Proteomic Analysis of the Royal Jelly and Characterization
of the Functions of its Derivation Glands in the Honeybee
J. Proteome Res, Article ASAP
To identify candidate royal jelly (RJ) proteins that might
affect the physiologic status of honeybee colony members, we used shotgun
proteomics to comprehensively identify the RJ proteome as well as proteomes of
the hypopharyngeal gland (HpG), postcerebral gland (PcG), and thoracic gland
(TG), from which RJ proteins are assumed to be derived.
We identified a total of 38 nonredundant RJ proteins,
including 22 putative secretory proteins and Insulin-like growth factor-binding
protein complex acid labile subunit. Among them, 9 proteins were newly
identified from RJ. Comparison of the RJ proteome with the HpG, PcG, and TG
proteomes revealed that 17 of the 22 putative secretory RJ proteins were
derived from some of these glands, suggesting that the RJ proteome is a
cocktail of proteins from these three glands. Furthermore, pathway analysis
suggested that the HpG proteome represents the molecular basis of the extremely
high protein-synthesizing ability, whereas the PcG proteome suggests that the
PcG functions as a reservoir for the volatile compounds and a primer pheromone.
Finally, to further characterize the possible total RJ
proteome, we identified putative secretory proteins in the proteomes of these
three glands. This will be useful for predicting novel RJ protein components in
future studies.
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