Apimedica: Honey Has Significant Effect on Growth of Burn Wound Microbes - 蜂蜜与抗生素抗烧伤感染细菌作用的比较研究

Apimondia Apimedica-Apiquality International Forum

Oct. 22-25, 2012

Zhenjiang, China

Study of Antimicrobial Effect of Honey in Comparison to the Antibiotics on the Microbes Isolated from Infected Burn Wounds

Quart ul Ain Zahra (QAZ), Zahid Mehmood Nagra (ZMN), Tamsil Ahsan (TA), M. Yousaf (MY) and Samina Qamer (SA)** 

Department of Zoology, Government College University, Faisalabad(QAZ,SA), Department of Plastic Surgery, Punjab Medical College, Faisalabad (ZMN),  Pathology Lab Allied hospital, Faisalabad (TA,MY),Pakistan

This study was conducted to evaluate the antimicrobial effect of Apis mellifera fresh honey on organisms isolated from infected burns in comparison to the antibiotics used in treatment of burn infection, and to evaluate the effects produced when bee honey is added to antibiotic discs.

Thirty patients with burn infection (2-4 weeks old) were selected for this study. The collected blood specimens from the burn wounds were cultured on different media such as CLED, Blood, SS and MacConkey agar and Nutrient agar. Four major bacterial strains including E-coli, Klebsiella spp. Staphylococcus aureus and Pseudomonas aeruginosa were isolated and Antibiotic sensitivity pattern was measured against these microorganisms by using following 3 antibiotics i.e, Amikacin (AK), Zinacef (CXM), Linozolid (LZD).

The results show that honey has a significant effect on the growth of Pseudomonas aeruginosa (61%) followed by E.coli (55%), Staphylococcus aureus (56%), and Klebsiella spp (25%) as compared to the antibiotics alone.

** corresponding author: Dr Samina Qamer

e-mail: saminabee@yahoo.com

蜂蜜与抗生素抗烧伤感染细菌作用的比较研究

Quart ul Ain Zahra, Samina Qamer*（巴基斯坦）

(Department of Zoology, Government College University, Faisalabad, Pakistan )

本研究通过比较西方蜜蜂采集的新鲜蜂蜜与治疗创面感染的抗生素抗烧伤感染细菌的作用，进而评估 蜂蜜加入到抗菌药物中的效果。筛选了30 名创面感染期在2~4 周的病人，采集创面血液样本，并在CLED、血液、生长抑制素、马康基氏琼脂和营养琼脂等不同培养基中培养。共分离出大肠杆菌、克雷伯杆菌、金 黄色酿脓葡萄球菌和绿脓假单胞菌四种主要的菌株，并利用丁胺卡那霉素、呋肟头孢菌素和利奈唑胺测定 对这四种菌株的抗生素敏感性。结果表明，蜂蜜具有显著的抗菌效果，以单独使用抗生素疗效为参比，蜂 蜜对绿脓假单胞菌、大肠杆菌、金黄色酿脓葡萄球菌和克雷伯杆菌的抗菌效果分别达到了61%、55%、56% 和25%。

关键词：蜂蜜；微生物；丁胺卡那霉素；烧伤创面