Cryo Letters. 2015 Jul-Aug;36(4):243-251.
BACKGROUND: Sugars are the energetic source for sperm to maintain the metabolic process, and the antibiotics slow down sperm degradation.
OBJECTIVE:
To study the effects of rosemary honey as energy source and cryoprotectant in combination with garlic as a natural antibiotic on the quality of ram spermatozoa upon cooling.
MATERIALS AND METHODS:
The ejaculates from three rams were evaluated at different times during cooling to determine its post-dilution quality.
RESULTS:
Glycerol and dimethylformamide in conjunction with honey and garlic significantly improve the survival of spermatozoa.
CONCLUSION:
The addition of honey and garlic reduces sperm deterioration when stored at 4 degree C.
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