Biol Pharm Bull, 2013;36(6):1032-5
Caffeic acid phenethyl ester (CAPE) is a component of
honeybee hives with various beneficial properties. Tissue factor (TF), the key
trigger of thrombosis, is expressed in human endothelial cells.
This study was designed to investigate whether CAPE modulates
TF expression in human aortic endothelial cells (HAECs). Western blots and
real-time polymerase chain reactions were performed. CAPE (10(-7)-10(-5) m)
inhibited tumor necrosis factor (TNF)-α induced endothelial TF protein
expression by 2.1-fold at 10(-5) m (p < 0.0001). Similarly, TF surface
activity was reduced (p < 0.02). In contrast, TF mRNA expression, TF
promoter activity, and mitogen-activated protein (MAP) kinase activation
remained unaltered.
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