Propolis Suppresses CdCl-Induced Cytotoxicity of COS7 Cells
through the Prevention of Intracellular Reactive Oxygen Species Accumulation
Biol Pharm Bull, 2012;35(7):1126-31
Propolis is a natural product made by honeybees and contains
various compounds, including flavonoids, amino acids and fatty acids. These compounds
are considered to have antiviral, antibacterial and antioxidative properties.
On the other hand, cadmium (Cd), an industrial and environmental pollutant,
preferentially accumulates in the kidney and induces kidney injury.
We previously reported that exposure to CdCl-induced cell
death though intracellular reactive oxygen species (ROS) generation in kidney
tubule epithelial COS7 cells. Here, we investigated whether propolis extracts
suppress CdCl-induced cytotoxicity.
Predictably, pretreatment with propolis extracts
significantly suppressed CdCl-induced cytotoxicity and intracellular ROS
generation. Propolis extracts not only showed superoxide dismutase and
antioxidative activities, but also increased the expression of heme oxygenase-1
(HO-1), an antioxidative enzyme. Moreover, we determined the involvement of
hypoxia inducible factor-1α in propolis extract-derived HO-1 induction.
We demonstrate for the first time the utility of propolis
for Cd-related COS7 cytotoxicity, and these novel findings are considered to
contribute to the control of ROS-derived disorders.
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