total phenols, total flavonoids, and radical scavenging activity) and chemical characterization by HPLC-DAD-MS/MS analysis.
The sample analyzed has high contents of total phenols and flavonoids, as well as high antioxidant activity. The constituents characterized were mainly flavonols, such as quercetin methyl ethers, and methoxychalcones. Such chemical profile is similar to the composition of a green propolis from the same area of RN, which is produced by Africanized Apis mellifera, using shoot apices of Mimosa tenuiflora, popularly known as "jurema-preta."
This finding provides evidence that "mandaguari" geopropolis and honeybee propolis have the same botanical origin in RN. The sharing of a plant resin source by phylogenetically distant bees (Apinae and Meliponinae) suggests that bee genetic factors play little role in the choice of plants for resin collection and that the availability of potential botanical sources plays a decisive role.
Saturday, December 16, 2017
Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens
In vitro and in vivo activity of Manuka honey against NDM-1-producing Klebsiella pneumoniae ST11
Future Microbiol. 2018 Jan;13:13-26
AIM:
To determine the therapeutic potential of Manuka honey against New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae ST11 in vitro and in vivo.
MATERIALS & METHODS:
Carbapenamases and metallo-β-lactamases-producing K. pneumoniae ST11 isolated from blood culture was confirmed by VITEK-2® system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing, followed by determination of minimum inhibitory concentration (μg/ml) using VITEK-2 system. Genetic analysis of bla NDM-1 was done by PCR, pulsed-field gel electrophoresis and DNA hybridization. In vitro and in vivo efficacy of Manuka honey was performed by microbroth dilution assay and BALB/c mice model respectively.
RESULTS:
K. pneumoniae ST11 displayed resistance to commonly used antibiotics. bla NDM-1 was located on 150 and 270kb plasmids. Minimum inhibitory concentration and minimum bactericidal concentration of Manuka honey was 30% (v/v) and substantial reduction of bacterial mean log value (>1 log) was observed in mice. Histological analysis of mice liver and kidneys demonstrated mild to moderate inflammation.
CONCLUSION:
Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens.
Future Microbiol. 2018 Jan;13:13-26
AIM:
To determine the therapeutic potential of Manuka honey against New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae ST11 in vitro and in vivo.
MATERIALS & METHODS:
Carbapenamases and metallo-β-lactamases-producing K. pneumoniae ST11 isolated from blood culture was confirmed by VITEK-2® system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing, followed by determination of minimum inhibitory concentration (μg/ml) using VITEK-2 system. Genetic analysis of bla NDM-1 was done by PCR, pulsed-field gel electrophoresis and DNA hybridization. In vitro and in vivo efficacy of Manuka honey was performed by microbroth dilution assay and BALB/c mice model respectively.
RESULTS:
K. pneumoniae ST11 displayed resistance to commonly used antibiotics. bla NDM-1 was located on 150 and 270kb plasmids. Minimum inhibitory concentration and minimum bactericidal concentration of Manuka honey was 30% (v/v) and substantial reduction of bacterial mean log value (>1 log) was observed in mice. Histological analysis of mice liver and kidneys demonstrated mild to moderate inflammation.
CONCLUSION:
Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens.
Friday, December 15, 2017
Bee Venom Used to Treat Arthritis
Bee venom used to treat arthritis in Norwich woman
NORWICH, Kan. (KAKE) - A Norwich, Kansas woman has a unique approach to treating her arthritis by using bee venom as a medication.
Sharon Rowan says she suffers from debilitating arthritis in her hands and one day, she had enough and knew she needed a new treatment.
“It was enough that the joints would just throb. It hurt to write, it hurt to do anything,” Rowan said. “That is when it’s time to think about it, its time to minimize this pain.”
She had friends that used bee venom as a treatment for arthritis and she gave it a try. Rowan has been an avid beekeeper for the past 50 years.
“I put a bee down there on it and it worked, because it quit hurting,” Rowan said of her hand pain from arthritis. “I talked to my doctor about it and he said it works as good as my shot will so go for it.”
Thursday, December 14, 2017
'Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens'
In vitro and in vivo activity of Manuka honey against NDM-1-producing Klebsiella pneumoniae ST11
Future Microbiol. 2017 Dec 11
AIM:
To determine the therapeutic potential of Manuka honey against New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae ST11 in vitro and in vivo.
MATERIALS & METHODS:
Carbapenamases and metallo-β-lactamases-producing K. pneumoniae ST11 isolated from blood culture was confirmed by VITEK-2® system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing, followed by determination of minimum inhibitory concentration (μg/ml) using VITEK-2 system. Genetic analysis of bla NDM-1 was done by PCR, pulsed-field gel electrophoresis and DNA hybridization. In vitro and in vivo efficacy of Manuka honey was performed by microbroth dilution assay and BALB/c mice model respectively.
RESULTS:
K. pneumoniae ST11 displayed resistance to commonly used antibiotics. bla NDM-1 was located on 150 and 270kb plasmids. Minimum inhibitory concentration and minimum bactericidal concentration of Manuka honey was 30% (v/v) and substantial reduction of bacterial mean log value (>1 log) was observed in mice. Histological analysis of mice liver and kidneys demonstrated mild to moderate inflammation.
CONCLUSION:
Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens.
Future Microbiol. 2017 Dec 11
AIM:
To determine the therapeutic potential of Manuka honey against New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae ST11 in vitro and in vivo.
MATERIALS & METHODS:
Carbapenamases and metallo-β-lactamases-producing K. pneumoniae ST11 isolated from blood culture was confirmed by VITEK-2® system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing, followed by determination of minimum inhibitory concentration (μg/ml) using VITEK-2 system. Genetic analysis of bla NDM-1 was done by PCR, pulsed-field gel electrophoresis and DNA hybridization. In vitro and in vivo efficacy of Manuka honey was performed by microbroth dilution assay and BALB/c mice model respectively.
RESULTS:
K. pneumoniae ST11 displayed resistance to commonly used antibiotics. bla NDM-1 was located on 150 and 270kb plasmids. Minimum inhibitory concentration and minimum bactericidal concentration of Manuka honey was 30% (v/v) and substantial reduction of bacterial mean log value (>1 log) was observed in mice. Histological analysis of mice liver and kidneys demonstrated mild to moderate inflammation.
CONCLUSION:
Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens.
Wednesday, December 13, 2017
New Zealand Trademarks Manuka Honey
NZ trademarks mānuka honey and officials create new definition
Many New Zealand beekeepers are celebrating the sweet taste of a landmark decision by United Kingdom authorities to accept the term mānuka honey for trade marking.
The decision reached overnight by the United Kingdom Trade Mark Registry recognising mānuka honey as a certification mark means the New Zealand beekeeping industry can seek further protection of the term.
At the same time Agriculture Minister Damien O'Connor announced the Ministry for Primary Industries had produced a definition for mānuka honey.
He said the decision would not please all producers, but would safeguard the industry from cowboy operators and protect New Zealand's trade reputation.
"The scale of the problem was never truly identified but what we know is there was more manuka honey sold than was being produced, so obviously some cowboy operators were mixing honey. That's not good for our reputation or for customers," O'Connor said...
Tuesday, December 12, 2017
Cytotoxic Effect of Biologically-Transformed Propolis
Biologically Transformed Propolis Exhibits Cytotoxic Effect on A375 Malignant Melanoma Cells In Vitro
Proceedings 2017, 1(10), 1059
Propolis has been used for its health benefits, due to high phenolic content. Recently it has been shown that the extraction methods which yielded phenolic molecules, affected the anti-oxidant and anticancer effect of propolis.
In our previous study we showed that biotransformation of propolis via Lactobacillus plantarum might increase antioxidative effect. In this study, we investigated the cytotoxic effect of this propolis sample on A375 melanoma cells. The propolis samples were extracted in water. The phenolic molecules were determined with LC MS/MS. Cytotoxicity was evaluated by means of the WST.
Water-extracted propolis samples were incubated with L. plantarum (1.5%) in 37 °C for 24 h. A375 cells were treated by propolis with doses of from 25 to 1000 µg/mL, for periods of 24 h, 48 h and 72 h. Cytotoxicity MTT tests were performed. The significantly high phenolic compounds mainly; Quercetine (514 ng/mL), rutin (623 ng/mL), ellagic acid (331 ng/mL), epicatechin (125 ng/mL) were found in propolis samples IC50 values were 412.5 µg/mL (24 h) and 314 µg/mL (48 h) and 353 µg/mL (72 h).
In conclusion, our data showed that the cytotoxic effect of biologically transformed propolis which have high content of rutin, quercetin, ellagic acid, epicatechin. Biotransformation might be a useful strategy to increase bioavailability of phenolic molecules in propolis.
Proceedings 2017, 1(10), 1059
Propolis has been used for its health benefits, due to high phenolic content. Recently it has been shown that the extraction methods which yielded phenolic molecules, affected the anti-oxidant and anticancer effect of propolis.
In our previous study we showed that biotransformation of propolis via Lactobacillus plantarum might increase antioxidative effect. In this study, we investigated the cytotoxic effect of this propolis sample on A375 melanoma cells. The propolis samples were extracted in water. The phenolic molecules were determined with LC MS/MS. Cytotoxicity was evaluated by means of the WST.
Water-extracted propolis samples were incubated with L. plantarum (1.5%) in 37 °C for 24 h. A375 cells were treated by propolis with doses of from 25 to 1000 µg/mL, for periods of 24 h, 48 h and 72 h. Cytotoxicity MTT tests were performed. The significantly high phenolic compounds mainly; Quercetine (514 ng/mL), rutin (623 ng/mL), ellagic acid (331 ng/mL), epicatechin (125 ng/mL) were found in propolis samples IC50 values were 412.5 µg/mL (24 h) and 314 µg/mL (48 h) and 353 µg/mL (72 h).
In conclusion, our data showed that the cytotoxic effect of biologically transformed propolis which have high content of rutin, quercetin, ellagic acid, epicatechin. Biotransformation might be a useful strategy to increase bioavailability of phenolic molecules in propolis.
Monday, December 11, 2017
Royal Jelly May May Be a Treatment for Alzheimer’s Disease, Colitis, Crohn’s Disease, Menopausal Symptoms, Diabetes, Osteoporosis
10 Health Benefits of Royal Jelly and Why It May Be A Treatment for Alzheimer’s Disease
By Kaitlin Covel Dec 9 2017
Royal Jelly is not the magic pill cure-all of the natural world, but the incredible health benefits do give it an almost miraculous reputation considering that scientific studies testify to its potential to positively treat a variety of illnesses and symptoms including that of Alzheimer’s Disease.
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What is royal jelly you ask? Royal jelly is the substance milked from the jaws of nurse bees in the hive exclusively for the queen bee. Royal jelly contains significant amounts of protein that are important for cell growth and reproduction as well as carbohydrates, fats, free amino acids, vitamins and minerals. It is interesting to note that royal jelly is rich in B vitamins, the 8 amino acids humans cannot produce, and potassium, magnesium, calcium, copper, sulfur, zinc, iron and manganese as well. No wonder royal jelly is considered a wellspring of nutrients. Here are the top 10 health benefits of royal jelly:
1. Alzheimer’s Disease Treatment
Research conducted recently demonstrated that royal jelly benefits neural function which indicates that it may improve short-term memory for patients with Alzheimer’s disease. The study was published in Advanced Biomedical Research. The powerful antioxidants in Royal Jelly make it an effective and natural treatment for fighting free radical attacks and oxidative stress which occur in many neurodegenerative diseases like Alzheimer’s disease.
2. Immune System Modulator
According to research published in a 2001 study in the journal International Immunopharmacology, royal jelly can boost the functions of the immune system. Histamine responses to allergens were suppressed immediately, evidencing that it may help to relieve allergies of a seasonal nature. Hopefully more research will be completed to validate royal jelly’s usefulness in treating allergies.
3. Colitis, Crohn’s Disease, and IBS Treatment
A European study found that royal jelly protected the mucosa of the colon by limiting the negative effects of the acidic substance that was given to the rats with colitis. Another finding in the study was that royal jelly protected against erosion of the cells in the colon. To replicate the results of this study in humans, the researchers concluded that a 140 pound person would need to take approximately 10 grams of royal jelly per day. In order to get this amount, a freeze-dried dose of royal jelly would be about 2 teaspoons and a fresh dose would be about 2 tablespoons....
