Attenuation of CCl4-Induced Oxidative Stress and
Hepatonephrotoxicity by Saudi Sidr Honey in Rats
Evid Based Complement Alternat Med, 2013;2013:569037 Epub
2013 Feb 24
The present study was undertaken to investigate the possible
protective effect of Saudi Sidr honey (SSH) on carbon tetrachloride (CCl4)
induced oxidative stress and liver and kidney damage in rat. Moreover, the
antioxidant activity and the phenolic and flavonoidal contents were determined.
The hepatorenal protective activity of the SSH was
determined by assessing biochemical, hematological, and histological
parameters. Serum transaminases, ALP, GGT, creatinine, bilirubin urea, uric
acid, and MDA level in liver and kidney tissues were significantly elevated,
and the antioxidant status of nonprotein sulfhydryls, albumin, and total
protein levels in liver and kidney were declined significantly in CCl4 alone
treated animals.
Pretreatment with SSH and silymarin prior to the administration
of CCl4 significantly prevented the increase of the serum levels of enzyme
markers and reduced oxidative stress. SSH also exhibited a significant
lipid-lowering effect and caused an HDL-C enhanced level in serum.
The histopathological evaluation of the liver and kidney
also revealed that honey protected incidence of both liver and kidney lesions.
Moreover, SSH showed a strong antioxidant activity in DPPH and β
-carotene-linoleic acid assays. SSH was found to contain phenolic compounds.
Additionally, the SSH supplementation restored the hepatocytes viability
against 2',7'-dichlorofluorescein (DCF) toxicity in ex vivo test.
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