Chemistry & Biodiversity, Volume 10, Issue 4, pages 649–657, April 2013
Hitherto unknown biological properties and the chemical
composition of the essential oil isolated from propolis of Indian origin were
established. GC/MS Analysis of the essential oil revealed the presence of 32
constituents, of which ten were major compounds, nine had intermediate
contents, and 13 were minor compounds.
With the exception of six minor constituents, that could not
be identified, their identification was based on the comparison of their mass
spectra and Kovats retention indices with those listed in the NIST and Wiley
mass spectral libraries. Their structural assignment was confirmed by GC/MS
co-injection of the essential oil with authentic compounds. Quantification of
the components was done by GC-FID analyses. Moreover, the essential oil was shown
to possess repellent activity against the honeybee Apis florea. The activity
was found to be dose dependent. The average repellency (ΔR) increased with
increasing essential-oil concentration up to 24 μg/ml and remained constant for
the formulation with the higher concentration.
These findings established the chemical constitution of the
essential oil and might be useful to beekeepers for the improvement of the bee
management.
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