Sunday, December 10, 2017
Hydrophilic Bioactive Components of Greek Royal Jelly
Targeted profiling of hydrophilic constituents of royal jelly by hydrophilic interaction liquid chromatography-tandem mass spectrometry
J Chromatogr A. 2017 Nov 13. pii: S0021-9673(17)31667-9
In the present work a Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry (HILIC-MS/MS) method was developed for the efficient separation and quantification of a large number of small polar bioactive molecules in Royal Jelly.
The method was validated and provided satisfactory detection sensitivity for 88 components. Quantification was proven to be precise for 64 components exhibiting good linearity, recoveries R% > 90% for the majority of analytes and intra- and inter-day precision from 0.14 to 20% RSD.
Analysis of 125 fresh royal jelly samples of Greek origin provided useful information on royal jelly's components revealing lysine, ribose, proline, melezitose and glutamic acid to be in high abundance. In addition the occurrence of 18 hydrophilic nutrients which have not been reported previously as royal jelly constituents is shown.
J Chromatogr A. 2017 Nov 13. pii: S0021-9673(17)31667-9
In the present work a Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry (HILIC-MS/MS) method was developed for the efficient separation and quantification of a large number of small polar bioactive molecules in Royal Jelly.
The method was validated and provided satisfactory detection sensitivity for 88 components. Quantification was proven to be precise for 64 components exhibiting good linearity, recoveries R% > 90% for the majority of analytes and intra- and inter-day precision from 0.14 to 20% RSD.
Analysis of 125 fresh royal jelly samples of Greek origin provided useful information on royal jelly's components revealing lysine, ribose, proline, melezitose and glutamic acid to be in high abundance. In addition the occurrence of 18 hydrophilic nutrients which have not been reported previously as royal jelly constituents is shown.
Saturday, December 09, 2017
Indian Propolis Shows Anti-Cancer Effect
Standardization, chemical profiling, in vitro cytotoxic effects, in vivo anti-carcinogenic potential and biosafety profile of Indian propolis
J Ayurveda Integr Med. 2017 Dec 4. pii: S0975-9476(17)30185-7
BACKGROUND:
Propolis from apiculture is known for wide range of medicinal properties owing to its vast chemical constituents including polyphenols, flavonoids and anticancer agent Caffeic acid phenethyl ester (CAPE).
OBJECTIVES:
The objective of the study was to extract and standardize Indian propolis (IP) with respect to selected markers by newly developed High performance liquid chromatography (HPLC) method, to evaluate in vitro and in vivo anticancer activity and biosafety of Indian propolis.
MATERIALS AND METHODS:
IP was extracted, optimized and standardized using a newly developed and validated HPLC method for simultaneous estimation of caffeic acid, apigenin, quercetin and CAPE. The standardised ethanolic extract of IP (EEIP) was screened for in vitro cytotoxicity using sulforhodamine B (SRB) assay, in vivo anti-carcinogenic effect against Dalton's Lymphoma ascites (DLA) cells, hemolytic effect and pesticide analysis.
RESULTS:
The EEIP was found to contain more amount of total flavonoids (23.61+ 0.0452 mg equivalent of quercetin/g), total polyphenolics (34.82 + 0.0785 mg equivalent of gallic acid/g) and all selected markers except caffeic acid compared to all other extracts. EEIP showed better anti-cancer potential than CAPE on MCF-7 and HT-29 cell line and significant (p < 0.01) in vivo anti-carcinogenic effects against DLA in comparison with 5-fluorouracil. EEIP was found to be non-hemolytic.
CONCLUSION:
From in vitro cytotoxicity, in vivo anti-carcinogenicity and biosafety studies it can be concluded that the standardized EEIP is safe and can be considered for further development as a biomedicine.
J Ayurveda Integr Med. 2017 Dec 4. pii: S0975-9476(17)30185-7
BACKGROUND:
Propolis from apiculture is known for wide range of medicinal properties owing to its vast chemical constituents including polyphenols, flavonoids and anticancer agent Caffeic acid phenethyl ester (CAPE).
OBJECTIVES:
The objective of the study was to extract and standardize Indian propolis (IP) with respect to selected markers by newly developed High performance liquid chromatography (HPLC) method, to evaluate in vitro and in vivo anticancer activity and biosafety of Indian propolis.
MATERIALS AND METHODS:
IP was extracted, optimized and standardized using a newly developed and validated HPLC method for simultaneous estimation of caffeic acid, apigenin, quercetin and CAPE. The standardised ethanolic extract of IP (EEIP) was screened for in vitro cytotoxicity using sulforhodamine B (SRB) assay, in vivo anti-carcinogenic effect against Dalton's Lymphoma ascites (DLA) cells, hemolytic effect and pesticide analysis.
RESULTS:
The EEIP was found to contain more amount of total flavonoids (23.61+ 0.0452 mg equivalent of quercetin/g), total polyphenolics (34.82 + 0.0785 mg equivalent of gallic acid/g) and all selected markers except caffeic acid compared to all other extracts. EEIP showed better anti-cancer potential than CAPE on MCF-7 and HT-29 cell line and significant (p < 0.01) in vivo anti-carcinogenic effects against DLA in comparison with 5-fluorouracil. EEIP was found to be non-hemolytic.
CONCLUSION:
From in vitro cytotoxicity, in vivo anti-carcinogenicity and biosafety studies it can be concluded that the standardized EEIP is safe and can be considered for further development as a biomedicine.
Friday, December 08, 2017
Manuka Honey Helps Treat Severe Atopic Eczematous Dermatitis
Manuka Honey: A Case Study of Severe Atopic Eczematous Dermatitis Reaction to Henna Tattoo
Plast Surg Nurs. 2017 Oct/Dec;37(4):154-157
Many mainstream medications were derived from plants and originally utilized in patient management well prior to the extensive research and testing processes of current pharmaceutical standards. The evolution of therapeutic management within the pharmaceutical and skin care industry often uses synthetic processing of products with less of a focus on the natural ingredients from which they were originally derived.
However, more recently there has been a shift in pharmacological management to include the therapeutic use of more holistic medicines and practices and thus a broadening of the uses of nontraditional medical treatment options. This has been seen in the use of treatments, such as Manuka honey, for skin conditions and dermal injuries. It is often with off-label uses, or conditions resistant to other treatments, that then prompt the use of holistic products and the true value of the product is validated.
As with the following case study, the example of the use of Manuka honey on a severe atopic contact dermatitis eczematous reaction provides further documentation and supportive evidence of the potential efficacy of the properties of this particular natural product.
Plast Surg Nurs. 2017 Oct/Dec;37(4):154-157
Many mainstream medications were derived from plants and originally utilized in patient management well prior to the extensive research and testing processes of current pharmaceutical standards. The evolution of therapeutic management within the pharmaceutical and skin care industry often uses synthetic processing of products with less of a focus on the natural ingredients from which they were originally derived.
However, more recently there has been a shift in pharmacological management to include the therapeutic use of more holistic medicines and practices and thus a broadening of the uses of nontraditional medical treatment options. This has been seen in the use of treatments, such as Manuka honey, for skin conditions and dermal injuries. It is often with off-label uses, or conditions resistant to other treatments, that then prompt the use of holistic products and the true value of the product is validated.
As with the following case study, the example of the use of Manuka honey on a severe atopic contact dermatitis eczematous reaction provides further documentation and supportive evidence of the potential efficacy of the properties of this particular natural product.
Thursday, December 07, 2017
Propolis Component Useful in Treatment of Acute Lung Injury
Discovery of caffeic acid phenethyl ester derivatives as novel myeloid differentiation protein 2 inhibitors for treatment of acute lung injury
Eur J Med Chem. 2017 Dec 1;143:361-375
Myeloid differentiation protein 2 (MD2) is an essential molecule which recognizes lipopolysaccharide (LPS), leading to initiation of inflammation through the activation of Toll-like receptor 4 (TLR4) signaling. Caffeic acid phenethyl ester (CAPE) from propolis of honeybee hives could interfere interactions between LPS and the TLR4/MD2 complex, and thereby has promising anti-inflammatory properties.
In this study, we designed and synthesized 48 CAPE derivatives and evaluated their anti-inflammatory activities in mouse primary peritoneal macrophages (MPMs) activated by LPS. The most active compound, 10s, was found to bind with MD2 with high affinity, which prevented formation of the LPS/MD2/TLR4 complex. The binding mode of 10s revealed that the major interactions with MD2 were established via two key hydrogen bonds and hydrophobic interactions. Furthermore, 10s showed remarkable protective effects against LPS-caused ALI (acute lung injury) in vivo.
Taken together, this work provides new lead structures and candidates as MD2 inhibitors for the development of anti-inflammatory drugs.
Eur J Med Chem. 2017 Dec 1;143:361-375
Myeloid differentiation protein 2 (MD2) is an essential molecule which recognizes lipopolysaccharide (LPS), leading to initiation of inflammation through the activation of Toll-like receptor 4 (TLR4) signaling. Caffeic acid phenethyl ester (CAPE) from propolis of honeybee hives could interfere interactions between LPS and the TLR4/MD2 complex, and thereby has promising anti-inflammatory properties.
In this study, we designed and synthesized 48 CAPE derivatives and evaluated their anti-inflammatory activities in mouse primary peritoneal macrophages (MPMs) activated by LPS. The most active compound, 10s, was found to bind with MD2 with high affinity, which prevented formation of the LPS/MD2/TLR4 complex. The binding mode of 10s revealed that the major interactions with MD2 were established via two key hydrogen bonds and hydrophobic interactions. Furthermore, 10s showed remarkable protective effects against LPS-caused ALI (acute lung injury) in vivo.
Taken together, this work provides new lead structures and candidates as MD2 inhibitors for the development of anti-inflammatory drugs.
Wednesday, December 06, 2017
Honey Effective as Treatment for Chemotherapy-Induced Mucositis in Paediatric Oncology Patients
Global Health Journal Club: Is Honey Effective as a Treatment for Chemotherapy-induced Mucositis in Paediatric Oncology Patients?
J Trop Pediatr. 2017 Nov 30
Oral mucositis (OM) is an inflammatory response of mucosal epithelium to the cytotoxic effects of chemotherapy and radiotherapy causing severe oral pain and ulceration, which may complicate the management of cancer. The Mucositis Prevention Guideline Development Group has developed an international guideline for the prevention of mucositis in children receiving treatment for cancer or undergoing haematopoietic stem cell transplantation.
Evidence-based preventative strategies include cryotherapy, low-level light therapy and keratinocyte growth factor. However, these strategies are often not available in resource-poor settings. There is some evidence that honey may be a suitable treatment for OM in adult patients. We performed a literature search of 11 databases to find papers exploring the use of honey to treat chemotherapy-associated mucositis in paediatric oncology patients.
We found four papers, which provide Grade C evidence that honey is effective as a preventative and therapeutic measure for OM in paediatric oncology patients.
J Trop Pediatr. 2017 Nov 30
Oral mucositis (OM) is an inflammatory response of mucosal epithelium to the cytotoxic effects of chemotherapy and radiotherapy causing severe oral pain and ulceration, which may complicate the management of cancer. The Mucositis Prevention Guideline Development Group has developed an international guideline for the prevention of mucositis in children receiving treatment for cancer or undergoing haematopoietic stem cell transplantation.
Evidence-based preventative strategies include cryotherapy, low-level light therapy and keratinocyte growth factor. However, these strategies are often not available in resource-poor settings. There is some evidence that honey may be a suitable treatment for OM in adult patients. We performed a literature search of 11 databases to find papers exploring the use of honey to treat chemotherapy-associated mucositis in paediatric oncology patients.
We found four papers, which provide Grade C evidence that honey is effective as a preventative and therapeutic measure for OM in paediatric oncology patients.
Monday, December 04, 2017
Give Children Honey and Lemon, Not Cough Medicine Says Top Doc
By Henry Bodkin, 1 December 2017
Children should not be given cough medicine but should instead be treated with “old fashioned” honey and lemon, a leading paediatrician has said.
Using over-the-counter syrups and medications risk unintentionally overdosing toddlers and causing “toxic” events, according to Dr Oliver Bevington, from the Royal College of Paediatrics and Child Health.
Chair of the college’s trainees’ committee, Dr Bovington said there was no evidence cough medicines work and added they can end “actually end up doing more harm than good”.
Most childhood coughs and colds get better simply with rest, fluids and possibly paracetamol or ibuprofen, he said.
The intervention follows an announcement by the NHS earlier this year saying it would no longer fund free cough mixture.
“A lot of cough and cold medicines contain active ingredients such as nasal decongestants, antihistamines and cough suppressors that may, in large doses, have adverse effects or be toxic if consumed in large quantities, particularly to the under-sixes who are much more susceptible,” he said.
