Polish Natural Bee Honeys Are Anti-Proliferative and
Anti-Metastatic Agents in Human Glioblastoma multiforme U87MG Cell Line
PLOS One
Honey has been used as food and a traditional medicament
since ancient times. However, recently many scientists have been concentrating
on the anti-oxidant, anti-proliferative, anti-inflammatory and other properties
of honey.
In this study, we investigated for the first time an
anticancer effect of different honeys from Poland on tumor cell line -
glioblastoma multiforme U87MG. Anti-proliferative activity of honeys and its
interferences with temozolomide were determined by a cytotoxicity test and DNA
binding by [H3]-thymidine incorporation. A gelatin zymography was used to
conduct an evaluation of metalloproteinases (MMP-2 and MMP-9) expression in
U87MG treatment with honey samples. The honeys were previously tested
qualitatively (diastase activity, total phenolic content, lead and cadmium
content). The data demonstrated that the examined honeys have a potent
anti-proliferative effect on U87MG cell line in a time- and dose-dependent
manner, being effective at concentrations as low as 0.5% (multifloral light honey
- viability 53% after 72 h of incubation). We observed that after 48 h,
combining honey with temozolomide showed a significantly higher inhibitory
effect than the samples of honey alone. We observed a strong inhibition of
MMP-2 and MMP-9 for the tested honeys (from 20 to 56% and from 5 to 58%
compared to control, respectively).
Our results suggest that Polish honeys have an
anti-proliferative and anti-metastatic effect on U87MG cell line. Therefore,
natural bee honey can be considered as a promising adjuvant treatment for brain
tumors.
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