Propolis Decrease Diet-Induced Hepatic Steatosis in Mice
Int. J. Morphol, 2010, vol.28, n.1, pp. 75-84
Nonalcoholic fatty liver disease (NAFLD) covers a wide spectrum of injuries ranging from simple steatosis to cirrhosis and hepatocellular carcinoma. Its main risk factors are disorders associated with metabolic syndrome (MS).
Propolis, a resinous substance produced by Apis mellifera to protect is hive, has demonstrated a hepatoprotective effect. Thus, the aim of this study was to evaluate the effect of Chilean propolis on development of nonalcoholic hepatic steatosis in C57BL/6J mice exposed to Paigen atherogenic diet.
Twenty eight mice C57BL/6J were divided four groups: 1, balanced diet (CH); 2, hypercholesterolemic diet (HD); 3, HD diet supplemented with 10 mg/kg/day of propolis (LP); 4, HD diet supplemented with 40 mg/kg/day of propolis (HP). After 16 weeks of treatment was determined glucose, total cholesterol and triglycerides concentrations and alanine aminotransferase (ALT) activity. The liver tissue was fixed in 10% buffered formalin solution, embedded in paraplast and stained with hematoxylin-eosin.
No differences was detected in glucose and triglycerides concentrations, contrasting with total cholesterol levels between group fed with a balanced diet (CH) and feed with atherogenic diet D12336 (HD, LP and HP). In the same way, it was noted differences in ALT activity between groups, standing out the reduction in propolis supplemented groups (LP and HP).
Likewise the histoarchitecture of HD group, showed simple steatosis, inflammatory cell infiltration and inflammatory foci in hepatic lobule. This characteristics show a gradual decrease in LP and HP groups.
In conclusion, the Chilean propolis analyzed decreases hepatic steatosis induced by atherogenic diet in C57BL/6J mice. However, the involved mechanisms in this hepatoprotective activity must be identified.