“The bottom line is there is absolutely no evidence that cough medicines work as there has been very little research with regards to their use and, potentially, they could actually do children more harm than good.
“My advice for parents would be to stick to old fashioned honey and lemon.”...
Children should not be given cough medicine but should instead be treated with “old fashioned” honey and lemon, a leading paediatrician has said.
Using over-the-counter syrups and medications risk unintentionally overdosing toddlers and causing “toxic” events, according to Dr Oliver Bevington, from the Royal College of Paediatrics and Child Health.
Chair of the college’s trainees’ committee, Dr Bovington said there was no evidence cough medicines work and added they can end “actually end up doing more harm than good”.
Most childhood coughs and colds get better simply with rest, fluids and possibly paracetamol or ibuprofen, he said.
The intervention follows an announcement by the NHS earlier this year saying it would no longer fund free cough mixture.
“A lot of cough and cold medicines contain active ingredients such as nasal decongestants, antihistamines and cough suppressors that may, in large doses, have adverse effects or be toxic if consumed in large quantities, particularly to the under-sixes who are much more susceptible,” he said.
“The bottom line is there is absolutely no evidence that cough medicines work as there has been very little research with regards to their use and, potentially, they could actually do children more harm than good.
“My advice for parents would be to stick to old fashioned honey and lemon.”...
Sunday, December 03, 2017
Bee Venom Component Inhibits Tumor Growth
The anti-hepatocellular carcinoma activity of Mel-P15 is mediated by natural killer cells
Oncol Lett. 2017 Dec;14(6):6901-6906
Mel-P15 is a peptide derived from melittin, the main toxic component in the venom of the European honeybee Apis mellifera.
In the present study, the antitumor effects of Mel-P15 and the underlying molecular mechanisms of these effects in vivo were investigated. Mel-P15 directly stimulated natural killer (NK) cell cytotoxicity in vitro, which was increased to 55.45% at a 4 µg/ml dose of Mel-P15. In the mouse liver cancer (H22) xenograft mice model, Mel-P15 suppressed tumor growth in vivo; the tumor inhibitory rate was 61.15% following treatment with 2 mg/kg Mel-P15. In addition, the immune response was activated following Mel-P15 treatment. Mel-P15 treatment increased the spleen and thymus indices, promoted splenocyte proliferation, stimulated NK cytotoxicity and upregulated the secretion of cytokines, including interleukin-2, interferon-γ and tumor necrosis factor-α. In addition, the tumor inhibitory effect of Mel-P15 on BEL-7402-bearing nude mice was abrogated by the selective depletion of NK cells via the intraperitoneal injection of an anti-asialo GM-1 antibody.
The results suggest that Mel-P15 inhibits tumor growth in vivo by promoting NK cell cytotoxicity. Mel-P15 may therefore be a potential immunotherapy candidate for the treatment of hepatocellular carcinoma.
Oncol Lett. 2017 Dec;14(6):6901-6906
Mel-P15 is a peptide derived from melittin, the main toxic component in the venom of the European honeybee Apis mellifera.
In the present study, the antitumor effects of Mel-P15 and the underlying molecular mechanisms of these effects in vivo were investigated. Mel-P15 directly stimulated natural killer (NK) cell cytotoxicity in vitro, which was increased to 55.45% at a 4 µg/ml dose of Mel-P15. In the mouse liver cancer (H22) xenograft mice model, Mel-P15 suppressed tumor growth in vivo; the tumor inhibitory rate was 61.15% following treatment with 2 mg/kg Mel-P15. In addition, the immune response was activated following Mel-P15 treatment. Mel-P15 treatment increased the spleen and thymus indices, promoted splenocyte proliferation, stimulated NK cytotoxicity and upregulated the secretion of cytokines, including interleukin-2, interferon-γ and tumor necrosis factor-α. In addition, the tumor inhibitory effect of Mel-P15 on BEL-7402-bearing nude mice was abrogated by the selective depletion of NK cells via the intraperitoneal injection of an anti-asialo GM-1 antibody.
The results suggest that Mel-P15 inhibits tumor growth in vivo by promoting NK cell cytotoxicity. Mel-P15 may therefore be a potential immunotherapy candidate for the treatment of hepatocellular carcinoma.
Saturday, December 02, 2017
Bee Bread Shows Antioxidant Activity and Protective Effect
Antioxidant activity and protective effect of bee bread (honey and pollen) in aluminum-induced anemia, elevation of inflammatory makers and hepato-renal toxicity
J Food Sci Technol. 2017 Dec;54(13):4205-4212
Aluminum toxicity might be related to oxidative stress, and the antioxidant activity and protective effect of bee bread, which contains pollen, honey and bees' enzymes, on aluminum induced blood and hepato-renal toxicity was investigated in rats.
Chemical analysis and antioxidant capacity of bee bread were conducted. The animal experiment in rats included; group 1: received distilled water (10 ml/kg b.wt), group 2: received aluminum chloride (662.2 mg/kg b.wt), group 3: received aluminum chloride (662.2 mg/kg b.wt) and ethanolic extract of the bee bread (500 mg/kg b.wt), and group 4: received aluminum chloride (662.2 mg/kg b.wt) and ethanolic extract of the bee bread (750 mg/kg b.wt). Doses were given once daily via a gavage. C-reactive protein, transaminases, urea, creatinine, creatinine clearance, sodium and potassium and urine sodium and potassium were determined on day 28 of the experiment.
Bee bread contained protein, fat, fiber, ash, carbohydrate, phenol and flavonoids and it exhibited antioxidant activity. Aluminum caused a significant elevation of blood urea, transaminase, C-reactive protein and monocyte count and significantly decreased hemoglobin. These changes were significantly ameliorated by the use of bee bread.
Bee bread has an antioxidant property, and exhibited a protective effect on aluminum induced blood and hepato-renal toxicity and elevation of inflammatory markers C-reactive protein, leukocyte and monocyte counts.
J Food Sci Technol. 2017 Dec;54(13):4205-4212
Aluminum toxicity might be related to oxidative stress, and the antioxidant activity and protective effect of bee bread, which contains pollen, honey and bees' enzymes, on aluminum induced blood and hepato-renal toxicity was investigated in rats.
Chemical analysis and antioxidant capacity of bee bread were conducted. The animal experiment in rats included; group 1: received distilled water (10 ml/kg b.wt), group 2: received aluminum chloride (662.2 mg/kg b.wt), group 3: received aluminum chloride (662.2 mg/kg b.wt) and ethanolic extract of the bee bread (500 mg/kg b.wt), and group 4: received aluminum chloride (662.2 mg/kg b.wt) and ethanolic extract of the bee bread (750 mg/kg b.wt). Doses were given once daily via a gavage. C-reactive protein, transaminases, urea, creatinine, creatinine clearance, sodium and potassium and urine sodium and potassium were determined on day 28 of the experiment.
Bee bread contained protein, fat, fiber, ash, carbohydrate, phenol and flavonoids and it exhibited antioxidant activity. Aluminum caused a significant elevation of blood urea, transaminase, C-reactive protein and monocyte count and significantly decreased hemoglobin. These changes were significantly ameliorated by the use of bee bread.
Bee bread has an antioxidant property, and exhibited a protective effect on aluminum induced blood and hepato-renal toxicity and elevation of inflammatory markers C-reactive protein, leukocyte and monocyte counts.
Friday, December 01, 2017
Honey Has Significant Diuretic Activity Alone or in Combination with Propolis
Antioxidant and diuretic activity of co-administration of Capparis spinosa honey and propolis in comparison to furosemide
Asian Pac J Trop Med. 2017 Oct;10(10):974-980
OBJECTIVE:
To study the antioxidant properties of Capparis spinosa (C. spinosa) honey and propolis and the effect of combined honey and propolis administration on urine volume and electrolytes in rats.
METHODS:
C. spinosa honey [1000 mg/kg body weight (b.wt)], propolis (100 mg/kg b.wt), honey/propolis mixture (C. spinosa honey 1000 mg/kg b.wt/ propolis extract 100 mg/kg b.wt ), distilled water (1 mL/kg b.wt) and furosemide (10 mg/kg b.wt) were orally administered to five groups of rats for 21 d. Urine volume, blood and urine sodium, potassium and chloride were measured. The antioxidant activity of propolis and honey was assessed and their total phenols and flavonoids were determined.
RESULTS:
Propolis and C. spinosa honey contain polyphenols including flavonoids and propolis demonstrated higher antioxidant activities than honey. Honey significantly increased urine volume and urine electrolyte excretion. Propolis had no significant effect on urine volume, but co-administration of propolis and honey caused significant diuresis. No major changes were observed in plasma electrolytes with the use of honey, propolis or their combination.
CONCLUSIONS:
Honey and propolis have antioxidant activity and contain polyphenols including flavonoids that are more pronounced in propolis. Honey has a significant diuretic activity alone or in combination with propolis. This is the first study comparing the diuretic effect of co-administration of propolis and C. spinosa honey with furosemide.
Asian Pac J Trop Med. 2017 Oct;10(10):974-980
OBJECTIVE:
To study the antioxidant properties of Capparis spinosa (C. spinosa) honey and propolis and the effect of combined honey and propolis administration on urine volume and electrolytes in rats.
METHODS:
C. spinosa honey [1000 mg/kg body weight (b.wt)], propolis (100 mg/kg b.wt), honey/propolis mixture (C. spinosa honey 1000 mg/kg b.wt/ propolis extract 100 mg/kg b.wt ), distilled water (1 mL/kg b.wt) and furosemide (10 mg/kg b.wt) were orally administered to five groups of rats for 21 d. Urine volume, blood and urine sodium, potassium and chloride were measured. The antioxidant activity of propolis and honey was assessed and their total phenols and flavonoids were determined.
RESULTS:
Propolis and C. spinosa honey contain polyphenols including flavonoids and propolis demonstrated higher antioxidant activities than honey. Honey significantly increased urine volume and urine electrolyte excretion. Propolis had no significant effect on urine volume, but co-administration of propolis and honey caused significant diuresis. No major changes were observed in plasma electrolytes with the use of honey, propolis or their combination.
CONCLUSIONS:
Honey and propolis have antioxidant activity and contain polyphenols including flavonoids that are more pronounced in propolis. Honey has a significant diuretic activity alone or in combination with propolis. This is the first study comparing the diuretic effect of co-administration of propolis and C. spinosa honey with furosemide.
Thursday, November 30, 2017
Beeswax Component May Help Treat Ulcerative Colitis
[Effects in rats of bee-wax alcohols (D-002) on ulcerative colitis induced by dextran sulfate and ethanol].
Rev Peru Med Exp Salud Publica. 2017 Apr-Jun;34(2):176-182
OBJECTIVES.:
To investigate the effects of D-002, a mixture of 6 high molecular weight primary aliphatic alcohols, obtained from beeswax (Apis mellifera), on severe inflammatory ulcerative colitis (UC) induced by Dextran sulfate (DSS) and ethanol in rats (Ratus ratus).
MATERIALS AND METHODS.:
Rats were randomly distributed in six groups: a zero control to which no damage was caused, and five to which the UC was induced: a negative control (vehicle), three treated with D-002 (25, 100 and 400 mg/kg) and a positive control with sulfasalazine (200 mg/kg) (reference substance). Clinical manifestations (body weight variation, diarrhea and rectal bleeding), macroscopic and histological damage score, and myeloperoxidase (MPO) activity were quantified.
RESULTS.:
The oral treatment with D-002 (25, 100 and 400 mg/ kg) significantly prevented the decrease in body weight. The dose of 400 mg/kg reduced the presence of diarrhea and rectal bleeding, although its comparison with the negative control only reached statistical significance on diarrhea. D-002 (25, 100 and 400 mg/kg) significantly reduced the score of macroscopic lesions (40.0; 43.3 and 47.2% inhibition, respectively), the histological damage score (31.5; 53.7 and 67.1% inhibition, respectively) and the activity of MPO (73.2; 83.6 and 85.0% inhibition, respectively), compared to the negative control group. Sulfasalazine significantly reduced all variables studied.
CONCLUSIONS.:
D-002 (25, 100 and 400 mg/kg) significantly protected the colonic mucosa in rats with severe inflammatory UC induced by DSS and ethanol.
Rev Peru Med Exp Salud Publica. 2017 Apr-Jun;34(2):176-182
OBJECTIVES.:
To investigate the effects of D-002, a mixture of 6 high molecular weight primary aliphatic alcohols, obtained from beeswax (Apis mellifera), on severe inflammatory ulcerative colitis (UC) induced by Dextran sulfate (DSS) and ethanol in rats (Ratus ratus).
