Comparison of the Antimicrobial Effect of Egyptian Propolis
vs New Zealand Propolis on Streptococcus mutans and Lactobacilli in Saliva
Oral Health Prev Dent, 2012;10(2):155-60
Purpose: To evaluate the antimicrobial effect of Egyptian
propolis vs New Zealand propolis on Streptococcus mutans and lactobacilli in
saliva.
Materials and Methods: The strains used for the experiment
were isolated from 12 patients having a high caries index. The ethanolic
extract (EEP) of pure Egyptian propolis was obtained by dissolving 20 g of
propolis in 70% aqueous ethanol to a final volume of 100 ml. The commercial New
Zealand propolis, combined with antibacterial agents, was an ethanolic extract
of propolis in lozenge form; this was dissolved in distilled water to obtain an
EEP. The EEP was further fractioned using a liquid-liquid extraction technique
with hexane and chloroform solvents. The antimicrobial properties of the two
propolis types and their fractions on Streptococcus mutans and lactobacilli
were examined separately by determining minimal inhibitory concentration (MIC)
and minimal bactericidal concentration (MBC). Twelve clinical isolates were
obtained from the collected saliva of all patients, one (Streptococcus mutans
and Lactobacilli) from each patient, for susceptibility testing.
Results: The MIC values of the New Zealand propolis were
lower than the MIC values of the Egyptian propolis, indicating that the New
Zealand propolis and hexane fractions (H-fr) in general had stronger
antimicrobial effects. In addition, its antimicrobial action was greater on S.
mutans than on lactobacilli, except with H-fr they were the same.
Conclusion: The commercial New Zealand propolis hexane
fraction had the strongest antimicrobial action. The EEP had a more potent
antimicrobial effect on S. mutans than on lactobacilli.
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