Growth Inhibition by Caffeic Acid, One of the Phenolic
Constituents of Honey, in HCT 15 Colon Cancer Cells
Scientific World Journal, 2012 Apr 29
Previous work from our laboratory showed that the mechanism
of crude-honey induced apoptosis in colon cancer cells. Since phenolic
constituents of honey were attributed to its apoptosis-inducing ability, we
studied caffeic acid, one of the phenolic constituents of honey, induced effect
on colon cancer cells.
Antiproliferative effect of caffeic acid was estimated using
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. MTT
assay signified the antiproliferative nature of caffeic acid against the HCT 15
colon cancer cells.
A time-dependent inhibition of colony formation was evident
with caffeic acid treatment. Cell-cycle analysis of caffeic acid- (CA-) treated
cells indicated increasing accumulation of cells at sub-G(1) phase.
Photomicrograph images of treated cells showed membrane blebbing and cell
shrinkage. Yo-pro-1 staining of caffeic-acid-treated cells confirmed apoptosis
in dose- and time-dependent manner. Increasing ROS generation and reduction in
the mitochondrial membrane potential were also accompanied in the caffeic
acid-induced apoptosis.
This work will promote caffeic acid as a likely candidate in
the chemoprevention of colon cancer.
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