MATERIALS AND METHODS.:
Rats were randomly distributed in six groups: a zero control to which no damage was caused, and five to which the UC was induced: a negative control (vehicle), three treated with D-002 (25, 100 and 400 mg/kg) and a positive control with sulfasalazine (200 mg/kg) (reference substance). Clinical manifestations (body weight variation, diarrhea and rectal bleeding), macroscopic and histological damage score, and myeloperoxidase (MPO) activity were quantified.
RESULTS.:
The oral treatment with D-002 (25, 100 and 400 mg/ kg) significantly prevented the decrease in body weight. The dose of 400 mg/kg reduced the presence of diarrhea and rectal bleeding, although its comparison with the negative control only reached statistical significance on diarrhea. D-002 (25, 100 and 400 mg/kg) significantly reduced the score of macroscopic lesions (40.0; 43.3 and 47.2% inhibition, respectively), the histological damage score (31.5; 53.7 and 67.1% inhibition, respectively) and the activity of MPO (73.2; 83.6 and 85.0% inhibition, respectively), compared to the negative control group. Sulfasalazine significantly reduced all variables studied.
CONCLUSIONS.:
D-002 (25, 100 and 400 mg/kg) significantly protected the colonic mucosa in rats with severe inflammatory UC induced by DSS and ethanol.
Tuesday, November 28, 2017
Webinar November 29 on Apitherapy and Veterinary Medicine
CAA Webinar with French Veterinarian/Apitherapist Philippe Garcia
Date: November 29, 2017 Time: 2:30 pm (EST) Host: Canadian Apitherapy Association
GO TO: https://www.canadian-apitherapy-association.ca/webinars/caa-webinar-with-french-veterinarianapitherapist-philippe-garcia/
Our first webinar talking about the use of apitherapy in the realm of Veterinary Medicine.
Welcome and enjoy the conference of our French Veterinarian/Apitherapist Philippe Garcia.
The Webinar will be directly translated in English by Yann Loranger, Secretary/Treasurer of the Canadian Apitherapy Association.
Date: November 29, 2017 Time: 2:30 pm (EST) Host: Canadian Apitherapy Association
GO TO: https://www.canadian-apitherapy-association.ca/webinars/caa-webinar-with-french-veterinarianapitherapist-philippe-garcia/
Our first webinar talking about the use of apitherapy in the realm of Veterinary Medicine.
Welcome and enjoy the conference of our French Veterinarian/Apitherapist Philippe Garcia.
The Webinar will be directly translated in English by Yann Loranger, Secretary/Treasurer of the Canadian Apitherapy Association.
Monday, November 27, 2017
German Apitherapy Congress, Expo and Workshops March 23-27, 2018
Sunday, November 26, 2017
Saturday, November 25, 2017
Bee Venom Peptide May Help Treat Liver Cancer
The anti-hepatocellular carcinoma activity of Mel-P15 is mediated by natural killer cells
Oncol Lett. 2017 Dec;14(6):6901-6906
Mel-P15 is a peptide derived from melittin, the main toxic component in the venom of the European honeybee Apis mellifera.
In the present study, the antitumor effects of Mel-P15 and the underlying molecular mechanisms of these effects in vivo were investigated. Mel-P15 directly stimulated natural killer (NK) cell cytotoxicity in vitro, which was increased to 55.45% at a 4 µg/ml dose of Mel-P15.
In the mouse liver cancer (H22) xenograft mice model, Mel-P15 suppressed tumor growth in vivo; the tumor inhibitory rate was 61.15% following treatment with 2 mg/kg Mel-P15. In addition, the immune response was activated following Mel-P15 treatment. Mel-P15 treatment increased the spleen and thymus indices, promoted splenocyte proliferation, stimulated NK cytotoxicity and upregulated the secretion of cytokines, including interleukin-2, interferon-γ and tumor necrosis factor-α.
In addition, the tumor inhibitory effect of Mel-P15 on BEL-7402-bearing nude mice was abrogated by the selective depletion of NK cells via the intraperitoneal injection of an anti-asialo GM-1 antibody.
The results suggest that Mel-P15 inhibits tumor growth in vivo by promoting NK cell cytotoxicity. Mel-P15 may therefore be a potential immunotherapy candidate for the treatment of hepatocellular carcinoma.
Oncol Lett. 2017 Dec;14(6):6901-6906
Mel-P15 is a peptide derived from melittin, the main toxic component in the venom of the European honeybee Apis mellifera.
In the present study, the antitumor effects of Mel-P15 and the underlying molecular mechanisms of these effects in vivo were investigated. Mel-P15 directly stimulated natural killer (NK) cell cytotoxicity in vitro, which was increased to 55.45% at a 4 µg/ml dose of Mel-P15.
In the mouse liver cancer (H22) xenograft mice model, Mel-P15 suppressed tumor growth in vivo; the tumor inhibitory rate was 61.15% following treatment with 2 mg/kg Mel-P15. In addition, the immune response was activated following Mel-P15 treatment. Mel-P15 treatment increased the spleen and thymus indices, promoted splenocyte proliferation, stimulated NK cytotoxicity and upregulated the secretion of cytokines, including interleukin-2, interferon-γ and tumor necrosis factor-α.
In addition, the tumor inhibitory effect of Mel-P15 on BEL-7402-bearing nude mice was abrogated by the selective depletion of NK cells via the intraperitoneal injection of an anti-asialo GM-1 antibody.
The results suggest that Mel-P15 inhibits tumor growth in vivo by promoting NK cell cytotoxicity. Mel-P15 may therefore be a potential immunotherapy candidate for the treatment of hepatocellular carcinoma.
Thursday, November 23, 2017
Propolis, Honey Component Shows Anti-Inflammatory, Anti0cancer, and Anti-Viral Activities
Chrysin: Sources, beneficial pharmacological activities, and molecular mechanism of action
Phytochemistry. 2017 Nov 18;145:187-196
In recent years, public and scientific interest in plant flavonoids has tremendously increased because of their postulated health benefits.
This review was mainly focuses on the flavone chrysin (5,7-dihydroxyflavone), which occurs naturally in many plants, honey, and propolis. A number of in vitro and in vivo studies have revealed the therapeutic effects of chrysin against various diseases.
In general, chrysin exhibits many biological activities and pharmacological effects, including antioxidant, anti-inflammatory, anticancer, and antiviral activities. Moreover, many studies have reported on the bioavailability of chrysin. Because of its compromised bioavailability and enhanced protein stability, chrysin solid lipid nanoparticle (SLN) synthesis avoids proteolytic degradation and sustained release of drug delivery.
To clarify the mechanism of action of chrysin, researchers have investigated the structural binding relationship of chrysin through the docking computation method.
Phytochemistry. 2017 Nov 18;145:187-196
In recent years, public and scientific interest in plant flavonoids has tremendously increased because of their postulated health benefits.
This review was mainly focuses on the flavone chrysin (5,7-dihydroxyflavone), which occurs naturally in many plants, honey, and propolis. A number of in vitro and in vivo studies have revealed the therapeutic effects of chrysin against various diseases.
In general, chrysin exhibits many biological activities and pharmacological effects, including antioxidant, anti-inflammatory, anticancer, and antiviral activities. Moreover, many studies have reported on the bioavailability of chrysin. Because of its compromised bioavailability and enhanced protein stability, chrysin solid lipid nanoparticle (SLN) synthesis avoids proteolytic degradation and sustained release of drug delivery.
To clarify the mechanism of action of chrysin, researchers have investigated the structural binding relationship of chrysin through the docking computation method.
Wednesday, November 22, 2017
Ukrainian Honey-Propolis Gum Prevents Periodontal Disease
Made in Ukraine: periodontal disease treatment chewing gum
Ukraine Good News, 11/21/2017
Marta Tsap, an Ivano-Frankivsk National Medical University student, presented a chewing gum based on bactericidal and antiseptic properties of honey to prevent oral cavity diseases. The product is a great way to treat and prevent periodontal disease.
The chewing gum is based on caramelized honey, its effect was tested by the university dentistry faculty experts. The periodontium state of 6 out of 9 volunteers using the chewing gum for a month has improved.
In addition to honey, the gum contains wax, propolis and other beekeeping products.
As Marta says, the chewing gum is ready for serial production launch. The honey gum production start requires 209,000 UAH. The project will pay off in 2 years.
Tuesday, November 21, 2017
Propolis May Help Protect Against Radiation Damage
Radio-Modulatory Potential ofCaffeic Acid Phenethyl Ester: A Therapeutic Perspective
Anticancer Agents Med Chem. 2017 Nov 13
Use of natural agents is an upcoming area of research in cancer biology. Caffeic acid phenethyl ester has received great attention because of its therapeutic potential in various conditions including cancer. It is an active/abundant component of propolis. Propolis is a honey bee hive product produced by bees using their enzyme-rich digestive secretions on resinous mix, bee wax and pollen from plants. It is used to protect the beehive against bacteria and other infections.Although a lot of work has been done on chemotherapeutic aspects of CAPE, its role as a radiomodulator is yet to be delineated. It can act both as radioprotector and radiosensitizer. Depending on the tissue type it can modulate the radiation response by following different mechanisms.
This review will focus on the differential radiomodulatory effects of Caffeic Acid Phenethyl Ester in normal and cancer cells.Besides chemistry and bioavailability,it's potential as a therapeutic agent against radiation induced damage will also be discussed.
Anticancer Agents Med Chem. 2017 Nov 13
Use of natural agents is an upcoming area of research in cancer biology. Caffeic acid phenethyl ester has received great attention because of its therapeutic potential in various conditions including cancer. It is an active/abundant component of propolis. Propolis is a honey bee hive product produced by bees using their enzyme-rich digestive secretions on resinous mix, bee wax and pollen from plants. It is used to protect the beehive against bacteria and other infections.Although a lot of work has been done on chemotherapeutic aspects of CAPE, its role as a radiomodulator is yet to be delineated. It can act both as radioprotector and radiosensitizer. Depending on the tissue type it can modulate the radiation response by following different mechanisms.
This review will focus on the differential radiomodulatory effects of Caffeic Acid Phenethyl Ester in normal and cancer cells.Besides chemistry and bioavailability,it's potential as a therapeutic agent against radiation induced damage will also be discussed.
Monday, November 20, 2017
Propolis: This Bee Product is a Powerful Natural Immune Booster
By: Jordyn Cormier, November 19, 2017
Cold and flu season is in full swing, which means it’s time to start bringing on immune boosters again. Some of us will chug freshly squeezed orange juice. Others will slam shots of elderberry kombucha. A few will start force-feeding themselves and their family pungent raw garlic, all in a martyrish effort to avoid getting sick. But there is probably something even more powerful missing from your natural immune defense arsenal…propolis.
Propolis is one of the most undervalued natural immune boosters out there. Personally, I use my propolis spray daily. After years of getting sick every cold/flu season, a friend sent me some propolis to try. Ever since then, even the most inevitable colds seem to repel off of me. It’s pretty amazing.
If you are unfamiliar with propolis, it is a bee product, much like honey is—but that’s where the similarities end. Bees use propolis to keep their hives free of invaders and disease. It lines the hive walls and acts essentially as the immune system of the hive. And guess what—it can do the same for your body...
Cold and flu season is in full swing, which means it’s time to start bringing on immune boosters again. Some of us will chug freshly squeezed orange juice. Others will slam shots of elderberry kombucha. A few will start force-feeding themselves and their family pungent raw garlic, all in a martyrish effort to avoid getting sick. But there is probably something even more powerful missing from your natural immune defense arsenal…propolis.
Propolis is one of the most undervalued natural immune boosters out there. Personally, I use my propolis spray daily. After years of getting sick every cold/flu season, a friend sent me some propolis to try. Ever since then, even the most inevitable colds seem to repel off of me. It’s pretty amazing.
If you are unfamiliar with propolis, it is a bee product, much like honey is—but that’s where the similarities end. Bees use propolis to keep their hives free of invaders and disease. It lines the hive walls and acts essentially as the immune system of the hive. And guess what—it can do the same for your body...
Sunday, November 19, 2017
Turkish Honeys Beat Manuka Antibacterial Properties
Turkish honey beats world famous Manuka honey, study finds
DAILY SABAH
The flower honey from Turkey's southeastern province of Hakkari has much higher antimicrobial capabilities compared to the world famous Manuka honey of Australia and New Zealand, a new study has found.
