Saturday, March 01, 2008

Bee Venom Peptides Could be Drug Targeting Delivery System

Preparation and In Vitro Tumor Cells Selectivity of Sterically Stabilized Immunoliposomal Peptides in Bee Venom
Yao Xue Xue Bao, 2007 Nov;42(11):1201-5

Abstract: Recently the use of peptides in bee venom (PBV) for cancer therapy has attracted considerable attention.

In this study, the sterically stabilized liposomal PBV (PBV-SL) was prepared using soybean phosphatidylcholine, cholesterol, and cholesterol-PEG-COOH. The humanized antihepatoma disulfide-stabilized Fv (hdscFv25) was coupled to sterically stabilized liposomes using the N-hydroxysuccinimide ester method. The hdscFv25-immunoliposomes (SIL[hdscFv25]) were immunoreactive as determined by ELISA assay. SIL[hdscFv25] showed higher tumor cells selectivity. PBV-SIL[hdscFv25] can kill SMMC-7721 cells in vitro with higher efficiency than non-targeted liposomes. Whereas cytotoxicties were compared for Hela cells, no significant differences was observed between PBV-SIL[hdscFv25] and PBV-SL.

Sterically stabilized immunoliposomal peptides in bee venom could be one drug targeting delivery system.

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