Adjuvanticity of Epimedium Polysaccharide-Propolis Flavone
On Inactivated Vaccines Against AI and ND Virus
Int J Biol Macromol, 2012 Aug 27
The purpose of this research was to compare the activities
of different dose of epimedium flavone-propolis flavone adjuvant (EPA). The
inactivated avian influenza (AI) and Newcastle disease (ND) vaccine contained
three dose of EPA were prepared. In AI vaccine vaccination experiment, three
hundred 14-day-old chickens were randomly divided into 6 groups and inoculated
with three EPA-AI vaccines taking oil adjuvant (OA), non-adjuvant (NA) vaccines
and physiological saline as controls, repeated at 28-day-old. The lymphocyte
proliferation and serum antibody titer were determined. In ND vaccine
vaccination experiment, three hundred 14-day-old chickens were grouped, treated
with three EPA-ND vaccines, and determined same to AI vaccine vaccination
experiment, at 42-day-old the chickens were challenged with NDV. On day 15
after challenged, the immune protective effect was observed. The results showed
that EPA could significantly promote lymphocyte proliferation and enhance serum
antibody titer against AI and ND, and reduce the morbidity of chickens
challenged with NDV after vaccinated with ND vaccine, especially the effect of
medium dose was better than that of non-adjuvant and oil adjuvant. These
results indicated that EPA could enhance the immune effect of inactivated AI vaccine
and ND vaccine and would be expected as a new-type adjuvant.
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