Cryo Letters. 2015 Jul-Aug;36(4):243-251.
BACKGROUND: Sugars are the energetic source for sperm to maintain the metabolic process, and the antibiotics slow down sperm degradation.
To study the effects of rosemary honey as energy source and cryoprotectant in combination with garlic as a natural antibiotic on the quality of ram spermatozoa upon cooling.
MATERIALS AND METHODS:
The ejaculates from three rams were evaluated at different times during cooling to determine its post-dilution quality.
Glycerol and dimethylformamide in conjunction with honey and garlic significantly improve the survival of spermatozoa.
The addition of honey and garlic reduces sperm deterioration when stored at 4 degree C.