Sevgi Kolaylı, a chemistry professor at the Karadeniz Technical University in Trabzon, says her recent study has found that several kinds of honey gathered from different parts of Turkey showed much higher antimicrobial activity compared to Manuka honey.
Kolaylı's study found that antimicrobial activities, which makes honey act against bacteria like antibiotics, are significantly higher in flower honey from Hakkari province compared to Manuka.
Several kinds of Turkish chestnut and oak honey also showed higher antibacterial properties, Kolaylı said.
The professor also said that Turkey ranks only behind China and Argentina as the world's top honey producing country.
"Antioxidant properties are richer in dark colored honey, whereas antimicrobial activities are higher in lighter colored honey," the professor explained, and added that Turkey is home to rich honey from both spectrums.
DAILY SABAH
The flower honey from Turkey's southeastern province of Hakkari has much higher antimicrobial capabilities compared to the world famous Manuka honey of Australia and New Zealand, a new study has found.
Sevgi Kolaylı, a chemistry professor at the Karadeniz Technical University in Trabzon, says her recent study has found that several kinds of honey gathered from different parts of Turkey showed much higher antimicrobial activity compared to Manuka honey.
Kolaylı's study found that antimicrobial activities, which makes honey act against bacteria like antibiotics, are significantly higher in flower honey from Hakkari province compared to Manuka.
Several kinds of Turkish chestnut and oak honey also showed higher antibacterial properties, Kolaylı said.
The professor also said that Turkey ranks only behind China and Argentina as the world's top honey producing country.
"Antioxidant properties are richer in dark colored honey, whereas antimicrobial activities are higher in lighter colored honey," the professor explained, and added that Turkey is home to rich honey from both spectrums.
Saturday, November 18, 2017
Ethanolic Extract of Polish Propolis Boosts Oral Hygiene
The Influence of Toothpaste Containing Australian Melaleuca alternifolia Oil and Ethanolic Extract of Polish Propolis on Oral Hygiene and Microbiome in Patients Requiring Conservative Procedures
Molecules. 2017 Nov 13;22(11). pii: E1957
The study was based on the use of a toothpaste with antiphlogistic activity, containing Australian Melaleuca alternifolia oil (tea tree oil-TTO) and ethanolic extract of Polish propolis (EEP). Fifty-one patients with varying conditions of the gingiva were divided into two groups.
The study group received the toothpaste with TTO and EEP, while the control group received the same toothpaste but without TTO and EEP. Approximal plaque index (API), simplified oral hygiene index (OHI-s) and modified sulcus bleeding index (mSBI) were assessed in three subsequent stages. During each examination, swabs were employed for microbiological inoculation. During the period of use of toothpastes with TTO and EEP, a significant reduction of the API was observed, as assessed upon the control visit after 7 days and after 28 days, compared to baseline. A statistically significant reduction of mSBI was observed after 7 and 28 days of using the toothpaste with TTO and EEP, as compared to the value upon the initial visit. Statistically significant differences in the OHI-s value were observed in the study group, which was using the active toothpaste. The use of a toothpaste containing TTO and EEP helps to maintain microbiome balance.
The observed stabilisation of bacterial microflora confirms the beneficial activity of toothpaste containing EEP and TTO compared to the control group, where the lack of these substances contributed to the emergence of qualitative and quantitative changes in oral microbiome.
Molecules. 2017 Nov 13;22(11). pii: E1957
The study was based on the use of a toothpaste with antiphlogistic activity, containing Australian Melaleuca alternifolia oil (tea tree oil-TTO) and ethanolic extract of Polish propolis (EEP). Fifty-one patients with varying conditions of the gingiva were divided into two groups.
The study group received the toothpaste with TTO and EEP, while the control group received the same toothpaste but without TTO and EEP. Approximal plaque index (API), simplified oral hygiene index (OHI-s) and modified sulcus bleeding index (mSBI) were assessed in three subsequent stages. During each examination, swabs were employed for microbiological inoculation. During the period of use of toothpastes with TTO and EEP, a significant reduction of the API was observed, as assessed upon the control visit after 7 days and after 28 days, compared to baseline. A statistically significant reduction of mSBI was observed after 7 and 28 days of using the toothpaste with TTO and EEP, as compared to the value upon the initial visit. Statistically significant differences in the OHI-s value were observed in the study group, which was using the active toothpaste. The use of a toothpaste containing TTO and EEP helps to maintain microbiome balance.
The observed stabilisation of bacterial microflora confirms the beneficial activity of toothpaste containing EEP and TTO compared to the control group, where the lack of these substances contributed to the emergence of qualitative and quantitative changes in oral microbiome.
Honey: A Therapeutic Agent for Disorders of the Skin
Cent Asian J Glob Health. 5(1):241
Problems with conventional treatments for a range of dermatological disorders have led scientists to search for new compounds of therapeutic value. Efforts have included the evaluation of natural products such as honey. Manuka honey, for example, has been scientifically recognised for its anti-microbial and wound healing properties and is now used clinically as a topical treatment for wound infections. In this review, scientific evidence for the effectiveness of honey in the treatment of wounds and other skin conditions is evaluated. A plethora of in vitro studies have revealed that honeys from all over the world have potent antimicrobial activity against skin relevant microbes. Moreover, a number of in vitro studies suggest that honey is able to modulate the skin immune system.
Clinical research has shown honey to be efficacious in promoting the healing of partial thickness burn wounds while its effectiveness in the treatment of non-burn acute wounds and chronic wounds is conflicted. Published research investigating the efficacy of honey in the treatment of other types of skin disorders is limited. Nevertheless, positive effects have been reported, for example, kanuka honey from New Zealand was shown to have therapeutic value in the treatment of rosacea. Anti-carcinogenic effects of honey have also been observed in vitro and in a murine model of melanoma.
It can be concluded that honey is a biologically active and clinically interesting substance but more research is necessary for a comprehensive understanding of its medicinal value in dermatology.
Problems with conventional treatments for a range of dermatological disorders have led scientists to search for new compounds of therapeutic value. Efforts have included the evaluation of natural products such as honey. Manuka honey, for example, has been scientifically recognised for its anti-microbial and wound healing properties and is now used clinically as a topical treatment for wound infections. In this review, scientific evidence for the effectiveness of honey in the treatment of wounds and other skin conditions is evaluated. A plethora of in vitro studies have revealed that honeys from all over the world have potent antimicrobial activity against skin relevant microbes. Moreover, a number of in vitro studies suggest that honey is able to modulate the skin immune system.
Clinical research has shown honey to be efficacious in promoting the healing of partial thickness burn wounds while its effectiveness in the treatment of non-burn acute wounds and chronic wounds is conflicted. Published research investigating the efficacy of honey in the treatment of other types of skin disorders is limited. Nevertheless, positive effects have been reported, for example, kanuka honey from New Zealand was shown to have therapeutic value in the treatment of rosacea. Anti-carcinogenic effects of honey have also been observed in vitro and in a murine model of melanoma.
It can be concluded that honey is a biologically active and clinically interesting substance but more research is necessary for a comprehensive understanding of its medicinal value in dermatology.
Friday, November 17, 2017
Powder Containing Extract of Green and Red Propolis a Possible Antimicrobial Agent
Evaluation of the antimicrobial activity and cytotoxic effect of hydroxyapatite containing Brazilian propolis
Biomed Mater. 2017 Nov 14
The aim of this work was to produce hydroxyapatite powder (HA) containing dry extract of green and red propolis, and to evaluate possible bactericidal activity of the materials in a short period of time through a fast release system.
The ethanolic extracts of green and red propolis (EEP) were incorporated into the material by spray drying. After release tests, powders containing dry EEP were characterized regarding the content of total phenolics and flavonoids. Materials characterization was made by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The antimicrobial activity was evaluated by plate colony counting, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against Staphylococcus aureus (S. aureus). The cytotoxicity of the materials was determined by the neutral red incorporation method. Materials showed apparently spherical morphology, indicating a decrease in the agglomeration degree with the propolis addition.
HA and propolis characteristic functional groups were observed in FTIR. Materials had higher release of phenolics and lesser amounts of flavonoids when compared to the EEP, with the higher amounts of flavonoids observed for HA with red propolis. The bactericidal effect for all materials was observed within the interval of 0.5 to 1 hour. All materials showed lesser inhibitory activity (MIC) and higher bactericidal activity (MBC) when compared to the EEP, with the best results attributed to HA with red propolis. The values of IC50 (concentration needed to inhibit 50% of the cell growth) obtained from the cytotoxicity assay for HA with green and red propolis lied between MIC and MCB.
Considering these results, it is suggested that HA with propolis may be used as a possible antimicrobial agent, inhibiting the growth of S. aureus, despite further in vivo biocompatibility should be investigated before using this material as a medical device with bactericidal potential.
Biomed Mater. 2017 Nov 14
The aim of this work was to produce hydroxyapatite powder (HA) containing dry extract of green and red propolis, and to evaluate possible bactericidal activity of the materials in a short period of time through a fast release system.
The ethanolic extracts of green and red propolis (EEP) were incorporated into the material by spray drying. After release tests, powders containing dry EEP were characterized regarding the content of total phenolics and flavonoids. Materials characterization was made by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The antimicrobial activity was evaluated by plate colony counting, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against Staphylococcus aureus (S. aureus). The cytotoxicity of the materials was determined by the neutral red incorporation method. Materials showed apparently spherical morphology, indicating a decrease in the agglomeration degree with the propolis addition.
HA and propolis characteristic functional groups were observed in FTIR. Materials had higher release of phenolics and lesser amounts of flavonoids when compared to the EEP, with the higher amounts of flavonoids observed for HA with red propolis. The bactericidal effect for all materials was observed within the interval of 0.5 to 1 hour. All materials showed lesser inhibitory activity (MIC) and higher bactericidal activity (MBC) when compared to the EEP, with the best results attributed to HA with red propolis. The values of IC50 (concentration needed to inhibit 50% of the cell growth) obtained from the cytotoxicity assay for HA with green and red propolis lied between MIC and MCB.
Considering these results, it is suggested that HA with propolis may be used as a possible antimicrobial agent, inhibiting the growth of S. aureus, despite further in vivo biocompatibility should be investigated before using this material as a medical device with bactericidal potential.
Thursday, November 16, 2017
Ginger and Propolis Exert Neuroprotective Effects
Ginger and Propolis Exert Neuroprotective Effects against Monosodium Glutamate-Induced Neurotoxicity in Rats
Molecules. 2017 Nov 8;22(11). pii: E1928
Central nervous system cytotoxicity is linked to neurodegenerative disorders. The objective of the study was to investigate whether monosodium glutamate (MSG) neurotoxicity can be reversed by natural products, such as ginger or propolis, in male rats.
Four different groups of Wistar rats were utilized in the study. Group A served as a normal control, whereas group B was orally administered with MSG (100 mg/kg body weight, via oral gavage). Two additional groups, C and D, were given MSG as group B along with oral dose (500 mg/kg body weight) of either ginger or propolis (600 mg/kg body weight) once a day for two months. At the end, the rats were sacrificed, and the brain tissue was excised and levels of neurotransmitters, ß-amyloid, and DNA oxidative marker 8-OHdG were estimated in the brain homogenates. Further, formalin-fixed and paraffin-embedded brain sections were used for histopathological evaluation.
The results showed that MSG increased lipid peroxidation, nitric oxide, neurotransmitters, and 8-OHdG as well as registered an accumulation of ß-amyloid peptides compared to normal control rats. Moreover, significant depletions of glutathione, superoxide dismutase, and catalase as well as histopathological alterations in the brain tissue of MSG-treated rats were noticed in comparison with the normal control. In contrast, treatment with ginger greatly attenuated the neurotoxic effects of MSG through suppression of 8-OHdG and β-amyloid accumulation as well as alteration of neurotransmitter levels.
Further improvements were also noticed based on histological alterations and reduction of neurodegeneration in the brain tissue. A modest inhibition of the neurodegenerative markers was observed by propolis.
The study clearly indicates a neuroprotective effect of ginger and propolis against MSG-induced neurodegenerative disorders and these beneficial effects could be attributed to the polyphenolic compounds present in these natural products.
Molecules. 2017 Nov 8;22(11). pii: E1928
Central nervous system cytotoxicity is linked to neurodegenerative disorders. The objective of the study was to investigate whether monosodium glutamate (MSG) neurotoxicity can be reversed by natural products, such as ginger or propolis, in male rats.
