Journal of Diabetes Investigation
Accepted Article (Accepted, unedited articles published online and citable.
Oxidative stress has a key role in the pathogenesis of diabetes. Propolis and its constituents have a wide range of medicinal properties against oxidative stress. In this study we evaluated antioxidant effects of ethanolic extracts of propolis (EEP) on kidney in diabetes mellitus rats.
Materials and Methods
Forty male Wistar rats were randomly divided into the following five groups: control, diabetes mellitus, diabetes mellitus with vehicle-treated, diabetes mellitus with propolis-treated (100 mg/kg), and diabetes mellitus with propolis-treated (200 mg/kg). Diabetes mellitus in rats was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Diabetic groups were treated with vehicle or ethanolic extracts of Iranian propolis for 6 weeks. Serum concentration of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were measured.
The results showed that Iranian propolis significantly inhibit body weight loss in diabetes mellitus rats. The propolis extracts significantly reduced serum glucose levels and kidney weight in diabetes mellitus rats (p < 0.001). Moreover, significantly reduced the MDA content, increased the activity of SOD and GPx (p < 0.001) along with the total antioxidant activity in the kidney tissue of diabetes mellitus rats. In the kidney of the diabetes mellitus and vehicle group, the glomerular basement membrane (GBM) thickness and glomerular area (GA) were significantly increased. Treatment of diabetes mellitus rats with the propolis extract significantly reduced the GBM thickness and glomerular area.
The present study results showed that the Iranian propolis extract could enhance the antioxidant levels and histopathological in the kidney of rat. Final Results showed that the most of favorable effects of the propolis is mediated by a reduction of blood glucose levels in diabetic animals.