Detection of Phytoconstituents in Column Fractions of
n-Hexane Extract of Goldcrest Honey Exhibiting Anti-Helicobacter pylori
Activity
Arch Med Res, 2012 May 3
BACKGROUND AND AIMS:
Alternative therapy for Helicobacter pylori eradication from
natural products is gaining much attention. This study sought to isolate and
characterize the fraction responsible for the antibacterial activity in
Goldcrest (GC) n-hexane extract.
METHODS:
Thin-layer chromatography (TLC) of the extract was carried
out on Silica gel plates to determine the presence of chemical compounds, which
were separated and partially purified by column chromatography. The obtained
fractions GCCL, GCF2, GCF3 and GCF4 were tested for anti-H. pylori activity
using the broth microdilution method. Volatile compounds in the active
fractions were identified by gas chromatography-mass spectrometry (GC-MS)
analysis. MINITAB was used for statistical analysis at 95% confidence interval.
RESULTS:
The best antibacterial activity was exhibited by GCF3 (5
mg/mL), which was composed of many compounds with known antimicrobial and
antioxidant properties. A total of 16 volatile compounds were identified from
fractions GCF2, GCF3 and GCF4 into the following families; alcohol, ketone,
aliphatic acid, benzene compound, hydrocarbon, furan and pyran derivatives.
CONCLUSIONS:
The demonstration of antibacterial activity by the column
fractions of GC n-hexane extract may provide new lead molecules that could
serve as selective agents for H. pylori chemotherapy and control.
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