Methods to Extract Adenosine Phosphates from Royal Jelly Studied

Extraction and Ultra-Performance Liquid Chromatographic Analysis of Adenosine Phosphates in Royal Jelly
Se Pu (Chinese Journal of Chromatography), 2008 Nov;26(6):736-9

Several different extraction procedures including perchloric acid extraction, boiling water extraction and boiling magnesium sulfate solution extraction were studied for the extraction of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) from the royal jelly.

Among these methods, the extraction with 5% perchloric acid at below 4 degrees C was the optimum extraction method. A simple, fast and sensitive ultra-performance liquid chromatographic (UPLC) method was developed for the determination of ATP, ADP and AMP in royal jelly…

This method was successfully applied to the analysis of some royal jelly samples from beekeepers and markets for the investigation of distribution of ATP, ADP and AMP in royal jelly samples.