Comparative analysis of antioxidant activity of honey of
different floral sources using recently developed polarographic and various
spectrophotometric assays
Volume 30, Issue 1, May 2013, Pages 13–18
Hydrogen peroxide scavenging (HPS) activity of honey of
different floral sources and its constituents such as predominant honey
flavonoids, phenolic acids, amino and organic acids, and carbohydrates have
been assessed by direct current (DC) polarographic assay. The assay was based
on decrease of anodic current of hydrogen peroxide complex, formed in alkaline
solution, at the potential of mercury dissolution. High correlations between
honey HPS activity, its total phenolic content (FC-GAE), antioxidant activity
measured by four standard methods (DPPH, TEAC, FRAP and ORAC), and also the
relative antioxidant capacity index, were obtained. Statistical evaluation by
ANOVA and F-test further confirmed the assay validity.
The results for individual compounds showed that HPS
activity of honey reflects an integrated action of a wide range of
constituents, both phenolics and non-phenolics.
The polarographic assay applied is a fast, reliable and low
cost alternative to spectrophotometric antioxidant assays commonly applied in
analysis of honey and can serve as an indicator of honey quality.
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