Royal Jelly Enhances Migration of Human Dermal Fibroblasts with Decreased Levels of Triglycerides And Cholesterol in In Vitro Wound Healing Model
FASEB J, 2010 24:922.6
Oral administration of royal jelly (RJ) promotes the wound healing in diabetic mice. Concerns have arisen about the efficacy of RJ on wound healing process of normal skin cells.
In this study, a wound was created by scratching normal human dermal fibroblasts, one of major cells involved wound healing process. 0.1, 1.0 or 5 ug/ml RJ were promptly treated up to 48 hrs, and migration was analyzed by the closure of wound margin. Furthermore, altered levels of lipids, which is recently reported to be responsible for wound healing process, were analyzed by HPTLC.
Migration of fibroblasts was peaked up to ~24hr after wounding. RJ treatment significantly accelerated migration of fibroblasts in a dose dependent manner at 8hr. Although RJ accelerated migration of fibroblasts at either 20 hr or 24hr, the efficacy of RJ was less portent than at 8hr. Among various lipid classes of fibroblasts, the levels of triglycerol and cholesterol were significantly decreased with 5 ug/ml RJ.
In spite of a dose-dependent increase of sphinganine, no altered level of sphingosine, ceramides and glucosylceramide was noted with any concentration of RJ.
We demonstrate that RJ enhances migration of fibroblasts with altered levels of various lipids in the wound healing process.