Consumption of Bee Pollen Affects Rat Ovarian Functions
J Anim Physiol Anim Nutr (Berl), 2012 Nov 9
http://onlinelibrary.wiley.com/doi/10.1111/jpn.12013/abstract
The aim of this study was to examine possible effects of bee
pollen added to the feed mixture (FM) on rat ovarian functions (secretion
activity and apoptosis). We evaluated the bee pollen effect on the release of
insulin-like growth factor I (IGF-I) and steroid hormones (progesterone and
estradiol), as well as on the expression of markers of apoptosis (Bcl-2, Bax
and caspase-3) in rat ovarian fragments. Female rats (n = 15) were fed during
90 days by FM without or with rape seed bee pollen in dose either 3 kg/1000 kg
FM or 5 kg/1000 kg FM. Fragments of ovaries isolated from rats of each group
(totally 72 pieces) were incubated for 24 h. Hormonal secretion into the
culture medium was detected by RIA. The markers of apoptosis were evaluated by
Western blotting. It was observed that IGF-I release by rat ovarian fragments
was significantly decreased; on the other hand, progesterone and
estradiol secretion was increased after bee pollen treatment at dose
5 kg/1000 kg FM but not at 3 kg/1000 FM. Accumulation of Bcl-2 was increased by
bee pollen added at 3 kg/1000 kg FM, but not at higher dose. Accumulation of
Bax was increased in ovaries of rats fed by bee pollen at doses either 3 or
5 kg/1000 kg FM, whilst accumulation of caspase-3 increased after feeding with
bee pollen at dose 5 kg/1000 kg FM, but not at 3 kg/1000 kg FM. Our results
contribute to new insights regarding the effect of bee pollen on both secretion
activity (release of growth factor IGF-I and steroid hormones progesterone and
estradiol) and apoptosis (anti- and pro-apoptotic markers Bcl-2, Bax and
caspase-3). Bee pollen is shown to be a potent regulator of rat ovarian
functions.
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