The Effects of Bee Pollen Extracts on the Broiler Chicken's
Gastrointestinal Microflora
Res Vet Sci, 2013 Aug;95(1):34-7
The aim of this study was to investigate the effects of bee
pollen ethanolic extracts on the in vivo gastrointestinal tract microflora
colonization of broiler chickens.
A completely randomized experiment based on six treatments
(different concentrations of bee pollen - 0, 5, 15, 25, 35 and 45gkg(-1) diet)
was used during 7weeks. The highest count of faecal Enterococci was found in
the experimental group with the addition of 15g of pollen (8.85±0.87log
CFUg(-1)) per 1kg of feed mixture. The highest count of Lactobacilli was
detected in the experimental group with 35g of pollen per 1kg of feed mixture
and the highest number of the Enterobacteriaceae genera count was found in the
control group (8.43±0.15log CFUg(-1)).
Moreover, the MALDI TOF MS Biotyper identified the following
genera: Escherichia coli, Proteus mirabilis, Klebsiella oxytoca, as well as
Lactobacillus acidophilus, L. crispatus, L. fermentum and L. salivarius from
the Lactobacilli group and Enterococcus avium, E. casseliflavus, E. cecorum, E.
faecalis, E. faecium, E. gallinarum, E. hirae and E. malodoratus from the
Enterococci group. Additionally, the in vitro antimicrobial activities of
pollen against five bacteria species isolated from gastrointestinal tracts of
chickens were tested.
The best antimicrobial effect of the pollen extract was
detected against K. oxytoca.
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