Four different groups of Wistar rats were utilized in the study. Group A served as a normal control, whereas group B was orally administered with MSG (100 mg/kg body weight, via oral gavage). Two additional groups, C and D, were given MSG as group B along with oral dose (500 mg/kg body weight) of either ginger or propolis (600 mg/kg body weight) once a day for two months. At the end, the rats were sacrificed, and the brain tissue was excised and levels of neurotransmitters, ß-amyloid, and DNA oxidative marker 8-OHdG were estimated in the brain homogenates. Further, formalin-fixed and paraffin-embedded brain sections were used for histopathological evaluation.
The results showed that MSG increased lipid peroxidation, nitric oxide, neurotransmitters, and 8-OHdG as well as registered an accumulation of ß-amyloid peptides compared to normal control rats. Moreover, significant depletions of glutathione, superoxide dismutase, and catalase as well as histopathological alterations in the brain tissue of MSG-treated rats were noticed in comparison with the normal control. In contrast, treatment with ginger greatly attenuated the neurotoxic effects of MSG through suppression of 8-OHdG and β-amyloid accumulation as well as alteration of neurotransmitter levels.
Further improvements were also noticed based on histological alterations and reduction of neurodegeneration in the brain tissue. A modest inhibition of the neurodegenerative markers was observed by propolis.
The study clearly indicates a neuroprotective effect of ginger and propolis against MSG-induced neurodegenerative disorders and these beneficial effects could be attributed to the polyphenolic compounds present in these natural products.
Wednesday, November 15, 2017
Antimicrobial Effect of Ginger, Garlic, Honey, and Lemon Extracts on Streptococcus mutans
J Contemp Dent Pract. 2017 Nov 1;18(11):1004-1008.
The aim of this study was to evaluate the effect of lemon, ginger, garlic, and honey extracts on Streptococcus mutans.
MATERIALS AND METHODS:
Commercially obtained honey, ginger, garlic, and lemon were included in the study to evaluate its efficacy in isolation and in combination against S. mutans. The efficacies of extracts were tested using well diffusion method, and its effect was evaluated by measuring the zone of inhibition around the well. Antimicrobial activity of the extracts was carried out individually and compared considering triplicates of all the extracts.
RESULTS:
When individual comparison of the extracts was made, garlic showed greatest antimicrobial activity with a mean zone of inhibition (34.9 ± 0.58 mm) and honey showed least antimicrobial activity (0.5 ± 0.6 mm). When combinations of extracts were tested against S. mutans, lemon and garlic combination showed the greatest zone of inhibition (27.6 ± 0.43 mm) compared with other combinations, and ginger + lemon combinations showed the least zone of inhibition (12.6 ± 0.43 mm).
CONCLUSION:
This study concluded that garlic showed a greatest antimicrobial effect against S. mutans when compared with other preparations individually and garlic and lemon showed greatest zone of inhibition in combination than other preparations.
CLINICAL SIGNIFICANCE:
Antibiotics and other chemical agents are mainly used to treat the common dental infections. However, due to the excessive use, it can result in antibiotic resistance. Hence, herbal medicines with medicinal values should be replaced with conventional methods.
The aim of this study was to evaluate the effect of lemon, ginger, garlic, and honey extracts on Streptococcus mutans.
MATERIALS AND METHODS:
Commercially obtained honey, ginger, garlic, and lemon were included in the study to evaluate its efficacy in isolation and in combination against S. mutans. The efficacies of extracts were tested using well diffusion method, and its effect was evaluated by measuring the zone of inhibition around the well. Antimicrobial activity of the extracts was carried out individually and compared considering triplicates of all the extracts.
RESULTS:
When individual comparison of the extracts was made, garlic showed greatest antimicrobial activity with a mean zone of inhibition (34.9 ± 0.58 mm) and honey showed least antimicrobial activity (0.5 ± 0.6 mm). When combinations of extracts were tested against S. mutans, lemon and garlic combination showed the greatest zone of inhibition (27.6 ± 0.43 mm) compared with other combinations, and ginger + lemon combinations showed the least zone of inhibition (12.6 ± 0.43 mm).
CONCLUSION:
This study concluded that garlic showed a greatest antimicrobial effect against S. mutans when compared with other preparations individually and garlic and lemon showed greatest zone of inhibition in combination than other preparations.
CLINICAL SIGNIFICANCE:
Antibiotics and other chemical agents are mainly used to treat the common dental infections. However, due to the excessive use, it can result in antibiotic resistance. Hence, herbal medicines with medicinal values should be replaced with conventional methods.
Tuesday, November 14, 2017
Honey Polyphenols May Help Treat Cardiovascular Diseases, Diabetes, Cancer, and Neurodegenerative Disorders
Beneficial roles of honey polyphenols against some human degenerative diseases: A review
Pharmacol Rep. 2017 Jul 4;69(6):1194-1205
Honey contains many active constituents and antioxidants such as polyphenols. Polyphenols are phytochemicals, a generic term for the several thousand plant-based molecules with antioxidant properties.
Many in vitro studies in human cell cultures as well as many animal studies confirm the protective effect of polyphenols on a number of diseases such as cardiovascular diseases (CVD), diabetes, cancer, neurodegenerative diseases, pulmonary diseases, liver diseases and so on. Nevertheless, it is challenging to identify the specific biological mechanism underlying individual polyphenols and to determine how polyphenols impact human health. To date, several studies have attempted to elucidate the molecular pathway for specific polyphenols acting against particular diseases.
In this review, we report on the various polyphenols present in different types of honey according to their classification, source, and specific functions and discuss several of the honey polyphenols with the most therapeutic potential to exert an effect on the various pathologies of some major diseases including CVD, diabetes, cancer, and neurodegenerative diseases.
Pharmacol Rep. 2017 Jul 4;69(6):1194-1205
Honey contains many active constituents and antioxidants such as polyphenols. Polyphenols are phytochemicals, a generic term for the several thousand plant-based molecules with antioxidant properties.
Many in vitro studies in human cell cultures as well as many animal studies confirm the protective effect of polyphenols on a number of diseases such as cardiovascular diseases (CVD), diabetes, cancer, neurodegenerative diseases, pulmonary diseases, liver diseases and so on. Nevertheless, it is challenging to identify the specific biological mechanism underlying individual polyphenols and to determine how polyphenols impact human health. To date, several studies have attempted to elucidate the molecular pathway for specific polyphenols acting against particular diseases.
In this review, we report on the various polyphenols present in different types of honey according to their classification, source, and specific functions and discuss several of the honey polyphenols with the most therapeutic potential to exert an effect on the various pathologies of some major diseases including CVD, diabetes, cancer, and neurodegenerative diseases.
Monday, November 13, 2017
Royal Jelly as Skin Moisturizing Agent
10-Hydroxy-2-Decenoic Acid in Royal Jelly Extract Induced Both Filaggrin and Amino Acid in a Cultured Human Three-Dimensional Epidermis Model
Cosmetics 2017, 4(4), 48
Royal jelly (RJ) is a natural product which the honeybee secretes as a special diet for a queen bee. It is one of the natural products in which various functionalities, such as antibacterial effects, immunomodulating properties, and estrogen-like action, were reported.
We investigated the effect of the RJ extract on the moisturizing effect by topical application in humans. The stratum corneum moisture was increased significantly after four weeks by using the RJ extract lotion compared to placebo lotion. RJ extract contained a characteristic ingredient, 10-hydroxy-2-decenoic acid (10H2DA) and 10-hydroxydecanoic acid (10HDAA), etc. However, the mechanism of stratum corneum moisture and its contributing ingredient have not yet been elucidated.
We have investigated the effects of 10H2DA and 10HDAA on the free amino acids content in the stratum corneum using a cultured human three-dimensional epidermis model. Additionally, the effect of 10H2DA and 10HDAA on the amounts of filaggrin (FLG) and aquaporin 3 (AQP3) were investigated at the mRNA level and by immunohistochemistry using a cultured human epidermis model. It was determined that 10H2DA increases the free amino acids in the stratum corneum of the cultured human epidermis model, and that it increased FLG on both the mRNA and protein levels. On the other hand, these actions are not observed by treatment of 10HDAA. The mRNA and protein level of AQP3 did not increase with 10H2DA or 10HDAA use.
It was thought that the increase in the amount of FLG and the increase in the free amino acids of the epidermis and the stratum corneum, respectively, by 10H2DA were participating in the moisturizing function of the stratum corneum by the continuous use of RJ extract lotion.
Cosmetics 2017, 4(4), 48
Royal jelly (RJ) is a natural product which the honeybee secretes as a special diet for a queen bee. It is one of the natural products in which various functionalities, such as antibacterial effects, immunomodulating properties, and estrogen-like action, were reported.
We investigated the effect of the RJ extract on the moisturizing effect by topical application in humans. The stratum corneum moisture was increased significantly after four weeks by using the RJ extract lotion compared to placebo lotion. RJ extract contained a characteristic ingredient, 10-hydroxy-2-decenoic acid (10H2DA) and 10-hydroxydecanoic acid (10HDAA), etc. However, the mechanism of stratum corneum moisture and its contributing ingredient have not yet been elucidated.
We have investigated the effects of 10H2DA and 10HDAA on the free amino acids content in the stratum corneum using a cultured human three-dimensional epidermis model. Additionally, the effect of 10H2DA and 10HDAA on the amounts of filaggrin (FLG) and aquaporin 3 (AQP3) were investigated at the mRNA level and by immunohistochemistry using a cultured human epidermis model. It was determined that 10H2DA increases the free amino acids in the stratum corneum of the cultured human epidermis model, and that it increased FLG on both the mRNA and protein levels. On the other hand, these actions are not observed by treatment of 10HDAA. The mRNA and protein level of AQP3 did not increase with 10H2DA or 10HDAA use.
It was thought that the increase in the amount of FLG and the increase in the free amino acids of the epidermis and the stratum corneum, respectively, by 10H2DA were participating in the moisturizing function of the stratum corneum by the continuous use of RJ extract lotion.
Sunday, November 12, 2017
New Product Uses Propolis, Bee Venom, Wax Moth Extract to Treat Joint and Tissue Pain
SustaFix LLC Created a Honey-Based Product for Treating Joint and Tissue Pain
November 10, 2017 --(PR.com)-- A novel honey-based product for treating joint and tisssue pain has been created and manufactured in Atlanta, Georgia. SustaFix gel contains propolis extract, bee venom, bee extract, beeswax, cedar sap, olive oil, horse chestnut extract and wax moth extract. Additional ingredients embaded in product's formula include vitamins C, B1, and B5, which play role in strengthening the vein muscle walls and tissue regeneration. Other ingredients include substances that possess vasodilatory and painkilling properties. SustaFix's action is pointed at relieving the inflammation, eliminating pain and swelling of joints, and repressing the symptoms of arthritis and arthrosis.
The use of honey for medical purposes is known for decades as alternative or complementary medicine. Today it is gaining popularity not only in the United States, but Europe and Asia as well. According to a research, made by National Center for Complementary and Alternative Medicine in 2007 (https://nccih.nih.gov/sites/nccam.nih.gov/files/camuse.pdf), almost 40 percent of adults in America use some form of alternative medicine. It is known that honey is widely used in treating a variety of medical conditions, including arthritis, joint a, promotes burn and wound healing, and helps in suppressing cough.
SustaFix beeswax gel smooth consistency makes it easily applicable on the affected areas. A small amount of the gel should be spread on the area of concern and then - gently massaged until its full absorption. Tube contains 100 ml substance, that should be applied once or twice a day depending on the severity of the condition.
November 10, 2017 --(PR.com)-- A novel honey-based product for treating joint and tisssue pain has been created and manufactured in Atlanta, Georgia. SustaFix gel contains propolis extract, bee venom, bee extract, beeswax, cedar sap, olive oil, horse chestnut extract and wax moth extract. Additional ingredients embaded in product's formula include vitamins C, B1, and B5, which play role in strengthening the vein muscle walls and tissue regeneration. Other ingredients include substances that possess vasodilatory and painkilling properties. SustaFix's action is pointed at relieving the inflammation, eliminating pain and swelling of joints, and repressing the symptoms of arthritis and arthrosis.
The use of honey for medical purposes is known for decades as alternative or complementary medicine. Today it is gaining popularity not only in the United States, but Europe and Asia as well. According to a research, made by National Center for Complementary and Alternative Medicine in 2007 (https://nccih.nih.gov/sites/nccam.nih.gov/files/camuse.pdf), almost 40 percent of adults in America use some form of alternative medicine. It is known that honey is widely used in treating a variety of medical conditions, including arthritis, joint a, promotes burn and wound healing, and helps in suppressing cough.
SustaFix beeswax gel smooth consistency makes it easily applicable on the affected areas. A small amount of the gel should be spread on the area of concern and then - gently massaged until its full absorption. Tube contains 100 ml substance, that should be applied once or twice a day depending on the severity of the condition.
Saturday, November 11, 2017
Propolis Prevents Biodegradation of Natural Rubber
Propolis as an antidegradant and biocidal agent for natural rubber
Journal of Applied Polymer Science
First published: 7 November 2017
The numerous applications of polymers in many areas of our lives make it necessary to protect them from aging. Increases in the safety and time of polymer material exploitation demands the application of antidegradants with broad scopes of action.
In this study, crude propolis, originating from two geographical regions of Poland, was used to protect peroxide-cured natural-rubber vulcanizates filled with silica or carbon black from degradation by oxygen, ozone, and microorganisms. Thermogravimetry analysis confirmed the stability of propolis under the curing conditions. From vulcametric torque measurements and determinations of the mechanical properties, we determined that the incorporation of propolis did not adversely affect the performance of the natural-rubber vulcanizates.
The addition of propolis to the rubber mix made the vulcanizates resistant to thermooxidative and ozone aging and protected them from biodegradation in soil and the action of microorganisms.
Journal of Applied Polymer Science
First published: 7 November 2017
The numerous applications of polymers in many areas of our lives make it necessary to protect them from aging. Increases in the safety and time of polymer material exploitation demands the application of antidegradants with broad scopes of action.
In this study, crude propolis, originating from two geographical regions of Poland, was used to protect peroxide-cured natural-rubber vulcanizates filled with silica or carbon black from degradation by oxygen, ozone, and microorganisms. Thermogravimetry analysis confirmed the stability of propolis under the curing conditions. From vulcametric torque measurements and determinations of the mechanical properties, we determined that the incorporation of propolis did not adversely affect the performance of the natural-rubber vulcanizates.
The addition of propolis to the rubber mix made the vulcanizates resistant to thermooxidative and ozone aging and protected them from biodegradation in soil and the action of microorganisms.
Friday, November 10, 2017
Bee Venom Acupuncture Helps Treat Lower Back Pain
Efficacy of Bee Venom Acupuncture for Chronic Low Back Pain: A Randomized, Double-Blinded, Sham-Controlled Trial
Toxins 2017, 9(11), 361
Bee venom acupuncture (BVA) is an effective treatment for chronic low back pain (CLBP) through the pharmacological effects of bee venom and the simultaneous stimulation of acupoints. However, evidence of its efficacy and safety in humans remains unclear.
Using a double-blind, randomized study, 54 patients with non-specific CLBP were assigned to the BVA and sham groups. All participants underwent six sessions of real or sham BVA for 3 weeks, in addition to administration of 180 mg of loxonin per day. The primary outcome, that is, “bothersomeness” derived from back pain, was assessed using the visual analog scale. Secondary outcomes included pain intensity, dysfunction related to back pain (Oswestry Disability Index), quality of life (EuroQol 5-Dimension), and depressive mood (Beck’s depression inventory). Outcomes were evaluated every week during the treatment period and followed up at weeks 4, 8, and 12.
After 3 weeks of the treatment, significant improvements were observed in the bothersomeness, pain intensity, and functional status in the BVA group compared with the sham group. Although minimal adverse events were observed in both groups, subsequent recovery was achieved without treatment.
Consequently, our results suggest that it can be used along with conventional pharmacological therapies for the treatment of CLBP.
Toxins 2017, 9(11), 361
Bee venom acupuncture (BVA) is an effective treatment for chronic low back pain (CLBP) through the pharmacological effects of bee venom and the simultaneous stimulation of acupoints. However, evidence of its efficacy and safety in humans remains unclear.
Using a double-blind, randomized study, 54 patients with non-specific CLBP were assigned to the BVA and sham groups. All participants underwent six sessions of real or sham BVA for 3 weeks, in addition to administration of 180 mg of loxonin per day. The primary outcome, that is, “bothersomeness” derived from back pain, was assessed using the visual analog scale. Secondary outcomes included pain intensity, dysfunction related to back pain (Oswestry Disability Index), quality of life (EuroQol 5-Dimension), and depressive mood (Beck’s depression inventory). Outcomes were evaluated every week during the treatment period and followed up at weeks 4, 8, and 12.
After 3 weeks of the treatment, significant improvements were observed in the bothersomeness, pain intensity, and functional status in the BVA group compared with the sham group. Although minimal adverse events were observed in both groups, subsequent recovery was achieved without treatment.
Consequently, our results suggest that it can be used along with conventional pharmacological therapies for the treatment of CLBP.
Thursday, November 09, 2017
Charles Mraz Apitherapy Course and Conference (CMACC)
Theory In Practice, A Hands-On Approach
November 10-12 2017
The Redondo Beach Hotel
400 N. Harbor Drive
Redondo Beach, CA 90277
310-376-0746
November 10-12 2017
The Redondo Beach Hotel
400 N. Harbor Drive
Redondo Beach, CA 90277
310-376-0746
Bee Venom Component May Help Treat Hepatitis B
Medical Express, 11/7/2017
"We now have a drug that can knock down hepatitis B surface antigen and determine whether or not we can actually cure people with that," Dr. Lanford said.
The drug is delivered by subcutaneous (under the skin) injection. Scientists designed a molecule that delivers the medicine directly to the liver where it binds to a receptor. Then, another molecule that's derived from bee venom, helps break through membranes in the liver cells to deliver the medicine directly into the cytoplasm of the cells where it takes effect. The siRNA interferes with the expression of the HBV messenger RNA that produces the surface antigen...
"We now have a drug that can knock down hepatitis B surface antigen and determine whether or not we can actually cure people with that," Dr. Lanford said.
The drug is delivered by subcutaneous (under the skin) injection. Scientists designed a molecule that delivers the medicine directly to the liver where it binds to a receptor. Then, another molecule that's derived from bee venom, helps break through membranes in the liver cells to deliver the medicine directly into the cytoplasm of the cells where it takes effect. The siRNA interferes with the expression of the HBV messenger RNA that produces the surface antigen...
Wednesday, November 08, 2017
Indian Propolis as a Potential Nutraceutical Candidate
Marker-based standardization and investigation of nutraceutical potential of Indian propolis
J Integr Med. 2017 Nov;15(6):483-494
OBJECTIVE:
Propolis, a resinous material collected by honey bees from various plants, has been explored globally for its medicinal and nutritional properties. However, research over Indian propolis is at infancy. This study was designed to investigate nutraceutical potential of Indian propolis.
METHODS:
In the present study, propolis extract was standardized with respect to markers caffeic acid phenethyl ester, caffeic acid, galangin, luteolin, curcumin, apigenin, pinocembrin and quercetin by new high-performance thin-layer chromatographic (HPTLC) methods. The physico-chemical analysis, residues analysis and in vitro antioxidant activity analysis were performed. Nutraceutical value was examined in terms of fats, fibers, minerals, proteins, polysaccharides, total carbohydrates, and energy value.
RESULTS:
The developed HPTLC methods were found to be simple, reliable accurate, and the validation parameters were within the limits of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines. Macerated ethanolic extract of propolis (MEEP) was found to have polyphenolic content of (20.99 ± 0.24) mg/g and flavonoids content of (8.39 ± 0.04) mg/g. MEEP was found to comprise of (283.33 ± 51.31) g/kg fats, (30.07 ± 7.30) g/kg fibers, (102.56 ± 2.84) g/kg proteins and (389.36 ± 57.50) g/kg carbohydrate with a calorie value of (38 409.33 ± 6 169.80) kJ/kg. It was found that Indian propolis exhibited high nutraceutical value and showed absence of pesticides and heavy metals. The MEEP showed in vitro antioxidant activity with inhibitory concentration of (12.24 ± 4.64) μg/mL.
CONCLUSION:
The present work explores Indian propolis as a potential nutritious candidate. The proposed analytical methods can be applied in future screening of the quality of Indian propolis.
J Integr Med. 2017 Nov;15(6):483-494
OBJECTIVE:
Propolis, a resinous material collected by honey bees from various plants, has been explored globally for its medicinal and nutritional properties. However, research over Indian propolis is at infancy. This study was designed to investigate nutraceutical potential of Indian propolis.
METHODS:
In the present study, propolis extract was standardized with respect to markers caffeic acid phenethyl ester, caffeic acid, galangin, luteolin, curcumin, apigenin, pinocembrin and quercetin by new high-performance thin-layer chromatographic (HPTLC) methods. The physico-chemical analysis, residues analysis and in vitro antioxidant activity analysis were performed. Nutraceutical value was examined in terms of fats, fibers, minerals, proteins, polysaccharides, total carbohydrates, and energy value.
RESULTS:
The developed HPTLC methods were found to be simple, reliable accurate, and the validation parameters were within the limits of the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use guidelines. Macerated ethanolic extract of propolis (MEEP) was found to have polyphenolic content of (20.99 ± 0.24) mg/g and flavonoids content of (8.39 ± 0.04) mg/g. MEEP was found to comprise of (283.33 ± 51.31) g/kg fats, (30.07 ± 7.30) g/kg fibers, (102.56 ± 2.84) g/kg proteins and (389.36 ± 57.50) g/kg carbohydrate with a calorie value of (38 409.33 ± 6 169.80) kJ/kg. It was found that Indian propolis exhibited high nutraceutical value and showed absence of pesticides and heavy metals. The MEEP showed in vitro antioxidant activity with inhibitory concentration of (12.24 ± 4.64) μg/mL.
CONCLUSION:
The present work explores Indian propolis as a potential nutritious candidate. The proposed analytical methods can be applied in future screening of the quality of Indian propolis.
Tuesday, November 07, 2017
Honey May Help Eradicate Methicillin Sesistant Staphylococcus Aureus (MRSA)
Randomised controlled trial of honey versus mupirocin to decolonise patients with nasal colonisation of methicillin resistant Staphylococcus aureus and the lessons learned
J Hosp Infect. 2017 Oct 26. pii: S0195-6701(17)30582-0
AIM:
To describe the learning experience from a randomised controlled trial (RCT) comparing the efficacy of medical-grade honey (MGH) with mupirocin 2% for the eradication of nasal methicillin-resistant Staphylococcus aureus (MRSA).
METHODS:
Patients colonised in the nose with MRSA and age ≥18 years were recruited. Participants received either one or two courses of MGH or mupirocin 2%, three times per day for five consecutive days.
RESULTS:
The proportion of patients who were decolonised after one or two courses of treatment was not significantly different between MGH (18/42; 42.8%; 95% CI: 27.7% - 59.0%), and mupirocin 2% (25/44; 56.8%; 95% CI: 41.0% - 71.7%). Non-nasal MRSA colonisation was significantly associated with persistent nasal colonisation, (p = 0.003, OR, 5.186, 95% CI: 1.736 - 15.489). The rate of new acquisition of mupirocin resistance was 9.75%.
CONCLUSIONS:
Although not significant, a decolonisation rate of 42.8% for MGH was impressive. In the absence of other effective agents, MGH has a potentially clinically important role. Our findings suggest this strategy, which has the potential to combat antimicrobial resistance, should be assessed in similar but larger studies. Studies of natural agents where there is no commercial patent and which are, therefore, unlikely to be funded by industry, need to be adequately funded by national and or international agencies so that well designed multi-centre RCTs can be carried out which may provide alternatives to antibiotics where resistance is a continuing challenge. The lessons learned may help guide future studies in determining realistic recruitment potential.
J Hosp Infect. 2017 Oct 26. pii: S0195-6701(17)30582-0
AIM:
To describe the learning experience from a randomised controlled trial (RCT) comparing the efficacy of medical-grade honey (MGH) with mupirocin 2% for the eradication of nasal methicillin-resistant Staphylococcus aureus (MRSA).
METHODS:
Patients colonised in the nose with MRSA and age ≥18 years were recruited. Participants received either one or two courses of MGH or mupirocin 2%, three times per day for five consecutive days.
RESULTS:
The proportion of patients who were decolonised after one or two courses of treatment was not significantly different between MGH (18/42; 42.8%; 95% CI: 27.7% - 59.0%), and mupirocin 2% (25/44; 56.8%; 95% CI: 41.0% - 71.7%). Non-nasal MRSA colonisation was significantly associated with persistent nasal colonisation, (p = 0.003, OR, 5.186, 95% CI: 1.736 - 15.489). The rate of new acquisition of mupirocin resistance was 9.75%.
CONCLUSIONS:
Although not significant, a decolonisation rate of 42.8% for MGH was impressive. In the absence of other effective agents, MGH has a potentially clinically important role. Our findings suggest this strategy, which has the potential to combat antimicrobial resistance, should be assessed in similar but larger studies. Studies of natural agents where there is no commercial patent and which are, therefore, unlikely to be funded by industry, need to be adequately funded by national and or international agencies so that well designed multi-centre RCTs can be carried out which may provide alternatives to antibiotics where resistance is a continuing challenge. The lessons learned may help guide future studies in determining realistic recruitment potential.
Monday, November 06, 2017
Malaysian Stingless Bee Honey High in Anti-Oxidants
Malaysian stingless bee and Tualang honeys: A comparative characterization of total antioxidant capacity and phenolic profile using liquid chromatography-mass spectrometry
LWT - Food Science and Technology
Volume 89, March 2018, Pages 1-9
• Malaysian Stingless bee honey has higher vitamin C, protein, polyphenols content than Tualang honey.
• LC-MS identified several phenolic compounds in stingless bee honey.
• Stingless bee honey had the highest anti-oxidant performance.
• Polyphenol content of stingless bee honey was associated with antioxidant activities.
This study aims to examine and compare the phenolic profile and antioxidant properties of two kinds of honey: Stingless bee honey (Kelulut honey) and Tualang honeys. Using liquid chromatography mass spectrometry, a total of eighteen phenolic acids and flavonoids have been identified in Kelulut honey samples. The phenolic content and total antioxidant capacity (three assays: DPPH, ABTS, and ORAC) for Kelulut and Tualang honeys were evaluated and statistically estimated. There was a significant (p < 0.05) phenolic content (228.09 ± 7.9–235.28 ± 0.6 mg gallic acid equivalent per kg) and flavonoid content (97.88 ± 10.1–101.5 ± 11.4 mg catechin equivalent per kg) in Kelulut honey samples. The Kelulut honey samples have demonstrated a significantly stronger antioxidant capacity than Tualang honey samples. The correlations between antioxidant results and polyphenols content were found to be statistically significant (P < 0.05, P < 0.01). This research is the first to report data on phenolic profile and total antioxidant capacity of Kelulut honey. Our data suggest that Kelulut honey has prominent medical properties and could be exploited as a natural nutraceutical to treat free radical associated diseases.
LWT - Food Science and Technology
Volume 89, March 2018, Pages 1-9
• LC-MS identified several phenolic compounds in stingless bee honey.
• Stingless bee honey had the highest anti-oxidant performance.
• Polyphenol content of stingless bee honey was associated with antioxidant activities.
This study aims to examine and compare the phenolic profile and antioxidant properties of two kinds of honey: Stingless bee honey (Kelulut honey) and Tualang honeys. Using liquid chromatography mass spectrometry, a total of eighteen phenolic acids and flavonoids have been identified in Kelulut honey samples. The phenolic content and total antioxidant capacity (three assays: DPPH, ABTS, and ORAC) for Kelulut and Tualang honeys were evaluated and statistically estimated. There was a significant (p < 0.05) phenolic content (228.09 ± 7.9–235.28 ± 0.6 mg gallic acid equivalent per kg) and flavonoid content (97.88 ± 10.1–101.5 ± 11.4 mg catechin equivalent per kg) in Kelulut honey samples. The Kelulut honey samples have demonstrated a significantly stronger antioxidant capacity than Tualang honey samples. The correlations between antioxidant results and polyphenols content were found to be statistically significant (P < 0.05, P < 0.01). This research is the first to report data on phenolic profile and total antioxidant capacity of Kelulut honey. Our data suggest that Kelulut honey has prominent medical properties and could be exploited as a natural nutraceutical to treat free radical associated diseases.
Sunday, November 05, 2017
Review of Medicinal Uses of Honey
Towards a better understanding of the therapeutic applications and corresponding mechanisms of action of honey
Environ Sci Pollut Res Int. 2017 Nov 3
Honey is a bee-derived supersaturated solution composed of complex contents mainly glucose, fructose, amino acids, vitamins, and minerals. Composition of honey may vary due to the difference in nectar, season, geography, and storage condition. Honey has been used since times immemorial in folk medicine and has recently been rediscovered as an excellent therapeutic agent.
In the past, honey was used for a variety of ailments without knowing the scientific background and active ingredients of honey. Today, honey has been scientifically proven for its antioxidant, regulation of glycemic response, antitumor, antimicrobial, anti-inflammatory, and cardiovascular potentiating agent. It can be used as a wound dressing and healing substance. Honey is different in color, flavor, sensory perception, and medical response. Apart from highlighting the nutritional facts of honey, we collected the finding of the published literature to know the mechanism of action of honey in different diseases.
This review covers the composition, physiochemical characteristics, and some medical uses.
Environ Sci Pollut Res Int. 2017 Nov 3
Honey is a bee-derived supersaturated solution composed of complex contents mainly glucose, fructose, amino acids, vitamins, and minerals. Composition of honey may vary due to the difference in nectar, season, geography, and storage condition. Honey has been used since times immemorial in folk medicine and has recently been rediscovered as an excellent therapeutic agent.
In the past, honey was used for a variety of ailments without knowing the scientific background and active ingredients of honey. Today, honey has been scientifically proven for its antioxidant, regulation of glycemic response, antitumor, antimicrobial, anti-inflammatory, and cardiovascular potentiating agent. It can be used as a wound dressing and healing substance. Honey is different in color, flavor, sensory perception, and medical response. Apart from highlighting the nutritional facts of honey, we collected the finding of the published literature to know the mechanism of action of honey in different diseases.
This review covers the composition, physiochemical characteristics, and some medical uses.
Friday, November 03, 2017
Bee Venom Acupuncture May Help Treat Chronic Pain
Suppressive Effects of Bee Venom Acupuncture on Paclitaxel-Induced Neuropathic Pain in Rats
Toxins 2017, 9(11), 351
Paclitaxel, a chemotherapy drug for solid tumors, induces peripheral painful neuropathy. Bee venom acupuncture (BVA) has been reported to have potent analgesic effects, which are known to be mediated by activation of spinal α-adrenergic receptor.
Here, we investigated the effect of BVA on mechanical hyperalgesia and spinal neuronal hyperexcitation induced by paclitaxel. The role of spinal α-adrenergic receptor subtypes in the analgesic effect of BVA was also observed. Administration of paclitaxel (total 8 mg/kg, intraperitoneal) on four alternate days (days 0, 2, 4, and 6) induced significant mechanical hyperalgesic signs, measured using a von Frey filament. BVA (1 mg/kg, ST36) relieved this mechanical hyperalgesia for at least two hours, and suppressed the hyperexcitation in spinal wide dynamic range neurons evoked by press or pinch stimulation.
Both melittin (0.5 mg/kg, ST36) and phospholipase A2 (0.12 mg/kg, ST36) were shown to play an important part in this analgesic effect of the BVA, as they significantly attenuated the pain. Intrathecal pretreatment with the α2-adrenergic receptor antagonist (idazoxan, 50 µg), but not α1-adrenergic receptor antagonist (prazosin, 30 µg), blocked the analgesic effect of BVA.
These results suggest that BVA has potent suppressive effects against paclitaxel-induced neuropathic pain, which were mediated by spinal α2-adrenergic receptor.
Thursday, November 02, 2017
Propolis as a Remedy for Toe Nail Fungus
5. Propolis solution
The more you apply the alcohol solution of propolis on the affected areas, that is, of course, better. You will be pleasantly surprised how fast will be the effect of the propolis. But note that even with the disappearance of the external manifestations of the disease, treatment should continue for some time. This completely eliminates the possibility of re-emergence of the fungus.
The more you apply the alcohol solution of propolis on the affected areas, that is, of course, better. You will be pleasantly surprised how fast will be the effect of the propolis. But note that even with the disappearance of the external manifestations of the disease, treatment should continue for some time. This completely eliminates the possibility of re-emergence of the fungus.
Wednesday, November 01, 2017
Vietnamese Propolis May Help Treat Cancer
A surprise discovery: Vietnam’s propolis can help treat cancer
VIETNAM.NET
Biologically, propolis is a mixture of resins and secretions from the honey bee’s salivary gland, which is flexible and glutinous at high temperatures but hard and fragile at low temperatures.
Studies have found more than 149 compounds and 22 different types of minerals in propolis. These compounds have many biological activities: antioxidant, antimicrobial, antiviral, anti-fungal, anti-inflammatory and anti-cancer.
Most of the products made of propolis take full advantage of its antibacterial characteristics. Bees use propolis to stabilize the structure of the nest, reduce vibration, mend tears and cracks of the nest. This strengthens the defense of the nest by blocking the entrance, prevents parasites and invasive bacteria, restricts the development of bacteria, fungi, and prevents any possibility of spreading harmful disease.
The best known propolis products available in the market are from South American countries, including Green Propolis which has very strong antibacterial activity, used to prevent flu.
Vietnam’s propolis has weaker antioxidant and antimicrobial activity. However, Vietnam’s propolis has very strong anti-cancer activity, especially pancreatic cancer.
Mai and her co-workers found that the propolis of Apis mellifera, which is raised in Vietnam, has flavonoid and polyphenol compounds much lower than the Brazilian honey bee, by 3-10 times.
Therefore, Vietnam’s propolis has weaker antioxidant and antimicrobial activity. However, Vietnam’s propolis has very strong anti-cancer activity, especially pancreatic cancer...
Tuesday, October 31, 2017
Honey Producers Stick Together in Manuka Trademark Fight
HELEN KEMPTON, Mercury, October 31, 2017
A NATIONAL campaign spearheaded by a honey producer in Tasmania’s North-West is gaining wings as beekeepers band together to stop New Zealand producers trademarking the word Manuka.
Manuka honey, derived from the tea tree (leptospermum) native to Tasmania, can retail for as much as $120 a jar and is deemed a health product because of its antibacterial properties.
There are five commercial Manuka honey producers in Tasmania and a number of smaller operations that provide them with the liquid gold.
In response to the trademark application in NZ, the Australian honey industry has collectively formed the Australian Manuka Honey Association to formally oppose any attempts to monopolise international naming or market rights...
A NATIONAL campaign spearheaded by a honey producer in Tasmania’s North-West is gaining wings as beekeepers band together to stop New Zealand producers trademarking the word Manuka.
Manuka honey, derived from the tea tree (leptospermum) native to Tasmania, can retail for as much as $120 a jar and is deemed a health product because of its antibacterial properties.
There are five commercial Manuka honey producers in Tasmania and a number of smaller operations that provide them with the liquid gold.
In response to the trademark application in NZ, the Australian honey industry has collectively formed the Australian Manuka Honey Association to formally oppose any attempts to monopolise international naming or market rights...
Monday, October 30, 2017
Nutritional and Medicinal Properties of Honey
Honey - ancient, golden wonder
Sunday, October 29, 2017
HONEY is the world's oldest natural unprocessed sweetener. It's a power bank of health benefits and a key to health and wealth. If you're not taking full advantage of the nutritional and medicinal properties of honey, it's time to begin because honey is a remarkable healing agent for all sorts of ailments.
A quick search on its health benefits shows it contains flavonoids, antioxidants which help reduce the risk of some cancers and heart disease as well as reduce ulcers and other gastrointestinal disorders. Honey is known for its anti-bacterial, anti-fungal properties for healing since ancient times. The Greeks, Romans, and Egyptians documented the healing properties of honey as early as Aristotle's mention of it in 384 BC. Even King Solomon said in the Bible: "My son, eat thou honey, for it is good."
Even doctors apply honey on wounds to speed up healing and diabetics use honey as their sugar replacement...
Sunday, October 29, 2017
HONEY is the world's oldest natural unprocessed sweetener. It's a power bank of health benefits and a key to health and wealth. If you're not taking full advantage of the nutritional and medicinal properties of honey, it's time to begin because honey is a remarkable healing agent for all sorts of ailments.
A quick search on its health benefits shows it contains flavonoids, antioxidants which help reduce the risk of some cancers and heart disease as well as reduce ulcers and other gastrointestinal disorders. Honey is known for its anti-bacterial, anti-fungal properties for healing since ancient times. The Greeks, Romans, and Egyptians documented the healing properties of honey as early as Aristotle's mention of it in 384 BC. Even King Solomon said in the Bible: "My son, eat thou honey, for it is good."
Even doctors apply honey on wounds to speed up healing and diabetics use honey as their sugar replacement...
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